정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.

수종의 밴드 접착 시멘트의 물성에 대한 비교 연구

양규호,김기백,김선미,최남기 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.3

본 연구의 목적은 시중에 유통되고 있는 밴드 시멘트들을 종류별로 이용하여 교정용 밴드의 결합강도를 비교하고, 각 시멘트의 파절 양상을 비교하여 교정용 밴드에 대한 사용지침을 마련하는데 도움이 되고자 하였다. 100개의 발거된 인간의 제 3대구치를 이용해 실험군은 총 5개의 군으로 하였으며, 각 군당 시편수가 20개가 되도록 임의적으로 분류하였다. 실험에 사용한 밴드 시멘트는 다음과 같다; Ormco gold, Ultra Band-Lo™, Fuji Ortho™ LC, 3M Unitek™ Multi-Cure Glass Ionomer, Ketac-Cem™. Universal testing machine(Instron Corp., Canton, MA, USA)를 사용하여 최대하중값을 측정하였고, 전단결합강도 값을 계산하였다. 밴드가 탈락한 후, 탈락부위를 평가하여 법랑질과 시멘트, 시멘트와 밴드 사이로 구분하였다. 밴드의 전단강도는 One-way ANOVA를 이용하여 통계처리 하였으며 Tukey test를 이용하여 검정하였다. 또한 탈락 부위는 Chi-squre analysis를 이용하여 통계 처리하였고, Fisher's exact test로 군간 유의성을 검정하였다. 실험 결과 평균 파절 강도는 Ormco군이 가장 높았고(2.44±0.57), Fuji Ortho™군(2.24±0.50), Ketac-Cem™군(2.10±0.57), 3M Unitek™군(1.82±0.43), Band-Lok™군(1.73±0.28) 순이었으며, Ormco군은 Band-Lok™군과 3M Unitek™군, Fuji Ortho™군은 Band Lok™군과만 통계적으로 유의할만한 차이를 보였다(p<0.05). 파절 양상에서 Ormco군과 Band-Lok™군은 서로뿐만 아니라 다른 군과 유의할만한 차이를 보였으며, Fuji Ortho™, 3M Unitek™, Ketac-Cem™군 간에는 유의한 차이가 없었다. The aim of this study was to compare the shear-peel strength and the fracture site of 5 commercially available orthodontic band cements. One hundred molar bands were cemented to extracted human 3rd molars. The specimens were prepared in accordance with the manufacturer's instructions for each cement. After storage in a humidor at 37℃ for 24 hours. the shear debonding force was assessed for each specimen using an universal testing machine with crosshead speed of 2 mm/minute. Maximal failure stress was converted to mean shear-peel strength. MPa. The predominant site of band failure was recorded visually for all specimens as either at the band/cement or cement/enamel interface. Mean shear-peel strength of Ormco was the highest(2.44±0.57). followed by Fuji Ortho™(2.24±0.50), Ketac-Cem™(2.10±0.57). 3M Unitek™(1.82±0.43). Band-Lok™(1.73±0.28). There were statistically significant differences between Ormco and Band-Lok™. Ormco and 3M Unitek™. and Fuji Ortho™ and Band-Lok™(p<0.05). The predominant site of bonding failure for bands cemented with the Ormco was at the band/cement interface. whereas bands cemented with Ultra Band-Lok™ failed predominantly at the enamel/cement interface. There was no significant difference among the other cements(Fuji Ortho™. 3M Unitek™. Ketac-Cem™).

師範系 體育敎育科 志望生의 一般運動能力檢査 基準値 設定에 關한 硏究

梁點紅,高基俊,黃喆文,金泰運,金重雄,白永鎬,表內淑,黃太相,徐國雄 釜山大學校 附設 體育科學硏究所 1989 體育科學硏究所 論文集 Vol.5 No.-

This study was analyzed the validity and the reliability of evaluation norm. which was used in choosing and grading events of the General Motor Ability Test. The General Motor Ability Test has been done for applicants for entrance to the department of physical education, college of education, Pusan National University. The subjects of this study were 433 applicants(men :387, women :46) for three years. They were tested in seven events : 1) 100m Dash, 2) 1500m Run(man), 800m Run (woman), 3) Throwing Hand-Ball, 4) Broad Jump, 5) Chinning(man), Flexed-Arm Hang(woman), 6) Zig-Zag Run, 7) Trunk Flexion. The results could be summerized as follows ; 1. In 100m Dash, 1500m Run, and Throwing Hand-Ball, man's average records were shown normal distribution which was inclined toward low ll grade. Therefore, the interval size of those three events must be regulated to lower. 2. Average records of Broad Jump in man were distributed into high (1) grade. So, the interval size must be adjusted to higher. 3. In Chinning, Zig-Zag Run, ad Trunk Flexion, man's average records were shawn normal distribution, and the interval size was similar to present records. 4. For woman, it was impossible to analyze correctly in all seven events because of the small number of subjects. So, it was for the sake of the reference. 5. The record of applicants for three years were operated to the variable quantity analysis. And the restults were these ; 100m Dash was F=3.0865(p<0.05), Chinning F=3.9309(p<0.05), Broad Jump F=14.7338(p<0.001), Throwing Hand-Ball F=4.9716(p<0.01), and Zig-Zag Run F=6.8297(p<0.001). Then, it was shown statistical meaningful difference in three groups. 6. In investigating the validity in choosing seven events, it was concluded that the events have been chosen reasonably. Because all events except for 100m Dash, and Zig-Zag Run in man were independent. From the analysis of the General Motor Ability Test, we could realized the necessity of regulating the standard and the interval size in several events. Then, we proposed the new evaluation norm which was suitable to present situation.

대조 추출물분획이 치은 섬유아세포의 생물학적 활성화에 미치는 영향

양창호,이용무,조기영,배기환,정종평 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

Final goal of periodontal treatment is to reconstruct the destructed periodontal tissue as well as to remove the necrotic pathologic elements. The purpose of this study is to investigate on the effect of Zizyphi extract to the inhibitory ability on collagenolytic activity of P gingivalis, biologic activity of gingival fibroblasts and on the collagen and protein synthesis of gingival fibroblasts. Gingival fibroblast from giniva of first bicuspids from patient for orthodontic treatment were used and cultured. For the measurement of inhibitory ability of collagenolytic activity, crude enzyme was extracted and used on the basis of modified Ono's method. On the inhibition of collagenolytic enzyme from herbal extracts, collagenokit CLN-100 were used. The cellular activity of gingival fibroblast, were studied using MTT solution and measured optical density on 570mm by ELISA reader. To measure the effects on the ability of whole protein and collagen synthesis, cell membrane was destructed with ultrasonic grinder after culturing, centrifuged and counted by liquid scintilation counter. The inhibitory effects on producing of IL-1β by monocyte, after promotion of producing IL-1β by LPS, were compared with the mixture of herbal extracts and other drugs using thymocyte stimulation assay. About inhibitory·effects of PGE_2 by gingival fibroblasts, herbal extract was compared with the addition of the other control groups using enzyme immunoassay. On the inhibition of collagenolytic activity by P. gingivalis, benzene extracts showed the most efficient inhibitory effects among the l9㎍/㎖ of the compared extracts and 40.5% by Tetracycline. On the cellular activity promoting effects, compared extracts showed a bit of more effects than PDGF of l00㎍/㎖ concentration and lGF of 20㎍/㎖ concentration. All of the PDGF, 1GF, Zizyphi Fructus extract should increase in collagen synthesis, but especially 70% ethylalcohol extracts of Zizyphi Fructus showed comparably high effects among the compared extracts. Effects on whole protein synthesis were slightly increased on every extract but especially 70% ethylalcohol extract showed significantly effective than any other estract. On the inhibitory effects of Zizyphi Fructus IL-1β production by monocyte, compared extracts showed 70% of highly inhibitory effect than that of 60% inhibition effects on controlled group and each extracts showed no significant difference. In PGE_2 production inhibitroy effect of Zizyphi Fructus gingival fibroblasts, Herbal extracts showed 70% of inhibition comparing with tat of 90.2% of controlled group, but each extracts showed similar effects excluding the H_2O extracts. These results suggested that Zizyphi Fructus might be useful medicine for inhibition of inflammatory mediator including IL-1β and PGE_2·

이상와 누공(piriform sinus fistula)에 기인한 재발성 화농성 갑상선염 1예 : A Route of Infection in Recurrent Suppurative Thyroiditis

양근호,김홍주,김기환 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Piriform sinus fistula, believed as an embryonic remnant of the third or forth pharyngeal pouch, is an extremely rare and newly recognized entity that lead to acute suppurative cervical infection. Recently, reports of acute suppurative thyroiditis and anterior neck abscess due to piriform sinus fistula have been increasing in number, though an extensive review of the literature reveals that about 60 cases of this condition have been reported. The sinus may be overlooked for years and the patient may undergo multiple procedures before the diagnosis is properly made and treated. We have experienced such a case of recurrent suppuative thyroidits in which coexisting piriform sinus fistula was identified by intra-operative endoscopic examinaton. This report reviews the embryological development of the sinus and the clinical symptoms and treatment of this condition.

과학 영재 프로그램을 통해 본 과학 관련 태도와 과학 불안도의 관계와 변화

양태연,박상우,박인호,한기순 한국과학교육학회 2005 한국과학교육학회지 Vol.25 No.2

본 연구는 과학 영재 학생의 과학 관련 태도와 과학 불안도와의 관계가 어떠한 상관을 보이고 있고, 과학 관련 태도와 과학 불안도가 과학 영재 프로그램에 참여하기 전과 참여한 후에 어떻게 변화하는지를 보았다. 이에 따라 과학 영재 교육 프로그램에 참여하고 있는 148명의 중학생들을(남학생 : 95명, 여학생 53명)대상으로 과학 관련 태도 검사와 과학 불안도 검사를 실시하였다. 연구결과, 과학 관련 태도와 과학 불안도의 관계는 사전 검사에서 뚜렷한 부적 상관을 보였으나 사후 검사에서는 부분적으로 정적 상관이 나타났고 영재 프로그램 참여 후 과학 영재들의 과학 관련 태도는 낮아졌고 과학 불안도는 증가하였다. 이 연구의 흥미로운 점은, 여러 선행 연구결과와는 달리 영재 프로그램에 참여한 후 남학생이 여학생보다 과학 관련 태도가 낮아졌고 과학 불안도가 증가하였다는 점이다. This study examined scientifically gifted students' science attitude, anxiety, and their relationship. In addition, sex differences in these variables were investigated. The main interest of the study, however, was to explore how scientifically gifted students' science attitude and anxiety have changed before and after a gifted education experience. One hundred and forty-eight middle school students (95 boys and 53 girls) who enrolled in a gifted education program participated in the present study. The results indicate an increase in science anxiety and a decrease in science attitude after the six-month gifted program experience. Negative correlations between science attitude and anxiety were found in pre-test, but some positive relationship existed in post-test. Interestingly, after the specialized gifted program, male students' science attitudes were lowered significantly. In contrast, there was no statistically significant difference in female students. Results of the study were discussed in relation to the programming issues for the scientifically gifted.

Hippocampal CA 2-3 부위의 Ubiquitin 양성 Neuritic Thread와 대뇌피질 Lewy Body와의 관계

양기화,양기승,강충구,성주호 대한병리학회 1995 Journal of Pathology and Translational Medicine Vol.29 No.5

급성충소돌기염의 임상 : 병리학적 고찰

양기화,조준필,하영천,한진우,정학재,손수창,이성희,황재훈,신철호 최신의학사 1989 最新醫學 Vol.32 No.4

제주 곽지유적에서 출토된 말 뼈의 골학적 관찰

양기천,신태균,김승호 제주대학교 1993 논문집 Vol.37 No.-

Equine bones including one phalanx 3 and three teeth excavated from Kwakji archaeological site, were morphologically examined, Compared with the Cheju pony and the Thoroughbred horse, the significance of the observations were tentatively evaluated. It was suggestied that the larger-limbed horses than the present Cheju pony inhabited in ancient Cheju island.