Tryophagus putrescentiae induces inflammatory cytokine secretions from the human cell lines THP-1 and EoL-1 cells

Jeon Sang‐Jin,Kim Eun Joo,Oh Jihyun,Yun Chi‐Young 한국곤충학회 2020 Entomological Research Vol.50 No.7

The present study aimed to identify the roles of the storage mite Tyrophagus putrescentiae (Tp) in relation to human skin diseases, such as atopic dermatitis. We examined the effects of T. putrescentiae extracts (TpE) on the secretion of inflammatory cytokines and their mRNA expression in human immune cells. Human monocytic THP-1 and eosinophilic EoL-1 cells were challenged with TpE and the inflammatory cytokines, interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) secreted in culture supernatant were analyzed using an enzyme-linked immunosorbent assay (ELISA). The mRNA levels in the cell cultures were also measured via reverse transcriptase-polymerase chain reaction (RT-PCR). In the THP-1 cells, the TpE significantly induced the secretion of IL-6, IL-8, and MCP-1 in a time- and dose-dependent manner. It also stimulated increases in the expression levels of the IL-6, IL-8, and MCP-1 mRNA. However, while the TpE induced IL-8 and MCP-1 mRNA levels in the EoL-1 cells, it did not induce IL-6 expression. Using Western blot analysis, we confirmed the activation of extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinases (MAPKs), in response to TpE challenge of the THP-1 cells. Collectively, our results show that TpE challenge elicits inflammatory response in the human monocytic THP-1 and eosinophilic EoL-1 cells, and it induces increased production of the inflammatory cytokines IL-6, IL-8, and MCP-1.

정신보건관련시설 입원(소)자의 주요 정신장애 유병률 : 전국 20개 시설의 입원(소)자를 대상으로

조성진,조맹제,서동우,함봉진,홍진표,배재남,이준영,이동우,박종익,전홍진,김성주,김용익 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.6

Objectives : One of the objectives of this study is to estimate the prevalence rates of psychiatric diagnoses in the combined populations of psychiatric hospitals, psychiatric nursing facilities and homeless asylums using the Korean version of the Composite International Diagnostic Interview (K-CIDI), which is a comprehensive and fully standardized interview schedule to assess psychiatric disorders for diagnosis. The Other objective is to compare with previously studied prevalence rates of Psy-chiatric diagnoses using the results of this study. Methods : The study subjects, aged from 181o 64 years, were randomly selected from 64,582 institutionalized population of psychiatric hospitals, Psychiatric nursing facilities and homeless asylums as of 30, June, 2001. Twelve trained interviewers administered the K-CIDI to the selected respondents. A total of 1,875 respondents (male 1,194, female 681) completed the interview. Results : The lifetime and one year prevalences of any diagnosis excluding nicotine dependence · withdrawal, anxiety disorder, eating disorder, somatoform disorder were 88.2% (male 88.1%, female 88.3%), and 65.8% (male 62.7%, female 73.7%), respectively. The lifetime and one year prevalences of schizophrenia and other psychotic disorders were 78.5% (male 75.3%, female 83.7%), and 56.8% (male 51.9%, female 64.8%), respectively. The lifetime and one year prevalences of alcohol use disorder (dependence/abuse) were 26.7% (male 37.9%, female 8.3%), and 8.7% (male 12.8%, female 2.1%), respectively. The lifetime and one year prevalences of mood disorder were 18.1% (male 13.5%, female 25.8%), and 10.4% (male 7.2%,female 16.9%), respectively. The lifetime and one year prevalences of schizophrenia and other psychotic disorders were comparable with the 2001 community survey by 1.19% to 1.32%, and 0.51% to 0.61%, respectively. The lifetime and one year prevalences of schizophrenia were also comparable by 0.16% to 0.28%, and 0.16% to 0.25%, respectively. There were no significant changes of prevalence rates when correction were applied to other psychiatric diagnosis. Conclusion : The results of this study could be used for evaluating the distribution of psychiatric diagnoses in mental health related facilities and for planning mental health policies.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

동적 후보영역과 이중 템플릿매칭을 이용한 차량 번호판 추출 및 인식

전진석,이은주 한밭대학교 정보통신전문대학원 2003 정보통신전문대학원 논문집 Vol.1 No.1

본 논문에서는 획득한 차량 영상에서, 차량 번호판의 후보영역을 동적으로 할당하여 번호판을 추출하고, 이중 템플릿 매칭을 이용하여 인식하는 방법을 제안하였다. 차량 번호판은, 이 영역이 다른 영역에 비해 영상의 흑백 변화 밀도가 높다는 것을 근거로, 번호판 후보영역을 투영하여 영상 밀도의 기준 밀도와의 차에 의하여 추출된다. 번호판 영역 추출을 실패하면, 다시 동적으로 가장 가능성이 높은 영역을 번호판 후보영역을 결정하였다. 추출된 번호판 영역에서 영역정보에 의하여 문자와 숫자영역을 분리하고,문자와 숫자의 인식은 분할된 입력 패턴과 표준패턴을 흑화소 정보에 의하여 1차 매칭하고, 유사도가 높은 표준패턴과 다시 백화소 정보에 의하여 2차 매칭하는 이중 템플릿 매칭에 의하여 하였다. In this paper, we proposed method to extract vehicle license plate by assigning dynamically candidate area, and to recognize using double template matching in captured vehicle image. We extracted vehicle license plate using density difference of candidate area and reference, because the image density of candidate area is high than other. If it failed to extract in this process, we determine dynamically candidate area. We recognized characters and numbers on plate by double template matching of input pattern and references. Then it matched black pixels first, and white next.

자궁내막에 발생한 점막 연관 림프조직 (MALT) 림프종 1예

전균호,조현진,박성균,김천복,김대연,김종혁,김용만,김영탁,목정은,남주현 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.4

Non-Hodgkin's 림프종의 약 40%는 결절외(extranodal) 림프종으로 대개 위장관에서 발생하며 이들 중 점막 연관 림프조직(mucosa-associated lymphoid tissue: MALT)에서 기원한 림프종은 MALT 림프종으로 분류된다. 위 외의 부위에서 원발성으로 발생하는 MALT 림프종은 매우 드물지만 실제 거의 모든 신체 장기에 발생하는 것으로 알려져 있는데, 가장 흔한 부위인 위 외에도 폐, 갑상선, 침샘, 눈물샘에 발생하고, 드물게 안구, 유방, 방광, 신장, 흉선 등에도 보고된 바 있다. MALT 림프종은 오랜 기간동안 원격 전이하지 않고 국소 병변으로 남아 있는 특징이 있어 치료 방향 또한 국소 병변의 치료에 집중되며, 예후도 림프절 기원의 림프종에 비해 양호한 것으로 알려져 있다. 본 저자 등은 자궁 내막에 원발성으로 발생한 MALT 림프종 1예를 경험하였기에 문헌 고찰과 함께 이를 보고하고자 한다. Mucosa associated lymphoid tissue (MALT) lymphoma can rarely develop primarily in extranodal sites other than stomach which is the most common site for it. Other rare primary sites are small intestine, colorectum, esophagus, lung, thyroid, salivary gland, lacrymal gland, breast and skin. MALT lymphoma represents a distinct clinicopathologic features: it is usually localized to their original site for a long time and shows much more favorable prognosis than lymphoma at other site, but some MALT lymphoma can arise simultaneously or successively in different organ or give rise to another MALT lymphoma of other organ and can be multifocally disseminated or recurred. We report a very rare case of high grade B-cell lymphoma of mucosa associated lymphoid tissue (MALT) of the uterine endometrium, which was confirmed by immunohistochemical assay.

국내에서 유행하는 HIV의 전파 경로에 따른 Subtype 분포

이주실,남정구,김성순,강춘,최병선,김옥진,박미선,성봉모,서순덕,전수경,변승옥,신영오,조해월 대한감염학회 2001 감염 Vol.33 No.5

Background : Previous data have been reported that subtype B is prevalent in South Korea, but neither the extent nor the proportion of subtypes could be evaluated. This study was designed to analyze the distribution of HIV-1 subtypes, temporal instructions and transmission dynamics between epidemiological groups. Methods : 1,280 Koreans had been diagnosed as HIV seropositive during the period 1985 to 2000. Among them, 134 individuals were selected for this molecular epidemiological study. 134 DNAs were isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and was sequenced. Results : HIV-1 isolates from thirty-seven homosexuals were all subtype B (100%). On the other hand, 66 isolates from 94 heterosexuals were subtype B (70%) and 28 were non B subtypes (30% : 13 A, 4 C, 2 D, 8 E , 1 G). Only subtype B strains were isolated from 73 males who were infected with HIV inside Korea while 16 B and 20 non B subtype strains were isolated from 36 males who were HIV infected outside of Korea. However, B and non B strains were isolated half and half from females who were infected inside Korea except one. Conclusion : The HIV-1 subtype B strains are prevalent in Korea from the early HIV infection until present in both homo and heterosexuals. Non B strains have been transmitted from men who were infected outside Korea to their spouses and casual partners. So, we need further study to monitor subtype B and non B HIV transmission in epidemiological groups of Korea, (Korean J Infect Dis 33:311∼318, 2001)

의미 치매 1예 : 신경심리 및 뇌영상 검사의 연속적인 추적 관찰 소견 Serial Follow-up Study of Neuropsychological and Neuroimaging Findings

박경원,김욱주,전진민,강도영,김재우 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.1

Semantic dementia (SD) is a very rare, distinct form of dementia which is characterized by impairment of naming and comprehension, prosopagnosia, visual agnosia for objects and relative preservation of other aspects of cognition, such as episodic memory, nonverbal problem-solving and visuospatial functions. A 68-year-old man visited our clinic for progressive speech disturbance since 1997. His main speech problems were word finding difficulty and loss of word meaning for objects. Language assessment demonstrated profound semantic memory loss for words with preserved fluency, corresponding to anomic aphasia. There were marked impairments in verbal and visual memory functions and word fluency tasks, but no grammatical or phonologic errors in speech. Also were remarkable anosognosia and object visual agnosia with preserved visuospatial function and calculation in neuropsychological tests. Serial follow-up study of language test showed more deterioration in comprehension and repetition, consistent with Wernicke's aphasia. Initial brain MR imaging and brain perfusion SPECT showed prominent focal atrophy in the left temporal lobe, and marked hypoperfusion in the left temporal and both frontal areas. Follow-up brain MR imaging and perfusion SPECT revealed more atrophy in both temporal lobes and showed more extensive hypoperfusion areas compared to the previous study.

Bipolar Host Materials for Green Triplet Emitter in Organic Light-emitting Diodes

Jeon, Ji Young,Park, Tae Jin,Jeon, Woo Sik,Park, Jung Joo,Jang, Jin,Kwon, Jang Hyuk,Lee, Jae Yeol The Chemical Society of Japan 2007 Chemistry letters Vol.36 No.9

<P>We have developed novel bipolar host materials for high efficiency green phosphorescent OLEDs (PHOLEDs). Phenyl moieties were inserted in a 9,9′-(biphenyl-4,4′-diyl)dicarbazole (CBP) compound to provide much easier electron injection and to increase electron mobility. The efficiency increase and voltage reduction by this modification were observed in green PHOLEDs. At a given constant luminance of 1000 cd/m<SUP>2</SUP>, the power efficiency was enhanced about twenty percent in the general green PHOLED devices.</P>

Case Reports : A Case of Bronchiolitis Obliterans Organizing Pneumonia Associated with Adenovirus

Jin Seok Jeon,Hyeon A Yi,Shin Young Ki,Seong Whan Jeong,Soo Taek Uh,So Young Jin,Jai Soung Park,Deuk Lin Choi,Chang Hee Kang,Joo Sung Kim,Choon Sik Park 대한내과학회 1997 The Korean Journal of Internal Medicine Vol.12 No.1

Inhibitory effect of carvacrol on melanin synthesis via suppression of tyrosinase expression

Jeon, Nam-Joo,Kim, Yon-Suk,Kim, Eun-Kyung,Dong, Xin,Lee, Jae-Woong,Park, Jin-Su,Shin, Woen-Bin,Moon, Sang-Ho,Jeon, Byong-Tae,Park, Pyo-Jam Elsevier 2018 Journal of Functional Foods Vol.45 No.-

<P><B>Abstract</B></P> <P>Carvacrol (2-methyl-5-(1-methylethyl)-phenol) compound derived from oils of Thymus vulgaris is a natural member of monoterpene phenol. It has been shown that carvacrol exhibit anti-microbial, anti-inflammatory, anti-oxidant activities. However, no studies have reported its anti-melanogenesis effect. Therefore, the objective of this study was to investigate the anti-melanogenic potential of carvacrol. Results of this study confirmed that carvacrol could regulate protein expression levels of microphthalmia-associated transcription factor [MITF, a protein closely related to transcription of cAMP response element-binding protein (CREB)] and various enzymes involved in melanin synthesis. Moreover, this study provided evidence that carvacrol could regulate the degradation of MITF protein by extracellularly responsive kinases (ERK) phosphorylation. Carvacrol strongly inhibited the synthesis of CREB protein, tyrosinase-related protein 1 (TRP-1), and tyrosinase known to be important enzymes involved in melanogenesis. These results indicate that carvacrol can inhibit melanin synthase by decreasing enzyme expression levels important for melanin synthesis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Carvacrol inhibited melanin synthesis in a dose-dependent manner. </LI> <LI> Carvacrol down-regulated both the protein and mRNA levels of tyrosinase. </LI> <LI> Carvacrol decreased microphthalmia-associated transcription factor (MITF). </LI> <LI> Carvacrol decreased MITF protein via the phosphorylated ERK signaling. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>