백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

관해유도 항암요법을 받는 백혈병 환자에서 진균 감염증의 예방 : 무작위 배정법과 이중 눈가림법에 의한 Fluconazole과 Nystain의 효과에 관한 다기관 공동연구 A Randomized, Double-blind, Multicenter Trial to Compare Fluconazole with Nystatin

최강원,오명돈,배현주,백경란,박선양,김병국,신완식,강문원,진종률,박종원,김춘추,김동집,한지숙,민유홍,이선주,고윤웅 대한화학요법학회 1993 대한화학요법학회지 Vol.11 No.2

Fluconazole의 진균 감염증 예방 효과와 안전성에 관하여 3개 대학병원에서 관해유도화학요법을 받는 급성 백혈병환자를 대상으로 무작위 배정법과 너도나도 누가림법에 의하여 연구하였다. 모두 62명의 환자에게 fluconazole(100㎎ bid) 또는 nystatin(1,000,000IU/day)을 무작위로 투여하였다. 투약은 관해유도화학요법과 같은 날짜에 시작하여 호중구수가 1,000㎣이상으로 회복되거나 진균 감염증이 확인되거나 의심되어 Amphotericin-B를 시작하거나, 약과 관련된 부작용이 나타날 때까지 계속하였다. 진균 colonization은 fluconazole군에서 감소하였으나 nystqatin군에서는 증가하였다, 표재성 진균감염증으로 nystatin군에서 C. albicans 진균혈증 1례와 C.parasilosis 진균혈중 1례가 발생하였다. 경험적 항진균요법으로 Amphotericin-B를 투여한 경우는 fluconazolerns 34명중 7례(21%), nystatinrns 28명중 10례(36%)였다(p<0.05). Fluconazole군과 nystatin군 사이에 부작용이나 사망률에 차이는 없었다. 결론적으로, fluconazole은 관해유도화학요법을 받는 급성 백혈병환자에서 진균의 colonization을 줄이는데 효과적이고 안전한 항진균제이다. We made a randomized, double-blind, multicenter trial to compare the efficacy and safety of fluconazole with nystatin for prevention of fungal infections in patients with acute leukemia. Sixty-two adult undergoing remission induction chemotherapy for cute leukemia were enrolled. Patients were randomly assigned to receive either fluconazole (100㎎ bid) or nystatin(1,000,000IU×6/day) with corresponding placebo. The study drug was started in initiation of chemotherapy and continued until recovery of neutrophil counts(>1,000/㎣), development of proven or suspected invasive fungal infection, or the occurrence of drug-related toxicity. Fungal colonization decreased in fluconazole(F) group, however increased in nystain(n) group. Superficial fungal infection occurred in 1 of 34 F group, whereas invasive fungal infection developed in 3 of 28 N group. Empirical amphotericin-B therapy was given in 7 of 34(21%) F group and 10 of 28(36%) N group(p>0.05). The incidence of drug-related side effects and overall moratlity were similar in both study groups.

자발성 세균성 복막염을 일으킨 그람음성간균의 3세대 세팔로스포린 내성률 변화

방지환,송경호,박주경,박완범,김성한,김홍빈,김남중,오명돈,김의종,이효석,최강원 대한감염학회 2007 감염과 화학요법 Vol.39 No.3

The third generation cephalosporin is widely used in treatment of spontaneous bacterial peritonitis (SBP). Resistance to the third generation cephalosporin was associated with poor outcome in patients with SBP. Thus it is necessary to know the changes in resistance rate. We planned to investigate retrospectively on resistance rate of the third generation cephalosporin of gram negative bacteria isolated in patients with SBP, who visited Seoul National University Hospital between 1998 and 2006. The Clinical and Laboratory Standards Institute guidelines were applied for antibiotic susceptibility test. A total of 269 consecutive episodes of clinically and microbiologically proven SBP was identified during the study period and 209 cases were caused by gram negative organisms. Among 209 isolates, 22 (10.5%) showed resistance to the third generation cephalosporin. The prevalence of resistance was decreasing during the study period (P=0.014).

Mycophenolic Acid와 Rapamycin이 흰쥐 사구체 혈관간세포증식과 세포외기질 생성에 미치는 영향

김명수,박제현,하헌주,허규하,서지연,김유선,김혜진,박기일 이화여자대학교 약학연구소 2004 藥學硏究論文集 Vol.- No.14

Background: Excess proliferation and extracellular matrix (ECM) accumulation of mesenchymal cells such as vascular smooth muscle cells (VSMC) and glomerular mesangial cells cause chronic allograft nephropathy showing transplant vascular sclerosis and glomerulosclerosis. Mycophenolic acid (MPA) and rapamycin (RPM) are well known as strong inhibitors of VSMC proliferation, but their effects on the glomerular mesangial cells are not yet clearly understood. This study examined the effects of MPA or RPM on PDGF-induced proliferation and ECM accumulation in rat glomerular mesangial cells. Methods: Mesangial cells isolated from the glomeruli of Sprague-Dawley rats were cultured with DMEM containing 20% fetal bovine serum. Growth arrested and synchronized cells were administered with test drugs (MPA10 nM-10μM, RPM 0.1 nM-1μM) before the addition of PDGF 10 ng/mL. Cell proliferation was assessed by [³H]thymidine incorporation, collagen by [³H]proline incorporation, and fibronectin, ERK, and p38 MAPK by Western blot analysis. Results: PDGF increased mesangial cell proliferation by 4.64-fold. Compared to stimulated control, MPA above 500 nM and RPM above 10nM showed a significant inhibitory effect in a dose-dependent manner. The IC_(50) of MPA and RPM against PDGF-induced mesangial cell proliferation were around 500 nM and 100 nM, respectively. The collagen synthesis was also inhibited by MPA and RPM, but the fibronectin secretion was inhibited by MPA alone. The proliferation of mesangial cell correlated with activation of ERK and. p38 MAPK. MPA, but not RPM, inhibited ERK and p38 MAPK activation. Conclusion: This study demonstrated that MPA and RPM significantly inhibited PDGF-induced proliferation and ECM production in rat glomerular mesangial cells. The inhibitory effects of MPA, but not RPM, are correlated with ERK and p38 MAPK.

지도부딘 투여력이 없는 Human Immunodeficiency Virus Type 1 감염환자에서 지도부딘 내성 발현율

김남중,최은숙,김의석,이대호,최영주,방지환,오명돈,최강원 대한감염학회 2001 감염 Vol.33 No.1

Backgroud : Drug resistant virus has been seen with all effective antiretroviral drugs in current practice. The isolation of resistant virus from subjects is associated with more rapid clinical outcome. The presence of resistance-conferring mutations in a patient in whom therapy is foiling indicates that other antiretroviral drugs should be considered. So, the identification of drug resistant Human Immunodeficiency Virus (HIV-1) mutations is of increasing importance. Zidovudine monotherapy for HIV-1 infection has been widely used in Korea since 1990. So, the prevalence of zidovudine resistant virus has been expected to be high. The purpose of the present study is to determine the prevalence of zidovudine resistant HIV-1 among zidovudine-naive Korean patients. Methods : We tested drug susceptibility of HIV-1 isolated from 19 zidovudine-naive Korean patients. We amplified a portion of gene reverse transcriptase of HIV-1 by RT nested PCR method. The susceptibility of HIV-1 to zidovudine was determined by sequencing the DNA base pairs of gene reverse transciptase. Results : Two (10.5%) of 19 HIV-1 isolates from zidovudine naive patients showed resistance conferring mutations to zidovudine. Two (2.1%) of 95 codons analysed from zidovudine naive patients showed mutations conferring resistance to zidovudine. Conclusion : The prevalence of zidovudine resistant HIV-1 was 10.5% among zidovudine-naive Korean patients. (Korean J Infect Dis 33:40~45, 2001)

심부전 백서의 대동맥과 폐동맥에서 보이는 C형 이뇨단백에 대한 이완반응의 이상

김철호,김광일,조영석,조주희,오병희,이명묵,박영배,최윤식,이영우 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

금성산의 곤충상에 관한 연구

김재근,주명칠 동신대학교 환경연구소 2000 환경연구 Vol.5 No.1

A faunistic study on the terresterial insects (except soil insects) of Galma Mt. was carried out during march to November in 2000 year. The area is located in the boundary between Naju-si and Kwangjoo-si province of the central part of Chonnam which is composed of the main peak(alti. 450m) and other peakes. The results obtained as follows; 1. The terrestrial insects collected and identified from this area are composed of 86 species of 49 families of 10 orders from Keumseong Mt. in 2000 year. 2. The collected species are many of ordonata, coleoptera, diptera and lepidoptera. 3. The collected families are many of coleoptera, diptera and lepidoptera. 4. The collected individuals are very many of Cicadalla virids of cicadellidae of homoptera.

혈액에서 분리된 세균의 항생제감수성 : Vitek System을 이용한 최소억제농도측정 Minimal Inhibitory Concentration Determined by Vitek System

김의종,김선주,신보문,박명희,조한익,김상인 대한화학요법학회 1988 대한화학요법학회지 Vol.6 No.1

Minimal inhibitory concentrations of total 148 strains isolated from blood culture at Seoul National University Hospital were determined by Vitek System. 90% MIC of gram-negative bacteria against amikacin was 2-16㎍/㎖, and those against beta-lactams was > 16㎍/㎖ in general, Except Salmonella spp,. 90% MIC against chloramphenicol and tetracycline was >8㎍/㎖. Range of 90% MIC against vancomycin was ≤.5-4㎍/㎖ in case of garm-positive bacteria, except Enterococcus spp. which showed 90% MIC as >16㎍/㎖. Against ampicillin, 90% MIC of Staphylococcus spp. was >16㎍/㎖, but those of Streptococcus spp. was ≤.25-2㎍/㎖. Streptococcus viridans group showed 90%MIC against penicillin as ≤.03㎍/㎖. Susceptibility of gram-negative bacteria was generally 98% against amikacin, and those of gram-positive bacteria was 80% against vancomycin. 34% of Staphylococcus spp. was resistant of oxacillin. These results may be useful for the primary selection and the dosage determination of antibiotics in the treatment of patients with bacteremia.

食用造景樹種의 利用形態 및 活用方案에 관한 硏究 : 광주·나주 아파트단지를 사례로 For example a collective Apt. area in Kwang-ju, Na-ju

주명칠,김재근,이선자 동신대학교 환경연구소 2000 환경연구 Vol.5 No.1

본 연구는 우리나라의 식용조경식물의 이용증대 및 식용식물을 활용하는데 기초 자료를 제공하고자 광주와 나주지역의 아파트를 대상으로 지역별ㆍ평형별 식재현황을 조사분석 하였으며 식용조경식물의 이용실태를 분석한 결과는 다음과 같다. 5개 아파트내의 식재된 목본성 조경식물은 25과 39속 48종 10변종 2품종으로 구성되어 있는데, 그중 식용조경식물은 9과 11속 13종 3변종 1품종으로 전체수종 중 총 28%를 차지하였다. 식용조경식물의 지역별 식 재현황은 광주지역에서 15종, 2,097주로 전체의 87.89%이다. 그중 곰솔이 15.21%, 잣나무 12.20%, 대추나무 11.82% 순이다 그리고 나주지역의 경우는 7종, 289주로 전체의 12.11%이고, 그중 곰솔 5.87%, 사과나무 2.47%였다. 아파트 평형별 식용조경식물의 특성을 살펴보면, 49 ~ 62평(746주)은 24 ~ 33평(1038)보다 수량은 적었지만 종의 다양성(49~ 62평 11종, 24 ~ 33평 9종)은 49 ~ 62평이 더 높았다. 조사지내의 식용조경식물의 침엽수종과 활엽수종간의 비율은 45 : 55 이였으며 상록수 대 낙엽수의 비율은 41 : 59이고 교목 대 관목의 비율은 67 : 33 이였다. 출현횟수는 4회가 은행나무ㆍ소나무, 3회가 감나무였다. 1회의 출현빈도가 58.82%로 가장 많았으며, 2회가 23.53%, 4회가 11.76%, 3회가 5.88%였다. 식용조경식물의 이용부위별로 보면 과실은 15종, 꽃 7종, 잎 5종, 수피 4종 순이였다. 출현횟수는 4회가 은행나무ㆍ소나무, 2회가 잣나무ㆍ곰솔 등이였다. 1회의 출현빈도가 꽃 57%, 과실 53%, 수피 25%,잎 20% 순으로 많았으며, 2회가 수피 50%, 잎 40%, 꽃 29%, 과실 27%, 4회가 잎40%, 수피 25%, 꽃 14%, 과실 13%, 3회가 과실 7%였다. 광주ㆍ나주아파트 식용 조경식물의 식재현황은 전체수종 60종에 비해 총 17개 수종으로 전체의 28%를 차지하였으며, 식용조경수종 17개 수종 중 일부수종이 대부분을 차지하였다. 이러한 결과 식용조경수종의 식재에 대한 전반적인 검토가 필요하며, 식용조경식물의 보급화와 실용화가 이루어져야 한다. This study is for data base of korean edible landscape woody plants. The aim of research is use increase and application plan of edible landscape woody plants. For activating edible landscape woody plants in Apts researched and analysed by the location and the scale of Apt is 3 Apt in Kwang-ju and 2 Apt in Na-ju. The result are followed. The investigated area have landscape woody plants, 60 taxa with 25 families, 39 genera 48 species, 2 varieties. Edible landscape woody plants that are 60 taxa with 9 families, 11 genera, 13 species, 3 varieties, 1 forms, occupied 28% of the all. Regionally, the planting situation of edible landscape woody plants in Kwang-ju is 15 species, the number of landscape woody plants is 2,097 plants. That is 87.89% of the investigated area. Pinus thunbergii 15.21%, Pinus koraiensis 12.20%, Zizyphus jujuba var. inermis 11.82% in order. In Na-ju is 7 species, 289 plants that occupies 12.11%. Pinus thunbergii is 5.87%, Malus punila var. dulcissima is 2.47%. Regarding as the characteristics of edible landscape woody plants the size of Apts, in the 49 ~ 62pyong Apt (746 plants) are less number of trees than the 24~33pyong (1,038 plants). But the variety is more than the 49 62pyong Apt. Ratio of niddle and broad leaved in edible landscape woody plants researching sections is 45 : 55. That of evergreen to deciduous is 41 : 59, that of tree to shrub is 67 : 33. Frequency of appearance is four times in Ginkgo biloba and Pinus densiflora, three times is Diospyros kaki. One frequency is most of 58.82%, two is 23.53%, four is 11.76%, three is 5.88%. In part of use edible landscape woody plants, 15 species for fruits, 7 species for flowers, 5 for leaf, 4 for bark in order. The planting situation of edible landscape woody plants in Kwang-ju and Na-ju occupy 28% that is 17 species of 60 species plants that is all. Though edible landscape woody plants is 17 species some species are most. The result shows that we need overall investigation edible landscape woody plants species, suply and preacticability of edible landscape woody plants.

소나무로부터 배출되는 모노테르펜 구성비의 수령별 특성 연구

김조천,홍지형,주명칠,전진,김기준 동신대학교 환경연구소 2002 환경연구 Vol.7 No.1

소나무로부터 직접 배출되는 주요한 모노테르펜 물질로 α-pinene, camphene, myrcene, β -pinene, α-phellandrene, α-terpinene, d-limonene, p-cymene, β-phellandrene 등이 확인되었다. 7년생 소나무에서 직접 배출되는 모노테르펜 물질의 구성비를 분석한 결과 β-pinene이 67.0%로 가장 높았고, 22년생 소나무 경우에 β-phellanone이 37.4%로 가장 높았으며, 32년생 소나무는 α-pinene 구성비가 약 42.9%로 가장 높았다. 소나무는 수령에 따라서 구성비에 큰 차이를 보였으며, 소나무 수령이 높을수록 α-pinene이 구성비가 높았다. 소나무의 수령별 α-pinene과 β-pinene의 비율(α-pinene/β-pinene)을 비교한 결과, 7년생 소나무의 경우 0.16~l.15 범위로 나타났고, 22년생 소나무의 경우 0.64~2.47로 나타났으며, 32년생 소나무의 경우 0.50~18.47 범위로 나타났다. α-pinene과 β-pinene의 비는 수령이 높아질수록 좀 더 높은 값을 보였고, 동일한 수령일지라도 나무에 따라서 차이를 보였다. The major monoterpenes emitted from the Pinus densiflora were α-pinene, camphene, myrcene, β-pinene, α-phellandrene, α-terpinene, d-limonene, p-cymene and β-phellandrene. As a result of comparison of monoterpene composition by tree age for P. densiflora, it was found that β-pinene was a dominant monoterpene for 7-year-old pine(67%). β-Phellandrene(37.4%) and α-pinene(46.1%) were most abound for 22-year-old pine and 32-year-old pine respectively. The ratio of monoterpene composition varied considerably with tree age for the pine, and the percentage of α-pinene composition increased with tree age. The ratio of α-pinene and β-pinene(α-pinene/β-pinene) increased with tree age, and varied tree to tree.