서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

유리 성형용 금형의 균열 발생 기구

황규홍,송동환,양권승,이종국 조선대학교 생산기술연구소 2005 生産技術硏究 Vol.27 No.2

Microstructure of glass-forming mold have influence on the mechanical properties such as hardness, and life span of the glass-forming mold is strongly dependent on its microstructure. The glass-forming mold with guaranteed tool life requires fine particle size and homogeneous distribution of Cr precipitates in the matrix. In this study, mechanism on the crack creation of the glass-forming mold made in Korea (K-mold) was compared to the one glass-forming mold made in Japan (J-mold). SEM observation revealed that the particle size of the Cr precipitates in J-mold was smaller than that in K-mold, in addition the size distribution of J-mold was narrower than that of K-mold, resulting in long life of J-mold. Therefore, it was confirmed that the microstructure of the glass-forming mold related to Cr precipitates was one of the important parameters to improve the quality of products.

필기문서의 온라인과 오프라인의 인식에 관한 연구

黃仁鎭,鄭成鐘 全北大學校 1992 論文集 Vol.34 No.-

A method to recognize mixed documents of Hangul, Alphabet, and digits is proposed in this thesis. The recognition system included on-line and off-line and off-line process for automatic input system. On-line recognition system is implemented by finite automaton and would be possible to process in real-time. For off-line recognition of Hangul, Alphabet, and digit, graph patterns of characters are generated and transformed into tree patterns. these tree patterns are searched by preorder-traversal to generate recognition key. Recognition of mixed document is completed by searching recognition rule table by these keys.

생후 등줄쥐의 대뇌겉질 및 줄무늬체에서 NADPH-diaphorase 양성신경세포에 관한 연구

황승준,이종환,홍혜남,이흥식,허영범,조정식 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

두가지 혈류 유발방법에 의한 새로운 심폐소생술(이중 혈류 유발 심폐소생술)장치의 개발

황성오,김현,조준휘,오범진,임종천,최경훈,윤정한,이승환,김영식,이강현,이윤선 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: There have been many efforts to augment blood flow during cardiopulmonary resuscitation. These efforts have focused on maximizing the effect of cardiac pump or thoracic pump alone. However, considering that the heart is the biggest blood reservoir and increase of intrathoracic pressure can generate blood flow, simultaneous exploitation of both mechanisms may have synergistic effect. We hypothesized that simultaneous chest constriction in addition to sternal compression by standard CPR may have additive hemodynamic effects by preventing deformation of the chest and increase of intrathoracic pressure. Methods and results: we built a new mechanical device to perform compression and thoracic constriction simultaneously. The device consists of two main elements. Piston in the center is to depress the sternum. Strap is to constrict the thorax circumferentially. Strap is attached to both sides of the piston. When the piston is pushed down, it depresses the sternum and pulls on the thoracic strap. To determine strap width to produce optimal hemodynamic effect, we measured hemodynamic parameters with variable widths of strap in two dogs after induction of ventricular fibrillation. Result of the experiment showed that 10cm wide strap was determined to be most effective. We also determined optimal depth of compression to produce maximal hemodynamic effect with animal experiments using two dogs. Animal experiments showed that the highest aortic pressure could be generated when the stemum was depressed to 5 cm. Cardiopulmonary resusciation using a new device could generate higher systolic aortic pressure, coronary perfusion pressure and end-tidal carbon dioxide tension in comparison with standard cardiopulmonary resuscitation in a pilot animal study using two dogs. Conclusion: New cardiopulmonary resuscitation method using a mechanical device designed by us could perform sternal compression and simultaneous thoracic constriction, and generate better hemodynamic effects than standard cardiopulmonary resuscitation in pilot animal experiments.

T1 강조 영상에서 선조체 고신호강도를 보이는 당뇨 환자에서의 편측무도증

정승철,이병철,김상윤,배재천,황성희,도화범,박종호 대한신경과학회 1997 대한신경과학회지 Vol.15 No.1

2종 전자근관장측정기의 일관성에 관한 in vitro 연구

황규용,노병덕,김의성,이승종 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.1

정확한 근관장 측정은 성공적인 근관치료를 위해 필수적이다. Kuttler는 근관협착부는 주근단공에서 0.5 mm 상방에 위치한다고 하였고 Lee는 상아백악질경계점부위보다 주근단공이 재현성이 높고 임상적으로 관찰하기 용이하다고 보고하였다. 본 연구는 alginate model상에서 2개의 전자근관장측정기를 사용하여 얻은 측정치의 정확성(accuracy)을 평가하고 각각 0.5 mark와 Apex mark중에서 어느 지점에서 더 일관성(consistency)을 보이는지를 비교하고 또한 근관장을 측정할 때 파일을 전진시키면서 Apex mark를 측정한 값과 치근단부위를 지나친 후 다시 후퇴하면서 측정한 값의 차이를 비교하고자 하였다. 52개의 발거된 건전한 제 1, 2소구치를 대상으로 하였으며 Root ZX와 E-Magic Finder Deluxe를 이용하여 각각 26개중 13개는 파일을 전진하면서 0.5 mark에서 근관장을 측정하고 Apex mark에서 근관장을 측정한 후 0.5 mark에서 고정하였다. 나머지 13개는 0.5 mark에서 근관장을 측정한 후 Apex mark에서 근관장을 측정하고 다시 치근단 부위를 넘어선 후 후퇴하면서 Apex mark에서 근관장을 측정하고 고정한 후 치근단부 4 mm를 삭제하여 현미경상에서 파일 끝부터 주근단공까지의 거리를 측정하였다. 그 결과 Root ZX와 E-Magic Finder 모두, 실험군 100%에서 주근단공과 file tip간의 거리가 임상적 허용범위인 ± 0.5 mm 내에 있었으며 0.5 mark보다 Apex mark에서 더 높은 일관성을 보였다. 주근단공에서 file tip 사이의 거리는 Root ZX의 0.5 mark에서 -0.18 mm, Apex mark에서 -0.07 mm 이고 E-Magic Finder의 0.5 mark에서 -0.25 mm, Apex mark에서 -0.02 mm를 나타내었다. Apex band는 0.04 mm였다. 따라서 Alginate model을 사용한 본 실험조건에서는 Apex mark에서 근관장을 측정한 결과 거의 주근단공과 일치하고 다른 여러 연구에서 주근단공에서 0.5 mm 상방에 근관협착부가 위치한다고 보고하였으므로 임상적인 측면에서 Apex mark에서 측정한 근관장에서 0.5 mm 를 빼서 근관장으로 사용하는 것이 임상적으로 더 좋으리라고 사료된다. The purpose of this study was to evaluate the consistency of two electronic apex locators in vitro model. Materials consisted of fifty two extracted premolars and two electronic apex locators: Root ZX (J.Morita. Osaka, Japan) and E-Magic Finder Deluxe (S-Denti, Cheonan, Korea). After access preparation, the teeth were embedded in a saline-mixed alginate model. Canal lengths of each tooth were measured at "0.5" and "Apex" mark of the apex locators, respectively so that each tooth had two measurements from 0.5 and Apex points. The file was fixed at final measurement using a glass ionomer cement. The apical 4 mm from the apex was exposed to measure the distance from the file tip to the major apical foramen of each tooth. Average distances and standard deviations were used to evaluate the consistency. Results showed that all measurements of both Root ZX and E-Magic Finder located the major foramen the range of ± 0.5 mm level. Both apex locators showed better consistency at Apex mark than at 0.5 mark. The average distance of file tip-major foramen was - 0.18 mm at 0.5 mark and - 0.07 mm at Apex mark in Root ZX, - 0.25 mm at 0.5 mark and - 0.02 mm at Apex mark in E-Magic Finder. Standard deviation was 0.21 at 0.5 mark and 0.12 at Apex mark in Root ZX, 0.12 at 0.5 mark and 0.09 at Apex mark in E-Magic Finder.

하치조신경 및 설신경 손상 평가를 위한 한국인 정상 성인의 하순-이부 및 혀의 감각 조사

이종호,이세영,송승일,이은진,안강민,김성민,명훈,황순정,서병무,최진영,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

In the head and neck area, there are so many sensory nerves, which are sometimes injuried iatrogenically or inadvertently so that involved patients complained of the loss of sensations. In such cases, it is important to judge the degree of injuries and regeneration of nerve for better diagnosis and treatment. Seddon and Sunderland's classification, which is commomly used, is focused on histological change and nerve conduction. As times goes by, it is difficult to access patient's sensory disturbance by this method. Until now, so many methods such as contract threshold, direction, two-point discrimination, pin prick, thermal discrimination and current perception threshold have been introduced for sensory evaluation. However, there hasn't been enough information regarding each methodology nor integrated standard methodology for the measurement. the purpose of this study is to get Korean adult normative sensory values of lower lip,chin and tongue using modified methods of contact thershold, ditection, two point discrimination, pin prick, thermal discrimination and assess degree of regeneration of sensory nerve damage.

RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

성인에서 Haemophilus influenzae 감염증의 임상양상과 미생물학적 특성

이종섭,황병연,정희진,김우주,박승철,이도현,이창규,신종희,황규잠,이영희 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.2

목적 : H. influenzae는 성인에서 흔히 호흡기 감염을 일으키는 균주로, 최근 ampicillin 내성 β-lactamase 생성 균주들이 지속적인 증가를 보이고 있어, 이에 국내 성인에서 H. influenzae에 의한 감염의 임상양상과 분리된 균주들의 미생물학적 특성을 조사하고자 하였다. 방법 : 1998년 3월부터 1999년 9월까지 고려대 구로병원에 내원한 환자중에서 H. influenzae가 분리된 68명을 대상으로 임상양상을 조사하고 항균제 감수성 검사를 시행하였다. 분리된 33주에 대해서 혈청형, 생물형, 세포외막 단백분석을 시행하였고, β-lactamase 생성여부에 따른 감염 환자군간 임상상 및 예후의 차이를 비교하였다. 결과 : 환자의 평균연령은 68세였고, 지역사회 획득감염이 전체의 85%를 차지하였다. 폐렴과 기관지염과 같은 하기도 감염이 전체환자의 75%를 차지하였고 분리 검체 역시 객담이 84%로 가장 많았다. 환자의 71%가 기저질환을 가지고 있었으며, 그 중에 만성 폐쇄성 폐질환, 심혈관 질환, 악성종양, 당뇨병이 대부분이었다. 혈청형으로는 nontypeable 형이 68%로 가장 많았고 b형은 한 예도 없었다. β-lactamase 생성율은 63.2%였고, β-lactamase 양성균주의 항균제 내성율이 음성균주에 비하여 높았으나 β-lactamase 생성 여부에 관계없이 cefotaxime, azithromycin, ciprofloxacin에 대하여 100%의 감수성을 나타내었고 cefaclor, cefuroxime에 대해서는 10% 이하의 낮은 내성율을 보여 이들 항균제들이 치료에 효과적일 것으로 사료된다. 세포외막 단백분석에서는 C형과 D형이 전체의 64%로 가장 많았으며, 혈청형과의 유의한 연관성은 보이지 않았다. 결론 : 성인에서 H. influenzae는 주로 만성 기저질환자에서 급성 호흡기감염 또는 만성 폐쇄성 폐질환의 급성 악화를 유발한다. 기존의 보고에 비해 β-lactamase 양성율이 증가하였고, 이에 따른 항균제 내성율도 증가하여 항균제의 선택에 유의하여야 할 것으로 생각된다. 세포외막 단백분석상 특정한 유형의 균주에 의해 감염이 집중적으로 발생하고 있어 향후 역학 조사의 도구로 이용할 수 있겠다. Purpose : H. influenzae is one of the common causative microorganisms of respiratory tract infections in adults. Recently. β-lactamase producing, ampicillin-resistant H. infiuenzae has become worldwide problem as well as in Korea. We studied to investigate the clinical features and microbiologic characteristics of H. infiuenzae infections in adults. Materials & Methods : From March 1998 to September 1999, 68 patients were enrolled in this study. Clinical features of H. infiuenzae infections were investigated, 68 isolates were subjected to the β-lactamase test, and 33 isolates were used for serotyping. biotyping, antibiotic susceptibility and outer membrane protein (OMP) analysis. Results : Mean age of study patients was 68 years-old and the acquisition rate in community was 85%. Pneumonia and bronchitis were the most common type of infection such as 80%, sputum was the most common clinical specimen for H. mQuenzae isolation. 70% of patients had chronic underlying diseases. Non-typeable strains were 68% and β-lactamase producing rate was 63%. There was no difference in the clinical features and prognosis of H. influenzae infection between the β-lactamase positivie and -negative groups. The susceptibility of cefotaxime, azithromycin, and ciprofloxacin was 100% and that of cefaclor, cefuroxime was under 10% in spite of β-lactamase positivity. In OMP analysis, C and D types were dominant (64%). Conclusion : H. influenzae caused acute respiratory infections in adults with chronic underlying disorders. This study shows higher β-lactamase producing rate and antibiotic-resistance rate than that were reported previously. OMP analysis shows that two major types of strains cause clinical infections intensively.