Tryophagus putrescentiae induces inflammatory cytokine secretions from the human cell lines THP-1 and EoL-1 cells

Jeon Sang‐Jin,Kim Eun Joo,Oh Jihyun,Yun Chi‐Young 한국곤충학회 2020 Entomological Research Vol.50 No.7

The present study aimed to identify the roles of the storage mite Tyrophagus putrescentiae (Tp) in relation to human skin diseases, such as atopic dermatitis. We examined the effects of T. putrescentiae extracts (TpE) on the secretion of inflammatory cytokines and their mRNA expression in human immune cells. Human monocytic THP-1 and eosinophilic EoL-1 cells were challenged with TpE and the inflammatory cytokines, interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) secreted in culture supernatant were analyzed using an enzyme-linked immunosorbent assay (ELISA). The mRNA levels in the cell cultures were also measured via reverse transcriptase-polymerase chain reaction (RT-PCR). In the THP-1 cells, the TpE significantly induced the secretion of IL-6, IL-8, and MCP-1 in a time- and dose-dependent manner. It also stimulated increases in the expression levels of the IL-6, IL-8, and MCP-1 mRNA. However, while the TpE induced IL-8 and MCP-1 mRNA levels in the EoL-1 cells, it did not induce IL-6 expression. Using Western blot analysis, we confirmed the activation of extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinases (MAPKs), in response to TpE challenge of the THP-1 cells. Collectively, our results show that TpE challenge elicits inflammatory response in the human monocytic THP-1 and eosinophilic EoL-1 cells, and it induces increased production of the inflammatory cytokines IL-6, IL-8, and MCP-1.

Analysis of Short-Term Prestress Losses in Post-tensioned Structures Using Smart Strands

Sang?Hyun Kim,Sung Yong Park,Sung Tae Kim,Se?Jin Jeon 한국콘크리트학회 2022 International Journal of Concrete Structures and M Vol.16 No.1

소음인(少陰人) 승양익기부자탕(升陽益氣附子湯)과 관계부자리중탕(官桂附子理中湯)이 양허증(陽虛證)에 미치는 영향(影響)에 관(關)한 실험적(實驗的) 연구(硏究)

전진상,Jeon, Jin Sang 사상체질의학회 1989 사상체질의학회지 Vol.1 No.1

In order to investigate experimentally the effects of Sung-yangikgibujat'ang (SIT) and Kwangyebujalijungt'ang (KBT) on Yang-insufficient syndrome (陽虛證) induced by Hydrocortisone acetate (H.A.) in experimental animals (Mice and Rat), the author experimented various activities. Delayed type hypersensitivity (DTH), Rosette forming cells (RFC), hemagglutinin (HA) titers, Hemolysin (HL) titers, Body weight, Whole blood viscosity, Plasma Viscosity, Hematocrit, RBC, Albumin, Total protein, Triglyceride, cholesterol and Glucose were measured. The results summerized as follows; 1. In DTH and RFC all the experimental groups were increased significantly in comparison to the H.A.-treated group. 2. In HA titers SIT_treated group were increased significantly and KBT-treated group showed increasing tendancy, but showed no significance. 3. In HL titers all the experimental groups showed increasing tendancy, but showed no significance. 4. In body weight all the experimental groups showed increasing tendancy, but showed no significance. 5. In whole blood viscosity all the experimental groups were decreased significantly in comparison to the H.A.-treated group. 5. In whole blood viscosity all the experimental groups were decreased significantly in comparison to the H.A.-treated group. 6. In plasma viscosity KBT-treated group were decreased significantly and SIT-treated group showed decreasing tendancy, but showed no significance. 7. In Hematocrit SIT-treated group were decreased significantly and KBT-treated group showed decreasing tendancy, but showed no significance. 8. In RBC, albumin and cholesterol all the experimental groups were decreased significantly in comparison to the H.A.-treated group. 9. In total protein and triglyceride KBT-treated group were decreased significantly and SIT-treated group showed decreasing tendancy, but showed no significance. 10. In Glucose SIT-treated group were decreased significantly and KBT-treated group showed decreasing tendancy, but showed no significance. From above findings, it has been demonstrated that Sungyangikgibujat'ang and Kwangyebujalijungt'ang seem to produce the effectiveness on the recovery from depression of the cell-mediated immune response, blood circulation and energy metabolic rate, induced by Hydrocortisone acetate, and in the humoral immune response Sungyangikgibujat'ang have the effectiveness on the recovery, and in cellular component of blood Sungyangikgibujat'ang was more effective than Kwangyebujalijungt' ang, and in plasma of blood Kwangyebujalijungt'ang was more effective than Sungyangikgibujat'ang. Therefore it is suggested that Sungyangikgibujat'ang and Kwangyebujalijungt'ang have the effectiveness on the recovery from Yang-insufficient syndrome more or less.

개발사업 환경성평가에서의 토양 탄소저장량 산정방법 비교 연구

황상일 ( Sang Il Hwang ),박선환 ( Sun Hwan Park ),전영봉 ( Young Bong Jeon ),황정배 ( Joung Bae Hwang ),강선홍 ( Seon Hong Kang ),김진홍 ( Jin Hong Kim ) 한국환경영향평가학회 2014 환경영향평가 Vol.23 No.6

The objective of this study was to propose the best methodology for estimating soil organic carbon stocks during environmental assessment for development projects. We compared three methodologies which were developed by Korea Environment Corporation(2010), Korea Forest Research Institute (2006), and Jin-Hyun Jung (1998). We found that the methodology developed by Jin-Hyun Jung (1998) shows the worst performance and the methodology of Korea Forest Research Institute (2006) does not reflect a variety of soil types and land use characteristics shown in development project plans. Therefore, we propose that the methodology developed by Korea Environment Corporation (2010) is the most reasonable one because it is internationally accepted and used for local governments to make the inventory of greenhouse gases as well as to set up its reduction strategy.

Interaction of <i>Veratrum nigrum</i> with <i>Panax ginseng</i> against Obesity: A Sang-ban Relationship

Park, Jinbong,Jeon, Yong-Deok,Kim, Hye-Lin,Lim, Hara,Jung, Yunu,Youn, Dong-Hyun,Jeong, Mi-Young,Kim, Hyun-Ju,Kim, Sung-Hoon,Kim, Su-Jin,Hong, Seung-Heon,Um, Jae-Young Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Obesity has become a major health threat in developed countries. However, current medications for obesity are limited because of their adverse effects. Interest in natural products for the treatment of obesity is thus rapidly growing. Korean Medicine (KM) is characterized by the wide use of herbal formulas. However, the combination rule of herbal formulas in KM lacks experimental evidence. According to <I>Shennong's Classic of Materia Medica</I>, the earliest book of herbal medicine, <I>Veratrum nigrum</I> (VN) has antagonistic features against <I>Panax ginseng</I> (PG), and the PG-VN pair is strictly forbidden. In this study, we have shown the effects of PG, VN, and their combination on obesity in high-fat (HF) diet-induced obese mice and in 3T3-L1 cells. PG, VN, and PG-VN combination significantly reduced weight gain and the fat pad weight in HF diet-induced obese mice. They also significantly decreased lipid accumulation and the expressions of two major adipogenesis factors, PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I>, in 3T3-L1 cells. In addition, the PG-VN combination had synergistic effects compared with the mixture of extracts of PG and VN on inhibition of PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I> expressions at lower doses. These results indicate a new potential anti-obese pharmacotherapy and also provide scientific evidence supporting the usage of herbal combinations instead of mixtures in KM.</P>

고조리서와 종가의 무김치 비교 연구

이현진,이상원,전형주,정혜정,Lee, Hyun-Jin,Lee, Sang-won,Jeon, Hyeong-ju,Chung, Hea-jung 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.5

The purpose of this research aims to reveal how radish kimchi (Jong-ga) differs in Jong-ga recipes and old cookbooks. To accomplish this, old cookbooks ("Soowoonjabbang", "Jusiksiui", "Eumsikbangmunnira", "Siuejunsuh", "Banchandeungsok", and "Buinpilji") were reviewed and 8 Jong-ga recipes (Seogye Park Se-dang from the Bannam Park clan Jong-ga, Myungsukgong from the Changnyeong Jo clan Jong-ga, Nampa Park Jae-gyu from the Milyang Park clan Jong-ga, Geunggudang Kim Joong-jeung from the Gwangsan Kim clan Jong-ga, Dongchundang Song Jun-gil from the Eunjin Song clan Jong-ga, Myeongjae Yun Jung from the Papyung Yun clan Jong-ga, Daeseunggong Ryu Cha-dal from the Munhwa Ryu clan Jong-ga, Inmukjae Son Sung-jeung from the Milseong Son clan Jong-ga) from five areas were reviewed. We classified the radish kimchi into five categories, radish kimchi, Dongchimi, kkakdugi, Seokbakji and Nabak kimchi and other kimchi. According to old cookbooks, most kimchi was made with radish, cabbage, cucumber, pear, yuju, fish meat, and salt. Modern Jong-ga is made of seasoned radish, sticky rice paste, seafood, sugar, powdered pepper, fish sauce and salt. This study helps to understand notable clans' cultures via their recipes for kimchi.

A comparative study on determinants of housing mortgage prepayment of individual borrowers

( Sang Won Lee ),( Su Bok Ryu ),( Tae Young Kim ),( Jin Q. Jeon ) 한국파생상품학회 2022 선물연구 Vol.30 No.4

This paper examines how the macroeconomic environment affects the determinants of prepayment of mortgage loans from October 2004 to February 2020. For more accurate analysis, the authors define the timing of prepayment not only before the loan maturity but also at the time when 50% or more of the loan principal is repaid. The results show that, during the global financial crisis as well as the recent period of low interest rates, macroeconomic variables such as interest rate spreads and housing prices have a different effect compared to the normal situation. Also, significant explanatory variables, such as debt to income (DTI) ratio, loan amount ratio and poor credit score, have different effects depending on the macroenvironment. On the other hand, in all periods, the possibility of prepayment increases as comprehensive loan to value (CLTV) increases, and the younger the age, the shorter the loan maturity. The results suggest that, in the case of ultralong (40 years) mortgage loans recently introduced to support young people purchasing houses, the prepayment risk can be, at least partially, migrated by offsetting the increase in prepayment by young people and the decrease in prepayment due to long loan maturity. In addition, this study confirms that the accelerated time failure model compared to the logit model and COX proportional risk model has the potential to be more appropriate as a prepayment model for individual borrower analysis in terms of the explanatory power.

Genomic Alterations of Anaplastic Thyroid Carcinoma Detected by Targeted Massive Parallel Sequencing in a <i>BRAF^V600E</i> Mutation-Prevalent Area

Jeon, Min Ji,Chun, Sung-Min,Kim, Deokhoon,Kwon, Hyemi,Jang, Eun Kyung,Kim, Tae Yong,Kim, Won Bae,Shong, Young Kee,Jang, Se Jin,Song, Dong Eun,Kim, Won Gu Mary Ann Liebert 2016 Thyroid Vol.26 No.5

Numerical Prediction of Maximum Load-Carrying Capacity of Cracked Alloy 690TT Steam Generator Tubes

Jeon, Jun-Young,Kim, Yun-Jae,Kim, Jin-Weon,Lee, Kuk-Hee,Kim, Jong-Sung AMERICAN SOCIETY MECHANICAL ENGINEERS 2016 Journal of Pressure Vessel Technology Vol.138 No.4

<P>This paper presents a finite element (FE) simulation technique to predict maximum load-carrying capacity of cracked steam generator tubes and its application to Alloy 690TT tubes. The simulation method is based on a simplified version of the stress modified fracture strain model. The damage model is determined from tensile test and one cracked tube test data. Predicted maximum pressures are compared with 23 test data of axial through-wall and surface cracked Alloy 690TT steam generator tubes. Comparison with experimental data shows good agreement.</P>

Effect of Fluorescent Light Treatment during Imbibition and Culture on Growth of Soybean Sprout

Jin Ho Kang,A-Jung Park,Byung Sam Jeon,Soo Young Yoon,Sang Woo Lee 韓國作物學會 2003 Korean journal of crop science Vol.48 No.4

The lateral root formation in soybean sprout culture declines its quality. This study was done to measure the effect of fluorescent light treatment during 24 hour imbibition and 6-day culture on seed germination and growth of soybean sprout. After 6 day culture, the sprouts were sorted as normal (>4cm), abnormal (<4cm) and non-germination by their hypocotyl lengths, and lateral roots, fresh and dry weights were measured. Lateral roots were less formed in the fluorescent light treatment lasted during the whole period of the imbibition than in the treatment for 50 minutes a day during the culture. The fluorescent light treatment during the imbibition mainly affected the germination and growth compared to the treatment done during the culture. Compared to the dark imbibition, the light treatment during the imbibition resulted in more normal sprouts, thicker diameters of hypocotyl and hook, and more fresh weights in cotyledon, hypocotyl, whole sprout, and economic yield. However, these results were reverse in lengths of hypocotyl and root, and fresh and dry weights of roots. It is concluded that the fluorescent lamp mainly irradiating red and blue lights can be used for the sprout production as an alternative light replacing blue and red lights treated during the imbibition because it blocked the lateral root appearance and stimulated growth of the sprout.