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김기환,김태연 한국방위산업학회 2023 한국방위산업학회지 Vol.30 No.1
This study identifies problems with the Offset quantitative performance management methodology proposed by Kim Ki-hwan and Lee Sang-bok (2022) in 2022 to quantitatively evaluate the performance of R&D technologies obtained through Offset. In addition, domestic defense and private sector rating methodology management institutions are selected as advisory institutions to verify the validity and fair evaluation of major technical achievements obtained through compromise offset, and the characteristics and advantages of each institution are analyzed. Finally, to systematically database and manage the results created, a ‘computer management system construction plan’ is proposed by deriving essential elements of the computer management system and analyzing the system needs. 본 연구에서는 절충교역을 통해 획득된 연구개발 기술 등의 이행성과를 정량적으로 평가할 수 있도록 2022년에 김기환・ 이상복(2022)이 제안한 절충교역 정량적 성과관리 방법론에 대한 문제점을 식별하여 보완방안을 제안한다. 또한, 절충교역으로 획득된 주요 기술성과들의 타당성 검증 및 공정한 평가를 위하여 국내 국방 및 민간분야 등급평가 방법론 운용기관들을자문기관으로 선정 후 각 기관의 특성과 장단점을 분석하여 ‘절충교역 성과평가위원회 운영방안’을 제안한다. 마지막으로 창출된 성과를 체계적으로 DB화하여 관리할 수 있도록 전산관리 시스템 필수요소 도출 및 시스템 요구도를 분석하여 ‘전산관리시스템 구축방안’을 제안한다.
가뭄 스트레스 특이적인 cis-regulatory element의 특성을 기반으로 한 신규 프로모터 구축
김기환,김병규,신주형,김원찬 한국응용생명화학회 2021 Journal of Applied Biological Chemistry Vol.64 No.1
Droughts are one of the abiotic stresses that hinders the growth and productivity of crop plants. Coping with abiotic stress is necessary to understand the molecular regulatory networks that makes plants respond to adverse environmental conditions. In our experiment to find a combination that can cope with abiotic stress (respond to drought), we screened 5 stressinducible promoters that are expressed only under stress conditions. This founded 36 cis-elements in stress-inducible promoters. With the result we designed 2 synthetic promoters (BL1, BL2) for fine-controlled regulation by assembling ciselements from the native promoters, which are expressed only under stress caused by droughts. Analysis of the transgenic plant (BL1-GUS, BL2-GUS) showed that the synthetic promoters increased the expression of β-glucuronidase (GUS) in transgenic plants under desiccation. Also in the transient activation assay demonstrated that synthetic promoters induced the cotransformation of effector DREB1A and DREB2C. These results expect that the synthetic promoter with a combination of droughtspecific elements can be used to respond to various abiotic stress and is resistant to stress without causing growth retardation.