Ho-166 부착풍선도자를 이용한 방사선 조사의 돼지 관상동맥 스텐트 재협착 예방 효과

김원 ( Kim Won ),정명호 ( Jeong Myeong Ho ),박옥영 ( Park Og Yeong ),정우곤 ( Jeong U Gon ),박우석 ( Park U Seog ),김주한 ( Kim Ju Han ),안영근 ( An Yeong Geun ),조정관 ( Jo Jeong Gwan ),박종춘 ( Park Jong Chun ),강정채 ( Kang Je 한국지질동맥경화학회 ( 구 한국지질학회 ) 2002 韓國脂質學會誌 Vol.12 No.1

배경 : 국내에서 개발된 방사선 동위원소 Holmium-166 (166Ho)은 주로 베타선을 방출하며, 166Ho을 부착한 풍선도자를 이용하여 돼지 관상동맥 재협착 모형에서 풍선확장술 후 신생내막 증식을 전신적 부작용 없이 안전하고 효과적으로 억제하였음을 보고한 바 있다. 본 연구에서는 돼지 관상동맥 스텐트 재협착 모형에서 스텐트 시술 후 신생내막 증식에 의한 재협착 병변을 166Ho 부착 풍선도자를 이용하여 치료하여 그 효과를 관찰하고자 하였다. 방법

In vitro and in vivo anti-inflammatory effects of pegmatite

Jo, Wol-Soon,Yang, Kwang-Mo,Choi, Yoo-Jin,Jeong, Chang-Hwa,Ahn, Kyoung-Jin,Nam, Byung-Hyouk,Lee, Sang-Wha,Seo, Su-Yeong,Jeong, Min-Ho The Korean Society of Toxicogenomics and Toxicopro 2010 Molecular & cellular toxicology Vol.6 No.2

Pegmatite is a coarse-grained intrusive igneous rock rich in rare elements such as uranium, tungsten, and tantalum with Ca, K, Mg, Fe, Se, Ge, and Ho. We tested in vitro and in vivo assays for the anti-inflammatory activity of pegmatites. We firstly evaluated the suppressive effects of pegmatite on macrophage cell line RAW 264.7 cells stimulated with proinflammatory stimuli lipopolysaccharide (LPS) to determine nitric oxide (NO) production and TNF-$\alpha$ and IL-6 release. The $IC_{50}$ values of pegmatite exceeded $5,000\;{\mu}g/mL$. Treatment of RAW 264.7 cells with pegmatite significantly inhibited LPS-stimulated NO production and proinflammatory cytokines such as TNF-$\alpha$ and IL-6 secretion in a dose-dependent manner (P<0.05). In vivo studies were tested with two animal models of arachidonic acid-induced mouse ear edema and an acetic acid-induced increase in capillary permeability. The pegmatite significantly attenuated ear edema induced by arachidonic acid and reduced the acetic acid-induced increase in capillary permeability in mice (P<0.05) when the pegmatite was administered topically (10 mg per ear) for 24 h. Therefore, pegmatite potentially shows an anti-inflammatory activity in the in vitro and in vivo mice and in the development of newer anti-inflammatory agents as mineral materials.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Vitamin D_(3)가 RAW 세포에 감염된 Mycobacterium marinum의 증식억제에 미치는 기전 연구

박정규,정샛별,이길수,김수영,송창화,박종호,조현구,조은경,김화중 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

The natural resistance-associated macrophage protein 1(Nramp1) has been proposed to directly regulate bactericidal or bacteriostatic activity of the macrophage toward pathogens or participate in macrophage activation that lead to microbial elimination in the host. The relationship between Nramp1 and nitric oxide(NO) as an antimicrobial factor has not been precisely defined to date. To devise an in vitro assay for Nramp1 function, this study introduced a wild type Nramp1^(G169) cDKA transfected RAW264.7 macrophages(A8) which bear a homozygous mutant Nramp1^(D169) allele and are permissive to replication of specific intracellular parasites. RAW264.7 and A8 macrophages did not produce NO, but vitamin D_(3)-activated-Mycobacterium marinum-infected RAW264.7 and A8 macrophages pretreated with vitamin D_(3) leaded to the increase of NO production and growth inhibition of M. marinum. Inhibition of NO production by a NO inhibitor, L-NAME, abolished the above effects. The mRNA expression of iNOS in infected macrophages with costimulated vitamin D_(3) was increased. IFN-γ activated macrophages also showed the same results with vitamin D_(3) activated macrophages. These results suggest that bactericidal or bacteriostatic activity in RAW264.7 and A8 macrophages correlated with the production of NO, although NO might not be the only factor responsible for controlling M. marinum infection. The Nrampl gene is considered to be a cofactor in the controlling the replication of M. marinum infection.

족관절 거골 골내결절종 : 2례 보고 2 cases report

선호영,조성일,이정웅,진병록,정산돈 최신의학사 1993 最新醫學 Vol.36 No.6

Chitosan을 이용한 초지공장의 폐수처리

김정호,신영조,유재홍 釜山大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.51 No.-

파지를 이용하여 판지·골판지 등을 제조하는 공장의 폐수는 대부분 콜로이드상으로 분산되어 있고 그 양이 대단히 많아 수질오염의 원인이 되므로 화학적 처리에 의한 현탁입자의 제거가 요구된다. 오징어의 뼈로부터 얻은 Chitosan(β-D-Glucosamine)을 이용하여 초지공장폐수의 응집효과를 실험하였다. 정제·건조된 Chitosan 0.5g을 0.5%의 CH₃COOH수용액에 녹여 응집제로 사용하였다. 응집시험은 Jar Test에 의해 최적 응집조건(온도, pH, 응집제의 양)을 결정했다. 최적조건을 결정하는 데 탁도, SS, 침강시간, COD, 탈수시간, Floc의 크기를 관찰하였다. 응집시헙은 22℃∼25℃에서 행하였으며 시험결과 Chitosan은 pH가 6일 때 탁도, SS, 침강시간, 탈수시간, Floc의 크기가 양호했으며, Chitosan의 양이 30ppm일 때 가장 적합했다. Chitosan과 Acrylamide계 응집제를 pH 6에서 공용했을 때는 응집제의 양이 40ppm에서 적합했다. 천연고분자인 Chitosan은 기존의 Acrylamide계 응집제와 응집비교한 결과 응집효율이 좋았으며, 무독, 무해하여 수질 환경개선에 기대된다. The wastewater from manufacturing industry which produce paperboards by using remnants of paper is discharged in a great quantity, in the dispersal colloidal state, and required chemical treatment to remove suspended solids which can cause water contamination. Flocculation experiments were done by using chitosan(β-D-Glucosamine) obtained from bones of squids. Refined and dried chitosan of 0.5g was dissolved in 0.5%CH₃COOH and used as a flocculant. Optimum condition of flocculation(temperature, pH, content of flocculant) was determined through jar test. Turbidity, content of SS(suspended solids), settling time. COD, dewatering time, and size of floc were investigated to find optimum condition. Experimental temperature was 22℃∼25℃. As a result turbidity, content of SS, settling time, size of floc, and dewatering time were improved in the process of flocculation when pH=6. It showed optimum dosage when the content of chitosan was 30ppm. When chitosan and acrylamide were used together as a flocculant. it showed optimun condition when the content of flocculant was 40ppm at pH 6. Natural polymer, chitosan was more effective than acrylamide in the process of flocculation, because it is nonpoisonous and harmless, it is expected to improve water pollution.

소비자 문제에 대한 대응행동 : 여자대학생을 중심으로

임정빈,이영호,조미환,강은주 漢陽大學校 韓國生活科學硏究所 2000 韓國 生活 科學 硏究 Vol.- No.18

The purpose of this study was to provide with the basic research to aid to establish in the direction of consumer education, which investigate the actual condition of consumer complaining behavior according to consumer problems. Content analysis method was used and the sample 624 case was taken from report about experienced according to consumer problems by university of women. The major results are as follows: They have much more consumer complaining behavior, when they experience the problem about non-durable goods, and less experience them in case about durable goods. But they need to knowledge(information) about the durable goods and a way of settlement when they experience problems after service and structural problems.

허혈성 심질환에서 염증지표에 관한 연구

번정득,권영주,박상호,한대희,이상철,강진환,김명구,심규혁,최병조,온영근,현민수,김성구 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background : Atherosclerosis is the chief underlying cause of ischemic heart disease and there is increasing evidence that inflammation is an important determinant of the development of atherosclerosis. We assessed the levels of inflammatory markers in patients with ischemic heart disease and normal group who has normal coronary angiograms. Materials and Methods : Coronary angiography was performed in 142 patients. 107 patients of ischemic heart disease(stable angina pectoris 58, unstable angina pectoris 30, Acute myocardial infarction 19) and 38 normal control subjects. We assessed the level of inflammatory markers, such as CRP, ESR, fibrinogen and leukocyte. Results : CRP, ESR and fibrinogen values of the patients with stable angina pectoris and unstable angina pectoris were higher than that of normal control group, but there were no statistical significance. Leukocyte value of the patients with unstable angina pectoris(9003.3±701.5/mm^(3)) was significantly higher than that of the patients with stable angina pectoris(6685.5±245.8/mm^(3)) and normal control subjects(6394.3±235.1/mm^(3)). CRP, ESR and fibrinogen values of the patients with acute myocardial infarction were also higher than that of normal control subjects. CRP was 3.88±2.05 mg/dL in acute mocardial infarction group, and 0.29±0.15 mg/dL in normal control subject group(p<0.05). Fibrinogen was 541.6±45.1 mg/dL in acute myocardial infarction group, 321.4±25.6 mg/dL in normal control subject group(p<0.05). Leukocyte was 10942.1±737.6/mm^(3) in acute myocardial infarction group, 6394.3±235.1/mm^(3) in normal control subject group(p<0.05). Conclusions : This study demonstrate that CRP, fibrinogen and leukocyte values of acute myocardial infarction group were significantly higher than that of control group and stable, unstable argina pectoris group. Leucokyte values were significantly elevated in unstable angina group, but CRP values were not in unstable angina group.

BLDC 모터를 이용한 PV 양수 펌프 시스템

최성호,조정민,전기영,김성남,성낙규,김용주,한경희 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

In this paper, we adapted BLDC motor to PV water pumping systems to maintain high efficiency in the wide speed area. Also to design confidence, we adapted the vector control that drive the maximum torque at each speed limit. We designed optimal gain value of current, speed and pressure PI controller. Inverter gate pulse used Space Vector PWM to reduce torque pulsation of BLDC motor. Finally it was improve general matters of high water storage tank method by direct water supply pumping method.

림프구에 대한 clozapine의 생체내 유전독성

임정호,곽경필,김문두,강병조 대한생물치료정신의학회 1997 생물치료정신의학 Vol.3 No.1

최근 난치성 정신분열증 환자에서 가장 효과적인 약으로 알려진 clozapine은 골수에 작용하여 심각한 부작용인 무과립 구증을 일으킬 수 있다. 무과립구증의 원인 및 기전에 대한 연구는 매우 많으나 현재까지 확실한 결론이 나지 않은 상태이다. 저자들은 clozapine의 이러한 골수 독성에 근거하여 clozapine이 인체내에서 염색체에 어떤 변화를 주는지 아닌지를 알아보기 위해 clozapine 단독치료를 시행한 환자에게서 치료전과 치료중에 얻어진 말초혈액 림프구를 배양하여 염색체에 어떤 변화가 생기는지를 조사하였으나, clozapine에 의한 염색체의 자발적인 변이는 관찰되지 않았다. Objective : Many drugs and environmental chemicals can induce aneuploidy or chromosome instability in vivo or in vitro. Modification of the fidelity of cellular division may occur via a variety of mechanisms and chemical interactions. The clozapine is new antipsychotic drug with superior effect than classic antipsychotics. But, it may produce agranulocytosis and seizure in some patients. Agranulocytosis is a serious side effect of this drug but the cause and mechanism of this side effect is not clearly explained. Some study suggested that oxidative stress can induce chromosome aberration and the other study suggested that clozapine produce free radical and it can induce oxidative stress on some kind of cells. Therefore we decided to investigate whether clozapine and its metabolites can induce genotoxic effect in vivo. Method : The patients were chronic schizophrenic patients without a treatment at least one year. Diagnosis was made by DSM-IV criteria and agreed by both of two psychiatrists. Blood sampling was done before the treatment and during the treatment with considerable time interval. We used peripheral blood lymphocyte culture method and we counted the numerical aberrations, sister chromatid exchanges. Results : Chromosome aberration was not detected in all samples. Conclusion : Clozapine did not induce spontaneous chromosome aberration to human lymphocytes in vivo.