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황성희 한국융합학회 2022 한국융합학회논문지 Vol.13 No.2
Based on the nutritional component analysis data of the Rural Development Administration, the change in the main nutrients of vegetables for 40 years from 1981 to 2021 was investigated. Nutrients that showed a decreasing tendency during the investigation period in fruit vegetables were calcium, iron, thiamine, riboflavin, and ascorbic acid, etc. In root vegetables, it tended to decrease in calcium, thiamine, riboflavin, niacin, and ascorbic acid, etc. In leaf vegetables, it tended to decrease in iron, niacin, and ascorbic acid, etc. In leaf and stem vegetables, it tended to decrease in energy, phosphorus, iron, thiamine, riboflavin, niacin, and ascorbic acid, etc. In particular, it has been confirmed that vitamin reductions such as thiamine, riboflavin, niacin, and ascorbic acid, which have important meanings in vegetable intake, have occurred in vegetables over the past 40 years.
난소절제 랫드를 이용한 여성갱년기 동물모델에서 변형 사물탕의 효능평가
황성희,박선영,신용국 한국융합학회 2022 한국융합학회논문지 Vol.13 No.4
부작용을 줄인 사물탕을 개발하고자 오미자를 사용한 변형 사물탕을 난소를 절제한 랫드에 경구투여하고 여성갱년기 기능성 효능의 평가지표 변화를 측정하였다. 체중 증가, 자궁과 질의 상대 장기중량, 혈중 지질관련 지표, 혈중 estradiol 의 변화에서 대조군과 비교하여 변형 사물탕이 통계적으로 유의한 개선효과는 나타나지 않았다. 그러나 자궁 조직내 단백질 변화 에서 estrogen receptor alpha 와 beta 의 발현이 증가하는 경향성이 관찰되었고 estrogen receptor 신호전달에 관여하는 것으로 알려진 ERK와 AKT에서 활성화에 기여하는 인산화 ERK의 발현량이 유의한 증가를 나타내었고 인산화 AKT의 발현량 은 유의하진 않지만 증가하는 경향성을 나타내었다. 난소절제로 유도한 여성갱년기 모델에서 시험물질 변형 사물탕을 비교적 적은 용량 투여했음에도 이러한 결과를 나타내는 것은 시험물질인 변형 사물탕에 estrogen receptor를 활성화시키는 능력이 있는 것으로 판단된다. 추후 위장장애가 적으면서 사물탕의 효능을 나타내고자 할 때 유용한 천연물 배합으로 이용이 가능할 것으로 본다. In order to develop samultang with reduced side effects, modified samultang using omija was oral administered to rats with ovarian resection, and changes in evaluation indicators of functional efficacy in women's menopause were measured. In weight gain, relative weight of uterus and vagina, blood lipid-related indicators, and changes in blood estradiol, there was no statistically significant improvement effect in modified Samultang compared to the control group. However, the expression of estrogen receptor alpha and beta in intrauterine tissue tended to increase, and the expression of phosphorylated ERK, which is known to be involved in estrogen receptor signaling, showed a significant increase in activation in ERK and AKT. The expression amount of phosphorylation AKT was not significant, but showed an increasing tendency. Even though the test substance was administered in a relatively small dose, it is judged that the test substance modified Samultang has the ability to activate estrogen receptor. In the future, it is expected that it can be used as a useful natural mixture to show the efficacy of samultang with fewer gastrointestinal disorders.I
Upregulation of S100A9 contributes to the acquired resistance to BRAF inhibitors
황성희,안준호,이미가엘 한국유전학회 2019 Genes & Genomics Vol.41 No.11
Backgrounds Acquired resistance is a significant clinical challenge in targeted therapy of melanomas using BRAF inhibitors. We previously identified that downregulation of miR-92a-1-5p confers acquired resistance to BRAF inhibition using an miRNA array platform. Objective In this study, we investigated the target genes of miR-92a-1-5p and their functional significance in BRAF inhibitor resistance. Methods The miRNA target prediction data were combined with RNA-Seq data to identify possible target genes for miR- 92a-1-5p. Cellular effects of target genes were further examined using siRNA knockdown, WST-1 assay, and immunoblotting analysis. Results We selected S100 calcium-binding protein A9 (S100A9) as a possible target gene for functional validation. S100A9 knockdown abrogated resistance to PLX4720 in A375P/Mdr cells. This result was similar to those described earlier for miR-92a-1-5p, indicating that miR-92a-1-5p inhibits cell viability by targeting S100A9. S100A9 overexpression partially conferred PLX4720 resistance to A375P cells. We also demonstrated that MAPK re-activation does not contribute to the promotion of BRAF inhibitor resistance by S100A9. Conclusion Taken together, our results indicate that S100A9 might be functionally involved in development of resistance to BRAF inhibitors and might be a target for melanoma therapy in the future.
중소도시 중산층 학부모의 자녀 사교육 지원 문화에 관한 연구
황성희 한국교육사회학회 2014 교육사회학연구 Vol.24 No.2
The purpose of this article is to analyze the middle-class parents’ culture of supporting private tutoring of their children. The qualitative method of in-depth interviews was employed to carry out the research. The key informants for the study were twenty-one middle-class mothers in Chuncheon, a local city. The findings of the study can be summarized as follows: The middle-class parents’ culture of supporting private tutoring consists of three parts, center-oriented attitude, implementation, and cultural phenomena. A distinctive attitudinal tendency of the middle-class parents in Chuncheon is their center-oriented attitude, which makes them to set a goal for getting their children admitted into a prestigious university in Seoul. To this end, they support their children with differentiated private tutoring. They keep high-quality tutoring information exclusive to themselves, opt for better programs, and even move their residence to the center of private schooling. As a result, three distinctive phenomena characterize the local city, Chuncheon, namely ‘family co-supporting phenomenon’, ‘tipping phenomenon’, and ‘educational nomad phenomenon’. 이 연구는 중소도시 중산층 학부모들의 자녀 사교육 지원 문화에 관한 것이다. 연구를 수행하기 위하여 수도권 인접 중소도시인 춘천의 중산층 어머니 21명과 심층면담을 실시하였다. 연구결과는 다음과 같다. 자녀 사교육 지원을 위한 학부모 문화에는 교육의식, 실천활동, 그리고 문화적 현상이라는 세 측면이 유기적으로 연계되어 있다. 자녀교육과 관련하여 이 지역 중산층 학부모들에게는 중앙, 특히 서울 지향적인 교육의식이 매우 강하게 흐르고 있으며, 이는 ‘서울 소재 명문대 진학’이라는 자녀교육 목표로 구체화되어 나타난다. 중산층 학부모들은 자녀를 서울의 명문대학에 진학시키기 위하여 차별화된 사교육을 실천한다. 고급정보를 선점하여 일정기간 독점하고, 남보다 질 좋은 사교육을 ‘먼저, 많이’ 선택하고자 한다. 또한 사교육을 효율적으로 관리하기 위하여 전 가족이 함께 역할을 나누고, 핵심교육공간으로의 이동을 재촉한다. 그 결과, 이 지역에는 중산층 가정을 중심으로 ‘가족공동지원 현상’, ‘사교육 쏠림 현상’, ‘교육 유목민 현상’과 같은 문화적 현상들이 나타난다.
황성희,윤광섭 한국산업식품공학회 2008 산업 식품공학 Vol.12 No.4
This study was carried out to investigate the effect of refining effect of crude squid liver oil. The refining processes were consisted of alkaline refining, degumming, bleaching and deodorizing, consequently. L value was increased with duration of refining process but a and b value were decreased. The acid value, the peroxide value, the TBA value, and the conjugated dienoic acid value of refined oil showed decreasing tendency with progress on refining process, and then the refining procedures could be effective process for the oil quality improvement. In ddition, refined oil contained over 50% polyunsaturated fatty acid in total fatty acid. The ratio of saturated fatty acid to polyunsaturated fatty acid was over 2.00, and the composition of major fatty acid did not change during refining process. These results showed that this refining process could improve stability and physicochemical properties of squid liver oil than crude oil.