競走馬에서의 藥物檢査

金尙塡 대한수의학회 1990 대한수의학회 학술대회발표집 Vol.1990 No.-

Diethyl maleate가 N-methyl-N'-nitro-N-nitrosoguanidine에 의해서 유발되는 랫드 위암 발생에 미치는 영향에 관한 병리학적 연구

박철범,이준섭,Park, Cheol-bom,Lee, Joon-sup 대한수의학회 1995 대한수의학회지 Vol.35 No.4

This study was carried out to investigate the effects of diethyl maleate(DEM) on the carcinogenesis of forestomach and pyloric glandular stomach in rats caused by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG). A total of 60 male 6-week-old Wistar rats were given twice intragastric injection of MMNG(200mg/kg BW), then were given diets containing 5% NaCl for 3 weeks until 4th week of the experiment. And then the animals of groups of 1 and 2 were placed on diets containing 0.2% DEM for 16 weeks until the end of 20 weeks of the experiment. On the other hand, the animals of groups of 3 and 4 were placed on basal diets for the same periods. The tissues of forestomach and liver of each group were frozen in liquid nitrogen and the activities of quinone reductase(QR) were determined by measurement of the dicoumarol-sensitive reduction of dichloro-indophenol by NADPH at 600nm. All rats were sacrificed at the end of 20 weeks of the experiment. Every animal was fasted for 24 hrs prior to sacrifice. The forestomach was fixed in 10% neutral phosphate buffered formalin for histology and the pyloric gland was fixed in sublimated formalin for immunohistochemistry of pepsinogen 1 altered pyloric gland(PAPG). The final body weight of the group given MNNG and treated with 5% NaCI and DEM was significantly decreased compared with that of the group 4(p<0.05). Food and water consumption rates were not significantly changed. The preneoplastic and neoplastic lesions of the forestomach given MNNG and treated with 5% NaCI and DEM were significantly increased compared to those of the group 4(p<0.0l). The incidence of PAPG in the groups treated with 0.2% DEM was significantly increased compared with that of the group 4(group 1:p<0.01, group 2:p<0.05). The activities of QR of forestomach in the groups treated with 0.2% DEM were significanitly increased compared with those of the group 4(p<0.001), but those of liver were not significant. These results indicate that DEM exert the enhancing effect of forestomach and glandular stomach carcinogenesis in rats pretreated with MNNG and NaCl.

고양이 하부요로(下部尿路) 질환(疾患)의 병리학적(病理學的) 소견(所見)에 관한 연구(硏究)

신사경,미야하라 카즈로,사투 모요요시,김상근,히로세 쓰네오,Shin, Sa-kyeng,Miyahara, Kazuro,Satu, Motoyosi,Kim, Sang-keun,Hirose, Tsuneo 대한수의학회 1995 대한수의학회지 Vol.35 No.4

The kidney, heart and lung were examined of cats fallen sick lower urinary tract disease(LUTD). There cases typically clinical findings were urinary retention, hematuria, dehydration, cytoplegia and uremia. It is believed that these histopathological and clinical findings resulted from the struvite crystal thrombus in ureter. But we have many factors of an unsolved problem on LUTD. Accordingly, this LUTD demands to process of pathological diposition.

한우 림프구에 대한 단일항체 생산 2. E-rosette형성 림프구에 대한 단일항체

송희종,운재호,김태중,Song, Hee-jong,Woon, Jae-ho,Kim, Tae-joong 대한수의학회 1995 대한수의학회지 Vol.35 No.4

A monoclonal antibody(MAb), KCT-23ER, with specificity for E-rosetted T cells of Korean native cattle was prepared by cell hybridization of myeloma P3/NS-1/1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with E-rosetted lymphocytes. The isotype of KCT-23ER to T lymphocytes was mouse $IgG_{2b}$. KCT-23ER was reacted with 53.6% to peripheral blood lymphocytes and with 67.8% to nylon wool nonadherent blood lymphocytes. And it was reacted with 72.2%, 59.2% and 35.3% to thymocytes, prescapular lymph node cells and splenocytes, respectively. Immunocytological reactive rates to E-rosetted and non-E-rosetted cells were 72.5% and 22.4%, respectively. These results indicated that KCT-23ER reacted to E-rosetted cells was one of the MAb for investigate of $CD_2$ receptor positive cell subset in the Korean native cattle.

한우 림프구에 대한 단일항체 생산 1. T 림프구에 대한 단일항체

송희종,운재호,김태중,채효석,허부홍,최민순,Song, Hee-jong,Woon, Jae-ho,Kim, Tae-joong,Chai, Hyo-seok,Hur, Boo-hong,Choi, Min-soon 대한수의학회 1995 대한수의학회지 Vol.35 No.4

This study was undertaken to develop the monoclonal antibody(MAb) for lymphocytes of Korean native cattle by the cell hybridization of myeloma P3/NS-1/ 1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with nylon wool column eluted peripheral T lymphocytes of Korean native cattle. The isotype of MAb KCT-14 against T lymphocyte was mouse $IgG_1$. KCT-14 positivity of mononuclear cells(MNC) from peripheral blood lymphocytes, nylon wool nonadherent and adherent-lymphocytes was 41.7%, 58.4% and 22.6%, respectively. And that of mesenteric lymph node-, spleen and thymus-MNC was 43.3%, 40.2% and 33.6%, respectively. Immunoperoxidase staining of frozen tissue sections showed that the MAb positive cells were located in the medulla of the thymus and in the paracortical area and the mantle zone of the germinal center in the lymph nodes. These results indicated that KCT-14 was one of the MAb for investigate of T lymphocyte subpopulations in the Korean native cattle.

국내 개의 리켓치아 감염에 관한 역학조사

김희선,강문일,이채용,김성호,Kim, Hee-sun,Kang, Mun-il,Lee, Chai-yong,Kim, Sung-ho 대한수의학회 1995 대한수의학회지 Vol.35 No.4

One hundred twelve domestic dog sera were collected from Kyonggi, Kanngwon, Cholla, and Kyongsang province. All the sera were examined by immunofluorescence antibody test(IFAT) against scrub typhus, murine typhus and spotted fever group rickettsia infection. The antibody prevalence was 5.40%(6/112) against scrub typhus. The sero-prevalence rates of scrub typhus were 16.30%(5/31) in Cholla and 5.90%(1/17)in Kyonggi areas. The prevalence of scrub typhus was higher in older dogs that were 6 years or more. The antibody prevalence was 8.04%(9/112) against murine typhus. The sero-positive rates to murine typhus were 5.88%(1/17) in Kyonggi, 8.33%(2/24) in Kangwon, 9.68%(3/31) in Cholla, and 7.50%(3/40) in Kyongsang areas, respectively. The sero-positive dogs to murine typhus were widely distributed throughout all age groups from 2 months to above 9 years old. The antibody prevalence was 16.96%(19/112) for spotted fever group rickettsial infection. The sero-positive rates of spotted fever group rickettsia were 11.76%(2/17) in Kyonggi, 12.90%(4/31) in Cholla, and 32.50%(13/40) in Kyongsang areas. The sero-positive dogs to spotted fever group rickettsia were observed at all age groups of dog. The sero-positive prevalence between sex showed higher in female than in male except for spotted fever group rickettsia infection.

국내 말로부터 비폐렴바이러스의 분리 및 면역원성에 관한 연구 III. 말 비폐렴바이러스의 실험동물에 대한 면역원성 조사

조길재,김봉환,소신희,이두식,Cho, Gil-jae,Kim, Bong-hwan,So, Sin-hee,Lee, Du-sik 대한수의학회 1995 대한수의학회지 Vol.35 No.4

The study was carried out to investigate the pathogenicity of EHV isolate to hamsters and mice and immunogenicity of experimentally produced. vaccine were evaluated in the horses. Hamsters infected. intranasally with $LC_1$ isolate showed symptoms of nasal discharge, conjunctivitis and body weight loss during the observation period of 12 days after infection, while only slight depression and body weight loss were noticed with mice infected with $LC_1$ indicating that hamsters are more susceptible to the virus. Antibody titer of mice and hamsters were gradually increased to highest level of 1:2560~10240, 1:640~1280, respectively, at 7~12 days post vaccination. Horses immunized against $LC_1$ killed vaccine reached to maximum antibody titer of 1:20480 around 4 weeks after 1st vaccination and declined after 12 weeks post vaccination. No significant antibody increase were detected after 2nd vaccination. Mean body temperature and mean total leukocyte counts remained within normal range and no adverse reaction were noticed after vaccination.

국내 말로부터 비폐렴바이러스의 분리 및 면역원성에 관한 연구 II. 국내 분리 말 비폐렴바이러스의 특성 및 면역원성 조사

조길재,김봉환,이두식,오문유,고미희,Cho, Gil-jae,Kim, Bong-hwan,Lee, Du-sik,Oh, Moon-you,Ko, Mi-hee 대한수의학회 1995 대한수의학회지 Vol.35 No.4

The study was carried out to characterize the properties of Korean isolates of EHV from aborted fetuses and determine envelope protein profiles. The results obtained were summarized as follows; 1. Two strains of EHV was isolated from 2 liver samples among 10 aborted fetuses from which the virus isolation was attempted. 2. Morphological and some enzymatic properties of the Korean isolates of EHV which was designated as $LC_1$ and $LC_2$ was identical to those of a reference strain of Australia-N of EHV-1. The Korean isolates of EHV could be propagated on ED cell culture and they formed typical plaques 1 to 2 days after infection in the ED cells from which typical cuboidal particles of 150~170 nm diameter herpesvirus were observed. The virus could be detected specifically from neucleus and cytoplasm of infected cells by flourescent antibody technique using FITC labelled anti-Aust IV(EHV-1) antiserum. The Korean isolates, $LC_1$ and $LC_2$ were specifically neutralized by anti Aust IV antiserum and reacted positively to CELISA. 3. The structural polypeptides of purified enveloped virions of $LC_1$ and $LC_2$ isolates of EHV were determined by SDS-polyacrylamide gel electrophoresis to identify the envelope glycoproteins. $LC_1$ and $LC_2$ strains revealed 14 glycoproteins ranging in molecular weight from 190 kD to 31 kD while 17 structural proteins of Aust IV(EHV-1), of which 14 were identical to those of $LC_1$ and $LC_2$, were identified. Upon immunoblotting by rabbit antiserum against EHV isolates and EHV-1(Aust IV), 4 immunogenic proteins of $LC_1$ and $LC_2$ were 135 kD, 88 kD, 64 kD and 59 kD, of which 135 kD, 88 kD and 64 kD proteins were also found in Aust IV(EHV-1).

국내 말로부터 비폐렴바이러스의 분리 및 면역원성에 관한 연구 I. 말 비폐렴바이러스(equine rhinopneumonitis virus)에 대한 혈청학적 역학조사

조길재,김봉환,이두식,신태균,양기천,임윤규,조성수,Cho, Gil-jae,Kim, Bong-hwan,Lee, Du-sik,Shin, Tae-kyun,Yang, Ki-chun,Lim, Yoon-kyu,Cho, Sung-soo 대한수의학회 1995 대한수의학회지 Vol.35 No.4

The present study was conducted to investigate the incidence of equine herpesvirus 1(EHV-1) infection in Korean horses by cell EUSA and serum neutralization test A significant level of antibodies to EHV-1 was detected from 539(82.8%) by cell EUSA and from 524 horses(80.5%) by SNT indicating that EHV-1 infection in Korean horses are widely spreaded throughout the country.

청서 시상하부의 growth hormone-releasing factor 및 somatostatin 면역반응신경세포의 분포

정영길,손화영,윤원기,김길수,원무호,류시윤,조성환,김무강,Jeong, Young-gil,Son, Hwa-young,Yoon, Won-kee,Kim, Kil-soo,Won, Moo-ho,Ryu, Si-yun,Cho, Sung-whan,Kim, Moo-kang 대한수의학회 1995 대한수의학회지 Vol.35 No.4

This study was attempted to investigate the topographical distribution, shape and immunoreactivity of growth hormone-releasing factor(GRF)- and somatostatin(SOM)-immunoreactive neurons in the hypothalamus of the Korean squirrels(Sciurus vulgalis coreae). For the light microscopical examination of immunohistochemistry, the brains were fixed with 4% paraformaldehyde solution by means of intracardiac perfusion. And the frozen sections($40{\mu}m$ thick) were stained immunohistochemically by ABC method. Distribution of GRF immunoreactive neurons($12-17{\mu}m$) was highest in the paraventricular nucleus, moderate in the periventricular and supraoptic nuclei, and low in the arcuate nucleus and lateral hypothalamic area. Their immunoreactive fibers were found very high in the median eminence, moderately in the supraoptic, paraventricular and periventricular nuclei, and low in the arcuate nucleus and lateral hypothalamic area. SOM immunoreactive perikarya($14-18{\mu}m$) were found moderately in the periventricular nucleus near the subependymal layer of the third ventricle, and low in the arcuate and suprachiasmatic nuclei. SOM immunoreactive fibers were found high in the median eminence, and moderately or low in the arcuate and periventricular nuclei.