Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

Al³^(+) 존재가 ^(99m)Tc-MDP의 표지효율과 생체내 분포에 미치는 영향

장영수,정재민,김영주,곽철은,이동수,정준기,이명철,고창순 大韓核醫學會 1996 핵의학 분자영상 Vol.30 No.3

청소년의 인터넷 중독 정도에 따른 정신과적 공존질환 및 행동 양상에 관한 연구

이준영(June-Young Lee),박이진(E-Jin Park),조근호(Keun-Ho Joe),채숙희(Suk-Hi Chai),김성벽(Sung-Byuk Kim),이창욱(Chang-Uk Lee),김대진(Dai-Jin Kim) 한국중독정신의학회 2010 중독정신의학 Vol.14 No.1

Objectives:This study aimed to clarify the differences in psychiatric comorbidities and, behavioral patterns correlating with the severity of Internet addiction in adolescents. Methods:We assessed 321 adolescents recruited from an Internet rescue school, psychiatric outpatients, and 4 schools in Seoul and Gyeonggido. We divided them into a non-addicted group, a potential-risk group, and a high-risk group according to Young’s Internet Addiction Test (IAT). We evaluated their psychiatric comorbidities and behavioral patterns via psychiatric clinical interview, the Children’s Depression Inventory (CDI), the State-Trait Anxiety Inventory (STAI), and a self-report questionnaire about behavioral pat-terns. Results:There were significant differences in CDI and STAI scores among the 3 groups. The IAT, CDI, and STAI scores correlated positively with each other. There were differences in psychiatric comorbidity distributions between the potential-risk and high-risk groups, but only “other mood disorder” showed a significant difference. There were also significant differences in behavioral patterns among the 3 groups. Conclusion:This study suggests that there are psychiatric comorbidity and behavioral pat-tern differences according to Internet addiction severity in adolescents. We expect further studies will reveal more profound understandings of these relationships.

급성 췌장염의 합병이 추정되는 한국형 출혈열 1예

박영수,김창오,김영근,홍성관,장경희,허애정,염준섭,송영구,김준명 대한감염학회 2001 감염 Vol.33 No.5

The Korean hemorrhagic fever (KHF) is an acute febrile disease with characteristic of fever, bleeding tendency, and renal failure. There are many complications of Korean hemorrhagic fever such as infection, anemia, internal bleeding, hypopituitarism, respiratory, and neurologic complication. A few cases were reported on acute pancreatitis with hemorrhagic fever abroad, but there was no case about Korean hemorrhagic fever with acute pancreatitis in this country. We experienced a case of Korean hemorrhagic fever associated with suspected acute pancreatits. With review of articles, we report a case of 51 year-old woman with KHF, where acute pancreatitis developed during management, (Korean J Infect Dis 33:376∼379, 2001)

전기전도도 측정을 통한 반류수 MLE공정에서 공기공급 제어기술 개발

장신요(Shinyo Chang),신풍식(Pung Shik Shin),정연구(Yeon-Koo Jeong),최영준(Young June Choi) 대한환경공학회 2020 대한환경공학회지 Vol.42 No.3

목적 : 반류수를 대상으로 무산소조에서 전기전도도 측정을 통해 호기조의 공기공급량을 제어할 수 있는 기술을 개발하여 공정성능 향상 및 에너지 사용량 절감을 달성하고자 하였다. 방법 : 전기전도도가 생물학적처리의 운전지표로 활용될 수 있는지를 검증하기 위해 각 공정별 수질인자와 전기전도도의 상관성 분석을 여름철(메탄올 투입), 겨울철, 가을철 시기로 나누어 실험하였다. 기존에 제시된 필요공기량 수식을 간단히 정리하여 경험식을 제시하고, 이를 활용해 전기전도도 범위에 공기공급량을 순차적으로 넣어 조견표를 작성하였다. 성능평가는 암모니아성질소와 총무기질소의 제거효율, SNR과 SDNR, 공기공급량의 변화, 유입수 변화에 대한 공정의 안정성 등을 기준으로 판단하였다. 결과 및 토의 : 계절별로 전기전도도와 각 수질항목의 상관계수가 0.5267 ~ 0.9115 범위로 암모니아성질소, 총무기질소, DOC, 인산염인 순서로 높게 산출되었다. 상관계수 0.5 이상으로 생물학적 처리공정의 운전지표로 전기전도도 가 활용될 수 있음을 알 수 있었다. 전기전도도와 암모니아성질소의 회귀식은 계절별로 재현성이 확보되어 전기전도도를 통해 암모니아성질소를 추정하는 것이 가능할 것으로 판단된다. 호기조 말단 DO가 3.4 mg/L 정도에서 겨울철 질소처리효율이 가장 양호하였다. 호기조 DO는 공기공급량으로 제어할 수 있으며 질소와 상관성이 높은 전기전도도를 직접 측정하여 공기공급량을 제어하고 질소처리효율을 향상시키는 것이 가능함을 알 수 있었다. 전기전도도와 유입유량을 통해 필요공기량을 산정할 수 있는 경험식을 제시하였고, 식 계수를 구한 결과 A", (B" + X") 는 각각 0.0589 (m³-air/h)/(m³/h)/(μS/cm), -77.562 (m³-air/h)/(m³/h)로 나타낼 수 있었다. 겨울철기간 무산소조의 전기전도도 측정값에 따라서 공기공급량을 자동 제어한 결과는 총무기질소 제거효율과 SDNR이 실플랜트 조건보다 각각 8.3%, 0.007 g-N/g-MLSS/d 높게 나타났다. 자동제어한 기간 동안에 공기공급량/유입유량 평균비가 36(m³-air/h)/(m³/h)로 실플랜트 조건보다 공기공급량을 21.7% 감소시킬 수 있었다. 결론 : 전기전도도 기반 MLE공정의 공기공급 제어기술은 질소제거 효율 향상 및 에너지 사용량 절감을 동시에 달성할 수 있을 것으로 판단된다. Objectives : This study aimed to achieve improved process performance and energy saving by developing a technology to control the air supply of an aerobic basin by measuring the conductivity in the anoxic basin. Methods : To verify whether conductivity can be used as an operation indicator of biological treatment, the correlation analysis between water quality factor and conductivity of each process was conducted by dividing into summer (methanol input), winter and autumn periods. An empirical formula was presented by briefly arranging the required air quantity formula, and a quick reference was prepared by putting air supply in the conductivity range sequentially. The performance evaluation was judged based on the removal efficiency of ammonia nitrogen and total inorganic nitrogen, SNR and SDNR, the change of air supply, the stability of the process against inflow change. Results and Discussion : The seasonal correlation coefficients of conductivity and water quality items were calculated in the order of ammonia nitrogen, total inorganic nitrogen, DOC, and phosphate in the range of 0.5267 ~ 0.9115. It was found that the conductivity could be used as an operation indicator of the biological treatment process with a correlation coefficient of 0.5 or more. The regression equations for the conductivity and ammonia nitrogen are secured by season, so it is possible to estimate the ammonia nitrogen through the conductivity. At the end of the aerobic basin DO was 3.4 mg/L, the nitrogen treatment efficiency in winter was the best. The aerobic basin DO can be controlled by the air supply, and it can be seen that it is possible to control the air supply and improve the nitrogen treatment efficiency by directly measuring the conductivity having a high correlation with nitrogen. An empirical formula for estimating the required air volume through conductivity and inflow is presented. A" and (B" + X") are 0.0589 (m³-air/h)/(m³/h)/(μS/cm) and -77.562 (m³-air/h)/(m³/h). The result of automatic control of air supply according to the measured conductivity of anoxic tank during winter season showed that total inorganic nitrogen removal efficiency and SDNR were 8.3% and 0.007 g-N/g-MLSS/d higher than the actual plant conditions, respectively. During the automatic control period, the air supply/inflow average ratio was 36 (m³-air/h)/(m³/h), which could reduce the air supply by 21.7% compared to the actual plant conditions. Conclusions : The air supply can be estimated from the flow rate and conductivity. The air supply control technology of the conductivity-based MLE process will be able to simultaneously improve nitrogen removal efficiency and reduce energy consumption.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

사회인지이론 기반 중산층 노인 식품안전 교육 프로그램의 효과 평가

최정화(Jung-Hwa Choi),유효이(Hyo-E Yoo),정하림(Harim Chung),이혜상(Hye-Sang Lee),이민준(Min-June Lee),장혜자(Hye-Ja Chang),이경은(Kyung-Eun Lee),이나영(Na-Young Yi),곽동경(Tong-Kyung Kwak) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.8

현대의학의 발달은 인간의 수명을 연장시키고 그 결과 노인인구 증가를 가져와 노인 의료비 문제가 사회적으로 대두되고 있다. 이에 적절한 방안으로는 노인들의 건강한 노화(healthy aging)를 도모하는 것이며, 이는 사회의 의료비용을 감소시킴으로써 사회 전체의 효율성을 제고시킬 수 있다. 본 연구는 중산계층 노인을 대상으로 사회인지이론 기반의 식품안전 관리 교육 프로그램을 적용하여 시범실시를 하여 교육 효과 평가를 실시하였다. 본 연구에서는 교육군 212명, 대조군 42명의 자료가 분석되었으며, 사전 사후 지식 총점의 변화는 교육군이 2.80점에서 3.80점으로 유의적으로 상승하였다(P<0.001). 교육군의 식품안전 식태도/건강신념의 교육 전과 후의 총점은 20점 만점에 15.28점에서 16.50점으로 유의적인 상승을 보였다(P<0.001). 대조군은 사전 15.21점에서 사후 15.29점으로 상승하였으나 유의적인 차이를 보이지 않았다. 노인의 식품안전 식행동은 교육군의 교육 전과 후의 총점이 28점 만점에 20.07점에서 22.50점으로 2.43점 상승하여 유의적인 향상을 보였다(P<0.001). 가정 내 식품 취급실태를 살펴보면 식품을 직접 구매한다고 한 응답자가 교육군의 87.3%, 대조군의 95.2%로 나타났으며, 식품 조리 여부 항목에 대해서는 교육군의 87.7%, 대조군의 95.2%가 직접 가정에서 조리한다고 응답하였다. 식태도/건강신념 변화와 식행동 변화값 간에 상관관계가 가장 높으며(r=0.447, P<0.001), 식행동 변화값과 지식의 변화 값과의 상관관계가 높은 것으로 나타났다(r=0.152, P<0.05). 경로별 교육 효과를 보기 위하여 사전, 사후 점수와 점수변화량을 비교 분석한 결과 지식, 식태도/건강신념, 식행동의 모든 경로에서 유의적인 향상을 보였다. 개발된 교육 프로그램의 효과 평가는 2차 시 교육의 사전, 사후 평가를 대조군의 평가 결과와 비교 분석하였는데, 교육의 기간이 2차시로 제한되어 있고 교육 후 행동 변화의 유지를 평가하지 못하였다. 또한, 교육경로와 대상자의 수가 적어 연구 결과를 일반화하기에는 무리가 있으며, 향후 교육의 확대를 통한 후속연구들이 진행되어야 할 것으로 사료된다. This study assessed the educational effectiveness of a developed food safety management education program based on social cognitive theory for elderly in the middle class. The subjects participated in the food safety education program two times at a public health center, community welfare center, senior citizens’ center, and center for children’s foodservice management. For food safety intervention education, data from the intervention group (212 subjects) and control group (42 subjects) were analyzed. Significant (P<0.001) differences in the scores for food safety knowledge, food attitude, and food behavior were observed in the intervention group before and after the study, but no significant difference was observed in the control group. A significant positive correlation also detected between knowledge, food attitude/health belief, and food behavior in the intervention group, which indicates that the study contributed to perception changes in the intervention group and eventually behavioral changes. Accordingly, this program can induce cognitive and behavioral changes by conducting food safety training for the elderly.

장티푸스 진단에 있어서 Vi-Indirect Fluorescent Antibody Test의 민감도 및 특이도

장경희,류동렬,박성하,박병규,이준구,염준섭,최영화,송영구,김현숙,정윤섭,김준명 대한감염학회 1998 감염 Vol.30 No.4

목 적 : 장티푸스는 체액의 세균배양검사에서 Salmonella typhi의 분리로 확진하지만 조기진단을 위해서는 혈청학적인 검사가 도움이 된다. 저자 등은 1989년 이후 장티푸스진단에 S. typhi의 Vi항원을 이용한 간접형광항체법 (Vi-indirect fluorescent antibody test : Vi-IFAT)을 도입하였다. 이에 지난 7년간의 검사결과를 토대로 Vi-IFAT의 민감도 및 특이도를 규명하고자 하였다. 방 법 : 1989년부터 1996년까지 연세대학교 의과대학 세브란스 병원에 발열을 주소로 내원하여 Vi-IFAT가 의뢰된 환자 중 발열의 원인이 확진된 744명의 환자를 대상으로 하였으며, Vi-IFAT에 있어서 양성반응은 1:64이상으로 하였다. 결 과 : 1) 세균배양검사에서 확진된 장티푸스 환자 178예 중 168예에서 양성반응을 보여 민감도는 94.4%이었다. 항체가는 1:64가 89예, 1:128이 36예, 1:256이 27예, 그리고 1:512가 16예이었다. 위음성이었던 10예의 항체가는 1:32가 7예, 1:16이하가 3예이었다. 2) 발열을 주소로 내원한 환자 중 장티푸스 이외의 다른 질환으로 확진된 566예 중 538예에서 음성반응을 보여 특이도는 95.1%이었다. 항체가는 1:32가 117예, 1:16이하가 421예이었다. 위양성이었던 28예의 항체가는 1:64가 23예, 1:128이 5예이었으며, 장티푸스 이외의 살모넬라증이 4예, 요로계감염이 10예, 소화기계감염이 6예, 호흡기 계감염이 5예, 악성 종양이 2예, 그리고 바이러스 감염이 1예이었다. 3) Vi-IFAT 양성인 196예 중에서 장티푸스는 168예로 양성예측율은 85.7%이었으며, Vi-IFAT 음성인 548예 중 장티푸스 이외의 발열질환은 538예로 음성예측율 98.2%이었다. 4) 배양검사와 Widal test 사이의 일치율 (Kappa value)는 0.28로 poor agreement를 보였으며 Vi-IFAT와 Widal test 사이의 Kappa value는 0.74로 good agreement를 보였다. 그러나 배양검사와 Vi-IFAT 사이의 Kappa value는 0.86으로 excellent agreement를 보여 일치율이 가장 높은 검사는 배양검사와 Vi-IFAT라 할 수 있겠다. 5) 장티푸스 환자에 있어서 Vi-IFAT의 양성율은 발열기간이 1중 미만인 환자에서는 68%, 1내지 2주인 환자에서는 89.5%, 2주이상인 환자에서는 100%이었다. 결 론 : Vi 항원을 이용한 간접형광항체법 (Vi-IFAT)은 장티푸스 진단에 높은 민감도 및 특이도를 가지며, 또한 조기진단에 있어서도 임상적으로 유용함을 확인할 수 있었다. Background : Typhoid fever is diagnosed by culture or serological study. The confirmative diagnosis of typhoid fever is made by culture of the causative organism usually from body fluids. Serological test is a supportive diagnositic tool, which is useful for early diagnosis. In Severance Hospital, Vi-indirect foluorescent antibidy test(Vi-IFAT) using the Vi-antigen of Salmonella typhi has been used in the diagnosis of typhoid fever since 1989. We investigated the test results from the past 7 years, in order to clarify the sensitivity and specificity of Vi-IFAT. Methods : A retrospective study was done on patients whose chief complaint was fever and who were tested using Vi-IFAT in the Severance Hospital from 1989 to 1996. The positive value for Vi-IFAT was defined as 1:64 or higher. Results : The sensitivity and specificity of Vi-IFAT for typhoid fever was 94.4% and 95.1%, respectively. The positive and negative predictive values were 85.7% and 98.2% respectively. Positive rates of Vi-IFAT after fever onset increased with time and 68% were positive before the first week. From the first to the second week, 89.5% were positive and after the second week, 100% were positive. Conclusion : Vi-IFAT is not only a valuable serologic test for the diagnosis of typhoid fever, but also useful in the early diagnosis of the disease.

다단계회로의 최소화를 위한 BDD 응용

장준영 대불대학교 1997 論文集 Vol.3 No.1

This Paper presents an application of BDD(Binary DecisionDiagrams) for Minimization of Muli-level Reed-Muller circuits (AND/XOR based circuits). Using BDD, logic functions are represented compactly. The solutionspace and the computing time for synthesizing logic functions are greatly decreased, and this techique can be easily applied to large circuits. The proposed method extracts best patterns to minimize multi-level circuits. Therefore resulting circuits are realized with AND/XOR gates which form a unique class of multi-level Reed Muller circuits with good performance in area optimization and testability. Experimental results using the proposed method show better performance than those using previous methods. Especially, the synthesis method based on BDD shows near-optimal solution for symmetric functions. For large circuits of considering the best input partition and multiple-output, synthesis results have been improved.

방사선치료 조사영역 내에 발생한 설암 환자에서 입체조형방사선치료 경험 : 증례보고

조문준,장지영,김기환,김병국,송창준,김준상,김재성 충남대학교 암연구소 2003 암연구소 업적집 Vol.3 No.-

Objectives : We report an interim result of conformal radiotherapy in a patient with early stage cancer at the base of the tongue, which developed in a previously irradiated area. Materials and Methods : A 64-year-old male patient was diagnosed with T4NOMO supraglottic cancer. He received 72Gy of radiation therapy from 21 November 1988 to 24 February 1989. He had local failure and underwent a salvage total laryngectomy on 28 August 1989. Subsequently, he did well. In early 1999, he suffered from throat pain. He had a 2.5cm ulcerative mass at the base of his tongue, in the area that had been irradiated previously. Biopsy showed squamous cell carcinoma. After workup, he was diagnosed with base of tongue cancer with T2NOMO. Surgery was not feasible because the morbidity was not acceptable. Since it was difficult to re-irradiate the area with a curable dose using conventional 2D radiation therapy with an acceptable morbidity, we decided to try conformal radiotherapy. We used 7 static beam ports with field sizes from 7×6.4 to 8×8㎠, using 6 and 10MV photons. The fractionation regimen was 1.8Gy, 5 times per week. He received 64.8Gy in 36 fractions from 9 April 1999 to 1 June 1999. Results : In the 21 months since radiotherapy, the patient has not experienced any acute or chronic complications, such as xerostomia. He experienced relief of pain shortly after the start of radiotherapy, showed a complete response, and is still doing well. Conclusion : Conformal radiotherapy can be used to treat cancer that develops within a previously irradiated field, with curative intent.