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An immunohistochemical stain for c-erbB-2 oncoprotein and a one step silver stain for AgNORs were performed on the total number of 24 cases of lung cancers. The results were as follows ; 1. c-erbB-2 oncoprotein was detected in 8 of 16 squamous cell carcinomas, 1 of 3 small cell carcinoma, 1 of 2 adenosquamous carcinoma, and 1 of 2 large cell carcinoma. 2. The mean AgNOR numbers percell in lung cancers were higher than those in normal bronchial epithelium. However, there was no difference among histologic types of lung cancers. 3. The mean AgNOR numbers were similar in both c-erbB-2 positive and c-erbB-2 negative squamous cell carcinomas. In summary, the expression of c-erbB-2 oncoprotein is seen in various histologic types of lung cancer and the expression of c-erbB-2 oncoprotein in squamous cell carcinoma may not be related to the cellular proliferative activity by AgNORs.
The colloid silver staining technique to reveal argyrophilic nucleolar organizer regions (AgNOR) was performed in chronic atrophic gastritis, chronic gastritis with intestinal metaplasia, dysplasia, early gastric carcinoma, and advanced gastric carcinoma with or without regional lymph node metastasis. The results were as follows. 1. There was an increasing tendency of the mean number of AgNOR from benign lesions to malignant lesions. 2. The malignant lesions had significantly higher number of AgNOR than benign lesions. 3. The mean number of AgNOR in dysplasia was significantly higher than that of chronic atrophic gastritis. However, there was no statistical difference from other lesions. 4. There was no statistical difference between early gastric carcinoma and advanced gastric carcinoma or between advanced gastric carcinoma without metastasis and advanced gastric carcinoma with metastasis These results suggest that the number of AgNOR may have significance in differentiating between benign lesions and malignant lesions of the stomach, but may not be helpful for evaluating the depth of invasion and metastasis.
To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.
To study angiogenesis and apoptotic indices in the brain tumors, 33 cases of brain tumor(13 cases of astrocytoma, 10 cases of glioblastoma multiforme and 10 cases of meningioma) were seldcted and stained with anti-factor Ⅷ monoclonal antibody and in situ apoptosis detection kit(Apoptag). Among brain tumors glioblastoma multiforme showed the highest angiogenic activity(25.4%). The average percentages of endothelial cells in grade I, Ⅱ, Ⅲ astrocytomas were 3.2, 5.6, 8.4%, respectively and 18.4% in meningioma. Apoptotic indices were highest in glioblastoma multiforme(2.7), followed by grade Ⅲ astrocytoma and meningioma. The results suggest that the glial tumors(astrocytoma and glioblastoma multiforme) seem to show increasing tendency of angiogenesis and apoptotic index ac cording to the degree of malignancy.
The callus was induced from potato tuber on modified MS medium containing 10 mg/1 α-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid 2 mg/1 and somatic embryo was formed in suspension-cultured callus cells. Also, the shoot from microtuber discs was cultured on MS medium And then, we investigated peroxidase activity and patten in callus, somatic embryo induced from potato tuber and shoot induced from potato microtuber in order to study of the physiological and biochemical changes on the developmental stage. The peroxidase activity was higher about 80% in somatic embryogenic cells than callus after 7 weeks culture. And peroxidase isozyme pattern observed 4 cathodic bands and 4 anodic bands by isoelectric focusing and 2 new bands were revealed in somatic embryogenic cells. Addition, in callus 2 cathodic bands and 1 anodic band were revealed, and new pI 3.9 band was observed at 7 weeks culture. The peroxidase activity in shoot induced from microtuber was decreased about 25% cultured shoot for 1 week but increased gradually about 60% after 4 weeks. The pattern of peroxidase isozyme of 4 weeks cultured shoot was observed 2 new bands by isoelectric focusing.
소괴경 형성 단계에 따른 생리학적 연구의 일환으로 peroxidese와 esterase의 효과 활성과 isozyme 양상을 비교 관찰한 결과 소괴경 형성 초기인 배양 7일째 단백질의 함량 및 peroxidase와 esterase의 활성이 가장 높았으며, 발생이 진행됨에 따라 점차 감소되었다. 그러나 단백질 함량과 esterase 활성은 성숙단계인 배양 60일째에서 증가하였다. 이는 괴경형성 초기에 새로운 단백질들이 합성되어 축적되고 이러한 과정에서 peroxidase와 esterase의 활성이 증가되는 것으로 사료된다. 전체 단백질 전기영동 양상은 서로 다르게 나타났으며, 소괴경에서 40 KD의 patatin이 main band로 나타났다. Peroxidase isozyme 양상을 비교 관찰한 결과 모든 단계에서 10개의 band가 나타났으며, pI 6.72의 band가 주 band로 나타났다. Esterase isozyme 양상은 pI 4.48의 band가 주 band로 나타났으며, 배양 15일에서부터 pI 5.78의 band가 나타났다. 그리고 배양 60일째에서는 pI 7.52의 새로운 band가 나타났다. 그러므로 이들 isozyme 양상의 변화는 감자의 분화와 발생에 중요한 작용을 하는 것으로 사료된다. In order to investigate physiological change depending on the developmental stage of microtuber, the microtubers formed in SH medium were examined the changes of peroxidase and esterase activity, and their isozyme patterns as well. Protein content, peroxidase and esterase activities were the highest at the 7 day-culture of the microtuber and then gradually decreased on the microtuberization, whereas protein content and esterase activity increased at the mature stage of the 60 day-culture. Therefore, these results indicate that a specific group of proteins was deposited at the early stage of tuberization and increased peroxidase and esterase activity. In addition, total protein patterns were different on the SDS-PAGE, and were appeared the patatin as main bands. Peroxidase isozyme patterns were appeared 10 bands in all stages, and were appeared bands of pI 6.72 as main. Also, Esterase isozyme were exhibited different pattern, and main bands of pI 4.48, new bands of pI 5.78, and pI 7.52 were appeared from the 7 day-, 15 day-, and 60 day-cultured microtuber, respectively. These results suggest that the changes of peroxidase and esterase activities and isozyme patterns are important factor in the differentiation and development of potato.