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북동태평양 Clarion-Clipperton 균열대의 망간단괴 채광을 위한 환경충격시험 예정 지역 심해 해저면에 서식하는 중형저서생물 현존량 및 공간 분포 특성
민원기,노현수,김동성 한국환경과학회 2020 한국환경과학회지 Vol.29 No.11
This study investigated the distributional pattern of meiobenthos associated with future deep-sea mining in the Korea Deep Ocean Study area present in the Clarion-Clipperton Fracture Zone (CCFZ) located in the southeastern part of the North Pacific Ocean. Standing stocks of meiobenthos were investigated in benthic impact experiment sites (BIS) and Korea Institute of Ocean Science & Technology long-term monitoring (KOMO) sites during the 2008-2014 annual field survey. A total of 14 taxa of meiobenthos were identified. Nematodes were the most abundant taxon (60-86%). Harpacticoid copepods (5-26%) and benthic foraminifera (1-12%) were also dominant at all sites. The total meiobenthic densities varied from 4 to 150 ind./10 cm2. The mean value of total meiobenthic abundance was higher at BIS than at KOMO sites, but there was no significant difference between the two sites. The mean values of the number of taxa and biomass at BIS and KOMO sites were similar. The mean abundance of nematodes that were the most dominant taxa was also higher at BIS than at KOMO sites. The standing stocks in our study sites were relatively lower than those previously reported at other CCFZ sites. These results seem to reflect a low organic concentration in the study area.
Production of Single Chain Antibody against Mycotoxin Aflatoxin B1 in Recombinant Escherichia coli
민원기,조영진,최규호,권대혁,손동화,박경문,서진호 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.1
Aflatoxin B1 (AFB1) is a secondary metabolite produced mostly by Aspergillus flavus strain and toxic to human and several animal species as food contaminant. The single chain variable fragment (scFv) genes of a monoclonal antibody (mAb) against AFB1) were cloned and expressed in recombinant Escherichia coli. A fusion of myeloma cells and spleen cells from the immunized mice, hybridomacells were cultured to obtain anti-AFB 1 mAb of 1.3 ng/ml IC50and 0.1 ng/ml detection limit. Complementary DNA was constructed by the reverse transcription-polymerase chain reaction and DNA sequence analysis identified that each variable region was composed of the heavy chain variable region (VH) as a type of IgG1 and the light chain variable region (VL) as a type of λ. OverlapextensionPCR using linker encoding polypeptide (Gly4Ser)3 led to combination of VH and VL genes and expression in recombinant E. coli. Co-expression of molecular chaperones enhanced soluble expression of anti-AFB1 scFv and the ubiquitin fusion system increased expression levels. In vitro refolding was performed and competitive direct enzyme linked immunosorbent assay determined the optimized condition.