약학대학에서 문제중심학습법의 효과 분석 : 충남대학교 약학대학 사례를 중심으로

윤휘열,김정태,유영훈,백현문,송병정,권광일 충남대학교 약학대학 의약품개발연구소 2016 藥學論文集 Vol.31 No.-

After exchanging pharmacy edcuation system from 4th years to 6th years at 2011, Problem Based Learning (PBL), expecially fields of pharmacotherapy, is considered as alternative teaching implements to enhance student’s skills about solving-problem, critical thinking, clinical reasoning and self-motivated learning. The purpose of this study is to analyze the effectiveness about PBL class in clinical pharmacy students, and provide the evidence of its application. Four groups consisted of 10~12 students on each (Total number of students were 54) had PBL class with 4 pharmacists who were pre-educated about PBL class as tutors. After PBL class, all of students took part in the paper-based survey about effect of PBL class in clinical pharmacy education. In results of survey, the effectiveness of PBL was superior in comparisons with traditional teaching method and student’s attitudes for class such as satisfaction and participation were also improvements. In conclusions, PBL methods had a lots of benefits about improvement of academic performance and professional knowledges in clinical pharmacy teaching, therefore PBL class should be suggested more frequently in clinical pharmacy.

닭의장풀의 Iridoid, Triterpenoid 및 Steroid 성분에 관한 연구(Ⅱ)

백수현,서원준,배기환,진갑덕 충남대학교 약학대학 의약품개발연구소 1990 藥學論文集 Vol.6 No.-

Three non-alkaloidal constituents, (-)-loliolide as an iridoid, friedelin as a triterpenoid, and β-sitosterol as a steroid, were isolated by fractional extraction and silica gel column chromatography from the herb of Commelina communis L. (Commelinaceae). The structures of the native components were elucidated on the basis of physical and spectral (UV, IR, PMR, CMR, and mass spectra) data.

크로마토그래프법을 이용한 사삼(沙蔘) 및 유사생약의 패턴분석 비교 연구

강종성,김경태,이은실,이영근,이영종,황귀서 충남대학교 약학대학 의약품개발연구소 2013 藥學論文集 Vol.28 No.-

Adenophora Radix is the dried root of Adenophora triphylla var. japonica Hara or the other crude drugs belong to genus Adenophora. However, other crude drug like Codonopsis lanceolata or C. pilosula were often misused as Adenophora radix. A pattern analysis method to evaluate the Adenophora Radix and similar crude drugs were carried out with the methanol and ethylacetate extract by HPLC and TLC. The components were separated by HPLC on ODS column with gradient elution of solvent A (10% acetonitrile) and B (70% acetonitrile). The multivariate peak data of the chromatograms of extracts of samples were used for hierarchical clustering analysis, principal components analysis and similarity calculation. The similarities like Euclidean distance, cosine and correlation for the methanol extract of A. tetraphylla and A. remitiflora showed good correlation with A. triphylla var. japonica, indicating that the similarity could be used as a chemical taxonomy. The genus Adenophora and genus Codonopsis were well classified by the hierarchical clustering method for the cluster analysis. The developed pattern analysis method was specific and could be readily utilized for comprehensive evaluation of Adenophora Radix.

GPR41 효능제의 3T3-L1 지방세포 지질축적 억제효과

이미지, 이도형, 조준환, 한주희, 명창선 충남대학교 약학대학 의약품개발연구소 2021 藥學論文集 Vol.36 No.-

G protein-coupled receptor 41 (GPR41) is activated by short chain fatty acids (SCFAs) such as acetate (C2), propionate (C3), butyrate (C4). It is known to stimulate the secretion of glucagon-like peptide-1(GLP-1) in the intestinal enteroendocrine L-cell and leptin in adipocytes. The aim of this study was to investigate the inhibitory effect of GPR41 agonists including AR420626, 1-methyl cyclo- propane-1-carboxylic acid (1-MCPC) and propionate on lipid accumulation in 3T3-L1 adipocytes. GPR41 agonists did not show cytotoxic effect at all concentration tested. Each GPR41 agonist reduced lipid ac- cumulation in 3T3-L1 adipocytes. They decreased the expression of peroxisome proliferator-activated re- ceptor-g (PPAR-g) that contributes to the regulation of lipid accumulation. Among three agonists, AR420626 showed the greater effect on the regulation of lipid accumulation than 1-MCPC and propionate. Therefore, this observation indicates the beneficial effect of GPR41 activation for the man- agement of obesity.

(+)-Chromen 유도체의 합성 및 이들의 Wnt/β-catenin pathway 억제 활성 평가

이지현,조수현,윤은주,오유석,송규용 충남대학교 약학대학 의약품개발연구소 2013 藥學論文集 Vol.28 No.-

(+)-Decursin and GCK062, which is decursin derivative, showed strong inhibitory activity for Wnt/β-catenin signaling and suppressed concentration-dependently PC3 cell growth. These compounds have pyranocoumarin ring as backbone and acryloyl group as side chain. In this study, we introduced (+)-chromen ring as backbond, that is more simplified than pyranocoumarin, and synthesized (+)-chromen derivatives. As a results, in most cases, (+)-chromen derivatives disappeared or lowered the inhibitory activities for Wnt/β -catenin signaling. However (+)-chromen derivative (SLC-G005, 92.0%), introduced 3,4-dihydroxycinnamoyl group, showed potent inhibitory activity, not as much as GCK062 (99.5%).

혈소판 응집 억제제의 효과 측정법에 대한 고찰과 지원자에서 Triflusal의 혈소판 응집 억제능 평가

이병요,장힘찬,백인환,윤휘열,권광일 충남대학교 약학대학 의약품개발연구소 2010 藥學論文集 Vol.25 No.-

The anti-platelet agent is a member of a class of pharmaceuticals that decreases platelet aggregation and inhibits thrombus formation. They are effective in the arterial circulation and widely used in primary and secondary prevention of thrombotic cerebrovascular or cardiovascular disease. As a method for estimating the effects of anti-platelet agent, platelet aggregation was conventionally measured using the optical method or the impedance method. Several alternate methods currently in development or recently developed were considered, including luminescence method, flow cytometry, laser-light scattering method, and Verify Now-P2Y12 assay. Principles, advantages, and disadvantages of the optical method, impedance method, and the other alternate platelet aggregation methods were discussed in this report. 15 human volunteers were recruited for the evaluation of the efficacy of triflusal using the optical method. After the oral administration of a single dose of 900mg, 15 subjects received eight doses administered at 24-hour intervals of 600mg triflusal. Using platelet rich-plasma from above subjects, we performed baseline platelet aggregation test induced by adenosine diphosphate(ADP), collagen, and arachidonic acid. The results of platelet aggregation test after triflusal administration were compared with the baseline study. Triflusal significantly inhibited platelet aggregation induced by ADP (33.0±21.3%) and arachidonic acid (99.1±1.2%), respectively. Therefore, we concluded that anti-platelet aggregation effect of triflusal can be studied successfully with the optical method. Each of the platelet aggregation methods has value for evaluating the effects by various mechanisms of the anti-platelet agents. The ideal method for estimating the platelet aggregation as it relates to safety and efficacy in patients treated with anti-platelet agents will need to be determined in clinical trials.

베타차단제와 베타-사이클로덱스트린 복합체 내에서의 광학선택성에 대한 분자역학적 연구

장석영,박경래 충남대학교 약학대학 의약품개발연구소 2003 藥學論文集 Vol.18 No.-

Beta-blockers have been used in the treatment of hypertension, angina and cardiovascular disorder Their pharmacodynamic and pharmacokinetic characteristics between (R) and (S) enantiomers are different. We selected β-cyclodextrin (BCD) as a chiral selector and metoprolol (MT) and propranolol (PNP) as quest molecules to investigate mechanism for separation. The binding interactions and energetics in inclusion complex of (S)- and (R)-enatniomer in BCD were investigated by molecular dynamics (MD) simulation method. In the inclusion complex the guest molecules restrains the host molecule to a smaller range of atomic fluctuation due to the electrostatic interactions between polar groups of guest and BCD Free energy calculation for the transition of (S)- to (R)-form was performed using slow-growth method. The improper dihedral angle around the chiral center of (S)-form was perturbed to (R) form The thermodynamic integration gave a free energy change from (S)-MT-BCD to (R)-MT-BCD of 0.98 kJ/mol, which predicts the bigger stability of (R)-MT-BCD complex than that of (S)-isomer. In the case of PNP-BCD complex, a free energy change of 0.95 kJ/mol indicates that (S)-form complex was more stable than (R)-form complex.

황련해독탕 및 귀비탕의 발효 전후 혈소판 응집 억제 효과에 대한 비교 연구

송병정,이수진,권광일 충남대학교 약학대학 의약품개발연구소 2012 藥學論文集 Vol.27 No.-

Recently interest of fermented oriental medicines is increasing for the purpose of disease prevention. Hwangryunghaedok-tang (HR) and Guibi-tang (GB) are widely used oriental medicines in Korea. HR and GB are well known for anti-inflammatory and anti-stress effect. Glycosides in herbal medicines are transformed to aglycone via fermentation. The fermented medicines are absorbed more and have less individual variation. In this study, HR and GB were compared before and after fermentation by anti-platelet aggregation effect. HR and GB were fermented by various lactobacillus species. The anti-platelet aggregation effect of HR and GB were measured using aggregometer by optical method. Collagen (4 μg/mL) was used as a inducer of platelet aggregation. After measuring anti-platelet aggregation effect, we analysed the data using WinnonlinⓇ program to fit simple Emax model. Then Emax and EC50 values were estimated. In results, GB had little antiplatelet effects. Emax of 5 GB samples (127, 164, 402, 442, 3163) were estimated near zero and others (129, 144, 166, 693, 744) had high EC50 values (> 1500 μg/mL). In contrast with GB, HR had antiplatelet effects. HR which was fermented by Lactobacillus casei and Lactobacillus plantarum (HR-127,HR-144) were most effective sample. Emax and EC50 of HR-127 and HR-144 was 99.99%, 174.73 μg/mL and 100.00% 160.48 μg/mL, respectively. These results indicated that Guibi-tang was inappropriate as anti-platelet aggregation agent and Hwangryunhaedok-tang would be more effective after fermentation as anti-platelet aggregation agent, warranting further study.

2-Propylthio-5,8-dimethoxy-1,4-naphthoquinone의 항암활성에 관한 연구

송규용 충남대학교 약학대학 의약품개발연구소 2004 藥學論文集 Vol.19 No.-

In a continuation of the search for novel antitumor agent, 5,8-dimethoxy-1,4-naphthoquinone was selected as a lead compound and 2-alkylthio-5,8-dimethoxy-1,4-naphthoquinone derivatives were synthesized from 1,5-dihydroxynaphthalene in 5 steps. Among them, 2-propylthio-5,8-dimethoxy-1,4-naphthoquinone (2-PropylthioDMNQ) exhibited the strongest cytotoxicity (ED_(50), 2.02±0.89 and 9.44±199 μM) against L1210 and A549 cells, respectively. Also, 2-propylthioDMNQ (6C) strongly inhibited adhesion of B16-BL6 cells to gelatin and matrigel coated matrices and exhibited anti-invasive effect of B16-BL6 cells. The T/C value of 2-propylthioDMNQ (6c) treated group was 123±2.65 % in S-180 bearing ICR mice.

A Consideration on Lipid-based Nanocarriers of Peptide/protein for Topical Delivery

Hye-Jin Lee, Eun Joo Lee, Cheong-Weon Cho 충남대학교 약학대학 의약품개발연구소 2021 藥學論文集 Vol.36 No.-

From a biopharmaceutical point of view, the skin is recognized as an interesting route for drug delivery. Typically, small molecules can penetrate the outermost layer of the skin, the stratum corneum. In contrast, delivery of larger molecules such as peptides and proteins is still challenging. Nanoparticles can be used not only to enhance the penetration of drugs into the skin, but also to expand the range of molecules used clinically. This review focuses on solid lipid nanoparticles, nanostructured lipid carriers and lipid polymer nano-particles for topical administration. They are composed of lipids similar to those of the skin and sebum, which have limited toxicological risks and contribute to improved biocompatibility. Therefore, this review aims to sum- mary the lipid-based nanostructures designed for the topical administration of peptides and proteins including their general evaluation methods.