한국어병학회 회칙 ( 한국어병학회지 창간호 , 1988 . 5 )

학회자료 한국어병학회 1988 한국어병학회지 Vol.1 No.1

2012년 고수온기에 발생한 남동해권역 조피볼락의 대량폐사

이덕찬 ( Deok Chan Lee ),박영철 ( Yong Chul Park ),전창영 ( Chang Yung Jeon ),양준용 ( Joon Yong Yang ),허영백 ( Young Baek Hur ),김진우 ( Jin Woo Kim ),조기채 ( Ki Chae Cho ) 한국어병학회 2013 한국어병학회지 Vol.26 No.3

From July to early September 2012, there was mass mortality of fishes, particularly black rockfish, which were being raised in the floating fish cage along the coast of Gyeongsangnam-do. The amount of damage was 1,802,000 fishes and the causes were confirmed to be rapidly rising water temperature and repeated daily changes in water temperature. The water temperature in this area of the sea rose to the maximum 28.4℃ and the daily range of changes in water temperature was maximum 6.5℃. As a result of investigating biological diseases of 194 fishes in 49 fishery areas, major pathogenic organisms such as red seabream iridovirus (RSIV), Vibrio sp. and Vibrio spp. or Microcotyle sp. were detected in rockfish in some fish farms. It is considered that the major causes of the mass mortality were high water temperature accompanied by repeated daily changes in water temperature, it is considered that biological diseases influenced the increase in the perish of fishes.

냉수성 질병에 대한 제주 양식 돌돔, Oplegnathus fasciatus의 병리조직학적 관찰

문경미 ( Kyung Mi Moon ),고대희 ( Dae Hee Ko ),김동휘 ( Dong Hwi Kim ),박근태 ( Geun Tae Park ),허문수 ( Moon Soo Heo ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2

The outbreak of haemorrhagic speticemia and dermal lesions in cultured Rock sea breem in Jeju Island are frequently associated with keratitis. Prolonged infection leads to the symptoms accompanied by necrosis of fin and skin and the fishes died. Present study aims to study the histopathological changes of diseased fish muscle tissue. The tissue at the initial stage of infection showed normal muscular texture, however in the late stages of infection, muscular lesion had been expanded and hence, necrosis had progressed deeply into muscle tissue. Mild tissue in caudal fin was found with more necrosis, and gill tissues were also collapsed. In Kidney, renal tubules were teared along with tissue destruction (Espada, J et al, 1993). Hence in the present study, we examined the histopathological variation of the infection fishes, and this basic data would be used for future research of in haemorrhagic specticemia and dermal diseases.

월동장의 참동에서 분이한 비브리오균에 대하여

박수일,최혜승,윤장근 한국어병학회 1988 한국어병학회지 Vol.1 No.1

越冬實驗中인 참돔 病魚에서 病原菌을 分離하여 그 性狀을 比較檢討하였다. 그 結果, 原因菌은 生物學的, 生化學的諸性狀으로부터 Vibrio sp.로 同定되었다. 日本에서 報告된 참돔 由來의 Vibrio sp. (第III群)와는 重要性狀에서 差異가 났다. 分離由來가 다른 Vibrio sp. (第II群)와는 여러 性狀에서 一致하므로 低溫에서의 發育能이 差異가 나는 生物型으로 생각되었다. Three strains of pathogenic bacteria isolated from diseased red sea bream at a culture farm in Gyoung sangnam-Do during the midwinter of 1987 were studied taxonomically. Based on their biological and biochemical characteristics, they were identified as Vibrio sp., They exhibited very similarities to group II (Kusuda et al.,1979) except that the former can grow at lower temperature.

시판 전복박리제의 박리 효과

김위식 ( Wi Sik Kim ),강민호 ( Min Ho Kang ),김종오 ( Jong Oh Kim ),이시우 ( Si Woo Lee ),김정 ( Jung Kim ),황두진 ( Doo Jin Hwang ),오명주 ( Myung Joo Oh ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2

Three commercial exfoliating reagents, product A (main components: citric acid and vitamin C), B (herb) and C (nicotinamide), were used to study their exfoliation effect on abalone, Haliotis discus hannai from the substrate. The exfoliating reagents A, B, C and oxytetracycline (OTC, control) of 6-31.25 g/L and 12-37.5 g/L exfoliated 81.2-84.8% and 90.3-95% of abalone, respectively. Post-treatment recovery time for the abalone was similar for all the reagents except product C. Recovery period for the abalone immersed in OTC for 5-20 second was slightly shorter than the reagents A, B and C; however, no mortality was observed in any group except with the reagents B (concentration: 20 g/L, immersion time: 5 sec) and C (12 g/L, 10 and 20 sec) that showed negligible mortality of 3.3%. Higher concentration and longer treatment with the reagents resulted in longer recovery time of the detached abalone. Although abalone exposed to the reagents needs slightly longer time to recover than that to OTC, the exfoliation effect is much similar. These results indicate that the commercial exfoliating reagents can replace OTC to detach abalone, though they need to be cautiously handled.

유카추출물과 didecyldimethylammonium chloride를 주성분으로 하는 살균소독제 아쿠아 팜세이프의 어류병원체에 대한 살균 효과

서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jeon ),황지연 ( Jee Youn Hwang ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ),이성민 ( Sung Min Lee ),이은혜 ( Eun Hye Lee ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2

In this study, the disinfectant efficacy of Aqua farmsafe®, composed of didecyldimethylammonium chloride (DDAC) and yucca extract was evaluated against Salmonella typhimurium and fish pathogens. Determination of the anti-microbial or anti-viral efficacy of the disinfectant was based on Animal, Plant and Fisheries Quarantine and Inspection Agency Regulation No. 2011-26, Korea. Anti-bacterial efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4℃. Aqua farmsafe and test bacteria or virus were diluted with distilled water (DW), standard hard water (SW) or organic matter dilution (OM) according to treatment condition. Under the our results, disinfectant efficacy of Aqua farmsafe® possesses 30~40 fold against fish pathogens including bacteria and virus compared to that on animal pathogenic bacteria, S. typhimurim. As the efficacy of Aqua farmsafe® against fish pathogen was investigated in vitro, a controlled field trial is required to determine whether the use of Aqua framsafe® will be able to reduce fish diseases.

Edwardsiella tarda의 특이 Bacteriophage와 Bacillus subtilis KM-1혼합액이 Edwardsiella tarda 에 미치는 항균효과

백민석 ( Min Suk Baek ),황요셉 ( Yo Sep Hwang ),최상훈 ( Sang Hoon Choi ) 한국어병학회 2013 한국어병학회지 Vol.26 No.3

The present study was performed to investigate an antibacterial activity of specific bacteriophage (phage) and Bacillus subtilis KM-1 (B. subtilis) mixture against Edwardsiella tarda (E. tarda). An appropriate number of phage showing the most effective antibacterial activity was 2×105 PFU/㎖ with 1×10 CFU/㎖ of B. subtilis 36 h post incubation. On the other hand, B. subtilis showed a dose dependant manner in inducing antibacterial activity in the presence of phage (2×105 PFU/㎖). The phage and B. subtilis mixture showed higher antibacterial activity against E. tarda than phage or B. subtilis only. These results suggest that the phage and B. subtilis mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.

방류용 수산종묘의 수산생물 병원체 검출 동향 (2009~2012)

조미영 ( Mi Young Cho ),원경미 ( Kyoung Mi Won ),한현자 ( Hyun Ja Han ),김현정 ( Hyeun Jeong Kim ),지보영 ( Bo Young Jee ),김석렬 ( Seok Ryel Kim ),이순정 ( Soon Jeong Lee ),김진우 ( Jin Woo Kim ),박명애 ( Myoung Ae Park ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2

Hatchery-reared seeds provides a key source of animal protein for human consumption and restocking for fishery management. For stock enhancement program, we have inspected the hatchery-reared seeds of 33 species in 2009, 44 species in 2010, 43 species in 2011 and 46 species in 2012 for legally designated diseases. Results showed that abalone was the most abundant in the marine species group and then sea cucumber, olive flounder, rockfish and swimming crab were followed. Crucian carp was the most abundant and then mandarin fish, Korean bullhead, melanian snail and Chinese mitten crab were followed in the freshwater species group. The number of inspection for black sea bream, rock bream, scorpionfish, black scraper, and eel has continuously decreased for four years. The inspection for flathead mullet has been carried out only in 2009. The total number of inspection cases for eight pathogens in this study were 8,476 and disqualification cases were 56 (0.67%) by detection of aquatic animals pathogens such as koi herpesvirus, white spot syndrome virus, red sea bream iridovirus or viral haemorrhagic septicemia virus.

우리나라 양식 강도다리, Platichthys stellatus에서 분리된 Photobacterium damselae subsp. piscicida의 특성

조영아 ( Young Ah Cho ),한현자 ( Hyun Ja Han ),문희은 ( Hee Eun Mun ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ),김진우 ( Jin Woo Kim ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2

Starry flounder, Platichthys stellatus (body length 4.4 ± 0.51 ㎝) that became sick during an outbreak of disease at mariculture facilities at Ulsan, Korea in August of 2012, were examined to identify the cause of the disease. Diseased fish didn`t show a unique sign, but the oxidase-positive and gram negative rod was isolated from moribund fish. The bacterium was revealed as Photobacterium damselae subsp. piscicida by biochemical analysis and sequence analysis of the 16S rRNA and capsular polysaccharide (CPS) genes. The isolates (AD5) was carrying susceptible to ofloxacin and gentamycin and showed high growth value at 18℃ and 25℃ compared to four other P. damsela strains.

In vitro와 in vivo에서의 온도에 따른 스쿠티카충 성장의 수리 모델

오춘영 ( Chun Young Oh ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2

Population growth equation of scuticociliate Miamiensis avidus was obtained from the experimental results of in vitro culture condition to estimate the growth rate and carrying capacity from the growth equation. In addition, intraperitoneal infections into olive flounder Paralichthys olivaceus were carried out into 2 different conditions: different concentrations of M. avidus in same water temperature and same concentration of M. avidus in different water temperatures. Olive flounder mortality was threshold dependent with both the temperature and M. avidus density parameters. In this paper, we propose a mathematical model to study M. avidus growth in olive flounder based upon the interactions between parasite and host. The mathematical model was logistic growth differential equation (1.2). The parameters were found with Matlab program through the Levenberge-Marquardt method. In theorem, equilibrium values between the infected fish population and dead population could found. Our equilibrium points were a stable equilibrium and an unstable equilibrium. From the equation (1.6), it was possible to predict the amount of cumulative mortality of olive flounder along with the time after M. avidus infection.