Anti-Inflammatory and Cytoprotective Effects of TMC-256C1 from Marine-Derived Fungus <i>Aspergillus</i> sp. SF-6354 via up-Regulation of Heme Oxygenase-1 in Murine Hippocampal and Microglial Cell Lines

Kim, Dong-Cheol,Cho, Kwang-Ho,Ko, Wonmin,Yoon, Chi-Su,Sohn, Jae Hak,Yim, Joung Han,Kim, Youn-Chul,Oh, Hyuncheol MDPI AG 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.4

<P>In the course of searching for bioactive secondary metabolites from marine fungi, TMC-256C1 was isolated from an ethyl acetate extract of the marine-derived fungus <I>Aspergillus</I> sp. SF6354. TMC-256C1 displayed anti-neuroinflammatory effect in BV2 microglial cells induced by lipopolysaccharides (LPS) as well as neuroprotective effect against glutamate-stimulated neurotoxicity in mouse hippocampal HT22 cells. TMC-256C1 was shown to develop a cellular resistance to oxidative damage caused by glutamate-induced cytotoxicity and reactive oxygen species (ROS) generation in HT22 cells, and suppress the inflammation process in LPS-stimulated BV2 cells. Furthermore, the neuroprotective and anti-neuroinflammatory activities of TMC-256C1 were associated with upregulated expression of heme oxygenase (HO)-1 and nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) in HT22 and BV2 cells. We also found that TMC-256C1 activated p38 mitogen-activated protein kinases (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathways in HT22 and BV2 cells. These results demonstrated that TMC-256C1 activates HO-1 protein expression, probably by increasing nuclear Nrf2 levels via the activation of the p38 MAPK and PI3K/Akt pathways.</P>

Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

혈액투석 환자의 가성 고칼륨혈증

최창렬,조형도,강태영,이준수,한호,정청일,이동규,유준호,한상웅,김호중 대한신장학회 2001 대한신장학회잡지 Vol.20 No.5

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

AUTO CAD를 이용한 NC선반작업의 공구경로 검증을 위한 교육용 프로그램 개발

한영호,김동직 建國大學校 附設 産業技術硏究所 1993 논문집 Vol.18 No.-

Using AUTO LISP, an educational program for checking tool paths for NC turning operation has been developed under the widely used AUTO CAD environment. This program enables the NC class to give students chances for visual experience for tool path checking on a desk-top PC although the class can not afford to provide the highly expensive NC machines. The program is equipped with the ability to convert the CAD data to NC data even though it is not fully developed at the present state. A general purpose tool path checking program is expected possible for other machining jobs than the turning operation on the same basis of this study.

간질환자의 간질 관련 외상

오동호,박운규,이영주,김희태,김승현,김주한,김명호,신동진 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

Fe-(6∼12wt. %)Al 압분체의 소결거동 및 고온내산화성

한정호,이동희 연세대학교 대학원 1981 延世論叢 Vol.18 No.1

본 연구는 고온내산화 재료인 Fe-Al계합금을 분말야금법으로 개발하기 위한 기초연구로서 Al함량이 비교적 높은 Fe-(6∼12wt.%)Al 압분체의 소결거동과 이들의 고온에서의 산화거동을 조사하는데 그 목적이 있다. 본 연구에서의 압분체는 순천분말과 50%Fe-50%Al 합금분말을 혼합하여 Al의 최종 조성이 6, 8, 10 및 12wt.%가 되게끔 하여 성형시킨 것이며, 이것을 여러가지 조건(소결온도 : 1,100℃, 1,200℃, 1,300℃ :소결시간 : 10분∼300분)하에서 소결하여, 각 단계에서의 밀도, 기공도, 치밀화변수의 측정 및 미세조직을 관찰하여 소결거동을 조사하였다. 또한 이들 소결압분체를 공기중, 900℃, 1,000℃ 및 1,100℃의 고온에서 최단 1분으로 부터 최장 15시간에 걸쳐서 산화처리를 험하여 이에 따른 중량증가의 측정과 미세조직 관찰을 병행하여 산화거동을 분석하였다. 본 연구의 결과에 의하면, 1,100℃의 고상소결에서는 합금화에 기인한 계속적인 팽창으로 말미암아치밀화가 이루어지지 않음을 알았다. 반면 액상소결의 경우 부분적인 wetting만이 이루어져 소결초기에서는, 팽창현상이 소결촉진효과 보다 크게 작용하여 치밀화에 역행하지만, 이 단계가 지나면 점차 치밀화가 이루어짐을 알 수 있었다. 그리고 소결압분체들의 산화는 기공을 중심으로 하여 진행됨을 볼 수 있었다. 한편 산화온도 1,000℃이하에서는 이들 소결체의 표면산화물이 보호피막으로 작용됨을 알았고, 1,100℃ 이상에서는 보호피막으로서 유효하지 못함을 알았다. This study is a powder metallurgy of Fe-Al alloy which is used for oxidation resistance at high temperature. The material used in this investigation was Fe-(6~l2wt.%) Al powder compacts, and sintering and oxidation behavior of the material at high temperature have been examined. The compacts were comported of pure iron powder and Fe-50% Al alloy powder so as to the final Al contents of the compact to be 6, 8, 10 and 12wt.%. The compacts were premised under 7ton/cm2 and sintered at various conditions (sintering temperature; 1,100℃, 1,200℃ and 1,300℃: sintering time; 10 to 300min.). The analysis of sintering behavior has been undertaken by the methods of porosity measurements, metallographic examinations and the calculated densification parameters. Also the sintered compacts were oxidized in air at various temperatures(900℃, 1,000℃, and 1,100℃) from 1min. to 15hr., and the oxidation behavior was analyzed by the weight gain measurements and metallographic examinations. The results obtained in these experiments can be summarized as fellows: 1) It was found that the solid state sintering at 1,100℃ did not bring about the densification, because of the continuous expansion by alloying at contact point. 2) The liquid phase sintering at 1300℃ resulted in only partial wetting, and the densification was diminished by the predominant expansion rather than the shrinkage at the initial stage. Thereafter the densification began due to the formation of homogeneous phase when the liquid phase was disappeared. 3) The oxidation of the sintered compacts was found to be proceeded at pore sites and was affected by sintering conditions as well as Al contents. 4) The oxidation below 1,000℃ is obeyed the parabolic rate law. Meanwhi1e the oxidation at 1,100℃ is facilitated linearly and the oxide layer is not likely to be effective as a protective layer above this temperature.

고흥반도 연안에 분포하는 浮遊性 卵·仔稚魚 종조성의 계절변동

한경호,신영호,황동식 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.1

전라남도 고흥군 녹동연안에서 2000년에 계절별로 총 4회에 걸쳐 만조시 채집된 浮遊性 卵·仔種魚의 종조성 및 양적변동을 조사하였다. 조사기간 동안 채집된 浮遊性 卵은 총 8개 분류군이 출현하여 7개 분류군은 種 수준까지, 1개 분류군은 기타난으로 동정되었다. 그 중에선 멸치(Engraulis japonica)가 전체 출현량의 52.1%를 차지하여 가장 우점하였으며, 다음으로 기타난이 13.2%, 주둥치(Leiognat husnuchalis)가 12.3% 보리멸(Sillago sihama)이 8.6%, 앨퉁이(Maurolicus muelleri) 4.0%, 전어 (Konosirus punctatus) 1.0%, 참서대(Cynoglossus joyneri) 0.2%을 차지하였다. 仔種魚는 총 8目 26科 33屬 37개 분류군의 仔種魚가 출현하여,33개 분류군은 種 수준, 2개 분류군은 屬수준, 2개 분류군은 科 수준까지 동정되었다. 조사기간 동안 출현한 仔種魚 중에서 멸치가 31.7%를 차지하여 최우점하였으며, 망둑어科(Gobiidae)에 속하는 어류는 46.2%, 베도라치(Pholis nebulosa)와 실고기(Syngnathus schlegeli)는 각각 5.4%와 2.3%를 차지하였다. 조사기간 동안 출현한 仔種魚 중에서 이들 4개 분류군이 차지한 비율은 85.6%를 차지하였다. The ichthyoplankton were sampled during four different months (2000) to study their seasonal variations and species composition off Kohung Peninsula. During the study, the fish eggs were identified to eight taxa, seven of which were identified to species level, and one identified to order. The most dominant species. Engraulis japonicus, accounted for 52.1% of the total fish eggs, followed by Leiognathus nuchalis(4.0%), Sillago sihama(8.6%), Maurolicus muelleri(8.6%), Sardinops melanostictus(4.0%), Konosirus punctatus(1.0%), Cynoglossus joyneri(0.2%). The collected larvae and juveniles were identified to 37 taxa (33 genera, 26 families, 8 orders). Of these, 33 were identified to species level, and two were identified to genus and family level, respectively. The dominant species, Engraulis japonicus, accounted for 31.7% of the total larvae and juveniles; it was followed by Gobiidae(46.2%), Pholis nebulosa(5.4%) and Syngnathus schlegeli(2.3%). These four taxa constituted 85.6% of the total collected larvae and juveniles.

다발성으로 피부절을 수반한 대상포진 뇌수막염 1예

한동철,박종호,신원철,이영배,박현미,신동진 대한노인병학회 2004 대한노인병학회지 Vol.8 No.1

저질에 따른 꼬막(Tegillarca granosa)의 염분내성

한경호,진동수,추은경,이우범,이원교 國立 麗水大學校 環境問題硏究所 2000 環境硏究論文集 Vol.3 No.-

The present study was conducted to know the salinity tolerance of the bloody clam, Tegillarca granosa (Linnaeus) caught in Gang-jin Bay from May to June in 2000. In the experimental groups of sandy-mud, the survival and infiltration rate were not bad at 20~40ppt, but all the bloody clam, Tegillarca granosa died at more than 50ppt and less than 10ppt before the experiment finish. In the experimental groups of muddy, the survival and infiltration rate wrer not bad at 20~35ppt and then 40ppt, 45ppt. All the bloody clam, Tegillarca granosa died at more than 50ppt and less than 10ppt. The infiltration rate was high at 25~35ppt in the both of two experimental groups. In the sandy-mud, the bloody clam, Tegillarca granosa infiltrated less than half of their shells at 25ppt, 30ppt but in the muddy, they infiltrated more than half of their shells at 25ppt, 30ppt, 35ppt.