Aptamer‑linked immobilized sorbent assay for detecting GMO marker, phosphinothricin acetyltransferase (PAT)

Woo‑Ri Shin,Mun‑Jong Lee,SEKHONSIMRANJEET SINGH,Ji Hun Kim,Sun Chang Kim,Byung‑Kwan Cho,Ji‑Young Ahn,Yang‑Hoon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Development of genetically modified crops has rapidly increased in last few years. The most widely grown GM crops express genes that confer herbicide tolerance and insect resistance. Detection system of GM crops is important for safety evaluation before its consumption. Objective The purpose of this research is to detect GM crops, especially PAT, in food-samples. Results The bar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E. coli. The high-affinity PAT-specific single-stranded DNA (ssDNA) aptamers were obtained from a random DNA library. MOE docking study was performed to identify the potential binding region of the selected aptamers on PAT. Aptamer-linked immobilized sorbent assay (ALISA) method was used to detect PAT. Conclusion We screened aptamer against PAT for developing an efficient detection method. The selected PAT specific aptamers, HRPA-05 and HRPA-07, showed the distinct target binding behaviors, and detected PAT protein by aptamer-linked immobilized sorbent assay method with high efficiency and selectivity.

전남 진도 지역어의 단모음 실현 양상

하영우 ( Ha Young-woo ),신우봉 ( Shin Woo-bong ) 고려대학교 민족문화연구원 2018 民族文化硏究 Vol.79 No.-

이 연구는 전남 진도 지역어의 단모음 실현 양상과 특징을 살펴보는 것을 목적으로 한다. 이를 위해 20명의 진도 토박이 화자를 대상으로 단모음 음성 자료를 구축하여 성별, 세대에 따른 단모음의 음성적 특징을 분석하였다. 실험 결과, 전남 서남부에 위치한 진도 지역어의 단모음 체계는 /ㅣ, E, ㅡ, ㅓ, ㅏ, ㅜ, ㅗ/의 7모음 체계임을 확인하였다. 전남 방언은 대개 /ㅚ/, /ㅟ/를 단모음으로 보았으나, 진도 지역 장년층은 두 모음을 대부분 이중모음으로 조음하였다. 또한, 청년층은 모두 두 모음을 완벽하게 이중모음으로 실현하였다. 한편, 진도 지역어의 단모음 조음 위치를 분석한 결과 세대간 차이가 관찰되었다. 진도 지역 장년층 화자와 달리 청년층 화자는 /ㅡ/의 전방화, /ㅜ/의 전방화, /ㅗ/의 고모음화, /ㅓ/의 후설 저모음화로 이어지는 후설 고·중모음의 연쇄적인 조음 위치 변화가 나타났다. 청년층의 단모음 음가 변화는 진도 지역은 물론 여타의 방언권에서도 나타나는 표준어 수렴 현상의 하나이다. 이상의 실험 결과를 종합해 보면, 진도 지역 화자는 세대에 따른 단모음 체계에 차이는 없지만 개별 단모음의 음성 실현 양상에는 차이가 있다. 본고는 기존 연구와 달리 음성 자료의 직접적 분석을 바탕으로 진도 지역어의 단모음 체계를 구명한 점과 세대에 따른 조음 위치적 변화를 포착하였다는 데 의의가 있다. The aim of this study was to examine the acoustic characteristics of the monophthongs in the Jeonnam-Jindo dialect. For this purpose, the experiment was conducted and data was gathered from Jindo dialect speakers in their 10s and 5,60s. The data were analyzed on the basis of the subjects’ generation, gender. The result of production experiment was that Jindo dialect speakers have same vowel system between generations: 7 vowel system (/i, E, a, □, i, u, o/). The older generation of Jindo speakers mostly pronounce /ㅚ/ and /ㅟ/ as monophthong irrespective of sex and the young generation of Jindo speakers entirely pronounce /ㅚ/ and /ㅟ/ as monophthong. Also, we found that /Щ/, /u/ moved toward front and /o/ moved toward high in the vowel space from the Jindo speakers in their older to those in their young. And /Λ/ showed a shift in the vowel space from the back to the open vowel. The results revealed a chain-like shift in the high and mid back vowels. It is assumable that the degree of standardization appearing in the place of articulation on vowel is quite high in the Jindo dialect. Previous studies discussed the Jindo vowel system on the basis of the observations made by the researchers. The current study, on the other hand, is significant in that it investigated sound changes in vowel production throughout the vowel system and confirmed the articulation place of vowel using phonetic methods.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

고주파 가열에 따른 반응고 알루미늄합금의 미세조직 특성

신평우,이정환,이상용,노진호,이영선 國立 昌原大學校 産業技術硏究所 1997 産技硏論文集 Vol.11 No.-

Semi-solid forming is relatively new process for forming alloys in the semi-solid condition into near net produce. It relies on the behaviour of semi-solid slurrys in which the solid exists in the form of non-dendritic, spheroidal particles in a liquid matrix. Powder of the Al-20Si-5Fe-2Ni alloy was manufactured by spray forming and powder metallurgy, one of the rapid cooling method, and its microstructure became fine with several ㎛ size through the extrusion process. Spray forming and powder metallurgy are a rapid solidification process in which capable of producing materials consisted of very fine, uniform particles. Spray forming can be described as incorperating the advantages of powder metallurgy without disadvantages of degassing and consolidation. In this paper, the microstructural evolution of the P/M, S/F Al-20Si-5Fe-2Ni alloys and wrought Al 2024 alloys were investigated various heating conditions by high frequency induction heating system.

남성화 암쥐의 모리스 수중 미로 수행력 : 성에 따른 공간기억력과 테스토르테론 효과의 차이

신성현,박제민,정우영,박문일,서유나,김성곤,강철중 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objectives : It is widely accepted that gonadal steroids are involved in organization and activation of sexual dimorphism in the brain areas related with spatial leaming. This study aimed to test the following hypothesis : 1) spatial memory is affected by sex, 2) this difference is related with brain differentiation by exposure to testosterone in the neonatal period, and/or 3) the sexual difference of spatial memory is the result of the interaction between organization of sexual dimorphism in the brain and the circulating testosterone after growing up. Methods : Testosterone enanthate 100 ㎍, ('androgenized') orvehicle ('control') was randomly administered to 28 female and 25 male rat pups within 24 hour of birth. The escape latencies (EL) in Morris water maze were measured in 15 and 16 week of age with vehicle ('baseline') and with 10 μmol/kg of testosterone ('with testosterone'), respectively. Each session Consis-ted of 10 trials, and means of the last 5 trials were analyzed. Results : The baseline EL of the males were significantly shorter than females regardless ofneonatal exposure to testosterone. With testosterone, EL of the androgenized females were significantly shorter than control females and comparable to those of males. Conclusion : Neonatal exposure to testosterone in female rats may develop a tesosterone-dependent male-like spatial leaming system.

분편 인플루엔자백신(split influenza vaccine)의 임상효과 및 면역원성에 관한 연구

우흥정,김동림,정희진,천병철,이주연,안정배,김지희,박찬,신영규,김우주,김민자,박승철 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.1

목적 : 아단위 인플루엔자 백신 접종 후 백신의 인플루엔자 예방효과, 인플루엔자 방어 항체형성, 인플루엔자 백신의 안전성을 조사하고자 하였다. 방법 : 총 571명을 대상으로 인플루엔자 백신 접종을 하였고, 이들 접종자에서 인플루엔자 양질환의 이환을 조사하여 인플루엔자 백신의 인플루엔자예방효과를 알아보았고, 백신의 접종 전 및 접종 4주 후 혈청에서 혈구응집억제물(Hemagglutination Inhibition : HAI) 항체 검사를 실시하여 백신의 방어항체생성을 조사하였고, 백신의 안전성을 알아보기 위해 백신접종 후 1주일 이내의 부작용을 조사하였다. 결과 :백신 접종군과 백신 비접종군에서 인플루엔자 양 질환의 이환을 조사한 결과 접종군 28.35%, 비접종군 35.88%으로 나왔으며, p 값이 0.001로 통계적으로 접종군에서 유의하게 낮았고, 인플루엔자 양 질환의 예방 효과는 20.97%를 보였다. 백신의 방어항체 형성의 평가를 위해 유럽의 인플루엔자 백신 허가 기준을 조사하였는데 B/Guangdong/5/94균주의 백신접종 후 항체가 40이상의 비율을 제외한 다른 기준은 모두 만족 시켰다. 부작용은 전체 조사자 521명중 149명(29%)으로 주로 접종 부위의 국소 부작용을 호소했고, 전신 부작용은 2% 내외였으며 특별히 심각한 부작용은 발견되지 않았다. 결론 : 분편 인플루엔자 백신은 인플루엔자양질환의 예방과 방어항체생성에 효과 있으며 안전한 것으로 사료된다. Background : The safety and effectiveness of influenza vaccine are well known in developed country. The influenza vaccination has been recommended as one of the tentative immunization schedule for indicated persons since 1997 in Korea. But there are still no available data about them, even though nearly 5 million doses of influenza vaccine were used in 1997-1998 season. So it is immediately needed to investigate the safety. efficacy and immunogenicity of influenza vaccine among Korean. Methods : We studied the clinical efficacy of influenza vaccine by monitoring Occurrence of influenza-like illness in influenza risk group(vaccination ; 300, non-vaccination; 215) from December in 1997 to March in 1998. We used the split quadrivalent influenza vaccine containing 15 microgram of hemagglutinin of A/Beijing/262/95(HlNl), A/Wuhan/359/95(H3N2), B/Mie/1/93 and B/Guangdong/5/94. Hemagglutination inhibition(HA1) antibody titers were determined before immunization and 1 months after vaccination And we evaluated adverse effect of influenza vaccination at 7 days after vaccination. Results : Influenza vaccination was associated with si@icant reductions in influenza-like spptoms(vaccination group; 28.35%, non-vaccination group, 35.88%, p=0.001). The preventive effect of influenza-like i3lne.s among influenza risk goup was 20.97%. And immunogenicity of influenza A and B exceeded all of the European licensure criteria for immunogenicity except postvaccination proportion of titers 240 of B/Guangdong/5/94 strain. And the adverse effects were mainly local injection site problem and no serious adverse effect was noted. Conclusion : Split influenza vaccine is safe, inmunogenic and eff'tive in influenza risk group in Korea.

이성대상포진의 증상에 따른 안면신경마비의 정도와 예후

신시옥,송창근,이동욱,최영석,진흥률,신정우,윤희석 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.1

연구목적: 이성대상포진에 동반되는 안면신경마비는 Bell's palsy와는 달리 예후가 더 나쁘고 그 마비가 완전히 회복되지 않는 경우가 더 많다.본 연구에서는 이성대상포진에서 발현되는 증상들의 유무가 치료 전 안면신경마비의 정도 및 치료 후 예후와 상관관계가 있는지를 관찰하여 예후의 예측인자에 대해 분석하였다. 재료 및 방법: 지난 1992년 1월부터 1999년 6월까지 이성대상포진으로 진단받고 본원에 입원하여 고용량 부신피질호르몬제와 항바이러스제인 acyclovir의 병합투여로 동일한 치료를 받은 30명의 환자를 대상으로 후향적으로 의무기록을 분석 및 고찰하였으며, 치료 전 안면마비의 정도와 치료 후 마비의 회복 정도를 이통, 이명, 난청, 현훈 등의 증상의 유무와 치료 시작 시기, 그리고 안면신경의 electroneuronography(ENoG) 값에 따라 비교하였다. 결과: 이성대상포진수포가 안면신경마비 보다 먼저 발현된 경우에 안면신경마비의 치료 전 grade가 통계적으로 유의하게 좋았다(p<0.05).이통, 청력손실, 그리고 현훈의 유무에 따른 안면신경마비의 정도는 상관관계가 없었고 예후에도 차이가 없었다.이명의 유무에 따른 치료 전 안면신경마비의 정도는 상관관계가 없었으나 치료 후 완전회복율은 이명이 있었던 군에서 통계적으로 유의하게 높았다(p=0.003<0.05).완전회복군과 불완전회복군 사이의 치료 전 ENoG의 수치는 통계적으로 유의하게 예후와 상관이 있었다(p=0.037<0.05). 결론: 좀더 많은 수의 환자를 연구에 포함하여 증상에 따른 예후의 차이를 연구하는 것이 필 요하며 또한 ENoG 값이 10%에서 40% 사이인 환자들의 치료 방법에 대한 고려와 이성대상포진수포가 안면신경마비보다 먼저 생긴 환자에서 치료 전 마비정도가 경미했던 이유에 대한 연구가 필요할 것으로 생각된다. Purpose: Herpes zoster oticus generally has a poor prognosis, leaving many patients with permanent facial nerve dysfunction, compare to Bell's palsy.Acyclovir and prednisolone have been widely used for the treatment of herpes zoster oticus.This retrospective study was conducted to suggest the prognostic factors according to various symptoms. Materials and Methods : Clinical studies were retrospectively performed on 30 patients with herpes zoster oticus who were treated with acyclovir and prednisolone in the Chungbuk National University Hospital between 1991 and 1999.The authors analyze the pre-treatment grade and prognosis of facial nerve palsy according to symptoms(the existence of otalgia, tinnitus, hearing loss, and vertigo), the treatment onset, and electroneuronography ( ENoG ). Results : Of thirty patients who have been diagnosed as herpes zoster oticus, there is no significant difference in the pre-treatment grade of facial nerve palsy according to the existence of vesicle, otalgia, tinnitus, vertigo, and hearing loss.Of 25 patients who have facial nerve palsy, the recovery from the palsy is complete in 14 patients(56%).There is no significant difference in the pre-treatment grade of facial nerve palsy according to the existence of otalgia, tinnitus, vertigo, and hearing loss.The pre-treatment grade of facial palsy is better when the vesicles precede facial in the group with tinnitus than without tinnitus.A significant difference of the prognosis according to the onset of treatment and other symptoms are not found.ENoG is better in the group that facial nerve palsy is completely recovered than incomplete recovery group. Conclusion : Reliable prognostic factor according to symptoms is not found, ENoG is the only prognostic factor.

입원환자의 편집성증상에 관한 임상적 고찰

신영우 大韓神經精神醫學會 1982 신경정신의학 Vol.21 No.1

The author studied 133 neuropsychiatric inpatients with paranoid symptoms and 144 neuropsychiatric inpatients without paranoid symptoms, who had been managed at the neuropsychiatric unit of Kyung-Hee University Hospital during a 18 month period. Data were analyzed and the following results were obtained. 1. Of the 277 patients admitted to the unit, 133 (48.0%) were classified as paranoid on the basis of the criteria using derived from Paranoid Symptoms of Hamilton's Depression Scale and Present State Examination. 2. Among the 133 paranoid patients, 52 patients (39%) were diagnosed as schizophrenia, 25(19%) affective disorder, 11(8%) organic brain syndrome and 11(8%) neurosis. 3. Paranoid symptoms were noticed in all of paranoia and acute paranoid reaction,85% of schizophrenia,76% of affective disorders,36%of organic brain syndrome and 14%of neurosis. 4. The modes of treatment in paranoid patients were antipsychotics(68%), electro-convulsive therapy(22%)and antidepressants(12%),while those in non-paranoid patients were anxiolytics(48%), psychotherapy(25%), antipsychotics(16%) and antidepressants(12%). 5. Basic variables,such as age, sex, education, occupation and religion were not significantly correlated with either paranoid or non-paranoid symptoms.

결정화 공정을 위한 L-ornithine aspartate의 용해도에 관한 연구

신동명,김용욱,김우식 慶熙大學校 1998 論文集 Vol.27 No.-

Study on the solubility of L-ornithine aspartate with temperature, alcohol, additive was invescated. Generally, the solubilities of amio acids are effected by orniganic solvent, so the solubility of L-ornithine aspartate was represented to reduce by adding alcohol. The solubility of L-ornithine aspartate with temperature incressed at methanol volume ratio 40%, but was slightly effected at methanol volume ratio 60% and 80%. The effect of aspartic acid was not signifigant, too.