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      • SCOPUSKCI등재

        면역학적인 방법에 의한 한우와 유우의 요네병 발생조사

        김종만,안종삼,우승룡,조동희,조윤상,박정문,윤용덕,장국현,Kim, Jong-man,Ahn, Jong-sam,Woo, Seung-roung,Jo, Dong-hee,Jo, Yun-sang,Park, Jeung-moon,Yoon, Yong-dhuk,Chang, Guk-hyun 대한수의학회 1994 대한수의학회지 Vol.34 No.1

        A immunological survey of paratuberculosis in dairy and Korean native cattles was conducted by enzyme linked immunosorbent assay(ELISA), complement fixation test(CFT), agar gel immunodiffusion test (AGID) and intradermal skin test(ID). Over all prevalence of pararuberculosis in cattles was 6.7%(109/1633) by ID, 7.5(205/2719) by AGID, 9.3% (245/2641) by CFT and 13.4%(363/2719) by ELISA. Prevalence in dairy cattle was higher than that of Korean native cattle. Of 70 ELISA-positive cattle, 23(28.6%) and 48(68.6%) cattles were classified as positive in the AGID and positive or suspect in CFT, respectively. Of 92 ELISA-suspect cattle, 32(34.9%) and 48(52.2%) cattles were classified as AGID-positive and CFT-positive or suspect, respectively. It was concluded that paratuberculosis is widespread in cattle of Korea.

      • SCOPUSKCI등재

        Levamisole, selenium 및 tocopherol이 한국재래산양의 혈중 호중구 및 복강 대식구의 기능에 미치는 영향

        김종만,마점술,전윤성,Kim, Jong-man,Mah, Jum-sool,Jeon, Yun-seong 대한수의학회 1991 대한수의학회지 Vol.31 No.3

        In this study, the immunomodulating effects of levamisole, selenium and tocopherol on blood neutrophils and peritoneal macrophages of goat were evaluated in vitro and in vivo. The functions of blood neutrophils and peritoneal macrophages were assayed by random and direct migration, phagocytosis of S aureus, production of superoxide and hydrogen peroxide. The results obtained were summarized as follows: In vitro trials 1. Levamisole treatment enhanced the random and direct migration of goat blood neutrophils when compared with untreated cell, and a significant (p<0.01) enhancement was noticed at the concentration of $100{\mu}g$ for direct migration and $50{\mu}g{\sim}1,000{\mu}g\;per\;ml$ of culture medium for random migration. There was no influence of selenium and tocopherol on random and direct migration of neutrophil at all of treatment concentration. 2. Neutrophils produced higher levels of superoxide by lcvamisole treatment at the concentration of $100{\mu}g$ and by selenium treatment at the concentration of $1.0{\mu}g$, but the production of hydrogen peroxide was not increased. Tocopherol had no effect on the production of antimicrobicidal oxygen metabolites of neutrophils at various concentrations. 3. No differences of phagocytic activity were observed when neutrophils were treated with three substances. In vivo trials 1. Blood neutrophils of goats orally administered levaraisole showed significantly (p<0.05) higher random migration from 2 to 24 hours after feeding (2.5mg/kg of body weight). Augmentation of random migration of neutrophil from goats orally administered selenium-tocopherol mixture (selenium $100{\mu}g$-tocopherol 200IU/head/day) was observed at 10 days and the significant (p<0.05) increase was shown from 30 days after feeding and continued throughout the feeding periods. 2. There was no effect on phagocytic activity and production of antimicrobicidal oxygen metabolites of neutrophils from goats administered levamisole or selenium-tocopherol mixture. 3. Random migration, production of superoxide and hydrogen peroxide and S aureus phagocytic activity of peritoneal macrophages of goats administered 300ml of levamisole-thioglycollatc medium mixture $(2.5{\mu}g/ml)$ into peritoneal cavity increased significantly (p<0.01 or p<0.05) when compared with those of goats administered thioglycollate medium alone.

      • KCI등재

        C4I 시스템 사용의 영향 요인에 관한 연구: 구조모형의 매개변수의 관점에서

        김종만,김인재 한국경영정보학회 2009 Asia Pacific Journal of Information Systems Vol.19 No.2

        The general aspects for the future warfare shows that the concept of firepower and maneuver centric warfare has been replacing with that of information and knowledge centric warfare. Thus, some developed countries are now trying to establish the information systems to perform intelligent warfare and innovate defense operations.The C4I(Command, Control, Communication, Computers and Intelligence for the Warrior) systems make it possible to do modern and systematic war operations. The basic idea of this study is to investigate how TAM(Technology Acceptance Model) can explain the acceptance behavior in military organizations. Because TAM is inadequate in explaining the acceptance processes forcomplex technologies and strict organizations, a revised research model based upon TAM was developed in order to assess the usage of the C4I system.The purpose of this study is to investigate factors affecting the usage of C4I in the Korean Army. The research model, based upon TAM, was extended through a belief construct such as self-efficacy as one of mediating variables. The self-efficacy has been used as a mediating variable for technology acceptance, and the variable was included in the research model. The external variables were selected on the basis of previous research. The external variables can be classified into following: 1) technological, 2) organizational, and 3) environmental factors on the basis of TOE(Technology-Organization-Environment) framework. The tech-nological factor includes the information quality and the task-technology fitness. The organizational factor includes the influence of senior colleagues. The environmental factor includes the education/train data. The external variables are considered very important for explaining the behavior patterns of information technology or systems.A structured questionnaire was developed and administrated to those who were using the C4I system. Total 329 data were used for statistical data analyses. A confirmatory factor analysis and structured equation model were used as main statistical methods. Model fitness indexes for measurement and structured models were verified before all 18 hypotheses were tested.This study shows that the perceived usefulness and the self-efficacy played their roles more than the per-ceived ease of use did in TAM. In military organizations, the perceived usefulness showed its mediating effects between external variables and dependent variable, but the perceived ease of use did not. These results imply that the perceived usefulness can explain the acceptance processes better than the perceived ease of use in the army. The self -efficacy was also used as one of the three mediating variables, and showed its mediating effects in explaining the acceptance processes. Such results also show that the self-efficacy can be selected as one possible belief construct in TAM.The perceived usefulness was influenced by such factors as senior colleagues, the information quality, and the task-technology fitness. The self-efficacy was affected by education/train and task-technology fitness. The actual usage of C4I was influenced not by the perceived ease of use but by the perceived usefulness and selfefficacy. This study suggests the followings: (1) An extended TAM can be applied to such strict organizations as the army; (2) Three mediation variables are included in the research model and tested at real situations; and (3) Several other implications are discussed.

      • 자돈의 병원성 대장균증과 난황항체 활용

        김종만 대한수의사회 1998 대한수의사회지 Vol.34 No.8

        병원성대장균에 의한 자돈의 대장균증 중 대표적인 것이 신생 및 포유자돈에서 주로 발생하는 설사병과 이유자돈의 부종병을 들 수 있다. 이러한 자돈의 대장균증은 돼지유행성설사병(PED)이나 전염성위장병(TGE)처럼 폭발적인 발생을 하지는 않지만 지역이나 계절에 관계없이 광범위하게 지속적으로 발생하고 있기 때문에 전체적인 피해는 오히려 폐사율은 높으나 주기적이고 산발적인 발생을 하는 바이러스성 설사병보다도 많을 것으로 추정된다. 이러한 자돈의 대장균설사증 대책으로 몇종의 백신이 사용되고 있으나 완벽한 장관면역을 형성시켜주는 예방약은 아직 세계적으로도 개발되어 있지 않은 실정이고, 치료대책으로 사용하고 있는 항생제도 내성균 확산으로 치료제 사용이 한계에 이르고 있다. 더욱이 부종병의 경우 치료 및 예방대책이 없어 양돈농가로서는 속수무책인 실정이다. 수의과학연구소에서는 이러한 문제점을 해결하기 위하여 대장균증을 동시에 치료, 예방할 수 있는 병원성대장균에 대한 난황항체를 개발하여 실험실 및 야외적용시험을 실시한 결과 대장균설사증은 물론 부종병치료에도 탁월한 효과를 나타내었기에 소개드리고자 한다.

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