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허준,변성진 경희대학교 부설 조경계획연구소 1996 造景論叢 Vol.8 No.1
At the time of research, middle school students gave rise to lots of social problems. It might be remedied by the proper education and leisure in a way. It was the physical activity that they liked most to any other. But the lack of physical education and facility could not be adequate to their needs. So this research was carried out to employ the urban park in the physical education and leisure space. And the followings were the things which were connected with the solution. It was not what all kinds of park should be physical park but the park which had completed facilities or not could be used as it, because there was a physical park in the current urban park act. A self-governing body had to realize the lack of physical education space and facility so as to establish an ordinance. And the problems which could be occurred during school hours could be solved by grouping schools which lay adjacent to a park. So walking and shuttle bus could help it.
Leucine-C¹⁴에 依한 Puromycin이 白鼠顎下腺의 蛋白合成에 미치는 影響에 關한 自記放射法的硏究
許濬 최신의학사 1971 最新醫學 Vol.14 No.11
Male Balb C/Strain were used in all experiment. They ranged in weight from 18 to 23 gm. Puromycin dihydrochloride was injected intraperitoneally in aqueous solution, in a concentration of 2mg. /ml. , which had been neutralized with naoh. Two doses of 80mg/kg body weight each were given on day 1 intervals. Following the last injection, 3 pairs each were sacrificed on days 4, 6, 10 and 15. Thirty minutes prior to sacrifice 49c/gm body weight of leucine-C" (Specific Activity 278mc/mM) were injected. For the light microscopic studies, tissue were fixed 2% paraformaldehyde and sections were stained with toluidine blue o. The purpose of the present study was to determine, by means of quantitative autoradiography, the effect of puromycin on the protein synthesis of submandibular gland cells. 1. Following administration of puromycin to mice for 10 days, many of the acinar cells are lack the normal basophilia, increase of the vacuoles and the nuclear pyknosis. 2. We have observed that the protein synthesis as expressed by the incorporation of leucine-C" into submandibular gland cells is suppressed during first 4 days after the puromycin injections, but was significantly suppressed by 10 days. The grain number in puromycin-treated animals increased to a level similar to that of the controls by 15 days. 3. It is concluded that the synthesis of overall cytoplasmic proteins is temporarily impaired after puro-?mycin injections in submandibular gland cells of mice.