한국어병학회 회칙 ( 한국어병학회지 창간호 , 1988 . 5 )

학회자료 한국어병학회 1988 한국어병학회지 Vol.1 No.1

향어에서의 oxolinic acid 흡수와 배설속도

정준기(Joon Ki Chung),정현도(Hyun Do Jeong),하재이(Jai Yi Ha),허민도(Min Do Huh) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

The absorption and excretion times of oxolinic acid(OX) used in farms as new aquatic antibiotics commonly were evaluated with determination of the effects of water temperature and feeding to parameters by using the bioassay technique. On the same time, antibacterial activity and the complex formation of oxolinic acid with serum proteins of two different fishes were compared to those oxytetracycline(OTC). With more than 10 times lower MIC values than those of OTC in the strains among 13 analyzed fish pathogens. OX did not show the decresed antibacterial activity by the binding of serum proteins in carp and tilapia. It implies more powerful potential of OX as aquatic medicine OTC. The serum concentration of OX after different administrations the oral, i.m., i.v and dipping methods were compared. The higher beginning concentration in serum and faster excretion times were obserbed in i.m. and dipping methods respectively. In the oral and i.m. administration, peak serum concentration after 24-48 hrs and slow excretion times demonstrated in both methods. These pharmacokinetic characteristics similar at 30℃ and 20℃ water temperature conditions, however, beginning serum concentration of OX in fish dipped in 50㎎/ℓsol after starvation for 2 wks was appeared lower than those of fed fish. It suggests the importance of biological condition of the gill or skin for absorption of antibiotics after dipping administration.

아질산의 만성중독증에 의한 참장어의 병리조직학적 연구

전세규(Seh Kyu Chun),양한춘(Han Choon Yang) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

The research was carried out to examine the chronic toxic effects of nitrite on the Japanese eel, Anguilla japonica by neans of histological observations. Young eel, 10.8g mean body weight. were exposed to 6 different concentrations of nitrite(1, 5, 10, 20, 30 and 40ppm) for 10 weeks. Each concentration was treated under 5 different levels of pH(5.5, 6.0, 6.5, 7.0 and 7.5) and each of these treatment was tested at 2 different temperature regimes(25℃ and 30℃). Proper concentration of nitrite was made by NaNO₂ and proper pH levels were made by the combinations of 0.1M KH₂PO₄ and 0.1M NaHCO₃. Histopathological test of gill tissues were made along with the test of the formation of thrombocystes and chloride cells on the gill filaments. At the lower pH levels, mucus secretion from the gill was incrased as the nitrite concentration increased. As the level of nitrite increased the number of chloride cells on the gill filament were decreased. Most of the remained chloride cells were observed only at the terminar part of the gill filament at 40ppm of nitrite. Degeneration of gill tissues were observed when nitrite levels were over 10ppm along with detachement and sweption of the epithelial cells of the gill lamellae. Shrunken gill lamellae and formation of thrombosis in the capillaries of gill lamellae were also observed. When temperature goes higher at the higher level of nitrite, necroses in the gill lamellae was increased. At the lower than 10ppm of nitrite, degeneration of gill lamellae was occured at the beginning of the test period but regenerated later. Negative effects of nitrite on the growth of young eel was started between 5∼10ppm at the pH level of 7.0 and 7.5. Thrombosis formation were also started at this level. The safety concentration of nitrite at the pH levels of 7.0 and 7.5 on the small eel seems to be 1ppm. Thrombosis and gill lamella detachment and necrosis in the gill capillaries were not observed at this level. Chloride cells were appeared the whole part of the gill filament.

월동장의 참동에서 분이한 비브리오균에 대하여

박수일,최혜승,윤장근 한국어병학회 1988 한국어병학회지 Vol.1 No.1

越冬實驗中인 참돔 病魚에서 病原菌을 分離하여 그 性狀을 比較檢討하였다. 그 結果, 原因菌은 生物學的, 生化學的諸性狀으로부터 Vibrio sp.로 同定되었다. 日本에서 報告된 참돔 由來의 Vibrio sp. (第III群)와는 重要性狀에서 差異가 났다. 分離由來가 다른 Vibrio sp. (第II群)와는 여러 性狀에서 一致하므로 低溫에서의 發育能이 差異가 나는 生物型으로 생각되었다. Three strains of pathogenic bacteria isolated from diseased red sea bream at a culture farm in Gyoung sangnam-Do during the midwinter of 1987 were studied taxonomically. Based on their biological and biochemical characteristics, they were identified as Vibrio sp., They exhibited very similarities to group II (Kusuda et al.,1979) except that the former can grow at lower temperature.

양식넙치로 부터의 Lysozyme 정제와 어류병원성 세균에 대한 정균작용

전세규(Seh Kyu Chun),박수일(Soo Il Park),김진우(Jin Woo Kim) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

Lysozymes were isolated and purified from various organs of cultured flounder by using chitin-coated cellulose column chromatography. The molecular weights of them were compared with each other in 15% SDS-PAGE gels. The result showed that all lysozymes isolated from various organs of flounder had the same molecular weight of about 14000. To clarify the role of lysoryme as a body defence, the antibacterial activities of flounder lysozyme on seven bacterial pathogens, five Gram-negative and two Gram-positive species, were investigated. The lysozyme had substancial antibacterial activity on four strains, two Gram-negative and two Gram-positive species. These suggest that flounder lysozyme plays a role in body defence against both Gram-negative and Gram-positive bacterial pathogens.

Aeromonas hydrophila 에 대한 비단잉어의 면역반응과 수산약제의 영향

지보영(Bo Young Ji),박수일(Soo Il Park) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

This study was carried out in order to investigate the immunosuppressive factor and immune response of color carp. The protection and serum antibody production of juvenile color carp aganist Aeromonas hydrophila were investigated on the effect of temperature differences and injected several aquatic drugs, i.e. Hydrocortisone, Oxytetracycline, Chloramphenicol and Ascorbic acid. The fish were injected intraperitoneally with 1㎎/fish of HKC and FKC at three different temperature conditions as 16℃, 24℃, and 32℃ respectively. There were induced better protection and serum antibody production in the fish which had been kept at 24℃ than in the fish which had been kept at 16℃ and 32℃. The FKC immunized fish were followed 24 hrs later with intraperitoneal injection of 40㎎/㎏ body weight of Hydrocortisone, 60㎎/㎏ body weight of Oxytetracycline. 60㎎/㎏ body weight of Chloramphenicol and 30㎎/㎏ body weight of Ascorbic acid, respectively. The control fish were injected PBS only. The fish given the above aquatic drugs reduced serum antibody production level and protection rate when compared to control fish. As the results, immune response of juvenile color carp immunized FKC at 24℃ was more effective than 16℃ or 32℃ and immune response of juvenile color carp injected several aquatic drugs which was seemed to be immunosuppressive factor.

챠넬메기의 metallothionein cDNA 유전자의 cloning 및 그 특성에 관한 연구

이인정(In Jung Lee),송영환(Young Hwan Song) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

Metallothionein is an essential and common protein to regulate the intracellular concentration of heavy metals, which exist in most organisms from bacteria to vertebrates. Although the detailed function of metallothianein has not been fully identified until yet, it may be involoved in the cellular protection against the heavy metal toxicity and in the global regulation of several other genes and the expression of metalloproteins. We have cloned the full cDNA clone of metallothionein gene in Channel Catfish by Reverse Transcriptase-Polymerase Chain Reaction(RT-PCR) starting from poly(A)-containing mRNAs. All PCR fragments have been subcloned into EcoRV site of pBluescript SK+ and dT-tailed at Smal site of pUC19, then PCR products are recovered by the double digestion of recombinant plasmids wiht EcoRI and HindIII, which are adjacent to EcoRV site in multicloning sites or by rapid PCR screening. The nucleotide sequence analysis of pMT150(one of the PCR clones) showed high homology with several other piscine metallothionein cDNA genes.

틸라피아에 있어서 Edwardsiella tarda 의 실험적 감염에 관한 연구

전세규(Seh Kyu Chun),최동림(Dong Lim Choi),정준기(Joon Ki Chung),김광희(Kwang Hee Kim) 한국어병학회 1992 한국어병학회지 Vol.5 No.2

Experimental infection method for Edwardsiellosis was studied to imitate histopathologically natural infections in the Tilapia Oreochrimis niloticus. Prior to the bacterial challenge, the intestine of tilapia was damaged by 0.2㎖ of 30% hydrogen peroxide introduced through a silicon tube which was inserted 4 to 5cm into the intestine from anus. E. tarda was mixed a 10% Gum arabia and administered into the stomach by a cannula 20 hours after the hydrogen peroxide treatment. Bacterial doses used were from 4.2 ×10^6 to 6.4 ×10^8 CFU/fish. Fish 72 hours after the challenged showed swollen and erosious lesion and focal necrosis with bacterial-eaden inflammatory cell in the Liver, Spleen, Kidney. Fish 96 and 120 hours after bacterial challenge were moribund showing swollen Liver, Spleen, Kidney and focal accumulation of macrophages and production of granulomas in the infected lesions.

틸라피아의 에드와드병에 대한 백신과 Adjuvant 의 효과

이주석(Joo Seok Lee),박수일(Soo Il Park) 한국어병학회 1992 한국어병학회지 Vol.5 No.1

A formalized bacterin(FKC) of Edwardsiella tarda, conjugated with/without Freund`s complete adjuvant(FCA), Freund`s incomplete adjuvant(FIA) and potassium aluminium sulfate(PAS), was administered by intraperitoneal injection to tilapia respectively. The tilapia were reared under indoor recirculating filter system. There were agglutinin titer increment in the tilapia which were administered not only FKC bacterin but also adjuvanted vaccines. But the producing time of the highest level of antibody in the immunized tilapia was different among vaccine preparation, i. e. FKC + FCA group and PAS + FKC group are between the 2nd and the 4th week, and FKC alone group is the 2nd week. In the challenge experiment with 2.5× 10^7 CFU/㎖ or 2.5×10^8 CFU/㎖ of E. tarda T1123 to the tilapia immunized with vaccines, RPS above sixty was recorded both challenge dose in the tilapia which were 3 weeks after immunization with FKC+FCA, PAS+FKC and FKC alone, and the former dose 8 weeks after immunization with FCA+FKC and PAS+FKC. There were some resistance enhancement against E. tarda in the tilapia which were injected with adjuvant alone than that of control. As the results, the FCA or PAS adjuvanted vaccine is effective to sustain the defensible period against edwardsiellosis.

어류혈청의 보체에 의한 살균작용

유병화(Byoung Hwa Yoo),전세규(Seh Kyu Chun),박수일(Soo Il Park) 한국어병학회 1992 한국어병학회지 Vol.5 No.1

In order to know the defense mechanism of fish, bactericidal activity was examined into the sera of carp Cyprinus carpio, tilapia Oreochromis niloticus and flounder Paralichthys olivaseus. Each examined normal serum of fishes showed the bactericidal activity against avirulent Escherichia coli but it wasn`t appeared against virulent Edwardsiella tarda. When the normal serum of each fish was treated with zymosan, it lost the bactericidal activity completely. After addition of EGTA into the normal serum of each fish, the sera of tilapia and flounder still exhibited the bactericidal activity but the serum of carp lost it. In the presence of specific antibody and complement, bactericidal activity of each antiserum was exhibited high level with in one hour incubation. On the other hand, heat inactivated antiserum showed bacteriostatic reaction. From the above results, although the activity of alternative or classical pathway of each fish complement is different, it is an important function in fish defense mechanism.