대장암종에서 암관련 유전자들의 Promoter Methylation과 암종 발생 및 진행과의 관게에 대한 연구

정동준,이정은,민영기,조성우,백무준,양승하,김의한,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The colorectal corcinoma is one of the most common malignant tumor in Korea and its carcinogenic mechanism has not been elucidated exactly yet. So far, the most common carcinogenic mechanism has been known to be multiple genetic changes. But, the CpG island hypermethylation has been revealed to be one of the most important carcinogenic mechanism second to the genetic changes. The epigenetic changes characteristically imprint and transfer to the next generation without changes of nucleotide sequences. When the CpG island of promoter or 5'- exon 1 of a specific gene is methylated by DNA methyltrasferases (DNMTs), the gene expression is inhibited. Carcinogenic mechansisms by promoter methylation has been well known, but the progression of the cancer has not been elucidated yet. The molecular researches on the carcinogenic mechanisms largely depended on the tumor tissue containing various components of cell, due to difficulties in harvesting pure homogeneous tumor cells. Promoter methylatonof the cancer related genes as APc, hMLH2, p16, DAP-K and MGMT in 50 cases of sporadic colorectal carcinoma was studied. The pure normal colorectal epithelial cells, and cancer cells from superficial and deep invasive portion were harvested respectively by laser captured microdissection(LCM), and DNA was extracted from them. The promoter methylation was studied by methylation specific poymerase reaction (MSP) after bisulfite modification of the DNA and the expression of the genes by immunohistochemistry. The results suggests that promoter methylation of the cancer related genes are closely related with the colorectal carcinogenesis and it occurrs from early phase of carcinogenesis, progressing constantly to the late stage. And the promoter methylation of normal epithelial cells increased by age. The p15, known to be the genes related to hematologic malignancy, was closely related to the colorectal carcinoma as well.

삼백초(Saururus Chinensis (Lour.) Bail) 열수추출물의 암세포 저해 효과 및 항산화효소 활성에 미치는 영향

양의주,한정,김현정,이인선 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

삼백초(Saururus Chinensis (Lour.) Bail)열수추출물의 항암활성 검색을 위하여 여러 암세포에 대한 저해효과와 항산화효과를 측정하고 발암물질인 MNNG를 투여한 동물조직내의 항산화 효소 활성변화에 미치는 영향 등을 살펴보았다. 그 결과 삼백초 열수추출물은 특히 혈액암 세포인 U937에 대해 가장 높은 저해 활성을 나타내었고, 또한 Chinese hamster V79 cell을 이용한 H2O2로 유도된 세포독성에 대한 억제효과도 나타내었다. 그리고 삼백초 열수추출물은 MNNG를 투여한 다음 감소된 glutathione S-transferase와 superoxide dismutase의 활성과 glutathion의 함량을 높여주었다. 이상의 결과로 삼백초 열수추출물은 항산화에 관련하여 발암을 예방하리라 사료된다. Chemoprevetion effects of Saururus Chinensis (Lour.) Bail water extracts on several tumor cells, Chinese hamster V79 cells and antioxidant enzymes in Wistar male rats were investigated. The water extracts of Saururus Chinensis (Lour.) Bail showed a higher cytotoxicity effect on the human histiocytic leukemia cells(U937) and protective effects against the cytotoxicity of H2O2. The glutathione content was decreased by MNNG treatment but was increased by adding Saururus Chinensis (Lour.) Bail water extracts. Also the activities of glutathione S-transferase and superoxide dismutase were increased by the treatment of Saururus Chinensis (Lour.) Bail water extracts more than with MNNG alone. These results suggest that the water extracts of Saururus Chinensis (Lour.) Bail inhibit the growth on U937 and has a protective effect on liver and stomach damage by the activities of free radical generating enzymes.

중소규모 사업장 근로자의 직무스트레스로 인한 결근, 조퇴 현황과 한국형직무스트레스 하부요인과의 관련성

김정훈,박신구,김동현,김환철,임종한,이의철,이동훈,이지영 大韓産業醫學會 2009 대한직업환경의학회지 Vol.21 No.2

목적: 중소규모 사업장 근로자들의 직무 스트레스로 인한 결근, 조퇴의 현황과 한국형 직무 스트레스 하부 요인과의 관련성을 알아보고자 하였다. 방법: 중소규모 사업장 83곳 근로자들에 대해 한국형 직무스트레스요인 평가도구-단축형(KOSS-SF)과 사회 일반적 특성, 직업적 특정 및 직무스트레스로 인한 결근이나 조퇴 경험여부 등을 묻는 구조화된 자가 기입식 설문을 실시하였고, 응답한 4,350명에 대해 최종 분석을 시행하였다. 직무스트레스로 인한 결근, 조퇴와 한국형 직무스트레스 하부 요인들과의 관련성을 확인하기 위하여 로지스틱 회귀분석을 시행 하였다. 결과: 직무 스트레스로 인한 결근, 조퇴를 한 근로자는 남자에서 7%(196명), 여자에서 8%(113명) 로 조사되었고 한국형 직무 스트레스 하부요인과의 관련성에서는 남, 여 모두 직무요구, 관계갈등, 조직체계, 보상부적절, 직장문화 영역의 스트레스가 높은 군에서 통계적으로 의미있게 직무스트레스로 인한 결근, 조퇴율이 증가하였다. 결론: 중소규모 사업장 근로자들의 직무 스트레스로 인한 결근, 조퇴와 한국형 직무 스트레스 하부요인과의 관련성은 남자에서는 조직체계 영역이 가장 높았고, 여자는 직장문화 영역에서 가장 높은 관련성을 보였다. Objective: The aim of this study was to examine the actual conditions of absence and early leave due to job stress of 4,350 workers in 83 small and medium scale industries, and its relationship to job stress factors using Korean Occupational Stress Scale-Short Form (KOSS-SF). Methods: A structured self-reported questionnaire was used to assess each respondent's Sociodemographics, job stress, experience of absence and early leave due to job stress. Seven KOSS-SF domains of job stress were used. We estimated the relation of absence and early leave due to job stress and job stress factors using logistic regression analyses. Results: Absence or early leave due to job stress occurred in 7% of male workers and 8% of female workers. Multiple regression analysis revealed a significantly positive association job stress-related absence/early leave and job demand, interpersonal conflict, organizational system, lack of rewards and occupational climate. Conclusions: Absence and early leave due to job stress is significantly attributed to organizational System and lack of reward for men, and occupational climate and organizational system for women.

단백 methylase 억제제 5´-Isobutyl-5´-deoxyadenosine과 sinefungin에 의한 BALB/c mouse 비장세포의 증식과 분화의 억제

최의규,홍정희,한유정,백문기 大韓免疫學會 1995 大韓免疫學會誌 Vol.17 No.3

Whether or not protein methylation can be correlated with cell proliferation and differentiation in BALB/c mouse splenocyte cultures has been investigated by using methylase inhibitors, 5'-Isobutyl-5'- deoxyadenosine(SIBA) and sinefungin. 1) Protein methylase I and III activities were found to be elevated in LPS stimulated splenocytes, and the peak of protein methylase I was correlated with that of S phase fraction while changes in protein methylase III activity followed the changes in S phase fraction. 2) SIBA and sinefungin were proved to be potent competitive inhibitors of the protein methylase 'I and III in vitro with apparent Ki for sinefungin of both enzymes being lower than that for SIBA. 3) The protein methylase III were activated by 2-mercaptoethanol and dithiotreitol, and strongly inhibited by Cue+ in vitro; and the inhibition was almost completely released by dithiotreitol and diethyldithiocarbamate. 4) The extent of polyclonal antibody formation, S phase fraction and protein synthesis after induction by LPS were significantly inhibited in a dose response manner by SIBA and sinefungin with higher potency for the former. 5) Inhibition of cell cycle was also observed in the presence of cyloleucine, an inhibitor in SAM synthesis. 6) Of several enzymes investigated, namely protein methylase III, LDH, yglutamyltranspeptidase, and adenosine deaminase, the- protein methylase III was most markedly increased by LPS and decreased by SIBA and sinefungin than other enzymes. From the above results, it is suggested that SIBA and sinefungin suppress the cell proliferation and differentiation through the inhibition of protein methylation.

法人稅平準化 測定에 관한 硏究

權義晩,韓永熙,鄭柔錫 군산대학교 산업개발연구소 1997 産業開發硏究 Vol.16 No.-

This paper investigates the problems in the measurement regarding corporate income tax smoothing. Past studies generalyy based conclusions on the degree or frequency of apparent tax smoothing behavior identified among sample firms. This investigated two problems in the past studies. The first problem is that much of what appears as tax smoothing may occur "naturally" i.e., without any conscious intent by management to regulate fluctuations in its exemption codes. The second problem lies in the measurement ration of tax smoothing, which may overestimate the fluctuations is corporate income tax. In sum, this study proposed a general methods which taked the possibilities naturally tax smoothing by management and provided the method exactly to measure the smoothness.

5-Fluorouracil 및 mitomycin C가 지라조직에 미치는 영향

고정식,안의태,박경호,김진국,김의한,정영신 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.2

폐의 원발성 미분화 대세포암종의 면역조직화학적 연구

한혜승,서정욱,함의근 대한병리학회 1996 Journal of Pathology and Translational Medicine Vol.30 No.5

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

직무스트레스와 감기와의 관련성

김동현,박신구,김환철,이의철,김정훈,임종한 大韓産業醫學會 2008 대한직업환경의학회지 Vol.20 No.2

목적: 국내외를 통해 직무스트레스와 감기 등과 같은 감염질환과의 관련성에 대한 연구는 많지 않으며, 특히 국내에서의 연구는 거의 전무한 실정이다. 이에 직무스트레스와 감기와의 관련성을 확인하고자 한다. 방법: 일개 대학병원의 산업의학과에서 보건관리대행을 이행하고 있는 83개의 사업장, 근로자 6,699명에 대해 한국형직무스트레스-단축형(KOSS-SF) 설문지를 배부하였고 회수된 4,637명의 설문지 중 부적절한 응답과 다른 호흡기와 관련한 폐질환을 앓고 있는 237명을 제외한 최종분석 근로자 4,400명(65.7%)의 설문자료를 근거로 연구를 시행하였다. 결과: 감기 경험과의 단변량분석에서 유의한 차이를 보인 요인들과 일상생활 스트레스를 보정한 후 시행한 직무스트레스와 감기 경험과의 로지스틱 희귀분석 결과 직무 자율성 결여를 제외한 직무스트레스 하부 영역 전반(직무요구, 관계갈등, 직무불안정, 조직체계, 보상부적절, 직장문화)에 걸쳐 통계적으로 유의한 차이를 보여주었다. 또한 직무스트레스와 감기 경협 첫수와의 관련성을 확인하기위한 다중회귀분석에서도 비슷한 양상을 보였다. 결론: 직무자율성 결여를 제의한 한국형 직무스트레스 요인 전체가 감기와 관련성을 나타내었다. Objectives: To investigate the relationship between job stress and the common cold which is one of the most common infectious disease. Methods: A KOSS (Korean Occupational Stress Scale)-based questionnaire survey was conducted targeting 6,699 workers, of which 4,637 questionnaires were returned and the data for 237 were excluded due to poor response and having pulmonary disease other than common cold. Consequently, 4,400 (65.7%) returned questionnaires were analyzed. Multiple logistic regression analysis and multiple linear regression, adjusted for daily life stress, confounding variables and both, were used to evaluate the relationship between job stress and the common cold. Results: After adjustment for confounding variables and daily life stress, most of the subscales of job stress contributed to an increased risk of common cold. Conclusions: These results indicated that job stress may play a significant role in increasing the risk of the common cold and that further preventive efforts and studies are needed to reduce job stress and address infectious disease caused by job stress among Korean employees.