HMG-CoA 환원효소 억제제에 의한 ICAM-1 유전자의 발현조절

김현진,정효균,홍우정,김군순,조영석,김도희,채수흥,구본정,송민호,노흥규,김영건 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : ICAM-1 act as one of major adhesion molecules in the atherosclerotic lesion. ICAM-1 expression is mainly regulated at the level of transcription and depend on IFN-γ signal transduction pathway in which the STAT1 transcrption factor is a critical intermediate. IFN-γreceptor not only initiates tyrosine 701 phosphorylation of STAT1 by Jak1 and Jak2, but also phosphorylates serine 727 through the activation of Raf-1/MAP kinases. HMG-CoA reductase inhibitors have anti-atherosclertic effects, beyond normalization of hypercholesterolemia, by directly acting on endothelial cells, macrophages and vascular smooth muscle cells. HMG-CoA reductase inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibition posttranslational farnesylation and geranyl-geranylation processing of small GTP-binding preoteins and inhibition of normal signaling activities. Method : We made several 5'-deletion constructs of rat ICAM-1 promoter and analyzed the promoter activities by measuring the luciferase activity after transfection into ECV304 cells and smooth muscle cells. We checked the level of total and phosphorylated STAT1 protein by immunoblot analysis using specific antibodies. Results : Lovastatin inhibits IFN-γ-induced ICAM-1 gene expression in the ECV304cell. The cells pretreated with PD98059, MEKK inhibitor showed significantly low ICAM-1 RNA induction with IFN-γ stimulatio. IFN-γ induced phosphorylation of tyrosine 701 was not significantly changed by the pretreatment of lovastatin. But lovastatin suppresses IFN-γ-induced phosphorylation of ERK1/ERK2 which are responsible for the seine 727 phosphorylation in STAT1. Conclusion : We showed that HMG-CoA reductase inhibitors, lovastatin, suppresses IFN-γ mediated ICAM-1 gene expression through the inhibition of transcription. HMG-CoA reductase inhibitor suppresses IFN-γ-induced phosphorylation of serine 727 in STAT1 through the modulation of MAP kinases.

개에서 발생한 폐수종의 치료 증례

김유수,심재현,김건우,김지용,이병희,송근호,이영원,김덕환 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 9 months intact male American bull dog with chief complaints of dry cough, dyspnea and fever was referred to the Veterinary Teaching Hospital, Chungnam National University. Based on the findings of physical examination, radiological examination, and blood and blood chemical examinations, the present patient was diagnosed into pulmonary edema secondary to primary bacterial and aspiration pneumonia. The patient was hospitalized and treated with buscopan, cephazolin and furosemide for 1 week. His activity and appetite was returned to normal and thoracic radiographs revealed that Jung was recovered to the normal condition after treatment for 7 days. The present patient was a case with pulmonary edema showed favorable therapeutic responses to proper medication.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

갑상선 세포에서 전사보조활성인자인 CBP와 CIITA의 인터페론-감마 활성 부위에 대한 조절 : CBP and CIITA on GAS(Interferon- γ Activated Site)-Mediated Transcription in Thyroid Cells

채수흥,홍우정,김군순,조영석,김도희,김현진,구본정,송민호,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : In the previous stuides, we identified that the interferon-r activated sequence (GAS) in the 5-flanking region of rat ICAM-1 gene is major element for interferon-r-inducible expression of the gene in rat thyroid cells, FRTL-5. We here, investigated the role of transcriptional coactivators, CBP(CREB binding protein) and CⅡTA (class Ⅱ transactivator) in the modulation of the activity of GAS which could interacts with signal transducers and activators of transcription-1 and 3 (STAT1 and STAT3). Methods : The expression of CBP RNA and protein were quantitated in FRTL-5 after stimulation with interferon-r (IFN-r), thyroid stimulating hormone (TSH), forskolin and methimazole. Direct association of CBP with STAT were analyzed by immunoprecipitation. The transcriptional roles of CBP and CⅡTA in the regulation of GAS were assessed by the cotransfection with their expression vectors with reporters; 5-deletion constructs of rat ICAM-1 promoter or 8xGAS-luc constructs, into FRTL-5 thyroid cells. Results : The level of CBP RNA and protein were not changed by the treatment with TSH, IFN-r, forskolin and methimazole in FRTL-5, FRT and BRL liver cells. The CBP could be directly associated with STAT1. Furthermore, the overexpression of CBP significantly increases the both promoter activities; rat ICAM-1 gene promoter which has GAS element and 8xGAS-luc cassette constructs. However, the cotransfection of CⅡTA decreased the constitutive and CBP-mediated transactivation of rat ICAM-1 promoter and 8xGAS-luc cassette constructs. Conclusion : We identified that the two tanscriptional coactivators; CBP and CⅡTA has differential roles in the regulation of transcriptional activity of GAS drived promoter. CBP increases the GAS activity through the direct binding with STAT1, but CⅡTA inhibited the CBP-mediated transactivation of GAS activity.

면역억제제를 사용치 않은 동소성 간이식 실험견의 조직병리소견

조원현,최순옥,김인호,임태진,김성수,박준희,김우석,김현철,최규택,김진모,권건영 大韓移植學會 1994 Korean Journal of Transplantation Vol.8 No.1

채널할당을 위한 효울적인 유전자 알고리즘

김성수,김근배 江原大學校 産業技術硏究所 2002 産業技術硏究 Vol.22 No.B

The objective of this paper is to develop an efficient genetic algorithm (GA) to find a channel assignment method for minimum interference among the channels within reasonable time. The series of specific channel number is used as a representation of chromosome. We use minimum-channel-distance encoding scheme within the same cell to consider cosite channel interference (CSI) when chromosomes are generated. The cell base crossover is also used. This proposed method improves solution quality within limited time.

인체 대장암에서 72kDa 제 4형콜라겐 분해효소의 발현

김정은,손건영,전수한 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1

수술 절제한 대장암 45예에서 Northern blot analysis와 gelatin zymography를 시행하여 72kDa 제 4 형콜라겐 분해효소의 mRNA치와 활성도를 조사하였다. 대장암에서 주위 정상조직에 비해 이 효소의 mRNA치가 증가하였으며 단백질 합성단계에서 효소의 활성도와도 연관되었다. 특히 활성형인 62kDa의 활성도가 크게 증가하였다. 암진행에 따른 이 효소의 활성도 변화는 T2와 T3에서 암조직과 정상조직사이에서 mRNA치와 효소활성도에 차이가 있었으나 전반적으로 암의 침윤도, 림프절 전이, 듀크스 병기에 따른 유의한 차이는 볼 수 없었다. 암침윤과 전이에는 제 4 형콜라겐 분해효소 외에 딴 metalloproteinase들, plasminogen activator등 여러 가지 다른 단백질 분해효소도 관여하며, 암종류에 따라 이들 효소의 작용정도가 차이가 날 수 있다. 또 조직 억제제인 tissue inhiitors of metalloproteinase(TIMPs)와의 균형관계도 고려해야 한다. 그러므로 대장암에서 72kDa 제 4 형콜라겐 분해효소의 증가된 활성양상을 이들 다른 인자들과 함께 관찰하면 암의 진행정도를 나타낼 수 있는 보다 뚜렷한 지표를 만들 수 있을 것으로 생각된다. Type Ⅳ collkagenases are thought to play a role in the proteolysis of the basement membrance and the extracellular matrix by the malignant cells that occur early in the development of invasive tumors. In several human tumors, including breast, colonic, ovarian, and gepatocellular carcinomas, Type Ⅳ collagenase expression has been positively correlated with tumor invasiveness. In this study, we examined the expression and the activation of 72kDa Type Ⅳ collagenase in cancerous and normal tissues of the colon and, we attempted to clarify its clinical significance in 45 cases of human colorectal cancer by using northern blot analysis and gelatin zymography. This study showed that the level of 72kDa Type Ⅳ collagenase mRNA was increased in colorectal cancer and that this increase was associated with increased enzymatic activity. While there was a difference in the expression and the activation of this enzyme between T2 and T3, this enzyme activity did not show a definite correlation with cancer progress as measured by the depth of tumor invasion and lymph node metastasis.

병원표준화심사의 질 향상 관련 항목에 대한 평가와 개산방안

김창엽,이상일,이건세,신영수 한국의료QA학회 1995 한국의료질향상학회지 Vol.2 No.1

Background: Korean Hospital Standardization Program(KHSP), as a hospital accreditation program initiated and promoted by Korean Hospital Association since 1981, has played a key role in the hospital quality improvement in structural aspect particularly. Recently, however, KHSP has been critisized by hospitals and health personnels in that it is unpractical to be utilized as an initiative to improve hospital quality. In particular, the section of quality assurance in KHSP, strongly influenced by old version of Hospital accreditation of Joint Commission in U.S.A., has been required to be fundamentally sevised. For design of new criteria in quality assurance section, a survey for evaluation of existing program and collection of opinions for ideal one was conducted. Methods: For a month in 1994, structured questionnaires were administered by mailing to 470 hospital staffs from 200 hospitals, participated in the survey for the 1994 KHSP in each hospital. The total number of respondents was 116. Results: Less than half of the respondents(34.5%) value positively on the impact of current KHSP in general on the quality improvement of their hospitals. Moreover, most responses indicated that KHSP should be reorganized towards more practical and applicable one. Current KHSP criteria for quality activities in hospitals were regarded as a unpractical one which should be basically renewed. For new criteria and standards, most respondents emphasized the importance of applicability of those in real siuations. Conclusion: For the KHSP to be effective, new evaluation criteria for quality activities should be more practical and fully accommodated to hospital situations in reality.

Treadmill 운동부하시 비만중년 여성의 유산소능력, 심근산소소비 및 심전도 ST의 변화

김종덕,최건식,황수관 대한스포츠의학회 1994 대한스포츠의학회지 Vol.12 No.2

The purpose of this study was to examine changes in aerobic capacity, MV´O_2, and ST segment at electrocardiography during treadmill exercise. Forty-six obese women, ranged in age from 40s-50s yr, were divided into four groups; 1) Contorl group 1(N=10, body fat < 35%), 2) Cotrol group 2(N=12, 25 < body fat <30%), 3) obese group 1(N=11, 30% < body fat <35%), and 4) Obese group 2(N=13, 35% < body fat). The findings of this study were as follows. 1. Subjects with higher percent of body fat showed lower LBM and percent of body fluid. 2. Every group showed a significant increase in HR after one minute of exercise, and gradual increase in HR during the first 2 of 3minutes of exercise. Increase in heart rate of obese group 2 was higher that that of other groups during exercise. 3. The blood pressure of obese groups both at rest and during exercise was higher than that of control group. 4. There was no big difference in V´O_2 between four groups at rest and before 5 minutes of exercise. After 5 minutes of exercise, V´O_2 of obese group 2 increased significantly. Subjects who had higher percent of body fat showed lower V´O_2max. 5. There was no big difference in energy expenditure between four groups at rest of during 5 minutes exercise. After 5 minutes of exercise, energy expenditure was significantly increased in obese group 2. 6. The MV´O_2 of each groups after one minute of exercise increased twice or more than that of the same group at rest. The MV´O_2 of obese groups was larger than that of control groups. 7. The Regression equations between HR per minute and SBP, V´O_2 and MV´O_2 were obtained from the study. The Regression equations of HR per minute was significantly correlated to SBP, V´O_2, and MV´O_2. 8. The ST level of each group. during exercise, decreased approximately 50% of the same group at rest, while the ST slope increased approximately twice of three times of the same group at rest. The study found two interesting facts: firstly, LBM and body fluid were inversely proportional to percent of body fat. Furthermore, there was a small difference between control groups and obese group 1, while big difference existed between control groups and obese group 2. It is noted that BP, V´O_2, and MV´O_2. could be measured only from HR per minute using regression equations. Moreover, ST level decreases while ST slope increases.

건축공간에서 창에 의한 관념적 공간인지에 관한 연구

김정환,오건수 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.2

Concept of space is one of endless discussion topics and research's targets until now a days from ancient time. Concept of space in architecture was well-known in late 19th century, and was used as a important principle. Many architects made architectural space from the basis of these concept of space, and made ideation space through window. This study is to interpret existence and possibility of such space called ideological cognitive space which is shown through window.