다회혈장성분헌혈이 헌혈자에 미치는 영향

이미경,황유성,이규범,한희숙,김재호,민원기 大韓輸血學會 1998 大韓輸血學會誌 Vol.9 No.1

국내에서 발생하고 있는 사람 브루셀라증의 임상적 특성

김연숙,최영실,오원섭,권기태,이혁,이선희,손준성,김신우,장현하,정숙인,고관수,박미연,백경란,송재훈 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

배경 : 2002년 내국인에서의 첫 사람 브루셀라증의 증례 보고 이후 국내에서 사람 브루셀라증이 빠른 증가를 보이고 있다. 이에 연구자들은 국내에서 발생하는 브루셀라증의 임상적 특성을 연구하고자 하였다. 방법 : 2003년부터 2006년 7월까지 7개 대학병원에 내원한 환자들을 대상으로 WHO의 진단기준에 의하여 확진된 브루셀라증 환자들의 의무기록을 후향적으로 조사분석 하였다. 배양된 균은 삼성서울병원에 의뢰하여 16S rRNA 유전자 분석방법을 이용하여 종을 확인하였고, 표준시험관응집검사는 국립보건원에 의뢰하여 시행하였다. 결과 : 34예의 환자들이 브루셀라증으로 확진되었고, 31명이 남자였다. 혈액에서 B. abortus가 증명된 경우가 8예, 표준시험관 응집검사상 의미 있는 항체가 상승을 보인경우가 26예이었다. 환자들은 대부분 축산업관련 종사자였고, 추후 브루셀라증으로 확진된 소와의 직접 접촉을 통해 감염된 것으로 여겨진다. 국내 사람 브루셀라증의 가장 흔한 증상은 발열이었고, 위장관계 합병증이 가장 흔히 동반되었다. 치료로는 doxycycline/rifampicin이 24예의 환자에서 doxycycline/aminoglycoside (streptomycin 혹은 gentamicin)이 10예의 환자에서 6주 이상의 기간 동안 투여되었다. 치료에 반응을 보이지 않는 환자는 없었고, 재발이 1예 있었다. 결론 : 국내에서 발생하는 사람 브루셀라증의 원인균으로 현재까지 분리 동정된 것은 B. abortus이며, 소 브루셀라증의 증가로 인해 인체감염사례가 함께 증가하는 것으로 사료된다. 위험 직업군 종사자들에서 감염된 소와의 직접 접촉을 통해 주로 발생하고 있는 브루셀라증의 임상종상은 국외에서 발생하는 경우와 유사한 양상을 보이고 있으며 치료에 대한 반응은 비교적 좋은 편이긴 하나, 추후 보다 많은 환자들을 대상으로 한 연구가 필요하리라 사료된다. 사람 브루셀라증의 조절을 위해서는 소 브루셀라증의 조절이 필수적이며, 브루셀라증의 조절에 성공한 선진국의 사례를 적극적으로 도입해야 할 것으로 생각된다. Background : Since the first Korean case of human brucellosis was reported in 2002, the incidence of human brucellosis has been rapidly increasing. We performed this study to evaluate the clinical characteristics of human brucellosis occurring in South Korea. Materials and Methods : Demographic features, clinical manifestations, and therapeutic outcomes of cases confirmed as brucellosis according to WHO diagnostic criteria were evaluated. Species of Brucella isolates were identified with 16S rRNA gene sequencing method. Serologic diagnosis was performed with standard tube agglutination test (STA). Results : A total of 34 cases were enrolled from 2003 to 2006 in six hospitals. Eight cases were confirmed with the isolation of Brucella from blood and 26 cases were confirmed with titer of antibodies ≥1:160 by STA. Male (31) was predominant and most of the patients were cattle farmers. The most common transmission route was direct contact with infected or their cattle byproducts. Fever was the most prominent symptom and fatigue followed. Gastrointestinal and musculoskeletal systems were commonly complicated. Patients were treated with doxycycline and rifampin or aminoglycosides for more than 6 weeks. All patients responded well to the therapeutic regimens, but one patient relapsed 10 months after treatment. Conclusion : Brucellosis occurring in Korea has been caused by B. abortus, whose preferred natural host animals are cattle. Korean patients were infected through direct contact with bovine brucellosis. Clinical manifestations of brucellosis were similar to those occurring worldwide. Therapeutic outcomes were good among patients with brucellosis in Korea. In order to prevent human brucellosis, measures to eradicate bovine brucellosis should precede.

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

중학교 과학교육과정에서 수행평가의 일반적 사항과 적절한 수행평가 방법에 관한 과학 교사들의 인식 조사

김성원,현미숙 이화여자대학교 사범대학 교과교육연구소 2005 교과교육학연구 Vol.9 No.2

중학교에서 수행평가가 공식적으로 도입된 이래, 아직 수행평가가 정착되지 않아서 어려움과 문제점들이 많이 지적되고 있다. 수행평가는 많은 교육적 효과를 가지고 있지만 교사가 시행하는 방법에 따라서 효과가 달라질 수 있다. 그러므로 교사가 수행평가의 주제와 적절한 방법을 선정하는 것은 수행평가의 성패를 좌우하는 중요한 일이라 할 수 있다. 본 연구에서는 서울·경기 지역의 중학교 20개교에 재직 중인 과학교사 80명을 대상으로 수행평가의 일반적 사항과 중학교 과학에서 각 단원별로 적절한 수행평가의 방법에 대한 교사들의 인식을 조사하였다. 연구 결과, 교사들은 수행평가의 과제 내용으로 내용과 과정을 통합하는 것과 과학적 원리와 개념을 통합하는 것이 중요하며, 과제 선정시에는 학생들이 배워야 할 지식과 기능을 고려해야 한다고 인식하고 있었다. 수행평가의 과제 수행을 통해 학생들이 얻을 수 있는 효과로 정보를 얻기 위해 다양한 기능을 사용할 수 있게 된다는 점을 들었다. 수행평가를 포함한 학습평가에서 교사들은 타당도와 신뢰도를 매우 중요시 여겼다. 과학교육과정 중 물리 단원에서는 서술형 검사법과 실험·실습법이 적절하고, 화학 단원에서는 실험·실습법과 서술형 검사법을, 생물 단원에서는 서술형 검사법이 적절하며, 지구과학 및 환경 단원에서는 서술형 검사법, 연구 보고서법, 포트폴리오법이 적절한 것으로 교사들은 인식하고 있었다. Since performance assessment is introduced officially, many difficulties and problems are pointed out, because it is not settled down yet. It has many educational effects. But effects can be changed by methods of teacher's operation. So, it is important things that teachers select theme and appropiate methods of performance assessment. This study researched the recognition of science teachers about the general matters of performance assessment and the appropiate performance assessment methods. The results of this study is as follows. First, teachers replied that the work contents must be combined with the contents and processes, scientific principles and concepts. Teachers replied that work contents of performance assessment must be selected considering of knowledge and ability which the students learned. Second, teachers replid that learning evaluation including of performance assessment must be avalible and reliable. Third, essay test and experimental test were most proper in unit of physics of middle school science. Experimental test was proper in unit of chemistry. Essay test was proper in unit of biology. Essay test, research paper test and portfolio evaluation were proper in unit of earth science and environment.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

소화기암환자의 항암요법 시간 경과에 따른 피로도 측정 연구

박지원,김용순,서미숙 성인간호학회 2001 성인간호학회지 Vol.13 No.4

Fatigue is one of the most common complaints of cancer patients. In this study, we analyzed the change of fatigue level and general symptoms as time go by, so that, we could explain more on the mechanism and chance of fatigue in relation with treatment, and explore the influencing factors. Method: The subjects of this study were 50 GI cancer patients who have visited the cancer center of A hospital in Suwon. We measured fatigue by using the Revised Piper Fatigue Scale(RPFS) at the time of starting and finishing induction chemotherapy, and starting the 2nd cycle of chemotherapy. Results: 1) The fatigue score was 2.81, 3.73, and 3.82 in a 10 point scale at the time of starting and finishing induction chemotherapy, and starting the 2nd chemotherapy, respectively. This means fatigue persisted until after the treatment. 2) Fifty two percent of participants complained of some kinds of symptoms when starting the treatment, and the proportion increased up to 92% when finishing the treatment. 3) Fatigue scores were significantly high in patients with fatigue-related symptoms than for patients without those symptoms. 4) Fatigue scores showed significant differences according to patients' general characteristics such as age, educational level, economic status, occupation, diagnosis, hematocrit, weight, and amount of sleepy. Conclusion: We have to develop intervention strategies to reduce fatigue in cancer patients in the consideration of influencing factors.

6-아닐린피리다진 유도체 제조의 최적반응

오미정,원용훈,박명숙 덕성여자대학교 약학연구소 2006 藥學論文誌 Vol.17 No.-

The 3-allylthio-6-anilinopyridazine(3) was synthesized by allylthiolation and amination in order to discover antitumor candidates. We considered a reaction condition like solvent, catalyst and reaction time in order to find optimal reaction for the preparation of 3-allylthio-6-anilinopyridazine(3). 3-Allylthio-6-chloropyridazine(2) was prepared from the reaction of 3,6-dichloropyridazine(1) with allylmercaptan and sodium hydroxide. The aniline moiety was introduced into 6-position of pyridazine ring of compound 2. The aminations for 3-allylthio-6-anilinopyridazine(3) were performed by refluxing for 3h in n-butanol with NH_(4)Cl(method A), 5h in ethanol with NH_(4)Cl(method B) and 6h in n-butanol with Cu powder(method C). The combination of NH_(4)Cl/n-butanol/3h was found to provide a convenient method for giving 3-allylthio-6-anilinopyridazine in satisfactory yields.

Emulsion형 化粧品의 Escherichia coli 汚染防止에 대한 防腐制效果

柳美淑,金樟圭,元省皓,金男紀 成均館大學校 科學技術硏究所 1992 論文集 Vol.42 No.2

Most of cosmetics are emulsion products that contain the source of nutrition-vegetable oil, mineral oil and carbohydrate etc. -so there is much possibility of contamination by microbials. The purpose of this study is to get the data necessary not only to prevent dermatopathia occurred by Escherichia coli but to sustain the quality and the wear of cosmetics as the result of finding optimum growth condition and the growth condition of Escherichia coli for enviro-factor, utilizing antiseptic so as to prevent contamination by observing it in emulsion type cosmetic. The results are as follows : 1. The optimum growth condition of Escherichia coli : pH7.0 and 37.5℃ The mixing volume ratio of water / oil was 70/30 in this experiment. 2. Adding antiseptics into emulsion type cosmetic, the number of Escherichia coli was decreased from 10^8(cell/ml) to 8 x 10^3(cell/ml) 3. Antibacterial effect against Escherichia coli was better as follows, p-hydroxy benzoic acid propyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid buthyl ester + acetic acid buffer solution > p-hydroxy benzoic methyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid methyl ester + p-hydroxy benzoic acid buthyl ester >p-hydroxy

교정환자의 브라켓과 치아 경계부에 존재하는 치면세균막내 mutans streptococci 종 및 생물형의 식별

김미애,유소영,김화숙,국중기,임성훈,윤영주,김광원 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.1

본 연구는 교정환자의 브라켓과 치아 경계부 및 브라켓으로부터 2 mm 이상 떨어진 치아 평활면의 치면세근막에 존재하는 mutaus streptococci의 종 및 생물형에 차이가 있는지를 알아보고자 시행되었다. 조선대학교 치과병원에 내원한 13세 이상 35세 미만의 환자 28명으로부터 브라켓을 장착하고 있는 61개 치아에서 치균세균막을 채취하여 mutans streptococci를 MSB 배지에서 선택적으로 분리한 다음, 이들의 지놈 DNA를 추출하여 dextranase 유전자를 표적으로 하는 중합효소연쇄반응법을 시행하고, 그 증폭물을 Hae Ⅲ로 소화하고, 이를 전기영동하여 제한효소절편길이에 따라 그 종을 식별하였다. 또한 생물형을 조사하기 위하여 생화학적 검사를 실시하였다. 그 결과 브라켓과 치아 경계부 및 브라켓으로부터 2 mm 이상 떨어진 평활면의 치면세균막에 존재하는 mutans streptococci 종은 서로 비슷한 검출 빈도를 보이나 두 곳에 존재하는 mutans streptococci 생물형은 서로 차이가 있는 것으로 나타났다. 향후 브라켓과 치아 경계부 및 치아 평활면의 치면세균막의 mutans streptococci 생물형의 차이와 브라켓 주위의 법랑질 탈회 및 치아우식증 발병과의 상관관계에 대한 연구가 필요하다. The aim of this study was to compare the species and biotypes of mutans streptococci isolated from dental plaques sampled from the interfaces between the bracket and tooth surface and smooth tooth surfaces in orthodontic patients. Dental plaque was collected from the interfaces between brackets and teeth (test group), and from smooth tooth surfaces distant from brackets by more than 2 mm (control group). The dental plaque collected by a sterilized curette was transferred into a vial of 1 X PBS. The sample in the vial was vigorously vortexed for1 min and plated on mitissalivarius bacitracin (MSB) agar plate using cotton tips. The agar plates were incubated at 37℃ in a candle jar for 2 days, and again incubated for 1 more day at anambient temperature. Individual colonies were cultured in TH broth at 37℃ CO₂ incubator. The PCR-RFLP based on dextranase gene was performed for the identification of mutans streptococci at the species-level. For biotyping of mutans streptococci, biochemical tests were performed. There was no significant difference of the species of mutans streptococci isolated from both test and control groups. However, the biotypes of the mutans streptococci isolated from test and control groups were different. These results may offer the basic data to verify the relationship between the mutans streptococci biotype and enamel decalcification or dental caries in orthodontic patients with fixed appliances.

全羅南道 保健診療員의 1次保建醫療事業에 關한 硏究

李榮淑,朴梧張,金貞仁,姜惠英,金美園,崔順姬,金修辰 全南大醫大 1982 전남의대학술지 Vol.19 No.4