중증 허혈성 지체질환 환자에서 시행된 vascular endothelial growth factor의 혈관신생 유전자치료 1예

김현중,장신이,김종묵,김선영,김병문,김원배,김덕경 대한내과학회 2003 대한내과학회지 Vol.64 No.1

저자 등은 기존의 치료에 반응하지 않은 중증 허혈성 지체질환 환자를 대상으로 하여 vascular endothelial growth factor를 이용한 혈관신생 유전자 치료를 시행하였다. 치료 후 환자의 허혈에 의한 하지 통증이 현격하게 감소하고 상처의 진행이 측부혈관이 많이 증가됨이 관찰되어 이에 문헌고찰과 함께 보고하는 바이다. We report VEGF-induced angiogenic gene therapy in a patient with critical limb ischemia, who did not respond to conventional treatment. This patient was the first case in a dose-escalating series of phase I clinical trial. The patient had severe resting pain, gangrene and diffuse ulcer in his left foot. Total 1,000㎍ of naked DNA encoding human VEGF165 was administered intramuscularly to 8 sites of the loft lower extremity. Four weeks after the first 1,000㎛ was administered to the same sites (total dose: 2,000㎛). After gene therapy, resting pain gradually reduced and the amount of analgesics taken by the patient decreased. The ischemic wound of lower extremity slightly improved. However, there was no complete wound healing at 12 weeks of treatment. Digital subtraction angiography at 12 weeks after gene therapy showed an increase in collateral vessels at the mid-tibial, ankle and foot arch levels. Immediately and up to 12 weeks, there was no complication related to gene therapy. These findings may be cautiously interpreted to indicate that intramuscular injection of naked plasmid DNA of VEGF_165 may induce therapeutic angiogencsis in a patient with critical limb ischemia. Further clinical evaluation of VEGF-induced gene therapy is needed to evaluate the safety and efficiency of this treatment.(Korean J Med 64:85-90, 2003)

청각장애자 운동지도를 위한 프로그램 고찰

김정수,박정래,노일환,원충희,김흥식,강신일,박찬홍,김달영,이철원,이찬규 公州大學校스포츠科學硏究所 1987 스포츠科學硏究所論文集 Vol.- No.1

Hearing impairement in varying degrees constitutes one of the most common disabilites affecting school aged children. Eight out of every 1,000 students under the age of twenty have significant hearing loss. Hearing disabilities range from slight to total loss of sound perception. Statistics indicate that only one out of every ten hearing impaired children will become deaf. Most motor activities can be included students may possess low levels of static and/ or dynamic balance, but the greatest majority possess normal motor and physical capabilities. All the hearing impaired youngsters should be properly assessed and given the identical testing batteries as normal children. Hearing impaired students have, essentialy, a communication handicap and suffer from inability to understand verbal instructions. All physical educators should be able to use minimal finger spelling and signing for hearing impaired students.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

연쇄중합효소반응을 이용한 태아성별진단

이희섭,김용신,김화선,김정중,김원신 원광대학교 생명공학연구소 1996 생명공학연구소보 Vol.4 No.1

For sex determination by the PCR method, oligoprimers to Y-chromosome gene, DYZ1, SRY, and AMGL were synthesized and genomic DNA was extracted from male and female placenta for the control use. DYZ1 represented 154 bp single band to 0.001pg/ml male genomic DNA but did not represent 154 bp band in female genomic DNA, SRY represented 341 bp band to 1 pg/ml male genomic DNA but did not represent 341 bp band in female genomic DNA, and AMGL represented 977 and 788 bp double band to 1 pg/ml male genomic DNA and 977 bp single band to 1 pg/ml female genomic DNA in 2% agarose gel electrophoresis stained with ethidium bromide. DYZ1 was 1,000-fold sensitive than SRY and AMGL. DYZ1 and SRY could not identify the PCR failure from female but AMGL identified. To increase the sensitivity, the dual amplification of AMGL was performed and the sensitivity increased to 1,000-fold. During the dual amplification of female genomic DNA mixed with male genomic DNA, 0.00125pg/l, 1:400 part male genomic DNA contamination represented double bands as male. In 2 cases of 46, XY female, DYZ1 and AMGL amplification represented male band but SRY amplification did not represent male band. It was suggested that SRY gene was deleted in two 46, XY female cases. for fetal sex determination, PCR with DYZ1, SRY, and AMGL was performed in 10 cases of chorionic villi and 15 cases of amnionic cells. By the comparison with karyotyping result, fetal sex determination was achieved successfully in all 23 samples using PCR of SRY and AMGL but false result was detected in 3 cases(13%) using DYZ1. According to our results, it was concluded that DYZ1 was 1,000-fold sensitive than SRY and AMGL but could not be used because of its false results, and AMGL and SRY must be used concomitantly for precise sex determination.

권역심뇌혈관질환센터 사업의 비용-편익 분석

김병호 ( Byung Ho Kim ),감신 ( Sin Kam ),배상근 ( Sang Geun Bae ),김건엽 ( Keon-yeop Kim ),이원기 ( Won Kee Lee ),홍남수 ( Nam-soo Hong ),박형근 ( Hyeung-keun Park ),이혜진 ( Heyjean Lee ),조은정 ( Eun-jung Cho ),채성철 ( Shung C 한국보건정보통계학회(구 한국보건통계학회) 2017 보건정보통계학회지 Vol.42 No.1

Objectives: This study was conducted to investigate economic feasibility of operating Regional Cardiocerebrovascular Centers using cost-benefit analysis. Methods: The current study assessed cost and benefit data collected from nine hospitals which were designated as Regional Cardiocerebrovascular Centers between 2008 and 2010. Results: The operation of Regional Cardiocerebrovascular Centers reduced mortality rate and length of stay due to acute myocardial infarction and stroke and also decreased disability occurred after stroke. The total calculated cost of operating nine Regional Cardiocerebrovascular Centers was 19.85-22.30 billion won and the minimum benefit calculated was 23.98 billion won. Applying the maximum cost and the minimum benefit, the net benefit was 1.68 billion won, which is estimated to 190 million won per Regional Cardiocerebrovascular Center. Conclusions: The present study revealed that the operation of Regional Cardiocerebrovascular Centers was economically beneficial. The benefit would be larger if intangible benefit such as the long-term effects to community hospitals and enhancement of quality of life of patients and their families are considered.

조경설계의 대안적 방법에 관한 연구 : 꼴라쥬 기법의 가능성에 대해

김신원,박수영 경희대학교 디자인연구원 2006 예술· 디자인학연구 Vol.9 No.1

Today landscape design looks tendency that is complex and is pluralistic condition. This shows aspect that machine by expression means is more complex being undergone the change by considerations means. We use expression 'Collage' when we speak about complex, hybrid, heterogeneous, pluralism trend, Actually, collage techniques in conversation changed variously flowing cubism, futurism, dadaism, surrealism. Therefore, speech called Collage' that is used in complex expression vocabulary of trend can know that is including phenomenon side as well as techniques side. Approach from collage techniques as art and analyze through literature investigation about background and meaning of techniques, and utilization studied possible techniques in landscape design. With change age through this research, can know whether classical methodology can not express variety of expression method and 'Collage expression' as access methodology of modern landscape design presents possibility of how various expression. Wish to put meaning of research that present methodology freshing complex expression method.

잠재적으로 기능을 갖는 마우스 칼리크레인 유전자(mGK-21) 클로닝과 그 특성에 관한 연구

김화선,이희섭,김원신 원광대학교 생명공학연구소 1996 생명공학연구소보 Vol.4 No.1

Mouse glandular kallikreins are a highly homologous subfamily of serine proteases encoded by 25 genes, all located on chromosome 7^((8)). By protein and cDNA sequence analysis, it was demonstrated that prorenin converting enzyme(PRECE) in the ICR mouse submandibular gland is identical to the epidermal growth factor-binding protein(EGF-BP) type B, the mouse Grandular Kallikrein-13(mGK-13) gene product identified Balb/c^((10, 11)). To examine whether the mGK-21 which cleaves the mouse pro-mouse glandular kallikrein-21(mGK-21) to its mature form is involved in the processing of prorenin, We have cloned the cDNAs of the mGK-21 from a library of male ICR mouse submandibular gland(SMG) and determined the sequences of the cDNA of mGK-21. And then Chinese Hamster Ovary(CHO) cells were transfected with the mGK-21 expression plasmids, and stable cell lines expressing a high level of the mGK-21 precursor were obtained. The conditioned medium from CHO/GK21 cells was then treated with trypsin, since trypsin is known to activate prokallikrein. Therefore, We treated the conditioned medium of CHO/GK21 cells with trypsin, which has been demonstrated to effectively convert the pro mGK-21 to enzymematically active form. Then, the radiolabeled-conditioned medium of CHO/MRB cells, which stablely express mature Ren-2 prorenin, was incubated with the trypsin-treated CHO/GK21. In vitro translation, the product of mGK-21 cDNA was detected and in the northern blotting, mGK-21 were expressed in submandibular gland. In the present study, even though the recombinant mGK-21 do not show a prorenin converting activity, it is apparent that they may possess a serine protease activity.

핵 연료봉 교체 전산화 개발

김영진,신원식,정희철 경희대학교 산학협력기술연구원 2004 산학협력기술연구논문집 Vol.10 No.2

In nuclear power plant, the source of the energy is generated from the nuclear fuel rod. Given a certain level of consumption, the burnt fuel rod should be removed and replaced by a new(fresh) one. The burnt fuel is approximately one third of the whole fuel rods. Currently, this operation is done manually using paper documents and verbal communication and consumes a lot of operation time. In this study, we develop an computerized operation process of nuclear fuel rod replacement procedure based on the ERP(Enterprise Resource planning) methodology.

인천가정지구 공동주택단지 외부공간 기본계획

김신원,윤성호 경희대학교 디자인연구원 2005 예술· 디자인학연구 Vol.8 No.2

New Town is being constructed and planned in and around the Metropolitan area for residential supply, this development will be increased for the residential supply of low income layer according as we forecast landscape architecture plan which in apartment Plans increase. Now constructed apartments is improving in quality but so far a biased function design have bring about insufficiency of community space. This thesis is purposed to present design for revitalization of community space in the landscape architecture plan which in apartment plan.

한국인 여성의 자궁경부암에서 PCR을 이용한 Human papillomavirus 아형의 검출에 관한 연구

김향우,전호종,김윤신,장원재,이미숙,이미자 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The polymerase chain reaction (PCR) was used for detection of human papillomavirus (HPV) in paraffin-embedded tissue. Tissues stored as paraffin blocks were a potential source of DNA for retrospective clinicogenetic analysis. Sections in 6 ㎛ (three or five) thick cut from paraffin blocks of 29 uterine cervix dysplasia, 34 uterine cervix carcinoma in situ, 42 uterine cervix squamous cell carcinoma and 10 uterine cervix metaplasia samples were deparaffinized and incubated at 37℃ for 48hrs. in the lysis solution. Following organic extraction and alcohol precipitation, and extracted DNAs were identified by gel electrophoresis. These DNAs carried out PCR. After repeated cycles of specific oligonucleotideprimed extension of viral DNA with thermophilic DNA polymerase. the type of HPV present was then determined on the basis of the size of the ethidium-bromide stained band visible after agarose gel electrophoresis : for HPV 6 is 263 bp. for HPV 11 is 144 bp, for HPV 16 is 601 bp. and for HPV 18 is 360 bp. This study detected HPV DNA in four cases. which broke down to HPV 16 in two case of uterine cervix carcinoma in situ and two case of uterine cervix squamous cell carcinoma, no HPV type in the remainder. Histologically, HPV types 6, 11 are mainly detected in benign genital warts and lowgrade cervical dysplasias, squamous cell carcinoma frequently contained HPV 16, whereas, HPV 18 was present in adenocarcinoma, adenosquamous cell carcinoma and small cell carcinoma of the cervix. These results indicate that cervical cancers might be clinically influenced by the differences in sutible of the infecting HPV.