서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

리튬-필로카르핀 경련모델의 특징 : 행동양상, 뇌파, Fos단백의 발현 및 신경병리학적 변화 Behaviors, Electroencephalography, Fos Expression, and Neuropathologic changes

김영인,이광수,김범생,김용재,천명훈,김명석 대한신경과학회 1996 대한신경과학회지 Vol.14 No.1

The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

항암항생제 Mitomycin C와 DNA의 상호작용

명평근,김춘성,유병태,이천배 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1

Mitomycin C is a clinically used antitumor antibiotic that binds covalently to deoxyribonucleic under reductive or acidic catalysis. We have investigated the confirmation, the determination of thermal melting point(Tm), the inhibition of S1 nuclease and exonuclease I and the transformation of supercoiled closed circular(SCC) PBR 322 DNA on the Mitomycin C-DNA complex. It was identified that Mitomycin C was reduced by 0.001M HC1 (pH<4), 0.01M NaBH_(4) and ascorbic acid. The alkylation of DNA by Mitomycin C was increased Tm; The Tm of control λDNA was 77.25℃ whereas Tm of Mitomycin C-DNA complex was 80℃. It was founded that Mitomycin C-DNA complex inhibited the activity of the S1 nuclease and exonuclease III. The SCC pBR 322 DNA was transformed into more open circular (OC) form by increasing concentration of the Mitomycin C. It is suggested that the interaction of Mitomycin C with DNA effects the conformation of DNA.

퇴행성관절염 노인환자의 우울과 삶의 질 예측요인

전정호,이해정,김명희,신재신 성인간호학회 2003 성인간호학회지 Vol.15 No.4

Purpose: The purpose of this study was t o identify predictors of depression and quality of life among older adults with osteoarthritis. The predictors included in the model were the client's characteristics(age, pain, disease duration, ADLs) , personal resources (hardiness. self-care agency and family support), and depression. Method: 150 subjects who were older than 65 years and had diagnosis of osteoarthritis participated in the study. To answer the research questions. descriptive analysis. Pearson correlation, and hierarchical multiple regression were utilized using SPSS WIN program. Result: Older adults who were younger and had lower levels of pain and dependency on ADLs, and higher levels of self care agency and hardiness reported lower levels of depression(R^(2)=0.517). Older adults who had lower levels of depression, pain, and dependency on ADLs, higher levels of family support and hardiness, and who are younger reported higher levels of quality of life(R^2=0.804). Conclusion: Based on the findings of this study, development of nursing intervention program including pain reduction, enhancing ADL abilities and personal resources (hardiness, family support) can be suggested. Further study is needed t o increase the ability of generalization of the study findings to the broader population.

항암항생재 Mitomycin C와 DNA의 상호작용

명평근,김춘성,유병태,이천배 충남대학교 약학대학 의약품개발연구소 1990 藥學論文集 Vol.6 No.-

Mitomycin C is a clinically used antitumor antibiotic that binds covalently to deoxyribonucleic under reductive or acidic catalysis. We have investigated the confirmation, the determination of thermal melting point(Tm), the inhibition of S1 unclease and exonuclease Ⅲ and the transformation of supercoiled closed circular(SCC) PBR 322 DNA on the Mitomycin C-DNA complex. It was identified that Mitomycin C was reduced by 0.001M HCl pH<4), 0.01M NaBH_4 and ascorbic acid. The alkylation of DNA by Mitomycin C was increased Tm; The Tm of control λDNA was 77.25℃ whereas Tm of Mitomycin C-DNA complex was 80℃. It was founded that Mitomycin C-DNA complex inhibited the activity of the S1 nuclease and exonuclease Ⅲ. The SCC pBR 322 DNA was transformed into more open circular (OC) form by increasing concentration of the Mitomycin C. It is suggested that the interaction of Mitomycin C with DNA effects the conformation of DNA.

자궁경부암의 수술 및 방사선치료 후 국소재발 판정에 대한 MR 및 CT 평가

전하정,이명자,김형석 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.2

The objectives of our study were to analyze the CT and MR findings of local tumor recurrence at vaginal stump in patients of cervical carcinoma treated with hysterectomy and radiotherapy, and to assess the diagnostic value of the MR study. We retrospectively analyzed the CT(n=17) and MR (n=10) findings in 19 patients, who had uterine cervical carcinoma after hysterectomy with radiation therapy (extended-field irradiation and/or brachytherapy). The CT findings of local tumor recurrence were localized mass like lesion(n=5), slightly enhanced infiltrating lesion (n=4) and enhancing mass with central necrosis (n=1). The MR findings wer local iso-signal intensity mass on T1-weighted image and irregular high-signal intensity mass on T2-weighted image. The false positive results were three (two on CT and one on MR), due to inflammation and local fibrosis. On the basis of our study, the CT was the proper screening method for follow up in patients with cervical carcinoma after treatment. But, if any suspicion of tumor recurrence, MR was the useful tool to exclude the local fibrosis and inflammation, and to decide the next studies such as biopsy or observation with MR follow up.

무선인터넷 서비스의 CRM 구현을 위한 평가 요소추출에 관한 연구

全永鎬,孫基爀,金明敎 弘益大學校 科學技術硏究所 2004 科學技術硏究論文集 Vol.15 No.-

According to the realization and commercialization of wireless internet, wireless data communication has been recognized as an important client channel and the CRM (Customer Relationship Management) has emerged as the latest issue for the final increase of income by the improvement of customer relationship. Accordingly, this study is to extract the factors of customer satisfaction from the contents properties provided by wireless internet service and investigate how to realize the ultimate CRM and the influences of each property for customer satisfaction by classification of contents quality, information expression, search, sense touch and system quality. As the result of this study, the contents properties provided by the wireless internet service give the influences on customer satisfaction as the influential factors. In conclusion, the data studied in this paper will be used to promote the customer satisfaction of the wireless internet service providers in their product development and enable the statistical application in evaluation of service quality to increase the sales volume and the related income creation as expected.

새마을工場의 實態分析과 生産性 向上方案 : With Focus on Saemaul Factories of Chunnam District.

金晋鎬,盧春燮,金明洙,李成春 순천대학 새마을연구소 1987 새마을硏究論文集 Vol.3 No.-

The purpose of this paper is to investigate the actual conditions of Sa―emaul Factories and then to suggest their productivity improvement program. The present research ceneteres around Saemaul Factories of Chunnam District, addressing the actual conditions of operation for better productivity. According to the above research, the following will be considered for pr―oductivity improvement program of Saemaul Factories. 1. The designation system of Saemaul Factories should be improved. 2. The impediment factors of operation should be eliminated for better productivity. 3. The educational program should be developed to strengthen members’ sense of productivity. 4. A more efficient assistance system should be sought on the part of government.