간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현

김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5

Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.

스포츠에 의한 성장기 요추추체종판 장해 분석

김민섭,이종영,이충영,이승훈,임완기,강은균,이종호 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

Apophyseopathy in human growing-up period is divided in three parts, early stage, advancing stage, and ending stage which are analyzed by X-ray test. The tuber of kantenabtrennung, schmorl and rear oxhorn dissociation are one of the these barrier process. We assume that the fracture of apophysis by overusing is the main cause of these barriers.

건축공사시방서에서의 KS규격의 국제인증방안에 관한 연구

김경식,이재섭,김용수,이규진,조용훈,최완철,전재열,이현수,구재동,이기환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The purpose of this research is make out construction specifications for International Construction. Using off-shore products incur cost increase and longer delivery term of construction. and inconvenient on International Construction. Expand the range of additional local products conforming to performance. Life Cycle Cost. maintainability. operability. user's acceptability, etc.. And this study is suggest the standard formats and the techniques for construction specification.

골수이식 후 골밀도 및 골대사의 변화 : 2년 추적관찰 2-Year Prospective Study

이원영,강무일,오은숙,오기원,한제호,손현식,윤건호,차봉연,이광우,손호영,강성구,신완식,민우성,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

소라, Turbo cornutus SOLANDER의 棲息場 環境과 集團成長에 關하여

李定宰,李祺完,李廷烈 濟州大學 臨海硏究所 1978 해양과환경연구소 연구논문집 Vol.2 No.-

1977년 6月부터 12月까지 西歸浦 沿岸에 位置한 숲섬,甫木里,法還里의 3個 養成場을 對象으로 海洋基礎生態學的 要因 및 標識放流한 소라의 成長과 移動등을 調査하였다. 1. 各 養成場의 水溫 및 比重의 垂直的 變化는 보이지 않았고 숲섬 養成場이 多少 低溫을 나타냈다. 2. 숲섬 養成場의 海低地型은 B,C형태이고 甫木里 養成場은 A形態,甫木里 養成場은 C, D形態가 主로 構成되어 있다. 3. 各 養成場의 流速은 숲섬 養成場이 가장 빨라 4∼20 ㎝/sec였고, 法還里 養成場은 1∼10㎝/sec로 緩慢한 流速을 보였다. 4. 西歸浦 沿岸의 吸收係數는 0.218∼0.329(평균 0.283)이였고 숲섬, 法還里, 甫木里 順으로 작았다. 5. 調査된 海藻相 中 各 養成場別 優點種은 共히 모자반, 감태, 석회조, 클라도포라 등이였으며 특히 法還里 養成場은 모자반이 優勢하게 分布했다. 6. 標識放流한 소라의 成長度는 6月부터 10月까지 빠른 傾向을 보였고,養成場別 成長速度는 甫木里,숲섬,法還里의 順이었다. 7. 標識放流期間 동안 具殼 新生部에 形成된 棘數는 2∼3個가 가장 많았다. 8. 소라의 移動거리는 各 養成場 共히 5∼25m의 範圍가 가장 높은 頻度를 보였다. Studies on the growth of topshell which had tagging liberated and ecological survey were taken from the three culturing topshell forms of the Seogwipo coast form June to December, 1977. Three culturing topshell farms are Supseom, Bomkri and Beophwanri coat. The culturing topahell farms are shallow with the mean depth 5∼8m, and vertical changes of water temperature and specific gravity in each arears were not appeared. The water temperaure of Supseom area was lower than the other areas. The compositions of submarine geological type in Supseom, Bomokri and Beophwanri area were B (Hole type) and C (crevice type) type, A (Rock type) type, and C and D (Sand type) type respectively. The Current speeds of each culturing farm area were in the range of 1∼20㎝/sec, and the current of Beophwanri culturing farm area was slower than the other areas. The absorption coefficient of Seogwipo coastal water in the range of 0.218~0.329 and the mean was 0.283. Fifty species of marine algae were identified. Major algal group of each culturing farms were Sargassum sp.,Ecklonia cava, Chladophora sp., calcareous algae. Particularly, Beophwanri culturing farm was abundant in Saragassum sp. Of the three culturing topshell farms increment in growth of topshell were showed rapid growth from June to October and noted that increment in growth of Bomokri was the highest. During the tagging liberated period, the number of spines on newly formed parts of shell was in the range of 0∼5 spines. The creeping distance was 5∼25m in each culturing topshell in all.

구인 peroxidase의 부분 정제 및 효소학적 특성 규명

이미영,김윤경,이기완 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

Peroxidase has been partially purified from earthworm, Lumbricus rubellus, using ammonium sulfate fractionation, Sephacryl S-200 gel filtration, CM-cellulose cation exchange chromatography and native-PAGE elution. Some of its enzymatic characteristics were examined. Earthworm peroxidase showed the characteristics of heat-sensitivity, and the enzyme activity was stimulated in the presence of various cations. The most available booster was CoCl₂, followed by ZnCl₂and KCl among cations used. The optimum pH for guaiacol oxidation of earthworm peroxidase was determined to be 6.0, and the Km values against guaiacol and H₂O₂were 1.25mM and 3.4 mM, respectively. When various compounds were tested as the possible substrates of the enzyme, o-dianisidine was used as the substrate. However, earthworm peroxidase could not oxidize esculetin and ferulic acid as substrates, suggesting the different characteristics of the enzyme from plant peroxidases. The opimum pH for veratryl alcohol and H₂O₂oxidation was determined to be 2.5 when lignin peroxidation activity was examined. The Km values for veratryl alcohol and H₂O₂were 0.02 mM and 0.13 mM, respectively.

제주대학 임해연구소 부근의 해조분포 및 식생

이기완 제주대학교 1974 논문집 Vol.6 No.-

저자가 1974년 3월부터 1975년 2월에 걸쳐 1년간 조사한 제주대학 임해 연구소의 해조분포 및 식생은 아래와 같다. 1) 채집된 해조는 모두 101종으로 녹조류 18종, 갈조류 36종, 홍조류가 47종이다. 2) 이 지역의 대표적인 해조군집은 ① Porphyra-GloiopeltiAssociation; ② Ishige Association; ③ Hizsikia fusiforme Association; ④ Sargassum Association; ⑤ Collaina Association으로 식물 사회학적인 분포로 보아 Hizkietum Taniputi에 속한다. The annual variation of marine algal flora including 101 species in all(18 species of Chlorophyta, 36 species of Phaeophyta, 47 species of Rhodophyta), was observed at Marine Laboratory of Cheju University near Seogwipo for one year from March 1974 to February 1975. Five typical algal association were observed at the observed station; 1) Porphyra Gloiopeltis Association; 2) Ishige Association; 3) Hizikia fusiforme Association; 4) Sargassum Association; 5) Corallina Association.

제주도 서귀포연안 식물성 플랭크톤의 계절변화 : 1.하계절 식물성 플랭크톤의 종의 분포와 양적조성

이기완 제주대학교 해양연구소 1979 해양자원연구소연구보고 Vol.3 No.-

This present paper deals with a study on the distribution of species, quantity and specific composition of phytoplankton at the Seogwipo coast of Jeju-Do, which were obtained in June-August 1979. Collected phytoplankton were 31 species totally, 16 species belong to genus Chaetoceros. 4 species to Rhitosofenia.2 species to Niizschia 2 species to dinoflagellate, and other 1 species to diatoms as follows. Chaetoceros afmis, Ch. didymus, Ch. didymus, v. anglica, Ch. didymus v. prototuberans. Ch. decipiens, Ch. compressus, Ch. debilis, Ch. distsans, Ch. pseudocrinitus, Ch. peruvianus, Ch. lorenzianus, Ch. borealia, Ch. weissflogii, Ch. radicans, Ch. anastomosans, Ch. sp.. Rhizosolenia stolterfothii. Rh. fragilissima, Rh. habetata, Rh. robusta, Nitzschia seriata, N. sp., Melosira sp.. Ditylium brightwellii. Thalasiothrix nitzschioides, Pleurosigma offine, Navicula sp., Ceratium fusus Skelletonema costatum, Peridinium pellucidum, Gymnodinium sp. The total individuel cells of phytoplankton were 510, Chaetoceros(248 cells, 48.63%), Rhizosolenia (156 cells, 30.59%). dinoflagellate(75cells, 14.7%), and other 1 genus of diatoms (31cells, 6.08%) in descending order. 18 species belong to 4 genera were typical species in the coastal water of Curoshio current, the species which appeared through a year were 2 species to 1 genus. 5 species to 4 genera were typical species of cold water.

에이즈 환자에서 발생한 조류형결핵균 뇌수막염 1예

이기덕,박완범,정혜승,강철인,김동민,김홍빈,오명돈,최강원 대한감염학회 2003 감염과 화학요법 Vol.35 No.5

에이즈 환자의 중추신경계 합병증으로 에이즈 바이러스 자체에 의한 합병증, 기회감염, 종양 등이 있다. 저자들은 전신긴장간대발작을 일으킨 31세 남자 에이즈 환자에 발생한 조류형결핵균 뇌수막염을 조직학적 및 미생물학적으로 증명하고, 항비결핵미코박테리아 약제를 고농도로 투여하여 치료하였다. 조류형결핵균 뇌수막염은 결핵성 뇌수막염으로 잘못 진단되면 치명적인 결과를 초래할 수 있으므로, 조직검사를 포함한 적극적인 검사가 필요하다. We report a case of Mycobacterium avium-intraceliuiarae meningoencephalitis found in a 31-year-old patient with AIDS and preexisting disseminated Mycobacterium avium-intracellularae infection. He had been taking highly active antiretroviral therapy and antimycobacterial medications when he developed generalized tonic-clonic seizure. Repeated workups including blood and cerebrospinal fluid examinations and magnetic resonance image (MRI) of the brain revealed nondiagnostic. The diagnosis was made by stereotactic brain biopsy of the nodule, which was detected 9 month after the first seizure attack in brain MRI. Acid-fast bacilli were observed on microscopic exam. Mycobacterium avium-intracellularae was isolated from the biopsy specimen.

동종골수이식 후 혈당 및 혈중 지질농도의 변화양상 및 관련인자

이원영,강무일,오은숙,오기원,손현식,윤건호,차봉연,이광우,손호영,강성구,신완식,민우성,김춘주 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.6

연구배경:골수이식은 비교적 젊은 연령의 환자들을 대상으로 하며 면역억제제의 사용기간이 비교적 짧으므로, 주로 고령의 만성 질환 환자에서 시행되는 고형장기이식에서와는 달리 당대사 및 지단백 대사에 있어서 많은 차이점이 있을 것으로 추측된다. 저자들은 전향적 연구를 통하여 골수이식 후 시기별로 혈당 및 혈중지단백 변화 양상을 알아보고 이와 관련된 임상인자들을 규명하고자 하였다. 방법:1998년 10월부터 1999년 8월까지 가톨릭대학교 성모병원에서 동종골수이식을 시행한 환자들 중 43명을 대상으로 이식 전 및 이식 후 1, 2, 3, 4주와 3개월, 6개월에 공복 혈당, 혈중 총 콜레스테롤, 중성지방, 고밀도지단백을 측정하여 골수이식 후 시기별 변화를 관찰하고 여러 임상인자들에 따른 차이를 알아보았다. 결과:1. 공복혈당은 골수이식 수 첫4주 동안 상승하였고 이후 감소하였으나 이식 6개월 시점의 평균 공복혈당은 이식 전보다 유의하게 높게 관찰되었다. 혈중 총 콜레스테롤은 이식 후 1주에 최고치를 형성하였고 이후 기저수준으로 회복되었다가 이식 수 3,6개월에 다시 증가하여 기저치에 비해 유의하게 높았다. 혈중 중성지방은 이식 후 1개월까지 유의하게 증가하였고 이후 감소하여, 3,6개월에는 기저치와 유의한 차이가 관찰되지 않았다. 혈중 고밀도지단백은 이식 후 2,3주에 이식 전보다 유의하게 감소하였고 이후 이식 전 수준으로 회복되었다. 혈중 저밀도지단백은 총 콜레스테롤의 변화와 매우 유사하였다. 2. 골수이식 후 6개월 시점에서 공복 혈당이 126㎎/dL이상인 환자는 7명(16%)이었다. 이들 환자들과 공복혈당 126㎎/dL 미만의 환자 36명을 비교한 결과, 공복혈당이 126㎎/dL 이상인 환자군에서 스테로이드 평균 투여량이 많았고 고밀도지단백이 유의하게 낮았다. 3. 이식편대숙주질환이 발생한 환자는 그렇지 않은 환자보다 이식 후 1, 2, 3주 및 6개월의 총 콜레스테롤이 더 낮았고, 3개월의 공복혈당이 더 높았다. 혈연골수이식 환자군은 비혈연골수이식 환자에 비해 투여된 스테로이드 용량이 더 적음에도 불구하고 이식 후 1, 2, 3주 및 6개월의 총 콜레스테롤 수치가 더 높았다. 스테로이드 고용량 투여군(하루 평균 7.5㎎기준)은 저용량군보다 이식 후 3개월 시점의 공복혈당이 더 높았으나 나머지 시점의 혈당, 혈중 지질농도에 있어서는 유의한 차이가 관찰되지 않았다. 결론:골수이식 후 초기시기에 주로 당 대사 및 지질대사이상이 관찰되며 이는 면역억제제 투여와 관련이 있음을 알 수 있었다. 면역억제제가 고용량 투여되는 합병증 발생 시 이들 대사이상에 관심을 기울여 대처해야 할 것이다. Background: In bone marrow transplantation(BMT), recipients are usually younger and immunosuppressants are open used in shorter period than in solid organ transplantation. Therefore, there might be a difference in glucose and lipid metabolism between BMT and solid organ transplantation. However, the serial changes of metabolic parameters following BMT have not been studied. Therefore, the aim of this study is to investigate the serial changes of blood glucose, lipids and the putative factors that are related with these changes after BMT. Methods: We have prospectively investigated 43 patients who underwent allogeneic BMT. Fasting plasma glucose(FPG), total cholesterol, triglyceride and high-density lipoprotein(HDL) were measured before BMT, and at 1, 2, 3, 4, 12 weeks and 6 months after BMT. The serial changes of these metabolic parameters according to clinical factors including type of BMT, mean daily steroid dosage, and occurrence of graft versus host disease (GVHD) were examined. Results: 1. Mean FPG level increased during 4 weeks after BMT and remained above basal value at post-transplant 6 months. Total Cholesterol level was increased during initial 4 weeks after BMT and was above basal value at post-BMT of 3 and 6 months. Triglyceride level was progressively increased during initial 4 weeks after BMT, but returned to basal value there after. HDL-cholesterol level was significantly decreased during initial 4 weeks after BMT, but returned to basal value there after. 2. Patients with FPG above 126 mg/dL and the other patients, the former received larger amounts of daily steroid and had lower HDL-cholesterol level. 3. The changes of metabolic parameters were different according to type of BMT, steroid dose, and occurrence of GVHD. Conclusion: Although there was increase of FPG, TC, TG and decrease of HDL-C during initial 4 weeks after BMT, these metabolic changes recovered slowly thereafter. Immunosuppressants are thought to be associated with these changes. Further observation will be needed for the long-term effect of BMT on metabolic changes(J Kor Diabetes Asso 24:689~698, 2000).