1995년도 무균성 뇌막염 환아에서 원인바이러스 동정

강완복,윤재득,김동수,김문보,김기순,서순덕 대한감염학회 1996 감염 Vol.28 No.4

목 적 : 뇌막염은 뇌막의 염증성 반응으로 여러 가지 병원균에 의해서 올 수 있다. 특히 소아에서는 무균성 뇌막염이 많은 것으로 알려져 있고 이중 대다수가 바이러스에 의한 것이다. 특히 장바이러스가 무균성 뇌막염의 약 85 % 를 차지하고 있다. 1993년도 우리 나라에서는 echovirus 9, 30 에 의한 뇌막염의 전국적인 대유행이 있었고 1994년도에는 echovirus 3 에 의하여 뇌막염이 초래되었다. 이러한 뇌막염의 원인 바이러스는 매년 달라질 수 있으므로 1995년도 봄철에 뇌막염으로 입원하는 환자들의 원인 바이러스를 동정하여 보는 바이다. 방 법 : 1995년도 5월과 6월에 걸쳐 연세대학교 의과대학 세브란스병원 소아과에 입원하여 무균성 뇌막염으로 진단된 환아 19 례 를 대상으로 임상적 관찰 및 바이러스동정을 시행하였다. 가검물은 환아의 뇌척수액과 대변을 동시에 채취하여 배양된 RD 세포와 HEp-2 세포에 접종하여 세포병변을 관찰한 후 enterovirus serum pool 에 의한 중화시험을 하였다. Piore 와 Nicoletta 의 방법에 의하여 세포배양액에서 RNA 를 추출하고 PCR 에 의하여 oligonucleotide 를 합성하였다. 그 후 noncoding RNA PCR 에 의하여 DNA 를 합성하고 전기영동을 거쳐 확인하였다. 결 과 : 1) 임상양상 및 경과는 전형적인 무균성 뇌막염의 양상을 보였다. 2) 총 19 례 중에서 8 례 에서 enterovirus 가 자랐으며, 이 중 5례 에서는 coxsackie B3 가 동정되었으며 3 례 에서는 echovirus 7 이 1 례 에서는 echovirus 3 이 동정되었다. 이중 1 례 에서는 coxsackie B3 와 echovirus7 이 동시에 동정되었다. 3) PCR product도 전형적인 장 바이러스에 합당한 소견을 보였다. 결 론 : 1995년도 봄철에 연세대학교 의과대학 세브란스병원 소아과에 무균성 뇌막염으로 입원한 환자의 원인바이러스는 coxsackie B3 와 echovirus 7 에 의한 감염이었다. Background : Aseptic meningitis is a common illness during childhood. Although the etiologic agent is not always identified, viruses are the usual responsible agents. Enterovirus especially causes approximately 85% of all cases of aseptic meningtis. In 1993, there was a nationwide epidemic of aseptic meningitis by echovirus 9 and 30. And we reported that the cause of aseptic meningitis in 1994 was echovirus 3. This study was done to detect the causative virus of aseptic meningitis in 1995. Methods : To isolate the causative viruses, stool and cerebrospinal fluid specimens from the patients with aseptic meningitis, who were admitted to Severance Hospital in 1995, were collected. Cultured RD cells and HEp-2 cells were inoculated with specimens to see the cytopathic effects. Neutralizing antibody tests using enterovirus serum pool were done on the specimens with the cytopathic effects. RNA was isolated from the cultured supernatants of the infected cells by Piore and Nicoletta method. Oligonucleotide was synthesized by PCR, which was run on polyacrylamide gel after purification with HPLC. After running the DNA produced by using Geneamp RNA PCR Kit, electrophoresis was done. Results : Enterovirus was isolated from 8 out of 19 patients. Among these eight, Coxsackie B3 was isolated in 5 patients. Echovirus 7 was isolated in 3 patients. In one of these patients, Coxsackie B3 and echovirus 7 was isolated simultaneously. Echovirus 20 was isolated in one patient. PCR product from these viruses showed a 154 bp band on gel electrophoresis, which was the same PCR product from echovirus 3 supplied from ATCC. Conclusion : The causative viruses of aseptic meningitis in patients who were admitted to Severance Hospital during the spring season of 1995 were Coxsackie B3 and echovirus 7.

HIV 항체 효소면역시험에 대한 정도평가 분석

김성순,서순덕,김옥진,이민우,이주실,김현숙,김영란,박광일,황병갑,이용래,조연정,황유성 대한임상검사정도관리협회 1992 臨床檢査와 精度管理 Vol.22 No.2

HIV 항체 효소면역시험에 대한 정도평가 분석

김성순,서순덕,김옥진,이민우,김현숙,김영란,박광일,황병갑,이용래,조연정,황유성,이주실 국립보건연구원 2000 국립보건원보 Vol.37 No.-

1994년도 무균성 뇌막염 환아에서 원인바리러스 동정

김종현,김문보,윤재득,김기순,서순덕,박재현,김동수 대한감염학회 1995 감염 Vol.27 No.1

목 적 : 뇌막염은 뇌막의 염증성 반응으로 여러가지 병원균에 의해서 올 수 있다. 특히 소아에서는 무균성 뇌막염이 많은 것으로 알려져 있고 이중 대다수가 바이러스에 의한 것이다. 특히 장바이러스가 무균성 뇌막염의 약 85%를 차지하고 있다. 1993년도 우리나라에서는 echovirus 9, 30에 의한 뇌막염의 전국적인 대유행이 있었다. 이러한 원인 바이러스는 매년 달라질 수 있으므로 1994년도 봄철에 뇌막염으로 입원하는 환자들의 원인 바이러스를 동정하여 보고하는 바이다. 방 법 : 1994년도 5월과 6월에 걸쳐 연세대학교 의과대학 세브란스 소아과에 입원하여 무균성 뇌막염으로 진단된 환아 11예를 대상으로 임상적 관찰 및 바이러스 동정을 시행하였다. 가검물은 환아의 뇌척수액과 대변을 동시에 채취하여 배양된 RD세포와 HEP-2 세포에 접종하여 세포병변을 관찰한 후 enterovirus serum pool 에 의한 중화 시험을 하였다. Piore와 Nicoletta의 방법에 의하여 세포배양액에서 RNA를 추출하고 PCR에 의하여 oligonucleotide를 합성하였다. 그 후 noncoding RNA PCR에 의하여 DNA를 합성하고 전기영동을 거쳐 확인하였다. 결 과 : 1) 임상양상 및 경과는 전형적인 무균성뇌막염의 양상을 보였다. 2) 총 11예 중 4예에서 echovirus가 자랐으며, 1예에서 뇌척수액에서만 배양된 것을 제외하고는 3예에서 뇌척수액과 대변에서 모두 echovirus 3가 배양되었다. 1예에서는 뇌척수액에서 echovirus 3이외에 coxsackie B virus가 검출되었다. 3) PCR product도 전형적인 장바이러스에 합당한 소견을 보였다. 결 론 : 1994년도 봄철에 연세대학교 의과대학 세브란스병원 소아과에 무균성뇌막염으로 입원한 환자의 원인 바이러스는 echovirus 3에 의한 감염이었다. Background : Aseptic meningitis, an acute inflammation of the meninges, is a common illness during childhood. Although the etiologic agent is not always identified, viruses are the usual responsible agents. Enterovirus especially causes approximately 85% of all cases of aseptic meningitis. In 1993, there was a nationwide epidemic of aseptic meningitis by echovirus 9 and 30. This study was done to detect the causative virus of aseptic meningitis in 1994. Methods : To isolate the causative viruses, stool and cerebrospinal fluid specimens from the patients with aseptic meningitis, who were admitted to Severance Hospital in 1994, were collected. Cultured RD cell and HEP-2 cell were inoculated with specimens to see the cytopathic effects. Neutralizing antibody tests using enterovirus serum pool were done on the specimens with the cytopathic effects. RNA was isolated from the cultured supernatants of the infected cells by Piore and Nicoletta method. Oligonucleotide was synthesized by PCR, which was run on polyacrylamide gel after purification with HPLC. After running the DNA produced by using Geneamp RNA PCR Kit, electrophoresis was done. Results : Echovirus was isolated from 4 out of 11 patients. Among these four, echovirus 3 was isolated from both CSF and stool in three patients. In one of these three, Coxsackie B virus was isolated as well from CSF. In the remaining one patient, echovirus 3 was present in CSF only. PCR product from this virus showed a 114 bp band on gel electrophoresis, which was the same PCR product from enterovirus isolated from stool specimens of the patients with aseptic meningitis in 1993. Conclusion : The causative virus of aseptic meningitis in patients who were admitted to Severance Hospital during the spring season of 1994 was Echovirus 3.

1993년 한국에서 발생한 Aseptic meningitis 환자로부터 장내바이러스 RNA 검출

김문보,김기순,김창민,서순덕,윤재득,신학균 대한바이러스학회 1994 Journal of Bacteriology and Virology Vol.24 No.1

Aseptic meningitis is known that the sign of neurological diseases are present except paralysis even though the main sign and symptoms are fever and headache with nuchal rigidity of which is stiffness of the neck muscles due to meningeal irritation but the damage is minor. Outbreakes are very common in young aged group during the warm season through the infection is spread mainly by the fecal-oral route from virus excretors to contacts and virus in pharyngeal secretion is also source of infection. Since sporadic clinical cases of aseptic meningitis has reported in 1990, one of epidemic during the month of May, 1993 due to let enterovirus teams in NIH, Korea carried out seroepidemiological investigation against cases of aseptic meningitis which is a first time in Korea. Stool and CSF specimens from 3S patients with aseptic meningitis were tested by neutralization test and PCR derived from the highly conserved 5-noncoding enterovirus' genomic region. We found a higher infection rate in the 1-9 years old group(68.5 %) and in the male sex(68.5 / ). ECHO type 9 and 30 were isolated in fecal specimens and coxsackie type B was isolated in CSF. The PCR assay yielded positive results in 7 cases where enterovirus diagnosis were obtained by serological test.

C형 간염 바이러스의 검출 및 유병률 조사 : 우리나라 C형간염의 의학적 특성

윤재득,김문보,김기순,서순덕,안광수,신학균,정상인 국립보건연구원 1994 국립보건원보 Vol.31 No.1

한국인에서 E형 간염 바이러스의 항체 유병률에 관한 조사

신학균,윤재득,정연호,김문보,서순덕,김정서 대한바이러스학회 1992 Journal of Bacteriology and Virology Vol.22 No.2

Serosurvey of hepatitis E virus (HEV) infection, known as enterically transmitted non-A, non-B hepatitis (ET-NANBH), was done in Korea between May through August, 1991 using anti HEV EIA method developed by Genelabs Inc. in 1990. The coated polypeptide antigens were expressed in Baculovirus vector from the specific HEV cDNA clones derived from two patients infected with HEV, one from Burma (antigen A) and the other from Mexico (antigen B). The 1,079 examinees was arbitrarily grouped into risk (406) and non-risk (673) group according to the serologic markers of hepatitis B virus (HBsAg, HBsAb, and HBcAb), anti-hepatitis C virus (HCV), and alanine transarninase (ALT) value but not to the risk of HEV. The overall seroprevalences of HEV in non-risk and risk group were 10.4 %, and 8.9%, respectively. Among the non-risk subgroups, the prevalence of anti-HEV was about 10 % regard- less of the result of HBsAb or HBcAb. There was no sexual and regional difference. The peak prevalence was shown at the age between 30 and 39. In the risk groups, the highest prevalence was 15% shown in the anti-HCV positive group and the lowest was the 5.9% of HBsAg positive group. There was a little higher prevalence rate in male group but there was no significant statistical difference between male and female. Anti-HEV posit,ive rates of normal group were relatively higher than the expected risk group and these results strongly suggested the possibility of existence of HEV infection in Korea, particularly in rural areas where the tap water supply facilities are not enough.

HIV 신규 감염자의 초기 CD4+ T 세포 수 변화분석 (1990-2000.6)

김창오,신행섭,최병선,이준영,서순덕,김기순,오운성,함일우,권준욱,이주실 대한감염학회 2003 Infection and Chemotherapy Vol.35 No.6

우리나라의 C형간염 바이러스 항체 분포

신학균,윤재득,유재창,김문보,김기순,서순덕 대한바이러스학회 1993 Journal of Bacteriology and Virology Vol.23 No.2

Enzyme immuno assay (EIA) which utilizes a solid phase coated with a recombinant antigen derived from the hepatitis C virus (HCV) genome was evaluated for efficacy in the detection of anti-HCV. Using the antigen expressed on E. coli from a cDNA clone encoding structural region and non-structural region of HCV genome, the prevalence of HCV antibody on 2858 peoples in Korea was investigated. Prevalence of antibodies to HCV is 1.2% in townsmen, 0.7% in farm village residents and 2.7% in seashore residents considered to be at low risk for exposure to HCV. These limited data also indicate that the prevalence of antibodies to HCV is 31.0% in commercial plasma donors, 10.6% in HIV(Human Immunodefecience Virus) antibody positive group and 4.8% in prostitutors considered to be at high risk for exposure to HCV. And 51(45.9%) sera were positive for hepatitis B core antibody, 3(2.7% ) sera were positive for hepatitis B surface antigen and 27(24.3%) sera were elevated ALT(alanine aminotransferase) concentration among 111 sera which were confirmed anti-HCV positive. We confirmed 10 specimens have had RNA of hepatitis C virus among 25 anti-HCV positive specimens by polymerase chain reaction(PCR) and southern blot hybridization, simultaneousely, comparative test was carried out among 8 commercial anti-HCV kits in Korea, and 6 kits had shown specific homology more than 95%. These seroepidemiological data suggest that HCV plays an important role as an etiological agent in Korea.

HIV env 유전자의 V1-V5 부위 분석에 의한 HIV 분리주의 subtype 및 변이 분석

김성순,강춘,박근용,남정구,최병선,기미경,서순덕,이성래,이주실 국립보건연구원 1999 국립보건원보 Vol.36 No.-