競走馬에서의 藥物檢査

金尙塡 대한수의학회 1990 대한수의학회 학술대회발표집 Vol.1990 No.-

몇가지 자율신경성약물(自律神經性藥物)이 Reserpine 처리(處理) 병아리의 척출공장(剔出空腸) 내재성신경(內在性神經)에 미치는 영향(影響)

이창업,Lee, Chang Eop 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2

Pharmacological investigation was performed in chickens which were pretreated with reserpine. Transmural stimulations were given to the isolated jejunum of chickens and studied the responses and the effects of dibenamine, propranolol, atropine and tetrodotoxin on them. The results obtained were summerized as follows: 1. Three different patterns of response were obtained from the isolated jejunum of non-treated chickens after giving them transmural stimulation. The first pattern was contraction followed by relaxation, the second pattern was contraction only and the third pattern was relaxation only. 2. The transmural stimulation of the jejunum preparations evoked relaxation while the periarterial stimulation evoked contraction in the reserpinized chick. 3. The relaxation response to the transmural stimulation was not affected by the pretreatment with dibenamine, propranolol and atropine. 4. The relaxation response to the transmural stimulation was abolished by tetrodotoxin. The results obtained in these studies indicate that the relaxation response to the transmural stimulation is due to non-adrenergic inhibitory fibers.

세균성자궁질환(細菌性子宮疾患)에 있어서 Estrogen 이 미치는 영향(影響)에 관한 연구

오수각,옥종화,Oh, Soo Kak,Oak, Chong Wha 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.1

Estrous and non-estrous rabbits were inoculated with E. coli or Streptococcus pyogenes, and the mixture of the two organisms, and bacterial count and histopathological studies of uterine horns were made to observe the effects of estrogen on the resistance of the uterus to bacterial infection. The results obtained were summarized as followings; 1. Four hours after inoculation of bactoria into uterine horn, the number of organisms was significantly lower in estrous rabbits than in non-estrous regardless of the kind of organisms inoculated. 2. The highest reduction rate of the organisms among the three bacterial inoculation groups was found in estrous rabbits inoculated with E. coli, and the lowest reduction rate was with Streptococcus pyogenes. 3. Histopathological changes of uterine horns induced five days after bacterial inoculation were observed. In estrous rabbits, a mild inflammatory reaction was found in Streptococcus pyogenes group, but a slight inflammatory reaction and only a negligible inflammatory reaction were observed in mixed bacteria group, and in E. coli group respectively. In non-estrous group, however, a marked inflammatory reaction was observed in Streptococcus pyogenes group, a moderate inflammatory reaction and a slight inflammatory reaction were observed in the mixed bacterial group and E. coli group, respectively.

비환(鼻環)에 의한 돈폐충증(豚肺蟲症)의 예방(豫防)에 관한 연구(硏究)

임정택,윤화중,한방근,김영홍,Lim, C.T.,Yoon, H.J.,Han, B.K.,Kim, Y.H. 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.1

1. Of 4,112 earth worms, 3,480 were classified as Eisenia foetidae, and 3,277 of the species were infested with the larvae of Metastrongylus apri. 2. Cresol and $CaCN_2$ were found effective in the insecticidal test of earthworms. 3. The nose ring was effective, in pigs, to prevent digging and eating the earthworms and resulted a lower lungworm incidence in pigs. 4. The best place to put the nose ring was the nasal septum.

마우스의 실험적(實驗的) 고사리 중독(中毒)에 관한 병리학적(病理學的) 연구(硏究)

임병무,이호일,Rim, Byung Moo,Lee, Ho Il 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.2

In order to investigate the effects of bracken fern (Pteridium aquilinum) on animals, a total of 105 mice given a ration containing different percentage of bracken fern were studied pathologically during the experimental period of 90 days. The results obtained were as follow: 1. Mice in the group, given a ration containing 15% of bracken fern, showed 85% of death rate in 90 days, and mice in the group, given a ration containing 25% of it, showed 100% in 30 days 2. The experimental mice showed decreased myeloid cells and significant reduction in erythrocytes, leukocytes, and thrombocytes. 3. Severe congestion and hemorrhages were observed in lungs, liver, kidney, spleen, stomach, intestine, brain, adrenal and testis from the ones that died during the experimental period, but the necropsies of the latter period indicated mild chanties comparatively. 4. Mice in the group, given a ration containing 15% blacken fern showed a decreasing tendency in body weight.

닭순환임파구내에 출현하는 Azurophil 과립의 동태에 관한 전자현미경적 연구

김화식,Kim, Hwa Sik 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.2

With an effort to clarify the nature of the azurphil granules which occasionally occur in the circulating lymphocytes, these granules were investigated by examining smear of the peripheral blood of the chikens in various stage of the individual growth and after injection of methylene blue and gentian violet. In addition, the fine structure of these granules were also investigated. The results were: 1. These granules were first occurred in the lymphocytes just after their hatching (0.004%). The proportion of lymphocytes containing these granules were increased with their growth and in adult chicken its occurrence was higher than mammals. 2. Marked variations in its fine structure, particularly in its size and cotents, were noted but they were believed to belong to categories of lysosome of de Duve. 3. Lymphocytes containing azurophil granule were increased after injection of the non-immunogenic substances, such as gentian violet and methylene blue. 4. From the above results, chicken is bettor animal to study theme granules because of its higher occurrence. They are believed to have intimate relationship with bodily cellular reaction against the foreign materials because they are increased after non-immunogenic stimuli.

임신유우(姙娠乳牛)의 혈청중(血淸中) Vitamin E 농도(濃度)

용만중,Yong, Mahn Joong 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2

Blood serum from 30 pregnant cows, 6 non-Pregnant cows (one month after delivery), 6 sterile cows (ovarian cyst), and 6 heifers (16 months old) were analyzed for total vitamin E concentration by the ferric chloride-bipyridyl reaction. The pregnant group was determined periodically throughout pregnancy. The results obtained were as follows: 1. The mean value of serum vitamin E level in pregnant group was $0.651{\pm}0.068(SE)mg/100ml$, in non-pregnant group $0.647{\pm}0.119(SE)mg/100ml$, in sterile group $0.488{\pm}0.053(SE)mg/100ml$, and in heifer group $0.083{\pm}0.016(SE)mg/100ml$. Samples below 0.19mg/100ml were net observed and most of the pregnants (83.3%) showed the values of 0.40 to 0.99mg/100ml during pregnancy 2. The difference of vitamin E level between pregnant group and non-pregnant group was net significant, but these trio groups showed a significantly higher value than that of sterile group. On the other hand, in heifer group was recognized significantly lower value compared with adult groups. 3. The change of serum vitamin E level in pregnant group revealed the tendency of decrease as gestation progresses and significantly dropped at the eighth month, thereafter, the value was rose slightly at the tenth month of gestation but no differences were observed. The coefficient of correlation between months and serum vitamin E levels during pregnancy was r=-0.956(p<0.01), and a regression equation was Y (serum vitamin E levels, mg/100ml)=1.034-0.063x(month).

가축(家畜)의 장기(臟器)와 조직(組織)에 존재(存在)하는 조직비만세포(組織肥滿細胞)의 정상분포(正常分布)와 감염증시(感染症時)의 태도(態度)에 관한 연구(硏究)

김화식,이재현,Kim, Hwa Sik,Lee, Jae Hyun 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.2

The frequency distribution and morphological characteristics of tissue mast cells in the various tissues of normal domestic animals (bovine, swine, dog and chicken) and systematically infected (Hog cholera, Canine distemper and Newcastle disease) animals were studied. The results were as follows: 1 In cattle, density of mast cells was higher in foetus (bovine) and young animals than in adults Differences in frequency distribution among individual animals were also observed. 2. In chicken, the highest number of mast cells was found in age group of 15 to 40 days, the moderate number in age group of one to 10 days, and the lowest number in age group of 40 days or older. 3. The morphologically, mast cells were usually round, ovoid, spindle, oval and irregular, and particularly in ovary of bovine, it was usually round and ovoid forms. 4. The largest numbers of mast cells were shown in ovary of bovine, intestine of swine and dog, and proventriculum of chicken. 5. In the systemic infections, the number of mart cells usually tends to increase.

개의 Hemoglobin, Albumin 및 Transferrin의 형(型)에 관(關)한 연구(硏究)

김우권,Kim, Woo Kwon 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2

Hemoglobin, albumin and transferrin phenetypes observed in a population of 255 dogs of various breeds by agar gel electrophoresis for hemoglobin and albumin, and by starch gel electrophoresis, for transferrin. The results obtained were as follows: 1. The hemoglobin phenotypes were classified as HbAA, HbAB and HbBB and the frequencies of appearence were 91.77, 6.27 and 1.96%, respectively. Gene frequency studies of $Hb^A$ and $Hb^B$ showed the $Hb^A$ gene to have a frequency of 94.9% and $Hb^B$ gene to have a frequency of 5.1. The frequency of hemoglobin phenotypes of the Korean Jindo dog (ylelow) was HbAA 76.19%, HbAB 17.46% and HbBB 6.35% and HbBB was recognized only in the Korean Jindo dogs. 2. The albumin phenotypes were calssified as AlbAA, AlbAB and AlbBB and the frequencies of appearence were 28.71, 43.57 and 27.72% respectively. Gene frequency stduies of $Alb^A$ and $Alb^B$ showed the $Alb^A$ gene to hate a frequency of 50.50% and $Alb^B$ gene to have a frequency of 49.50. The frequency of albumin phenotypes of the Korean Jindo dog was 26.32% for AlbAA, 34.21% for AlbAB and 39.47% for AlbBB. The frequency of albumin phenotypes of the Korean Jindo dog was slightly different from others and gene frequency of $Alb^B$ was 56.58. 3. Transferrin studies resulted in findings of 10 different phenotypes and frequencies of transferrin phenotype were 30.08% for $TfA_1A_1$, 8.02 for $TfA_2A_2$, 2.11 for TfBB, 4.64 for $TfC_1C_1$, 1.69 for $TfA_1B$, 41.35 for $TfA_1C_1$, 5.49 for $TfA_2C_1$, 0.84 for $TfA_2C_2$ and 3.79 for $TfBC_1$. Gene frequency studies of $Tf^A$, $Tf^B$ and $Tf^C$ showed the gene to have a frequency of 63.08% for $Tf^A$, 4.85 for $Tf^B$ and 32.07 for $Tf^C$ 4. Transferrin studies revealed two different band patterns on starch gels with a strong and weaker stained one. The $TfC_2C_2$ and $TfA_2A_2$ which were found in the Korean Jindo dog only showed a weaker stained band and the gene frequency of $TfA_1A_1$ was highly recognized in this dog.

경주마(競走馬)의 혈액상(血液像)에 관(關)하여

김태종,이강욱,Kim, Tae Jong,Lee, Kang Wook 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.2

The blood picture of 85 healthy race horses in Korea was investigated. The ranges and mean values of erythrocyte, hemoglobin, hematocrit value, mean corpuscular volume, mean corpuscular hemoglobin concentration, and total white blood cell count in the blood picture were determine. The respective mean value and standard deviation and age differences were as follows: 1. The erythrobyte count was shown as range of 6.20 to $11.32{\times}10^6/mm^3$ with mean of $8.61{\pm}1.92{\times}10^6/mm^3$(SD). The leucocyte count was shown as range 5.0 to $18.0{\times}10^3/mm^3$ with mean of $8.25{\pm}1.51{\times}10^3/mm^3$(SD). There were not significant. differences in age, 2. The mean value of hemoglobin was shown $13.9{\pm}1.7g/100ml(SD)$ ranging 9.8 to 16.8g/100ml. The mean value of hematocrit was shown $40.9{\pm}3.94ml/100ml(SD)$ ranging 26 to 54. There were not significant differences in age. 3. The mean corpuscular hemoglobin was shown as range of 11.8 to 22.2pg with mean of $16.9{\pm}4.69$(SD). The mean corpuscular volume was shown as range of 34.5 to $71.3cu{\mu}$ with mean of $49.0{\pm}7.32cu{\mu}$(SD). The mean corpuscular hemoglobin concentration was shown as range of 30.6 to 39.4 g/100 ml with mean of $34.6{\pm}2.36$(SD). There were not significant differences in age. 4. The correlation among erythrocyte count, hemoglobin and hematocrit value were observed as follows: Erythrocyte count and hemoglobin (+0.328), rythrocyte count and hematocrit vague (+0.319). A linear regression equation was shown as follows: Erythrocyte count and hemoglobin (Y=0.336x+10.977), erythrocyte count and hematocrit value (Y=0.655x+35.274). 5. The high correlation between hemoglobin and hematocrit vague was observed (r= +0.836). A linear regression equation was shown: (Y=1.948x+13.895).