콜로이드 골드 나노입자의 단백질 수송성 평가법

김미영,노상명,김정목,최한곤,김정애,오유경 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.6

Colloidal gold nanoparticles might be of use as nano scale delivery systems of various therapeutic materials in the firture. Recent studies have reported the feasibility of colloidal gold nanoparticles as gene delivery systems or protein delivery systems. In this study, we aimed to develop a short-step method useful for screening the optimal coating conditions of colloidal gold nanoparticles with proteins. We observed that colloidal gold nanoparticles have properties of changing its unique color when they were exposed to NaCl solution. Taking advantage of the color changing properties of colloidal gold nano-particles, we applied the color testing method of colloidal gold nanoparticles solutions for evaluating the protein coating nature. Using bovine senim albumin as a model protein, we tested the protein coating of colloidal gold nanoparticles via the color change upon NaCl addition. The optimal coating concentration and coating conditions of colloidal gold nanoparticles with bovine serum albumin were fixed using the color testing methods. We suggest that the color testing method might be applied to optimize the coating condition of colloidal gold nanoparticles with other therapeutic proteins.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

골분을 이용한 구리 흡착 첨착

김은정,박정훈,김정섭,유현철,이봉섭,곽명화,우성훈,박승조 東亞大學校 環境問題硏究所 2005 硏究報告 Vol.27 No.1

Spent pig bone was consist of hydroxyapatite. And then we thought that it have a possibility as a adsorbent. The preparation of bone char prepared from spent pig bone in this study. We study on the recycling possibility as a adsorbent is able to adsorb copper ion from their single aqueous solutions. The results are summarized as follows. Bone char that prepared from spent pig bone at 105, 400, 700℃. The adsorption removal efficiency of copper ion onto bone char as adsorbent was above 90% for 15 hours. Increase in the initial pH of the copper ion solution result in an increase in the copper ion uptake per unit weight of the adsorbent. Freundlich isotherm model was found to be applicable for the experiment data of copper ion. The results showed that spent pig bone can be used for the absorption of the copper ion.

국내 다기관에서 조사한 지역사회획득 메티실린내성 황색포도알균의 빈도와 임상적 특성

송진수,최평균,송경호,조재현,김성한,방지환,이창섭,박경화,박경운,신수,최희정,김의석,김동민,이미숙,박완범,김남중,오명돈,김의종,김홍빈,최강원 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

목적 : 최근 전세계적으로 지역사회획득 메티실린내성 황색포도알균(community-associated methicillin-resistant Staphylococcus aureus, CA-MRSA)의 보고가 증가하고 있다. 하지만, 우리나라에서는 CA-MRSA 감염증에 대한 증례보고만 있을 뿐 아직까지 체계적인 연구결과가 없는 실정이다. 저자들은 국내에서 CA-MRSA의 빈도, 감염증의 임상적 양상, 분리된 균주의 항균제내성 양상을 조사하였다. 재료 및 방법 : 2005년 1월부터 2005년 6월까지 7개 병원에서 MRSA가 분리된 환자의 명단을 파악한 후 의무기록지와 건강보험심사평가원의 자료를 검토하였다. 외래나 응급실에서 혹은 입원 후 72시간 이내에 균주가 분리되고 MRSA 획득과 관련된 위험인자가 없는 경우 CA-MRSA로 정의하였으며, 분리된 균주의 임상적 의미에 따라 원인병원체(pathogen), 집락화(colonizer), 미결정(undetermined)으로 분류하였다. Penicillin과 oxacillin을 제외하고 3개 이상의 다른 계열 항균제에 내성이면 다제내성으로 정의하였다. 결과 : 연구기간동안 총 3,251주의 황색포도알균이 분리되었으며, 이 중 MRSA는 1,900주(58.4%)였다. MRSA 가운데 CA-MRSA는 114주(6.0%) 였으며, 이들이 분리된 부위는 귀(62주), 비뇨기계(14주), 피부 및 연부조직(11주), 호흡기계(10주), 혈액(3주) 등이었다. CA-MRSA 균주 가운데 집락균은 22주, 원인병원체는 22주였으며, 나머지 균주에 대해서는 그 임상적 의미를 결정할 수 없었다. 항균제 감수성 검사를 시행한 73균주 중 47주(64.4%)는 다제내성이었다. CA-MRSA 감염증 22예 중 피부 및 연부조직 감염(9예)과 중이염/외이도염(9예)이 가장 흔하였다. 침습적 감염증(invasive infection)은 4명(원발성 균혈증 3예, 감염성 관절염 1예)에서 확인되었지만, CA-MRSA 감염증으로 사망한 환자는 없었다. 결론 : 병원내 감염증에서는 MRSA가 심각한 문제이지만, 아직까지 지역사회 감염증에서 CA-MRSA는 흔하지 않았다. Background : Methicillin-resistant Staphylococcus aureus (MRSA) infection has emerged in patients who do not have the established risk factors. In Korea, little is known about the epidemiology and clinical features of community-associated MRSA (CA-MRSA). Material and Methods : Clinical microbiology laboratory databases of 7 hospitals were reviewed to identify the patients from whom MRSA was isolated during the period of January to July 2005. Only one isolate per patient was enrolled. In order to identify the risk factors of MRSA acquisition, the medical records and the Health Insurance Review Agency databases were reviewed. CA-MRSA was defined as MRSA isolated from patient without established risk factors. We analyzed patient demographics, underlying medical conditions, characteristics of infection, and antimicrobial susceptibility profiles. Results : Of total 3,251 S. aureus isolates, 1900 (58.4%) were MRSAs. Of the MRSA isolates, 114 (6.0%) were CA-MRSA. Of 114 CA-MRSA isolates, 22 (19.3%) were colonizers, 22 (19.3%) were pathogens, and the clinical significance of remaining 70 (61.4%) could not be determined. Median age of the 22 patients with CA-MRSA disease was 47 years. Nine patients had skin and soft tissue infections, 9 ear infections, 3 bacteremia, 1 septic arthritis. Seven patients had underlying medical disease. None died of the CA-MRSA infections. Of the 73 isolates of CA-MRSA, 47 (64.4%) were resistant to more than 3 classes of antibiotics besides β-lactams. Conclusion : Although MRSA is highly prevalent among hospital-associated S. aureus infection, CA-MRSA infections are not common.

항생제 사용과 관련된 Clostridium difficile 감염증

김홍빈,김남중,최희정,오명돈,김의종,최강원 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.1

목적 : Clostridium difficile 감염증은 중요한 원내 감염중의 하나로 최근 들어 항균제 사용의 증가와 함께 그 빈도가 늘어나고 있어 Clostridium difficile 감염의 빈도 및 임상양상을 알기 위해 본 연구를 시행하였다. 방법 : 102명의 정상 성인으로부터 대변에서 Clostridium difficile 배양을 시행하였으며, 92년 3월부터 93년 9월까지 서울대학교 병원에 입원한 환자중 대변배양 검사에서 Clostridium difficile이 배양된 61명의 환자를 대상으로 병록지를 토대로 조사하였다. 결과 : 정상 성인 중 3%에서 Clostridium difficile이 분리되었다. 대상환자의 기저 질환은 악성고형종양(29%), 혈액종양(6.5%), 뇌혈관 질환(6.5%), 간경변(4.9%) 등의 순이었으며, 대상 환자의 75%에서 균이 분리되기 6주 이내에 항균제가 사용되었다. 사용된 항균제는 aminoglycoside(60.8%), 1세대 cephalosporin(56.5%), 3 세대 cephalosporin(43.4%), ampicillin(15.2%), metronidazole(15.2%), clindamycin(8.6%)의 순이었다. 흔한 증상은 설사(77%), 복통(53.8%), 오심(37%), 빈맥(25%) 등이었며, 혈액 검사상 백혈구증가증은 21.6%, 저알부민혈증은 3.03%에서 관찰되었다. 44명의 환자중 24명의 환자가 대증적 치료 후 호전되었고, vancomycin이나 metronidazole을 투여받은 17명의 환자중 15명의 환자가 증상의 호전을 보였다. 4예가 사망하였으며, 3예(4.9%)는 재발하였다. 결론 : Clostridium difficile 장염은 항균제 사용시 흔히 발생하는 질환이다. 정상 성인의 3%에서 Clostridium difficile이 분리되었다. Objective : Clostridium difficile gastrointestinal disease is an important nosocomial infection and is associated with antibiotic use. This study evaluated the incidence and clinical characteristics of Clostridium difficile infections. Methods : Stool specimens from 102 normal adults were cultured for Clostridium difficile. Sixty-one patients with Clostridium difficile isolated from stool specimens were reviewed retrospectively. The patients were seen at the Seoul National University Hospital during the period from Mar., 1592 to Sep., 1993. Results : Clostridium difficile was isolated in 3% (3/102) of normal adult Population. Underlying diseases of the patients included solid tumor(29%), hematologic malignancy(6.5%), corebrovascular disease(6.5%), and liver cirrhosis(4.9%). Antibiotics associated with Clostridium difficile infections included aminoglycosides (60.8%), first generation cephalosporin(56.5%), third generation cephalosporin(43.4%), ampicillin(15.2%), metronidazole(15.2%), and clindamycin(8.6%). In 3/4 of the cases, these antibiotics had beer) administered within 6 weeks before Clostridium difficile isolated. Associated symptoms included diarrhea(77%), abdominal pain(53.8%), nausea(37%), fever(37.7%), and tachycardia(25%). Leukocytosis and hypealbuminemia were observed in 21.6% and 30.3%. Out of 44 cases received symptomatic treatment, 54% (24/44) improved. Of 17 cases treated with metronidazole or vancomycin, 88% (15/17) improved. Four cases expired and 3 cases(4.9%)were relapsed. Conclusion : Clostridium difficile gastroenteritis is frequently associated with antibiotic treatment. Three percent of normal adult population had Clostridium difficile.

느릅나무 근피의 화학조성분 및 항균성 물질(Ⅱ) : 항균성 물질의 단리 및 화학구조 Isolation and Chemical Structure of Antibacterial Compound

김창순,이중명,최장옥,박성배,엄태진 한국목재공학회 2003 목재공학 Vol.31 No.1

느릅나무 근피의 메탄올 추출물을 분획하여 항균성을 측정한 결과 항균성을 측정한 결과 항균력이 가장 우수한 수용성 분획을 silicagel column chromatograpy 및 TLC에 의해 분리·정제한 다음 정제 화합물을 UV, IR, ^H-NMR, ^13C-NMR를 사용하여 구조를 동정하고 그 화합물의 항균력을 검정하였다. 수용성 분획물로부터 두 종류의 정제 화합물을 단리하였다. 기기분석 결과, 화합물 B는 Catechin rhamnoside로 확인되었다. 단리된 정제화합물은 Staphylococcus aureus 및 Salmonella typhimurium에 대하여 항균활성을 나타내었다. The methanol extractives from root bark of Ulmus davidiana var japonica nakai were fractionated with n-hexane, ethyl ether, ethyl acetate and waster, the water soluble fraction was also fractionated with silicagel column chromatograhy. The chemical structure of purifided compounds were indentified with UV, IR, ^1H-NMR and ^13C-NMR spectra and the antibacterial activities also were investigated. Two different antibacterial compounds(compound A and B) were fractionated with silcagel chromatography and TLC. Compounds B was identified as a catechin rehmnoside. The both of compounds had antibacterial activity on Stapbylococcus aureus and Salmonella typbimurium.

초산모의 분만유형과 모성관련지식에 관한 연구

김혜란,노진아,손은정,이명아,김증임 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Purpose: This study was to know primipara's general characteristics, delivery type, delivery characteristics, maternity related knowledge level, and relation of delivery type and maternity related knowledge level. Method: This is a survey. Subjects were sixty primiparas who had admitted to a pediatric outpatient clinic at general hospital in Seoul. Subject's age was ranged from 15 to 44 and it was lapsed 10 weeks after delivery of their first baby. Maternity related knowledge level was measured by "primipara's maternity related knowledge level". Data was nalyzed using frequency. percentage, mean, standard deviation, ANOVA, t-test by SPSS 11.0. Result: Relation of delivery type and maternity related knowledge in primipary did not show a statistically significant. But the relation of natural delivery and maternity related knowledge in primipara was a littlehig than that of caesarean section. Conclusion: There is no significant diference of maternity related knowledge between natural delivery and caesarean section. We suggest for randomized clinical trials with a larger sample size.

다이빙 조사에 의한 가을철 가거도 연안의 어류상

명정구,조선형,박정호,백상규,김종만,강필선 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.3

1. 2002년 10월 16∼17일에 스쿠버다이빙, 수중 촬영을 통하여 전라남도 신안군 가거도 주변 가을철 연안의 어류상을 조사한 결과 총 5목 23과 40종이 확인되었고, 지금까지 기재 된 어종 이외에 18종이 추가로 확인되었다. 2. 어종 구성으로 보면 양볼락과가 7종으로 가장 많았으며, 그 다음으로 둑중개과가 4종, 자리돔과가 3종 순이었다. 3. 가거도 주변에서 지금까지 확인된 어종들은 온대성 어종이 대부분이었지만, 그 외에 노랑자리돔, 청황베도라치 및 파랑돔 등 난류성 또는 아열대 어종이 확인되어 제주도나 독도와 마찬가지로 대마난류의 영향도 받고 있음을 나타내었다. The fish fauna in the coastal waters of Gageodo Island, South-west Sea, was investigated by SCUBA diving in October 16 and 17, 2002. The fishes observed were classified into 40 species in 23 families. In Autumn, subtropical fishes in such as families as Pomacentridae, Pomacanthidae and Apogonidae, represented 12.5% of the total, while eurythermal species represented 72.5%.

정지형 자전거 운동 훈련이 건강한 여성의 체구성,심폐기능 및 유연성에 미치는 효과

김동옥,최정숙,안혜영,민혜숙,이경숙,박연환,송미령,최명애,최정안,김매자 서울대학교 간호대학 간호과학연구소 1999 간호학 논문집 Vol.13 No.1

The purpose of this study was to determine the changes in body composition, cardiopulmonary function, and flexibility following 8 weeks' bicycle ergometer exercise training. The subjects of this research consisted of healthy adult women between 30 and 40 years of age. Initial intensity of bicycle ergometer exercise was based on the target heart rate equivalent to 55% of maximal oxygen uptake, and intensity of the exercise increased by 5% every 2 weeks. Body composition, cardiopulmonary function at rest and during maximal exercise, as well as flexibility were determined before and after 8 weeks of bicycle ergometer exercise training. Masimal exercise was performed on the treadmill according to Bruce Protocol. The results obtained were as follows; 1. There were o changes in body weight, percent body fat, fat body weight, and lean body weight as well. The ventilation volume for 1 minute, respiratory quotient and expired CO₂ volume have not changed significantly after the training, either. 2. As a result of training, resting systolic and diastolic blood pressure decreased significantly(p<.05) 3. Maximal heart rate, maximal oxygen consumption and maximal running time increased significantly after the training(p<.05) 4. Pelvic flexibility increased slightly after the training, however, it did not reach the statistical significance. Trunk flexion forward increased significantly after the training(p<.05). From these results, it may be concluded that 8 weeks bicycle ergometr training improve the cardiopulmonary function and flexibility in healthy adult women between 30-40 years of age.

황 첨착 활성탄에 의한 구리제거

김영환,박정훈,김정섭,유현철,이봉섭,곽명화,우성훈,박승조 東亞大學校 環境問題硏究所 2005 硏究報告 Vol.27 No.1

This study was carried out to remove copper ion in case of activated carbon impregnated sulphur(ACS) dose and different pH using activated carbon impregnated sulphur that prepared from activated carbon at sulphur dioxide atmosphere. Removal copper ion concentration was increased as increasing the ACS dose when copper ion concentration was 10 mg/L. Optimum dose of ACS 13.3 g ACS/L and maximum removal efficiency was 93%. The results obtained from adsorption experiment in the condition of ACS optimum dose when the pH was 3, 4, 5 respectively was pH 5 was excellent.