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Saccharomyces cerevisiae와 candida cariosilignicola사이의 세포융합에 관한 연구
이재동,임하선 한국미생물학회 1988 미생물학회지 Vol.26 No.1
This research was focused on investigation of the condition for protoplast formation and regeneration of protoplast fusion between Saccharomyces cerevisiae which has fermentation ability and Candida cariosilignicola which can grow at high temperature and utilize methanol. The results obtained were as follows; The highest production was collected in exponential growth phase. Ninety-nine% protoplast formation of C. cariosilignicola was obtained in glycin-NaOH buffer (pH10.0) containing Zymolyase 0.5mg/ml at $35^{\circ}C$ for 1hr incubation. The highest regeneration was produced when protoplast wuwpension containing 0.5% soft agar in buffered 50mM $CaCl_{2}$ was poured as a soft overlay onto 2% agar plates. Equal amuont of protoplast suspension of two strains was mixed and centrifuged. The subsequent pellet was added to 2ml of 35% polyethylene glycol (MW 4,000) containing 50mM $CaCl_{2}$, and incubated at $30^{\circ}C$ for 10min. Then 0.1ml of the suspension of aggregated protoplast was immediately covered with minimal medium and incubated at $40^{\circ}C$ for 5-7 days. As results, $SC_{1}$, $SC_{2}$, and $SC_{3}$ fusants were obtained. The physiological characteristics of fusants produced by protoplast fusion were; $SC_{1}$, and $SC_{2}$ utilized maltose, galactose, methanol, potassium nitrate. $SC_{3}$ utilized all the above materials except galactose.
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Candida Antarctica Lipase를 이용한 폐유의 바이오디젤의 제조
이재동 한국폐기물자원순환학회 2013 한국폐기물자원순환학회지 Vol.30 No.4
Enzymatic biodiesel using lipase Novozyme-435 instead of using chemical catalyst was studied in this paper for cost reduction, increase of production efficiency and environment friendly product in this paper. In this paper the standard of biodiesel quality was met by using enzyme. The optimal reaction condition of methanol/waste oil molar ratio was 3, which 1 mol of methanol was injected at initial reaction. Then, each of 1 mol of methanol was injected at 4 hours of interval. Other reaction condition was like as follows : Reaction temperature, reaction time, agitation speed and amount of lipase were 55oC, 24 hours, 400 rpm, and 6wt%, respectively.
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