The expression patterns of RANKL and OPG in murine tooth eruption

Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.

해외직접투자 정보공시와 주가반응

정경만,문대진,차영진 釜山外國語大學校國際通商硏究所 1997 國際經營論集 Vol.12 No.-

ORP profile을 이용한 연속 회분식 반응기(Sequencing Batch Rector)에서 무산소공정 추론

심문용,부경민,임정훈,우혜진,김창원 한국환경과학회 2004 한국환경과학회지 Vol.13 No.3

The SBR(Sequencing Batch Reactor) process is ideally suited to treat high loading wastewater due to its high dilution rate. SBR operates by a cycle of periods consisting of filling, reacting, settling, decanting and idling. The react phases such as aeration or non-aeration, organic oxidation, nitrification, denitrification and other biological reactions can be achieved in a reactor. Although the whole reactions can be achieved in a SBR with time distributing, it is hard to manage the SBR as a normal condition without recognizing a present state. The present state can be observed with nutrient sensors such as NH₄^(-)-N, NO₂^(-)-N, NO₃^(-)-N and PO₄^(3-)-P. However, there is still a disadvantage to use the nutrient sensors because of their high expense and inconvenience to manage. Therefore, it is very useful to use common on-line sensors such as DO, ORP and pH, which are less expensive and more convient. Moreover, the present states and unexpected changes of SBR might be predicted by using of them. This study was conducted to get basic materials for making an inference of SBR process from ORP(oxidation reduction potential) of synthetic wastewater. The profiles of ORP, DO, and pH were under normal nitrification and denitrification were obtained to compare abnormal condition. And also, nitrite and nitrate accumulation were investigated during reaction of SBR. The bending point on ORP profile was not entirely in the low COD/NOx ratio condition. In this case, NOx was not entirely removed, and minimum ORP value was presented over -300mV. Under suitable COD/NOx ratio which complete denitrification was achieved, ORP bending point was observed and minimum ORP value was under -300mV. Under high COD/NOx ratio, ORP bending point was not detected at the first subcycle because of the fast denitrification and minimum ORP value was under -300mV at the time.

Impedance를 이용한 전도성고분자 센서의 가스감응특성

이경문,유준부,이병수,전희권,이상태,이덕동,허증수 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

스키상해에 관한 실태 조사 연구

김정주,민경두,이재문 경희대학교 체육과학연구소 2001 體育學論文集 Vol.29 No.-

As the number of people who enjoy skiing are increasing, it has become more important to work out a countermeasure for prevention of injuries. To prevent accidents, finding out the causes and analyzing them is more meaningful than post-accidental treatment. This study is based on records of 494 injured people at H ski resorts. From December, 2000 to March, 2001 this research and analysis was undertaken in detail by classifying the situation and causes at the accidents, into personal, material and environmental factors. The results from which following conclusion were made. First of all, after analyzing the situation of the injured people, the facts that most of the accidents occurred on weekends and holidays, when most students and salarymen went skiing and that the injures were usually in the form of a slight contusion were found out. The major cause was that people ignored the safety rules and descended immoderately. But skiers that used a certain ski resort regularly showed lower occurrence of injuries. For these reasons in order to reduce the rate of accidents happening skiers should be tested by a ski instructor and then should be taught at a right level. Ski-related organizations should inform skiers with facts that are commonly misunderstood through seminars and training courses. The lack of facilities for beginners should be improved and resorts for group training courses should be built separately so that beginners can prevent ahead of time, form getting hurt in a disorderly situation .

샌드위치 패널심재로 활용을 위한 VA/VeoVa 개질 다공성 경량골재 콘크리트의 특성

노정식,문경주,소양섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

Sandwich panel made by foamed styrene and polyurethane has been used generally in the construction area because of the high thermal conductivity and light weight. But, They occur harmful gases to both bodies and environments in the high temperature over 50℃. So, the purpose of this study is to investigate the physical properties of light-weight panel using the non-structural lightweight aggregate as a part of the substitution of foamed styrene and polymer-cement ratio as 5, 10, 15% and the filling ratio of continuous void as 50, 60% on the strength of polymer-modified sandwich panel core. From the result, we could know that the compressive and flexural strength of the sandwich panel core using non- structural lightweight aggregate and polymer dispersion such as SBR, St/BA-1 and St/BA-2 tended to be increased with an increase in the polymer-cement ratio and the filling ratio of continuous void.

중추성 요붕증이 동반된 랑게르한스 세포 조직구증 1예

김진호,문준성,문선중,이지은,최재원,은미정,천경아,조인호,윤지성,원규장,이경희 신덕섭,이형우 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Central diabetes insipidus (DI) is a syndrome characterized by thirst, polydipsia and polyuria. Langerhans cell histiocytosis is one of the etiologies of DI. Recently we experienced a central DI associated with Langerhans cell histiocytosis. The 44 years old female patient complained right hip pain polydipsia and polyuria. We carried out water deprivation test. After vasopressin injection, urine osmotic pressure was increased form 109mOsmol/Kg to 327mOsmol/Kg (300%). Brain MRI showed a thickened pituitary stalk and at hot bone CT.CT guided biopsy revealed abnormal histiocytes proliferation and abundant lymphocytes, The final diagnosis was central DI associated with systemic Langerhans cell histiocytosis invading hip bone, L-spine and pituitary stalk. Desmopressin and etoposide chemotherapy were performed to the patient.

전위된 관절내 원위 요골의 관절경을 이용한 고정술

이원용,박병문,임대의,송경섭,홍정우 대한골절학회 2003 대한골절학회지 Vol.16 No.3

목적: 전위된 관절내 원위 요골 골절에 관절경을 이용한 정복술과 경피적 K 강선 및 외고정기기를 이용한 수술법의 결과를 알아보고자 함이다. 대상 및 방법: 2001년 1월부터 8월까지 수술한 11명 12예를 대상으로 하였으며, 평균 추시 기간은 14개월 이었다. 골절의 형태는 AO 분류에 의하면 1예는 B3형, 4예는 C2형, 7예는 C3형 골절이었다. 전예에서 2.7㎜ 관절경을 이용하였으며 추가적인 관혈적 정복은 시행하지 않았다. 경피적 K 강선 고정술 후 외고정 기기를 사용하였다. 관절내 골연골 골편을 제거하였으며 추가적인 수근 골간 인대와 삼각 섬유 연골 손상의 적극적인 처치는 없었다. 고정물 제거 시기는 경피적 K 강선은 술후 4주, 외고정 기기는 7주에 시행하였다. 결과: 평균 수근 관절의 신전 및 굴곡은 정상의 72%였다. 평균 요측 길이는 12.3 ㎜, 전방경사는 5.7°, 척측 경사는 22.4°였다. 평균 관절면의 불일치는 1㎜였다. 술후 구획 증후군의 발생은 없었으며 치료 중 골절 함몰 소견은 없었다. 임상적으로 Gartland와 Werley의 평가에 의하면 8예에서 우수, 3예에서 양호, 1예에서 보통의 결과를 얻었다. 결론: 관절내 분쇄 골절의 치료에서 관절경을 이용한 관절면 정복술은 수술적 연부 조직을 최소화하면서 관절면을 비교적 정확하게 정복함으로 관절면의 불일치를 감소시켜 손상 후 좋은 결과를 얻을 수 있는 효과적인 방법으로 사료된다. Purpose: To investigate the result of arthroscopically assisted reduction of intraarticular fracture of distal radius with percutaneous K-wires and external fixation. Materials and Methods: We reviewed 12 intraarticular distal radial fractures of 11 patients treated arthroscopically assisted reduction in Orthopedic Department of Sung-Ae hospital between January, 2001 and August, 2001. The mean length of follow-up was 14 months. Analysis of them revealed that B3 was 1 case, C2 4 cases and C3 7 cases according to the AO classification. All cases were treated by 2.7 ㎜ arthroscopic devices and percutaneous K-wires pining with external fixationg, but additionally invasive reductional technique was not used. We removed the osteochondral flap but not treated. The K-wires were removed at 4 weeks, external fixation was 7 weeks, respectivley. Result: The mean active range of movement in the affected wrist was total arc of the flextion-extension 72% of the opposite side. Radiographically mean volar tilt, radial inclination and radial length compartment syndrome and fractures collapse were not occured during follow-up period. According to the Gartland and Werley demerit-point system, eleven cases were a excellent or good one was a fair result. Conclusion: Arthroscopically assisted fixation is a useful method for reducing the soft tissue injuies and preventing the articular surface incogruency by anatomically reduction un cases of intraarticular comminution.

조대술에 의한 하마종의 치료

강동균,황경문,김은정,김현정,김영진,남순현 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

하마종은 구강저에 발생하는 점액 남종으로 주로 설하선과 관련된다. 즉, 하마종은 설하선 분비도관의 파열로 인하여 타액이 분비도관에서 빠져나와 주위의 연조직 내에 저류됨으로써 발생하는 가성 낭으로 정의 되며, 원인은 설하선 도관의 파열이나 폐색이다. 하마종의 임상소견은 청색의 투명한 종창이 구강저의 소대를 중심으로 편측으로 발생하며, 무통성이며, 촉진시 파동성을 나타낸다. 조직소견은 결합 조직 내 와동이 형성되지만, 낭 벽은 상피세포가 아닌 육아조직으로 구성되는 가성 낭을 관찰할 수 있다. 하마종의 첫 번째 치료법으로는 하마종의 내벽이 구강점막의 일부가 되도록 만들어 주는 조대술을 고려할 수 있다. 본 증례는 특징적인 하마종의 임상소견을 보이는 환아에게 gauze-packing등을 동반한 조대술을 이용하여 치료한 증례로써 양호한 치료결과를 보여 보고하고자 한다. Ranula is a mucocele which occurs at the mouth floor and mostly related to sublingual gland. In other words ranula is definded as a pseudocyst which occurs as the secretion duct of sublingual gland is destructed there as the saliva from the the secretion duct flows out and retention in the soft tissue. The cause of ranula is destruction or obstruction of the duct. The clinical findings of ranula is a painless, unilateral bluish transparent swelling around the frenum and shows fluctuation when palpated. Histological finding represent a formation of cavity inside the connective tissue. but a pseudo-cyst can be seen which the wall of the cyst is composed of granulation tissue rather than epithelial cells. The first treatment of ranula can be considered as marsupialization. which induces the inner wall of the Ranula to be a part of oral mucosa. This case report shows a treatment of marsupialization with gauze packing in a young patient representing a clinical finding of characteristic ranula.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.