관상동맥질환 환자에서 스텐트 시술 후 재협착에 관한 연구

강진환,권영주,박상호,한대희,이상철,김명구,변정득,최병조,심규혁,온영근,현민수,김성구 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background and aims : In the coronary artery disease, a rate of restenosis was much decreased by placement of coronary stent than when percutaneous transluminal coronary angioplasty has been applied, but there are still important clinical problems of restenosis. There has been many progressive studies about various factors contributed to this restenosis. So, we studied a relation between restenosis after placement of coronary stent in the coronary artery disease and many clinical factors, characteristics of stenotic lesion and procedural factors, and also assessed the restenosis rate of various coronary stents. Methods : Total 58 lesions(46 cases of patients ; man 30, woman 16) were evaluated, which has been performed a follow-up coronary angiography after 6 months (mean 188 days) since coronary stent had been placed. Various stents were implanted and assessed a relation between restenosis and many factors. Results : Of 58 lesions were target stenotic studies, there were 22 of restenotic lesions(37.9%), and the restenosis rate wes statistically significant difference in the relation with diabetes mellitus(p<0.05), group of acute myocardial infarction(p<0.05) among clinical diagnosis of ischemic heart disease(stable angina, unstable angina and acute myocardial infarction) in clinical factors and with high dilation pressure of stent(p<0.05) in procedural factors. When the rate of restenosis was evaluated among implanted stents, it was lowest in the Multilink^(®) stent and highest in the Nir Royal^(®) stent(60%). Conclusion : In this study of restenosis and various factors after placement of stents in the coronary artety disease, factors such as diabetes mellitus, group of acute myocardial infarction and a dilation pressure of stent were significant related with restenosis and the restenosis rate of Multilink^(®) stent was lowest and the highest restenosis rate was of Nir Royal^(®) stent.

서울시 중년층이 선호하는 미래 노인 주거환경Ⅱ : 관련변인이 노인주거환경 요구에 미치는 영향 The Effects of the Related Variables on the Demands for the Residential Environments for the Elderly

강순주,전명숙 건국대학교 생활문화연구소 1995 생활문화ㆍ예술논집 Vol.18 No.-

The purpose of this study was to examine the environmental characteristics of housing for thr eld- erly preferred by the middle aged. The residential environments include three types of elderly hous- ing differred in the availability of care service as well as inside and outside environmental characteristics of the housing. The independent variables were socioeconomic characteristics of the respondents, the levels of participating in various social activities, and attitudes toward aging. Six hundreds and fifteens males and females aged 40 to under 60 in Seoul were selected for the empherical study. The statistical procedures in SPSS PC+ were used to analyze the data. The results of this study were as follow. In general, the inside residential environments were more highly demanded by the future elderly than were the outside environments. The demands for the in- side and outside environmental characteristics were not the same among independent variables such as education, income, health, levels of participating in social activities, and attitudes toward aging. The demands for the inside and outside environmental characteristics were varied according to the socioeconomic characteristics of the future elderly, the levels of participating in social activities, and the preferred type of elderly housing. The following study should be extended to the elderly in rural areas and also be included various characteristics of the residential environments. The policymaker.s of the elderly housing should con- sidcr the demands for the care service as well as for the environmental characteristics are varied in individuals of the future elderly.

돌와(Gynostemma pentaphyllum)엑기스가 白鼠의 肝臟障碍에 미치는 影響

丁明鉉,文永熙,姜壽鐵,李炳柱 조선대학교 약학연구소 1989 藥學硏究誌 Vol.11 No.1

This study was attempted to invesigate the effects of extract and crude saponins of Gynostemma pentaphyllum Makino on the activities of S-GPT, S-GOT, Al.P, LDH and the levels of total-bilirubin, total-cholesterol in the serum of nomal rats and experimentally CCl_4-intoxicated rats, and on the effect of body, liver and spleen weight in CCl_4-intoxicated rats. The results were shown as follows; The extract (GE.) and crude saponins were showed a significantly decrease in the activities of S-GPT, S-GOT, Al.P and the level of total bilirubin in the serum elevated by CCl_4, but crude saponins was more decreased than GE. The activities of LDH were significantly decreased of the 4th and the 6th days after administration of GE., and crude saponins was decreased in proportion to the number of days. The level of total cholesterol were significantly decreased in all sample groups. when compared with control group, but remarkably decreased in GE. 500㎎/㎏ and crude saponins 100㎎/㎏. The liver and spleen weight were decreased on the administration of GE. and crude saponins. According to the above results, it is thought that GE. and crude saponins exhibited significant antihepatotoxic activity in the CCl_4-intoxicated rats.

만성 심부전환자의 혈액에서 측정한 Tumor necrosis factor-α 및 Interleukin-6의 임상적 의의

김명구,김성구,박상호,한대희,강진환,변정득,심규혁,최병조,방덕원,온영근,현민수,권영주 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background and objectives: Many conditions are responsible for the pathophysiology and progressive mechanisms of congestive heart failure. More recently, it has also become evidence that another class of biologycally activated molecules generically reffered to as cytokine these are also over expressed in congestive heart failure. Therefore, the purpose of this study was to measure concentrations of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) in mild to severe symptoms of heart failure and compare their values with those found in normal control and analysed correlation relationship between cytokine level, clinical findings and hemodynamic indicies. Subjects and Methodology: Levels of TNF-α and IL-6 were measured on pulmonary artery during cardiac catheterization in heart failure patients(n=32) and normal subjects(n=8) as well as physical examination and echocardiogram. Cytokines assay were performed on plasma using commercially available ELISA(Enazyme-Linked Immunosorbent Assay) kits. Results: Although the levels of TNF-α and IL-6 tend to increase in congestive heart failure group, the cytokines level was not made significantly statistical difference between congestive heart failure group and controls. When analyzing the correlation between the levels of PCWP(pulmonary capillary wedge pressure) and cytokines(TNF-α, IL-6), respectively, there were statistically significant correlation coefficient 0.32,(p<0.05), 0.39(p<0.01). The cytokine IL-6 and pressure of pulmonary artery were significant correlation.(correlation coefficient 0.36, p<0.02) More significantly, there was correlated with TNF-α and IL-6.(correlation coefficient 0.57, p<0.001) Conclusions: There was tended toward high concentration of TNF-α & IL-6 in congestive heart failure and significant difference for PCWP between TNF-α & IL-6, thus may be correlated with development and progression in congestive heart failure.

폴리머타임을 이용한 슈퍼그래픽 개발

이명호,이주희,이강우 강원대학교 석재복합신소재제품연구센터 1998 석재연 논문집 Vol.3 No.-

활성화된 마그네슘을 이용한 Michael 반응에 관한 연구

맹주양,김명희,강현구 순천향대학교 기초과학연구소 2000 순천향자연과학연구 논문집 Vol.6 No.1

The Most powerful tools in the synthetic organic chemists arsenal are organometallic compounds and metal mediated transformations. We reported a general approach for highly reactive metal powder by reducing metal salt in tetrahydrofuran solvents using lithium as reducing agents. Organometallic compound was generated by reaction of metal powder with organo compounds and then it was reacted with α ,β -unsaturated ketone. We synthesized 3-(4-chlorophenyl ) -1- (2-thienyl)ethylpropenoate, ethyl- (2-cyano) -3- (thienyl ) -propenoate and ethyl-(2-cyano)-3-(4-chlorophienyl)-propenoate, which are α , β -unsaturated ketone derivations. Magnesium chloride react with Li+Naph-r to give activated magnesium. The synthesized activated magnesium metal reacted with sec-Butylmagnesium chloride and n-Butylmagnesium bromide to give alkyl magnesiumhalide. The active amyl magnesium derivation reacted with the synthesized α , β-unsatruated ketone to give product.

감성돔(Acanthopagrus schlegeli) 유래의 주화세포의 확립과 확립된 세포의 특성

임은영,강민수,오명주,정태성,정성주 한국어병학회 2003 한국어병학회지 Vol.16 No.3

감성돔 (Acanthopagrus schlegeli)의 비장에서 주화세포인 BSBS를 확립하고 세포의 특성을 검사하였다. BSBS세포는 60회 이상의 계대배양이 가능하였고 형태학적으로는 상피성 세포였다. 세포는 20℃, 10%의 FBS가 든 L-15배지에서 배양하는 조건에서 잘 자랐다. BSBS세포는 해양버나바이러스 (MABY Y-6), 잉어의 봄바이러스병바이러스 (SVCV), 넙치의 랍도바이스(HIRRV)와 연어바이러스 (CSV)를 접종했을 때 세포변성효과가 나타났다. 새로 확립된 주화세포는 앞으로 많은 바이러스병의 연구에 유용하게 사용할 수 있을 것으로 생각된다. A stable cell line, BSBS (black seabream spleen), was established from the cells in spleen of black seabream, Acanthopagrus schlegeli, and characterized. Subculture maintained more than 60 passages and mophologically, BSBS cell was epithelioid cell. The cells grew optimally at 20℃ in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum with incubation temperature of 20℃. BSBS cells supported the growth of marine birnavirus (MABV Y-6), chum salmon reovirus (CSV), spring viremia of carp virus (SVCV) and hirame rhabdovirus (HIRRV). Thus, the new cell line may be useful for studying wide range of fish viruses.

흰쥐 뇌세서의 Cholecystokinin mRNA 함유신경세포의 분포

김우태,배명애,이호섭,박매자,배용철,홍해숙,조희중,주강 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.4

목적 : 뇌에서 Cholecystokinin(CCK)-8 함유신경세포의 분포에 대해서는 면역조직화학적기법을 이용하여 행한 연구가 많으나 CCK mRNA함유신경세포의 분포에 대한 연구는 아직 단편적이므로 본 연구에서는 이들 신경세포의 분포를 정확히 밝히고져 하였다. 대상 및 방법 : 흰쥐 뇌에서 ^35S가 표지된 oligonucleotide를 이용한 in situ hybridization기법으로 관찰하였다. 결과 및 결론 : 종뇌; 후구의 전후핵, 신피질, 이상엽, 후내야, 외측, 기저 및 피질 편도핵, 해마체, 그리고 선조체에서 CCK mRNA함유신경세포가 관찰되었다. 간뇌; CCK mRNA함유신경세포가 시상의 경우 전내측시상핵, 전복측시상핵, 외측시상핵, 내측시상핵, nucleus reunions, nucleus rhomboideus, 그리고 내측슬상핵에서 관찰되었으며 시상하부의 경우는 시삭상핵, 실방핵, 등내측시상하부핵, 그리고 유두상핵에서 관찰되었다. 중뇌; 흑질의 밀집부, 복측피개야, nucleus linea rostralis, 중심회색질, Edinger-Westphal nucleus, 그리고 하구에서 CCK mRNA 함유신경세포가 관찰되었다. 교 및 연수; CCK mRNA 함유신경세포들이 lateral parabrachial nucleus 삼차신경척수로고유핵, 거대그물구성체핵, 연수그물구성체, 그리고 삼차신경척수로핵에서 관찰되었다. 이상의 결과를 지금까 보고된 CCK-8함유신경세포의 분포에 대한 면역조직화학적 연구결과와 비교 하였을 때 대부분의 부위에서 CCK-8 및 CCK mRNA 함유신경세포의 분포가 유사하였으나 시상의 경우 CCK-8 함유신경세포는 검출되지 않은 반면 매우 많은 수의 CCK mRNA 함유신경세포가 존재함을 알 수 있었고 대뇌 피질의 경우 CCK mRNA 함유신경세포수가 CCK-8 함유신경세포보다 월등히 더 많았다. 반면, 등측봉선핵 및 고속핵에서는 CCK-8 함유신경세포가 다수 존재함이 보고되어 있으나 본 연구의 경우 CCK mRNA 함유신경세포를 관찰할 수 없었다. In order to investigate the distribution of cholecystokinin(CCK) mRNA containing neurons in the rat brain, brains were extirpated rapidly, frozen sectioned and processed for in situ hybridization technique with ^35S labeled oligonucleotide probe. The results obtained were as follows Telencephalon; CCK mRNA containing neurons were found in the anterior olfactory nucleus, neo-cortex, piriform cortex, entsorhinal cortex, lateral, basal and cortical amygdaloid nucleus, hippocampus and corpus striatum. Diencephalon; In the anterior medial thalamic nucleus, anterior ventral thalamic nucleus, lateral thalamic nucleus, medial thalamic nucleus, nucleus reuniens, nucleus rhomboideus, and medial geniculate nucleus, CCK mRNA containing neurons were found. In the hypothalamus, CCK mRNA containing neurons were located in the supraoptic nucleus, paraventricular nucleus, dorsomedial nucleus and supramammillary nucleus. Midbrain; CCK mRNA containing neurons were found in the substantia nigra pars compacta, ventral tegmental area, nucleus linea rostralis, substantia gricea centralis, Edinger-Westphal nucleus and inferior colliculus. Pons and Medulla oblongata; CCK mRNA containing neurons were found in the lateral parabrachial nucleus, nueteus originalis nervi trigemini, nucleus reticularis gigantocellularis, nucleus reticularis medullae oblongatae, nucleus tractus spinalis nervi trigemini.

Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.