榮山湖 上流支術의 水質에 관한 硏究 : 砥石江을 中心으로 on the Ji-Suk river

朱興珪,吳東植 조선대학교 약학연구소 1991 藥學硏究誌 Vol.13 No.1

Physico-chemical and microbiological research of water quality of Ji-Suk river, one of the 3 tributary streams of the upper stream Lake Youngsan, was performed twice in August 1990, and September 1990, and results are obtained this research as the following: The pH of the whole research area is 6.3 to 7.5, no much difference from those of common streams in Korea, which means that there is no special factors to change the pH around Ji-Suk river. The DO is 5.8ppm to 8.5ppm and shows a little difference from area to area of observation. It is considered that the water is contaminated by waste water from the 2 large amusement parks from Site 1 to Site 5. The BOD is 3.1ppm to 3.5ppm except Site 1, no much difference from area to area of observation, which makes comparayively stable quality of river water. It is because Site 1 is where the 3 tributary streams of the upper stream from Lake Youngsan joins together, and it contains a lot of organic substances to increase BOD higher than other sites of observation. Nut-ritive salts are observed to be comparatively low : 0.02ppm to 0.08ppm of NH_3-N, 0.03ppm to 0.08 ppm of NO_2-N, 2.23 ppm to 4.00ppm of NO_3-N, and 0.022ppm to 0.081ppm of PO_4-P, over all research area, But in Site 1, 0.08ppm of NH_3-N, 0.08 ppm of NO_3-N, and 3.6 ppm of NO_3-N are observed. It says that various nitrogen compounds flow into the upper stream of the river to to remain as they were or to be nitrified at this site, the most upper stream of Ji-Suk river. Each of Cl-ion and hardness : 37.02 ppm to 39.90 ppm, 37.02 ppm to 45.10 ppm, respectively : is analyzed rather homogenously all over the research area. Compared to other rivers, such substances are found from the water of this river not due to the point source contamination, but mainly due to the quality of soil. Normal bacteria and E. Coli are observed to be comparatively lower in this research area than other river, still from 6.8×10^2 to 1.8×10^4 of E. Coli is analyzed at each research site. It proves, however, that this river is contaminated generally by fecal excrete from the 3 large amusement parks along Ji-Suk riverside, and some special measures are demanded to manage and protect these amusement parks.

Folate 拮抗劑의 合成硏究

高玉鉉,姜馨龍,李敦日,金永洙,白采善,金京洙 조선대학교 약학연구소 1990 藥學硏究誌 Vol.12 No.1

In order to synthesize Methotraxate(MTX) analogues, author synthesized 3-〔〔〔2-(N-Ethyl-N-tosyl-p-aminobenzenecarboxamido)ethyl〕thio〕methyl〕-2-methylimidazo-〔1,2-a〕pyridine(15), 2-(N-Ethyl-N-toyl-p-aminobenzoyl)-5-phenyl-2H-tetrazole(16),2-(N-Ethyl-N-tosyl-p-aminobenzoyl)-5-(nitrobenzyl)-2H-tetrazole(17), 3-〔〔〔2-(N-Ethyl-p-aminobenzenecarboxamido〕ethyl〕thio〕methyl〕-2-methylimidazo〔1,2-a〕pyridine(18), 2-(N-E-thyl-p-aminobenzoyl)-5-phenyl-2H-tetrazole(19), 2-(N-Ethyl-p-aminobenzoyl)-5-(4-nitro-benzyl)-2H-tetrazole(20), 2-Amino-3-cyano-5-〔3-〔〔〔2-(N-ethyl-p-aminobenzenecarboxa-mido)ethyl〕thio〕methyl〕-2-methylimidazo〔1,2-a〕pyridino〕methyl pyrazine(21), 2-Amino-3-cyano-5-〔2-N-ethyl-p-aminobenzol)-5-phenyl-2H-tetrazol-2-yl〕methylpyrazine(22), 3-A-mino-3-cyano-5-〔2-N-ethyl-p-aminobenzol)-5-(4-nitrobenzyl)-2H-tetrazol-2-yl〕methylp-yrazine(23), 2,4-Diamino-6-〔3-〔〔〔2-(N-ethyl-p-aminobenzenecarboxamido)ethyl〕thio〕methyl〕-2-methylimidazo〔1,2-a〕pyridino〕 methylpteridine(24), 2,4-Diamino-6-〔2-(N-ethyl-p-aminobenzoyl)-5-phenyl-2H-tetrazol-2-yl〕methylpteridine(25), and 2,4-Diamino-6-〔2-(N-ethyl-p-aminobenzol)-5-(4-nitrobenzyl)-2H-tetrazol-2-yl〕methylpteridine(26), respectively.

玉蜀黍毛 水性엑기스의 賢臟作用

羅漢光,李漢九,高錫太 조선대학교 약학연구소 1991 藥學硏究誌 Vol.13 No.1

Stigma Maydis Water Extract (SWE), when given intravenously, produced the diuretic action accompanied with the increased amounts of sodium (E_Na) and potassium (E_K) excreted in urine, and the decreased reabsorption rates of sodium (R_Na) and potassium (R_K) in renal tubules, and then glomerular filtration rate (GFR) and renal plasma flow (RPF) were not changed, osmolar clearance (Cosm) was augmented, but free water clearance (??) was diminished. SWE, when given into a renal artery, or into carotid artery, elicited the decreased urine flow, GFR, RPF, E_Na the unchanged R_Na, E_K and R_K in both kidney. Above results suggest that SWE exhibit the central antidiuretrc action, and diuretic action by inhibition of electrolytes in renal tubules through humoral natriurectic agent.

Ascorbic Acid가 Ferrous Sulfate의 Rat 소장흡수에 미친 영향

이진환,최준식,범진필,유철진 조선대학교 약학연구소 1991 藥學硏究誌 Vol.13 No.1

This paper was attempted to investigate the effect of ascorbic acid on the dissolution rates, the partition coefficient and the absorption rates of ferrous sulfate from rat small intestine. Ascorbic acid increased the dissolution of ferrous sulfate using basket method. Ascorbic acid increased the partition coefficient of ferrous sulfate in n-octanol and phosphate buffer (pH4.0)system. The dissolution rate and the partition coefficient of ferrous sulfate were increased by increasing dose of ascorbie acid. Ascorbic acid significantly enhanced the absorption rate of ferrous sulfate from rat small intestine. The absorption rate was enhanced by increasing dose of ascorbic acid. The correlation of the dissolution rate and the absorption rate, and the correlation of partition and the absorption rate were found the linear relationship respectively in presence of ascorbic acid. From the results of this experiment, it can be concluded that ferrous sulfate combined with ascorbic acid is more available than ferrous sulfate single for both anemia therapeutics and redusing side effect.

Methyl Orange에 의한 Benzalkonium Chloride의 Ion Pair 抽出 吸光度定量

金永洙,白采善,崔宗煥 조선대학교 약학연구소 1989 藥學硏究誌 Vol.11 No.1

Phthalein and azo dyes have been used by forming ion pair agents. Primary, secondary and tertiary amines in aliphatic amines have been fully examined, but aliphatic quaternary amines or their derivatives have not been researched satisfactorily until now. In this thesis, the author investigated the application of extraction method of organic solvents in forming ion pair between quaternary ammonium salts, and methyl orange (MO). In the process of laboratory work, MO and benzalkonium choride(BKC) were mixed and added the buffer solution to 10㎖, and then the solution was shaken for 5minutes. The maximum absorption wavelength of the above stable solution which was extracted with dichloromethane was 420nm. The maximum absorption wavelength of MO aqueous solution was 464nm, and it shifted 44nm toward the long wavelength from 464nm. In this experiment, the most suitable pH range was 2∼8. Dichloromethane was the best of all other solvents as than 1.2-dichloroethane, chloroform, benzene, nitrobenzene and carbon tetrachloride in the extraction solvents of ion pair which was formed between MO and BKC. However, MO has never been extracted with any kinds of solvents either. By using the method of continuous variation, the author investigated that the mole ratio of reaction products formed between MO and BKC was 1:1 ratio. When made the BKC calibration curve to MO in best experiment condition, the Lambert-Beer's law was obeyed in the range of BKC. concentration 3×10^-5∼1.0×10^-4 M.

榮山湖 上流支術의 水質에 관한 硏究 : 極藥江을 中心으로 On the Geuk-Rak river

朱興珪,文大中 조선대학교 약학연구소 1992 藥學硏究誌 Vol.14 No.1

Physico-chemical and microbiological research of water quality of Geukrak River, on of the three tributary streams of the upper stream from Lake Youngsan, was performed in February 1992, on March 1992, and results are obtained from this research as the following: 1. The pH of the whole research area is 7.0 to 7.6, comparatively homogeneous of all area, which says that there is no special factors to change the pH around Geukrak River noticeably. 2. The DO is 5.7ppm to 9.0ppm at the first observation, and 5.4ppm to 8.8ppm a little bit lower at the second observation, but it is distributed in similar pattern througout all research area. 3. The BOD is 2.9ppm to 3.7ppm except Site l and Site 3 during the whole research period, that makes comparatively stable quality of lake water. But it is 4.1ppm and 4.8ppm at Site l, and 4.7ppm at Site 3, owing to the influence of various untreated organic substances from the outlets of the urban life sewage treatment plant of Kwangju City. 4. Except Site 2 and Site 3, 1.0ppm to 5.8ppm of NH_3-N, 0.08ppm to 0.29ppm of NO_2-N, 1.15ppm to 3.20ppm of NO_3-N, and 0.01ppm to 0.04ppm of PO_4-P are observed, all comparatively low. But NH_3-N and PO_3-P are observed to be 5.5ppm to 8.0ppm, 8.3ppm to 10.5ppm, and 0.06ppm to 0.08ppm at Site 2 and Site 3, higher than other areas due to the same cause as in case of BOD. 5. Each of Cl-ion and Hareness are 27.02ppm to 36.87ppm and 22.40ppm to 37.52ppm, respectively, at the first observation, and 33.01ppm to 41.48ppm, and 44.14ppm to 76.01ppm, respectively, at the second observation. A little bit of increase is found at the second observation from the first observation, but it is considered that this increase is not due to dangerous point source contamination, compared to other rivers, but maily due to the quality of soil. 6. Normal bacteria and E. Coli are 1.8×10^2 to 2.4×10^4, and 1.2×10^2 to 1.9×10^4 over the whole research area. It shows diverse distribution, but pretty low compared to other rivers. However, 2.2×10^3 to 1.9×10^4 of E. Coli analyzed from Site l to Site 3. and this fact draws special attention to the management of water quality of Geukrak River, considering that these sites are the closest to the main stream of Lake Youngsan.

고속액체 크로마토 그라피(HPLC)에 의한 Human Plasma중 Pyenytoin의 분석

金義泰,白采善 조선대학교 약학연구소 1993 藥學硏究誌 Vol.15 No.1

A procedure for analysis of phenytoin in human plasma by High Performance Liquid Chromatograph was developed and evaluated. Phenytoin was analyzed isocratically on a reverse phase column with a mobile phase consisting of 40% methanol+10% acetonitrile+0.1% phosphoric acid, PH4.2. The eluated phenytoin was detected at 210nm, and quantities estimated from peak height ratios. Phenytoin concentration was quantitated to range from 3㎍/ml, total recovery for a sample was 93.3%%-94.1%.

뇨중 폐노프로펜의 HPLC분석

백채선,김의태 조선대학교 약학연구소 1998 藥學硏究誌 Vol.19 No.1

A rapid high-performance liquid chromatographic(HPLC) method for quantitative determination of fenoprofen in urine is described. The assay was performed after single extraction of fenoprofen and ibuprofen (internal standard) from urine using acetonitrile and eluted from a 5 pm C18 reversed phase column. The mobile phase consisted of acetonitrile + 0.5% phosphoric acid(50 : 50 v/v%) pumped isocratically at a flow rate of 1.0 mL/min. The effluent was monitored at 220 nm. Quantification was achieved by the measurement of the peak hight ratio, and the absolute recoveries ranged from 94.3 t o 103.0. Intra-day coefficients of variation(CV) ranged from 1.95 to 4.59%. and Inter-day coefficients of varidations(CV) ranged from 2.17 to 5.20 %

개에 있어서 Chloramphenicol의 尿 및 擔汁中 排泄機轉에 관한 硏究

金成源 조선대학교 약학연구소 1978 藥學硏究誌 Vol.1 No.1

1) A study on the mechanism of biliary and urinary excretion of chloramphenicol has been performed in the dog. 2) Chloramphenicol administered intravenously to dogs with ligated renal pedicle, readily appeared in bile in concentration greater than in plasma. 6.0% of a 50㎎/㎏ Ⅰ.Ⅴ. dose of chloramphenicol were excreted into bile within 100minutes, During the same periods of above experiment, the bile/plasma concentration ratios(B/P ratios) were 46 to 87. 3) Chloramphenicol injected into the vein of dog was rapidly excreted into urine. 18% of the administered does were excreted into urine within 70 minutes. In the same periods of this experiment, Ccm/Ccr ratios were greater than 1.0 in most cases. 4) In experiment of simultaneous measurement of biliary and urinary excretion of chloramphenicol, Ccm/Ccr ratios were less than 1.0 and B/P ratios were 50 to 52. 5) In experiment measured simultaneously biliary and urinary excretion both Ccm/Ccr and C^H_cm (hepatic clearance) were significantly declined by probenecid, but not affected by 2,4-DNP and aminophylline although 2,4-DNP increased only bile flow and aminophylline both bile and urine volume. 6) Ccm/Ccr and C^H_cm were increased in proportion to increment of plasma concentration ranging from 3.3 to 30㎎%, of chloramphenicol. But when plasma concentration were increased to 70㎎%, Ccm/Ccr were not increase and C^H_cm were reduced about 30% in comparison with values obtained at 30㎎% of chloramphenicol. 7) Free/Bound(free to boumd form) ratios ranging from 1.0 to 90㎎% of chloramphenicol were 76.2+3.72%(mean+S.E.) Above results suggest that chloramphenicol is exereted into bile by a process of active transport, that excretion of chloramphenicol into urine was made up with dual process, reabsorption and secretion, and that renal secretion was attained by active transport process although renal reabsorption process could not understand.

신장장해 가토에서 Theophylline의 약물동태

차중권,이진환,최준식,김가나,범진필 조선대학교 약학연구소 1992 藥學硏究誌 Vol.14 No.1

This study was attempted to investigate the pharmacokinetics of theophylline in rabbits with folate induced renal failure. Plasma concentration and AUC of theopylline were increased in rabbits with folate induced renal failure compared with those of normal rabbits. Renal clearance and total body clearance were decreased in rabbits with folate induced renal failure compared with those of normal rabbits. Correlation of serum creatinine concentration and AUC, and correlation of serum creatinine clearance and renal clearance have linear relationship respectively. From the results of this experiment, it is desirable that dosage regimen of theophylline should be adjusted for effective and safe therapy in patient with renal failure in clinical pharmacy practice.