급격한 온도 변화에 순응한 모래무지(Peudogobio esocinus) 젖산탈수소효소 활성과 동위효소의 변화

김재범,김선균,염정주 청주대학교 산업과학연구소 2003 産業科學硏究 Vol.21 No.1

Changes of activities and isozymes of lactate dehydrogenase(EC 1.1.1.27, LDH) in Pseudogobio esocinus acclimated to acute change of temperature were studied. The LDH activities of heart, liver, eye, and brain acclimated to 10℃ were detected higher level than those acclimated to 20℃. And the LDH activity of skeletal muscle showed higher level when acclimated to 20℃. And acclimation temperature had no significant effect on the LDH activity of the heart. When the temperature was acutely changed, the variation of the LDH activity had maximum state within 2 hours. The activity of subunit which has higher activity among subunit A and B was decreased and that of the subunit having lower activity was increased. The metabolism of liver tissue was regulated by the increase of C₄ isozyme activity and the decrease of A₄ isozyme. LDH was played role as lactate oxidase in skeletal muscle, heart, and liver, and as pyruvate reductase in eye and brain tissue. As a result, the change of LDH activity and isozyme to acute increase of temperature might be the acclimation process to the control of metabolism with surroundings.

비강 및 비인두에 발생한 도립유두종과 편평상피암종의 p53단백 및 세포증식능에 관한 연구

한주호,윤기중,이재규,신대균,박근호,조향정,문형배 圓光大學校 醫科學硏究所 1996 圓光醫科學 Vol.12 No.2

The inverted papilloma and squamous cell carcinoma are common neoplasia in the sinonasal cavity and nasopharynx, but the incidence of these tumors are very low and the study on the oncogenesis or biological activity of the tumor cells are not well known. This study was designed to evaluate the oncogenic roles of the p53 gene and the proliferative activity of the tumor cells in the inflammatory polyp, inverted papilloma and squamous cell carcinoma. The experiment was carried by the immunohistochemical stains about the p53 protein, PCNA and Ki-67, histochemical stain about the AgNORs. and flow cytometric analysis about the DNA ploidy using the formalin fixed paraffin embedded tissues. The frequency of the expression of p53 protein was 0%(0/16 cases) in the inflammatory polyps, 18.8% (3/16 cases) in the inverted papillomas, and 87.5%(14/16 cases) in the squamous cell carcinomas. The labelling index(%) of the PCNA and Ki-67 was 5.3% and 3.3% in the inflammatoy polyps, 29.6% and 25.2% in the inverted papillomas, and 51.9% and 36.8% in the squamous cell carcinoma. The expression of the PCNA and Ki-67 was distributed in the periphery of the tumor islands of the inverted papilloma and was distributed in the both of center and periphery of the tumor islands of the squamous cell carcinoma. The number of nuclear AgNORs was increased in the order of inflammatory polyps (0.96), inverted papillomas(1.34) and squamous cell carcinoma(2.09). The frequency of the DNA aneuploidy was 0%(0/16 cases) in the inflammatory polyps, 18.8%(3/16 cases) in the inverted papillomas. and 12.5%(2/16 cases) in the squamous cell carcinomas. Above results indicates that the changes of the p53 gene and proliferative activity of the tumor cells are involved on the oncogenesis and the biological activity of the inverted papilloma or squamous cell carcinoma in the nasopharynx and sinonasa cavity.

공여자의 종류에 따른 간이식 후 감염성 합병증의 비교 : Comparison between Orthotopic and Living Donor Transplantation

기현균,손준성,오원섭,백경란,이남용,김성주,조재원,이석구,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 최근 간이식 수요가 증가함에 따라 이에 대한 하나의 대안으로 생체 부분 간이식(LDLT) 시행례가 증가하고 있다. 이에 뇌사자 사체 간이식(OLT)과의 감염 양상을 비교하기 위하여 본 연구를 시행하였다. 방법 : 1996년 5월부터 2003년 8월까지 삼성서울병원에서 간이식을 시행 받은 284명의 환자들을 대상으로 후향적으로 분석하였다. 결과 : 188명은 LDLT를, 96명은 OLT를 시행 받았다. 간이식 후 감염증은 총 191명에서 발생하였고(67.0%) OLT 환자군과 LDLT 환자군 사이에 감염증의 발생 빈도와 원인 병원체의 빈도는 유의한 차이를 보이지 않았다. 병원체 별로는 Enterococcus 균정(16.2%) S. aureus(15.1%), coagulase negative staphylococci (13.4%)의 순서였다. 감염증의 종류는 복강 내 감염이 총 83례로 가장 많았는데, LDLT에서 OLT에 비하여 유의하게 높게 발생하였다(34.0% vs 19.8%, P=0.012). 요로 감염증(11.5% vs 4.3%, P=0.022)과 침습성 아스페르길루스증(7.3% vs 1.6%, P=0.034)은 OLT을 시행한 경우에 많았다. 기타 감염증은 통계적으로 유의한 차이가 없었다. 결론 : 간이식 환자에서 공여자의 종류에 따른 감염의 발생빈도는 차이가 없었으나 감염증 중 복강 내 감염은 LDLT에서 더 많이 발생하였다. 장구균 감염, 특히 반코마이신 내성 장구균이 간이식 후 발생하는 감염증의 중요한 원인 병원체임을 확인하였다. Background : Living donor liver transplantation (LDLT) has been performed more frequently to circumvent the shortage of donor organs. However, infectious complications after LDLT compared with orthotopic liver transplantation (OLT) have not been well investigated to date. Methods : We have retrospectively reviewed the medical records of all consecutive cases of liver transplantation, which had been performed at the Samsung Medical Center, Seoul, Korea, from May 1996 to August 2003. Results : Of the 284 patients enrolled, 188 (66.2%) underwent LDLT. Overall incidence of infectious complications after liver transplantation was 67.0% (191/284). Incidence of infections was not different between OLT group (67.7%) and LDLT group (67.0%) (P=0.9). Bacterial infections were the most common infectious complications (146 cases) followed by viral infections (32 cases), fungal infections (26 cases), and tuberculosis (4 cases). Enterococcus spp. (16.2%) were the leading pathogens followed by Staphylococcus aureus (15.1 %) and coagulase-negative staphylococci (13.4%). However, the distribution of etiologic agents was not different between OLT and LDLT group. Intra-abdominal infections (83 cases) were the most common type of infectious complications, which were more frequent in LDLT group (34.0%) than in OLT group (19.8%) (P=O.Ol). Conclusion : LDLT resulted in intra-abdominal infections more frequently than OLT. Enterococcus spp., especially vancomycin-resistant enterococci, are emerging pathogens in patients with liver transplantation.

자주달개비 미세핵 분석법을 이용한 작업환경내 총 먼지 중 수용성 추출물의 유전독성 평가

신해식,김진규,이재환,황갑성,김균,이정주,이진흥 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.6

In this study, we evaluated genotoxicity of heavy metals among particulates with Tradescantia-micronucleus (Trad - MCN) assay in the various working environment. In a synthetic fiber factory and a rubber factory, chromium concentration was higher than any other heavy metals. On the other hand, nickel concentration was the highest in a semi-conductor factory. The difference in genotoxicity among the working environment was statistically significant as Trad-MCN frequencies were 4.67±0.35 MCN/100 tetrads (p<0.01) for the synthetic fiber factory, 5.73±0.81 MCN/100 tetrads (p<0.0I) for the rubber factory, and 15.60±2.58 (p< 0.01) (p< 0.001) for the semi-conductor factory. As a result, heavy metals among particulates in the working environment can be considered to have hazardous potential to human health, although they cannot directly induce DNA damage to the workers in the working environments.

유리피판을 이용한 하인두-식도 재건 치험례

봉정표,정윤규,이훈범,김주봉,이재화,나동균,김석원 大韓成形外科學會 1997 Archives of Plastic Surgery Vol.24 No.6

Hypopharyngo-esophageal cancer is usually found at late stage. Its main treatment is surgical intervention and it often requires radical resection. Reconstruction of the hypopharyngo-esophageal defect after the resection of carcinomas of the hypopharynx and the cervical esophagus has traditionally been carried out with deltopectoral or musculocutaneous flap. Another approach is to reconstruct the defect with the colon or stomach. Recent advances in microvascular surgical techniques have resulted in one stage reconstruction with free flap. Both jejunal free flap and radial forearm free flap is a good alternative for the reconstruction of hypopharyngo-esophageal defect. Six cases of the radial forearm flap and nine cases of the jejunal flap were used to reconstructed the hypopharyngo-esophageal defect in 15 patients. In jejunal free flap, upper margin was reconstructed with end to side anastomosis and lower margin with end to end anastomosis. In radial forearm free flap, we used trapezoid shaped flap and tubed by complete or partial tubing according to the condition of the defects. Stricture and fistula are troublesome complications. The complications were peritonitis(n=1) and fistula(n=1). We concluded that both forearm and jejunal free flap is a useful alternative in reconstruction of hypopharynx and cervical esophagus.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

연쇄알구균 감염에 의한 급성 인후염 후 반응성 관절염 1예

박은하,도연실,양정채,강미라,서현주,정숙인,손준성,기현균,오원섭,백경란,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

연쇄알구균에 의한 급성 인후염 후 발생한 반응성 관절염은 급성 류마티스열의 개정된 진단기준을 충족하지 못하면서, 지속적 혹은 재발성의 비이동성 관절염과 발열, 홍반성 결절, 일시적 간기능 이상 등을 보이면서 심장염과 무도증을 드물게 동반하는 질환이다. 따라서, 발열을 동반한 급성 관절염 환자에서 감별해야 할 질환 중의 하나이며, 심근염 예방을 위한 페니실린 요법의 적응증 및 기간에 대해서는 아직 이론이 많은 상황으로, 보다 광범위한 연구가 필요하다. 저자들은 연쇄알구균에 의한 급성 인후염 1주 후에 발생한 하지의 다발성 비이동성 관절염과 함께 결절홍반 및 일시적 간기능 이상을 보인 환자에서 PSRA로 진단하고 비스테로이드성 항염증제 및 스테로이드로 성공적인 치료를 하였기에 이를 문헌 고찰과 함께 보고하는 바이다. Poststreptococcal reactive arthritis (PSRA) is associated with recent streptococcal infections. However, PSRA is distinct from acute rheumatic fever by its clinical manifestations: non-migrating arthritis, erythema nodosum or erythema multiforme, and transient elevation of serum transaminases. We experienced a 33-year-old man who presented with fever, arthritis of both knees and ankles, and erythema nodosums on extensor surfaces of lower legs which developed 6 days after the onset of pharyngitis symptoms. Blood and urine cultures were negative. Throat culture was negative for group A β-hemolytic streptococcus. The ASO titers increased up to 2080 IU/mL in sequential monitoring. The result of bone scan was compatible to arthritis of both knees and ankles. There were no signs or symptom of carditis. He showed clinical improvement with anti-inflammatory drugs (naproxen 1,000 mg/day and prednisolone 7.5 mg/d). PSRA should be included in the differential diagnosis of patients presenting with arthritis combined with fever.

Cloning and Characterization of Yeast Ribosomal RNA Promoter Binding Protein cDNA

Joo, Jae Hoon,Rho, Jae Kyun,Kim, Chul Geun,Choe, Soo Young,Park, Sang Dai Korean Society of Molecular Biology 1996 Molecules and cells Vol.6 No.4

Analysis for Regulatory Elements in Yeast MGMT Gene Transcription

Joo, Jae-Hoon,Kim, Woo-Jae,Rho, Jae-Kyun,Choe, Jae-Hyun,Choe, Soo-Young,Sang-Dai The Korean Society for Integrative Biology 1998 Korean journal of biological sciences Vol.2 No.2

The Saccharomyces cerevisiae MGMT gene encodes a O6-methylguanine DNA methyltransferase that protects cells from mutation or death by DNA alkylating agents. Using an in vitro transcription system, we analyzed its promoter region to find regulatory elements for transcription initiation. DNase I footprinting and a transcription assay showed that a functional TATA box, 5'-TGATATAGCA-3', is located in the region spanning from -25 to -34. We also found one upstream repressing sequence (URS), -333 to -213, by promoter deletion and competition analysis. Gel mobility shift assays and Southwestern blot analysis using URS region indicate specific complex formations. These results indicate that several cis-acting and trans-acting elements might be involved in the transcriptional regulation of the S. cerevisiae MGMT gene.

An Architecture and Service Model on National R&D Outcome Information

Won-Kyun Joo,Yong-Joo Shin,Ki-Seok Choi,Jae-Soo Kim 한국콘텐츠학회 2010 한국콘텐츠학회 종합학술대회 논문집 Vol.2010 No.12