Benzyl Isothiocyanate Inhibits Prostate Cancer Development in the Transgenic Adenocarcinoma Mouse Prostate (TRAMP) Model, Which Is Associated with the Induction of Cell Cycle G1 Arrest

Cho, Han Jin,Lim, Do Young,Kwon, Gyoo Taik,Kim, Ji Hee,Huang, Zunnan,Song, Hyerim,Oh, Yoon Sin,Kang, Young-Hee,Lee, Ki Won,Dong, Zigang,Park, Jung Han Yoon MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.2

<P>Benzyl isothiocyanate (BITC) is a hydrolysis product of glucotropaeolin, a compound found in cruciferous vegetables, and has been shown to have anti-tumor properties. In the present study, we investigated whether BITC inhibits the development of prostate cancer in the transgenic adenocarcinoma mouse prostate (TRAMP) mice. Five-week old, male TRAMP mice and their nontransgenic littermates were gavage-fed with 0, 5, or 10 mg/kg of BITC every day for 19 weeks. The weight of the genitourinary tract increased markedly in TRAMP mice and this increase was suppressed significantly by BITC feeding. H and E staining of the dorsolateral lobes of the prostate demonstrated that well-differentiated carcinoma (WDC) was a predominant feature in the TRAMP mice. The number of lobes with WDC was reduced by BITC feeding while that of lobes with prostatic intraepithelial neoplasia was increased. BITC feeding reduced the number of cells expressing Ki67 (a proliferation marker), cyclin A, cyclin D1, and cyclin-dependent kinase (CDK)2 in the prostatic tissue. <I>In vitro</I> cell culture results revealed that BITC decreased DNA synthesis, as well as CDK2 and CDK4 activity in TRAMP-C2 mouse prostate cancer cells. These results indicate that inhibition of cell cycle progression contributes to the inhibition of prostate cancer development in TRAMP mice treated with BITC.</P>

Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

4-Aminosalicylic acid 誘導體의 合成硏究 (Ⅵ) : P.A.S. di 치환 amide의 Alkoxy 誘導體合成 Alkoxy derivatives of disubstituted p-aminosolicylamide

林中基,李漢九,元正喜 成均館大學校 1981 論文集 Vol.29 No.-

4-Acetaminosalicyldialkylamidc was synthesized by amidation of N.O.-diacetyl-4-aminosalicyl chloride with N.N.-dimethylamine or N.N-diethylamine. 2-Alkoxy-4-acetaminodialkyl-benzamide was synthesized by alkylation of 4-acetamino-salicyl dialkylamide with alkyl halides or dimethyl sulfate.

화상을 입힌 생쥐의 흉선과 비장 임파구아형과 조직학적 소견의 변화

한기택,안상태,강윤섭,임 풍 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.4

Due to various documented abnormalities of cell mediated immunity, sepsis still remains the major cause of morbidity and mortality after initial survival from extensive thermal burn. However, the exact mechanisms of immunosuppression after extensive thermal burn remains uncertain. For th observation of immune state, changes of lymphocyte subpopulation have been usually measured in lymphocyte samples from peripheral blood by many reports, but it is only a small fraction of the total lymphocyte pool in the body, and may not present valid picture of the immunological state of the individual. Therefore, analysis of lymphocyte phenotype in thymus and spleen is thought to be necessary in understanding more exact mechanism of immunological changes in extensive thermal burn. The aim of this study is to evaluate the changes of immunocompetency by observing the changes of subpopulation of spleen and thymus lymphocyte using monoclolnal antibodies and changes of tissue with light microscope in burned mice. Cell populations were isolated from spleen and thymus of control and burned mice on the 1st, 3rd, 5th, 7th, 14th and 21st postburn days(PBD). The cell populations were measured with monoclonal antibody by flow cytometry. The results were as follows: 1.In thymus, Thy 1.2+, L3T4+ and LYt2+ cells decreased markedly on the 3rd PBD. Thy 1.2+ and L3T4+ cells decreased also on 5th, 7th, 14th PBD and returned to control level on 21st PBD, but Lyt2+ cells increased more significantly than control after 3rd PBD and returned to normal on 21st PBD. Qa2+ cells revealed most significant reduction on 7th PBD. 2.In spleen, Thy 1.2+, L3T4+ and Ia+ cells increased on 3rd PBD and then decreased on 7th and 14th PBD. Lyt2+ cells increased in all the burned groups. Qa2+ cells increased on 3rd, 5th PBD and returned to normal level. L3T4+ cell/Lyt2+ cell ratio revealed signicant reduction, but they recovered by the 21st PBD. 3.In histopathologic findings of the spleen & thymus, the all cell subpoulations decreased at 1st PBD, but more increased than that of group of 3rd PBD. And then they revealed normal architecture after 5th PBD.

WDHA(Watery diarrhea, Hypokalemia, Achlorhydria) syndrome 1예

박기룡,김현영,김지혜,임영찬,최영식,박요한,최경현,이재화,장희경 고신대학교 의학부 2003 高神大學校 醫學部 論文集 Vol.18 No.1

저자들은 부갑상선절제술 이후에도 교정되지 않는 고칼슘혈증과 계속된 수양성 설사로 인한 하지마비 및 전신쇠약을 주소로 내원한 70세 남자환자에서 일반 화학검사, 호르몬 건사, 방사선 검사 등으로 VIPoma와 동반된 MEN Ⅰ으로 진단하여 외과적 절제술 후 수양성설사, 저칼슘혈증 및 고칼슘혈증이 호전된 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Multiple endocrine neoplasia I (MEN Ⅰ) is a genetic disorder that consists of neoplasia of neuroendocrine type in the parathyroid glands, in the islets of Langerhans in the pancreas, and in the anterior pituitary gland. Primary hyperparathyroidism is most common feature of the MEN I and occurs is approximately 95% of patients. Pancreatic islet cell tumors occur in 40% of MEN I patients. Most of these tumors produce excessive amounts of hormone, such as gastrin, insulin, glucagon and vasoactive intestinal polypeptide (VIP). VIP-producing pancreatic tumors (VIPoma) associated with MEN I are rare and there was only one case report in Korea so far. Recently we experienced a case of MEN I, especially VIPoma presented in a 70 year old man. He got subtotal parathyroidectomy for hyperparathyroidism 5 months ago, but his symptoms of watery diarrhea, severe general weakness and paralysis of lower limbs were not improved and hypercalcemia, hypokalemia were persisted. On computerized tomography scan of abdomen, demonstrated a mass of 3㎝ in diameter at the tail of pancreas. Serum level of VIP hormone was 250pg/㎖ (normal range : 0∼23pg/㎖). After partial pancreatectomy, his symptoms were improved dramatically and biochemical parameters such as, serum potassium, calcium, and VIP returned to the normal ranges.

한라산 혼파목초지에 있어서 억새 (Miscanthus sinensis)의 계절적 연차적 생육변화

조남기,김한림,고영희 제주대학교 1984 논문집 Vol.17 No.-

This study was conducted to examine the yearlv and seasonal changes of Miscanthus sinensis on the improved pasture of the mountain districts around Mt. Halla in Cheju Island. For this study, the researcher had investigated for six years from 1977 to 1982 the improved pasture which had been used for grazing from 1977 to 1982. The pasture was reclaimed from native grassland. The mixed seeds of 17 kgs' Dacrylis glomerata, 7 kgs' Festuca arundinacea, 2 kgs' Lolium multiflorum and 2 kgs' Trfolium repens were sowed per 1 ha. The results of this study are the following; 1. The seasonal changes of Miscanthus sinensis: its length, weight, density and coverage are heighest in Autumn, and are lowest in Spring every year. 2. The yearly change in the length of Miscanthus sinensis: its length is gradually increased from 46.19cm in the first year after the reclaimation to 58.81 cm in the 6th year. This change can be represented by the regression equation, y=1.10x^(2)+l.96x +44.02. 3. The yearly change in its density: its density is rapidly increased from 0.06% in the first year to 0.72% in the sixth year. The results of this change can be obtained with the regreuion equation, y=0.04x^(2)-0.11x + 0.07. 4. The yearly change in the coverage: its coverage is increased year by year from 0.34% in the first year to 5.67% in the sixth year. This change can be calculated with regression equation, y=0.18x^(2) + 0.12x-0.42. 5. The yearly change in its weight: the weight is also increased year after year from 7.98 kg in the first year after reclaimation to 130.04kg in the sixth year. This change can be calculated with the regression equation of y=5.88x^(2)-15.27x + 15.24.

다수의 불규칙 공동을 갖는 광주의 안정성에 관한 수치해석

민형기,임한욱 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.A

A room and pillar mining method has been adopting at the Jeungsun limestone mine . To check stability of pillar with multiple and irregular openings, the size, shape and spacing of nb pillar were first designed using some empirical suggestions. The Finite Difference Method(FDM)was used to analyze the pillar stability. Twelve different cases with the variation of K(horizontal/vertical stress)values, different height and different spacing of pillar were used in this study. Finally Mohr-Coulomb criterion was adopted to calculate the safety factors. Horizontal and vertical displacement. maximum and minimum principal stresses, range of plastic zone and safety factors were calculated at each case. As a result of analysis, the size of one block is 160m long, 70m wide, 40m high with 20m wide rib pillar and 20m square column pillar. The overall recovery at this case can be estimated about 40%.

上東地區 重石鑛의 破壞特性에 關한 硏究

金沂柱,李喜寶,金永錫,林漢旭 全北大學校 1980 論文集 Vol.22 No.-

A Study on failure strength of rock under uniaxial and triaxial compression tests was carried out for underground structural design in domestic and foreign countries recently. An experimental study was made for the core specimens of SANG-DONG tungsten mine. The results are as follows; 1. Failure strength was increasing gradually with increase of confining pressure. 2. Longitudinal and lateral strain increased in proportion to confining pressure under triaxial compression tests. 3. Stress-strain curves showed straight line to 62% of failure strength. 4. Effect of failure strength about porosity was 3.0% on the average and it represented lower value comparatively. 5. The values of triaxial compressive strength were distributed between 345 and 3082㎏/㎠, the mean value of which was 1063㎏/㎠. 6. The mean value of modulus of longitudinal elasticity(E) was 3.6X 10^5㎏/㎠ and poisson's ratio was 0.35.

濟州道 改良草地에 있어서 띠(Imperata cylindrica)의 季節的 年次的 變化

趙南棋,金翰琳,宋昌吉 제주대학교 1983 논문집 Vol.16 No.-

本 硏究는 濟州道 中山間地帶의 自然草地(Imperata cylindrica/Zoysia japonica type)를 開墾한 後 1 ha當 Dactylis glomerata 17kg, Festuca arundinacea 7kg, Lolium multiflorum 2kg, Trifolium repens 2kg의 導入牧草를 混播(28kg)하여 家畜放牧에 利用되고 있는(1977∼1982年) 改良草地를 對象으로하여, 띠(Imperata cylindrica)植生의 季節的ㆍ年次的變化過程을 調査한 結果를 要約하면 다음과 같다. 1. 每年 季節에 따르는 草長ㆍ收量ㆍ密度ㆍ被度 等 植生의 季節的 變化에 있어서는 秋期에 가장 優勢하였고, 春期에는 低調하게 나타났다. 2. 改良後 年度別 草長의 變化는 1年草地에서 34.26cm이었으나 점차 增加되어 6年草地에서는 49.35cm이었는데, 이 變化狀態의 回歸方程式은 y=-0.13x²+4.41x+28.50이었다. 3. 年數의 經過에 따르는 密度의 變化는 1年草地에서 0.76%였던 것이 年數가 지남에 따라 급격히 增加되어 6年草地에 이르러서는 32.97%로 나타나고 있는데, 이 變化를 y=0.10x²+0.32x-2.30의 回歸方程式으로 表示할 수 있었다. 4. 草地造成 以後 年次別 被度의 變化는 1年草地에서 1.05%, 2年 1.38%, 3年 2.46%, 4年 17.11%, 5年 27.56%, 6年草地에서 36.66%로 每年 增加(y=1.40x²-2.36x+1.39)되었다. 5. 收量의 年次的 變化는 1年草地에서 1.95kg이었으나 年數가 지남에 따라 점차 增加되어 6年草地에서는 1,107.35kg으로 나타났는데, 이 變化狀態를 y=29.11x²+53.52x-154.67로 表示할 수 있었다. This study was conducted to examine the yearly and seasonal changes of Imperata cylindrica on the improved pasture of the mountain districts around Mt. Halla in Cheju Island. For this study, the researcher had investigated for six years from 1977 to 1982 the improved pasture which had been used for grazing from 1977 to 1982. The pasture was reclaimed from native grassland. The mixed seeds of 17kgs' Datylis glomerata, 7kgs' Festuca arundinacea, 2 kgs' Lolium multiflorum and 2kgs' Trifolium repens were sowed per 1 ha. The results of this study are the following : 1. The seasonal changes of Imperata cylindrica : its length, weight, density and coverage are heighest in Autumn, and are lowest in Spring every year. 2. The yearly change in the length of Imperata cylindrica : its length is gradually increased from 34.26 cm in the first year after the reclaimation to 49.35 cm in the 6th year. This change can be represented by the regression equation, Y=-0.13x²+4.41x+28.50. 3. The yearly change in its density : its density is rapidly increased from 0.73% in the first year to 32.97% in the sixth year. The results of this change can be obtained with the regression equation, Y=0.10x²+0.32x-2.30. 4. The yearly change in its coverage : its coverage is increased year by year (1.05% in the 1st year, 1.38% in the 2nd year, 2.46% in the 3rd year, 27.56% in the 5th year, and 36.66% in the 6th year). This change can be calculated with regression equation, Y=1.40x²-2.36x+1.39. 5. The yearly change in its weight : the weight is also increased year after year from 1.95 kg in the first year after reclaimation to 1107.35kg in the sixth year. This change can be calculated with the regression equation of y=29.11x²+53.52x-154.67.