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- 저자 : Sang Keun Lee,Young-Won Kim,Ho Won Kang,Won Tae Kim,Yong-June Kim,Seok-Joong Yun,Wun-Jae Kim,Sang-Cheol Lee (검색결과 8,781 건)
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뇌졸중 후 중추성 통증 환자에 대한 동서협진이 진통과 재활에 미치는 영향
이현종,김수영,이상훈,서동민,이두익,김건식,이재동,이윤호,양형인,박재경,최도영 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-
Purpose : In order to study the effectiveness of East-West pain treatment on central poststroke pain(CPSP), we evaluated its effect on alleviation of pain and rehabilitation of CPSP Patients who were treated with electroacupuncture and west pain treatment for four weeks. Methods : Twenty four patients diagnosed by their pain characteristics of central pain from stroke were treated with sympathetic nerve block, gabapentin, amitriptyline, and electroacupuncture for four weeks. Pain intensity through the visual analogue scale(VAS), and improvements of mobility and rehabilitation through the modified Barthel index(MBI) and Rankin scale(RS), respectively, before and after pain treatment were also assessed. Results : VAS pain scores were significantly improved from 7.7±1.7 to 4.4±2.0 with pain treatment(p<0.05). In accordance with improvement of pain scores, RS and MBI scores were also improved from 2.88±0.95 to 2.13± 1.01 and from 83.0± 16.9 to 94.7±9.5(p<0.05), respectively, with pain treatment(p<0.05). Conclusions : It was suggested that the active pain treatment was contributed to the rehabilitation of CPSP patients, resulting in improvement of quality of life of CPSP patients. Futhermore, East pain treatment in combination with West pain treatment may be useful modality to alleviate CPSP.
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소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구
이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-
The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).
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Sang-keun Kim,Myung-hun Lee,Man-hee Rhee 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2
on the Effects of Biomedicinal Agents on Serum Concentration of Ca2+, P andALP Activity in Osteoporosis-Induced RatsSang-keun Kim, Myung-hun Lee1 and Man-hee Rhee2College of Veterinary Medicine, Chungnam National University, 220 Gungdong, Yusong-Gu, Daejeon 305-764, Korea1National Veterinary Research and Quarantine Service, Anyang 430-824, Korea2College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, KoreaReceived April 3, 2003 / Accepted July 9, 2003J. Vet. Sci. (2003), 4(2), 151-154JOURNAL OFVeterinaryScience*Corresponding author: Sang-keun Kim Dept. of Vet. Med., Chungnam National University, Daejeon 305764, Korea Tel: +82-42-821-6754, Fax: +82-42-821-6754 E.-mail: kskkim@cnu.ac.kr
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추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정
이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5
본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.
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이승훈,배성민,문근석,유미진,이지훈 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.2
The purpose of this study was to define the relationship between housewife's practical physical education activity, leisure satisfaction and life satisfaction. For achieving the purpose, an analysis was made over the impact of attendance at public sports center on leisure satisfaction, the influence of attendance at public sports center on life satisfaction, the relationship of leisure satisfaction to life satisfaction for those who joined public sports center, and the impact of an experience of taking part in public sports center on life satisfaction. The instruments used in this study were Beard and Ragheb(1980)'s Leisure Satisfaction Scale(LSS) and Diener, Emmoson, Larsen, and Griffin(1985)'s Satisfaction with Life Scale(SWLS) adapted for this study. The statistical technique employed to analysis of variance and multiple regression analysis. The findings of this study were as below: First, the housewives who attended public sports center was significantly different in leisure satisfaction from one another according to personal characteristics. A significantly better leisure satisfaction was expressed by the group that was less educated, was older, had less than 3,000 thousand won of family income, was in very good health, chose golf, or responded to seeking more meaningful life. For job, the unemployed group was slightly higher in leisure satisfaction than the employed. Second, the personal characteristics also made a significant difference to the life satisfaction of the housewives who attended public sports center. Especially, a significantly higher life satisfaction was shown by the group that was older, had less than 3,000 thousand won of family income, was in very good health, chose golf was a little higher in life satisfaction than the unemployed. But education level generated no significant difference. Third, there appeared a close relationship between the housewives' leisure satisfaction and life satisfaction and life satisfaction who attended public sports center. Fourth, when the experience of attending public sports center was considered, the housewives with more than 3-year experience, who weren't expected to drop out in the practical physical education program, expressed significantly higher leisure satisfaction and life satisfaction. As this study found that the group that had participated in practical physical education program in public sports center for more than 3 years expressed better life satisfaction, it can be concluded that practical physical education can serve to raise housewife's leisure satisfaction and life satisfaction who joins public sports center.
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이석희,이상은,박종근,문성배 부산대학교 사범대학 과학교육연구소 2001 科學敎育硏究報 Vol.28 No.-
The purpose of this study is to examine the effects of the application of experimental practices, descriptive tests, and research papers among the performance assessments on the students' accomplishment, their scientific research abilities and their attitudes to science in high schools. Two classes among the first grade ones were selected and assigned to the control group and the experimental group respectively. T-analysis of pre-test showed that the two groups have the same quality(p>.05). The questionnaire made by the Korean Educational Development Institute(KEDI) was used. Its reliability is .689. The experimental process was evaluated on the basis of the criterion table and reports. T-tes and ANOVA were enforced with the SPSS/WIN(ver10.0) for the purpose of analyzing the mean difference between the two groups. The results of this study are as follows. First, though the application of the performance assessments showed that there were little significant differences between the scientific research abilities of the experimental group with the experimental practice and those of the control group(p>.05), and the results of the pre-test and the post-test on the experimental group revealed that there were significant effects on improving the students' scientific research abilities(p<.05). Second, the result of the test using ANOVA at each research course factor showed that there was no significant difference at most research course factors that there were significant differences between the two groups in expanding the scientific research abilities of classification and measurement(p<.05). Finally, the results of the pre-test and the post-test by application of the performance assessments showed that they had significant effects on changing the students' attitude to science subject positively(p<.05). In addition, the results of the pre-testing and the post-testing on the sub-factors of the attitude related to science showed that there were significant changes in the concept of 'self-consciousness' and the attitude to learning(p<.05). In conclusion, the application of the performance assessments has positive effects on improving the high school students' scholastic achievement, scientific research abilities, and attitudes to related to science. Consequently, science teachers should be willing to use various performance assessments in the class.
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