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        재래식 및 개량식 된장의 아이소플라본 분포 및 $\beta$-glucosidase 활성 연구

        이승욱,박영우,장판식,이재환,Lee, Seung-Wook,Park, Yong-Woo,Chang, Pahn-Shick,Lee, Jae-Hwan 한국식품과학회 2010 한국식품과학회지 Vol.42 No.2

        숙성기간이 다른 재래식 된장 12종과 개량식 된장 4종의 아이소플라본과 $\beta$-glucosidase 활성도를 비교하였다. 재래식 된장의 전체 아이소플라본 함량(TI)은 $370-723\;{\mu}g/g$이었고, 개량식 된장은 $179-537\;{\mu}g/g$으로 유의적으로 낮은 함량을 보였다. 된장의 주요 아이소플라본은 비배당체 형태였으며 재래식 된장의 경우 42.98-89.96%, 개량식 된장은 35.51-93.48%이었다. 아이소플라본 함량 분포에 대한 주성분 분석 결과 재래식 된장은 개량식 된장과 차별화가 가능하였다. 개량식 된장의 경우 $\beta$-glucoside 형태는 검출되지 않았는데 이는 과도한 열처리를 받았거나 발효에 관여한 미생물의 $\beta$-glucosidase 활성이 높았음을 의미한다. 된장시료에서 $\beta$-glucosidase 활성도는 날콩에 비해 낮았으며 재래식 및 개량식 된장간에 유의적인 차이 및 아이소플라본의 분포와의 상관관계는 명확하지 않았다. Isoflavone distribution and $\beta$-glucosidase activity in 16 commercially available doenjang samples were determined. Twelve of the samples were home-made doenjang (HMD) with a relatively long fermentation period and 4 of the samples were factory-produced doenjang (FPD) from four different manufactures. Total isoflavones (TI) in the HMD ranged from $370-723\;{\mu}g/g$, while those in FPD ranged from $179-537\;{\mu}g/g$. The isoflavone distribution in HMD was different from those in FPD. Generally, the TI in HMD was higher than those in FPD. The major isoflavone was aglycones, which ranged from 42.98 to 89.96% in HMD and from 35.51 to 93.48% in FPD. Isoflavones in the $\beta$-glucoside forms were not detected in tested FPD samples. Principal components analysis (PCA) of the isoflavone profiles showed that HMD were differentiated from FPD. First principal component (PC1) and second principal component (PC2) expressed 43.6 and 22.9% of the data variability, respectively. $\beta$-Glucosidase activity in doenjang was lower than that in raw soybeans. The results of this study can be used to understand the differences in the isoflavone distribution in traditionally manufactured and factory produced doenjang.

      • KCI등재

        임신간격에 관한 분석연구 : 한국농촌 일가 임여성을 대상으로

        이승욱,김정근 한국보건통계학회 1976 한국보건정보통계학회지 Vol.2 No.1

        This is one part of "Community Pregnancy Study" which has been carried out at three Myons of Chunseong Gun area, Kangwon Province over the period covering from May 1973 to April 1976 with financial assistance from UNFPA. In order to increase the sample size the author has included retrospective information for the period from May 1971 through April 1973. Among the three Myons where the study was actually done, one Myon, called Shindong Myon, has been a demonstration area of the Community Health Program by the School of public Health, Seoul National University since 1972. The intensive health care program is being carried out there for the purpose of field training for students, demonstration model for comprehensive health services, and health research activities. In the present analysis, the pregnancy interval is defined as the period between the end of a certain pregnancy and the beginning of next one, and is assumed to be made of two components: Ⅰ) Infecundable period including post·partum amenorrhoea and anovulatory period, Ⅱ) Fecundable period including ovulatory period. Since the pregnancy interval is the elementary basis for the enhancement of MCH, the author attempted to analyze from these data how the types of previous pregnancy terminations affected the following closed pregnancy intervals. This study attempted to compare by Myons Ⅰ) how the family planning programs prolong pregnancy intervals, Ⅱ) the differences among different types of previous pregnancy terminations, and Ⅲ) the impact of pregnancy interval to MCH. The results are summarized as follows. Ⅰ. The number born between 1935-1954 amounted to 655(91.9%) in total, which is reasonable when compared with the distribution of the married women of child-bearing age in general (Table 1). Ⅱ. By the number of women 543(76.4%) experienced one interval, 149(20.7%) two, and others over three (Table 2). Ⅲ. There were 912 cases in total and among them 735(80. 6%) did not practice contraception during the interval (Table 3). Ⅳ.The increase in the number of living children resulted in the lengthening of the pregnancy interval regardless of contraception (Table 4). Ⅴ. The mean of all the intervals was 20.8 months with the use of contraception and 13.4 without it (Table 5). Ⅵ. The pregnancy interval after live birth was 23.3 months with the use of contraception, and 6.3 without it (Table 5). Ⅶ. The interval after fetal death was 13.7 months with contraception and 6. 3 without it. Also the interval after spontaneous abortion was 11. 6 with contraception and 6. 1 without it after induced abortion 14. 5 with contraception and 6. 4 without it (Table 5). Ⅷ. It was observed that as the mothers' age at the beginning & end of interval increased, so did the length of interval regardless of contraception (Table 6). Ⅸ. The regression & correlation analyses were performed on the pregnancy interval by the mothers' age as shown in the end of Chapter Ⅲ. The findings from the results are as follows; Ⅰ. When the contraception is used, the pregnancy interval after live birth is very similar to the one medically recommended. Ⅱ. The longer pregnancy intervals are observed for the higher brackets of mothers' age. Ⅲ. The experience of contraception prolonged the pregnancy interval by 7. 4 months. Ⅳ. It may be desirable to concentrate on the practice of contraception, which is found to be very effective method of family planning, rather than the induced abortions.

      • KCI등재

        역학자료분석에서 Multiple Comparison Procedures에 대한 비교분석 연구

        이승욱,황윤태 한국보건통계학회 1989 보건정보통계학회지 Vol.14 No.1

        Numerous procedures are available for performance of pairwise multiple comparisons among the observed treatment means from designed experiments. Since the magnitudes of the critical values vary among procedures, results obtained from the application of one procedures to a given set of data will often differ from those obtained if another procedure is utilized. In this study, the simulation was performed using hypothetical data for each of 16 sets of treatment means. The results were summarized as follows : 1. In the case of equal variance of each treatment levels, equal or not equal sample size, and the magnitudes of variance regardlessly, Tukey and Scheffe' procedures are more conservative than LSD, Duncan and SNK procedures in specifying the real difference of treatment means. 2. In the case of unequal variance of each treatment levels, LSD, Duncan procedures are more sensitive than SNK, Tukey, Scheffe procedures, especially Scheffe procedure is more conservative than other procedures under the treatments variance in large. 3. By the magnitudes of variance, of all multiple comparison procedures, the group if large variance is more sensitive than small variance group.

      • KCI등재후보
      • KCI등재

        꿀벌부채명나방[Galleria mellonella (L.)] 사육을 위한 경제적 인공사료 개발

        이승욱,이동운,추호렬,Lee, Seung-Wook,Lee, Dong-Woon,Choo, Ho-Yul 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.3

        본 연구는 꿀벌부채명나방의 경제적 사육을 위한 인공사료 개발을 위하여 수행하였다. 가격이 비싼 벌꿀을 대체하여 물엿과 설탕을 이용한 사료별 꿀벌부채명나방의 발육과 발육기간 및 산란수, 성충 수명 등을 조사하였다. 당 종류별 유충의 두폭과 체장은 비슷하였으나 체중은 1/2벌꿀 + 1/2물엿 처리가 255.5 mg으로 가장 무거웠고, 설탕은 144.3 mg으로 가장 가벼웠다. 꿀벌부채명나방 번데기의 체중도 1/2벌꿀+1/2물엿이 196.7 mg으로 가장 무거웠다. 당 종류별 꿀벌부채명나방의 유충 발육기간은 벌꿀이 포함된 사료가 30.9일로 가장 짧았으며 설탕은 36.5일로 가장 길었다. 꿀벌부채명나방의 용화율과 우화율은 79.3% 이상이었다. 모든 사료에서 꿀벌부채명나방의 교미 성충의 수명은 암컷이 $6.3{\sim}8.0$일이었고, 수컷은 $7.9{\sim}11.2$일이었다. 사료별 꿀벌부채명나방 암컷 성충의 산란수는 설탕물 첨가 사료에서 1,269개로 가장 많았다. 당 종류별 인공사료 조성에 대한 비용은 밀기울이 쌀겨보다 10배 정도 많았으며, 벌꿀은 물엿과 설탕의 6배와 13배였다. This research was carried out to develop economical artificial diets of the greater wax moth, Galleria mellonella (L.). Diets were mixed with malt or sugar instead of whole honey for cheaper ones. fecundity, developmental period, pupation rate, emergence rate and adult longevity of G. mellonella depending on diet were examined. The head width and length of larvae were not significantly different among diets tested. However, the weight of larva was the heaviest, 255.5 mg, at 1/2 honey + 1/2 malt diet and lightest, 144.3 mg, at sugar diet. The weight of pupa was also the heaviest at 1/2 honey + 1/2 malt representing 196.7 mg. Larval period was the shortest at honey diet as 30.9 days but the longest at sugar diet as 36.5 days. Pupation and emergence rates were over 79.3% at all diets. The longevity of mated females was ranged from 6.3 to 8.0 days and those of mated males ranged from 7.9 to 11.2 days. The highest number of eggs, 1269, was obtained at sugar diet. Artificial diets replaced by rice bran, malt, and sucrose reduced costs compared with wheat bran plus honey diet. The cost of rice bran was only 10% of wheat bran. Honey was much more expensive than malt and sugar, that is 6 and 13 times, respectively.

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