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자주달개비 미세핵 분석법을 이용한 작업환경내 총 먼지 중 수용성 추출물의 유전독성 평가
신해식,김진규,이재환,황갑성,김균,이정주,이진흥 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.6
In this study, we evaluated genotoxicity of heavy metals among particulates with Tradescantia-micronucleus (Trad - MCN) assay in the various working environment. In a synthetic fiber factory and a rubber factory, chromium concentration was higher than any other heavy metals. On the other hand, nickel concentration was the highest in a semi-conductor factory. The difference in genotoxicity among the working environment was statistically significant as Trad-MCN frequencies were 4.67±0.35 MCN/100 tetrads (p<0.01) for the synthetic fiber factory, 5.73±0.81 MCN/100 tetrads (p<0.0I) for the rubber factory, and 15.60±2.58 (p< 0.01) (p< 0.001) for the semi-conductor factory. As a result, heavy metals among particulates in the working environment can be considered to have hazardous potential to human health, although they cannot directly induce DNA damage to the workers in the working environments.
자주달개비 미세핵 분석법을 이용한 비스페놀 에이 및 감마선의 생물학적 영향 비교 연구
신해식,송희섭,현성희,이진홍,김진규 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.2
합성화학물질의 몇몇은 고등동물의 내분비계를 교란하는 물질로 작용하는 것이 알려져 있다. 다이옥신,디디티,피시비 및 비스페놀 에이 등이 내분비계 장애물질로 분류되어 선진 각국에서는 엄격한 통제하에 관리되고 있다. 본 연구는 자주달개비 미세핵 분석법을 이용하여 염색체이상 유발에 있어서의 BPA와 방사선의 상대적 효과를 비교하기 위하여 시행되었다 흡수처리를 위하여 자주달개비 4430클론의 절취화서를 각각의 농도로 조제된 BPA용액에 여섯시간 동안 침지하고 폭기를 실시하였다. 한편 방사선의 영향을 평가하기 위하여 동일 식물체의 화서군을 양액에 침지한 상태로 O∼0.5 Gy까지 선량별로 조사하였다. 실험선량의 범위에서 미세핵 생성률은 분명한 선량-반응 관계를 나타내었으며 BPA 실험농도 범위에 대해서도 농도-반응 관계를 나타내었다. 이들 두가지 반응 관계식을 이용하여 동일한 미세핵 빈도를 유발하는 방사선량과 비스페놀 에이 농도를 산출할 수가 있는데, BPAll.8μM은 방사선 피폭후 임상증상이 나타나기 시작하는 선량인 0.25Gy에 상응하는 염색체 이상을 유발하는 것으로 확인되었다. 본 연구의 결과를 통하여 자주달개비 화분모세포는 비스페놀 에이, 욱틸페놀 또는 노닐페놀 등의 내분비계 장애물질의 생물학적 독성을 평가하는데 유용하게 활용할 수 있는 생물말단 점이라는 것이 입증되었다. Some of synthetic chemicals can act as an endocrine disrupting substance in higher animals. Dioxins, DDT, PCBs and bisphenol A (BPA) are classified into endocrine disruptors and are under a strict control in many countries. This research was designed to compare the clastogenic effects of BPA to those of ionizng radiation to establish the relative effectiveness of BPA by means of Tradescantia micronucleus assay. For the uptake of the BPA through the stems, groups of fresh cuttings of Tradescantia BNL 4430 were placed in BPA solutions of 0 to 4 ??M for 6 hours under continuous aeration. The other groups of the cuttings were irradiated with 0 to 0.5 Gy of gamma-rays. The frequencies of micronucleus showed a positive dose-response relationship in the range of 0 to 0.5 Gy, and a clear concentration-response relationship in the experimental range of BPA concentrations. By comparing the two experimental results, it is possible to estimate the BPA concentration and its equivalent radiation dose for a fixed value of MCN frequency. BPA of 11.8 ??M can give rise to 53.3 MCN/100 tetrads, which is the same frequency induced by 25 cGy of gamma-rays. It is of biological importance that clinical symptoms start to develop after a whole body exposure to radiation higher than 25 cGy. The results indicate that the pollen mother cells are an excellent biological end-point for toxicity test of suspected endocrine disrupting chemicals such as bisphenol A, cotylphenol and nonylphenol.
환경 중 유전독성물질 검색을 위한 자주달개비 생물검정 기법의 적용연구
신해식 환경독성보건학회 2004 한국독성학회 심포지움 및 학술발표회 Vol.- No.-
Higher plants can be valuable genetic assay systems for monitoring environmental pollutants and evaluating their biological toxicity. Two assays are considered ideal for in situ monitoring and testing of soil, airborne and aqueous mutagenic agents; the Tradescantia stamen hair assay for somatic cell mutations and the Tradescantia micronucleus assay for chromosome aberrations. Both assays can be used for in vivo and in vitro testing of mutagens. Since higher plant systems are now recognized as excellent indicators and have unique advantages over in situ monitoring and screening, higher plant systems could be accepted by regulatory authorities as an alternative first-tier assay system for the detection of possible genetic damages resulting from the pollutants or chemicals used and produced by industrial sectors. It has been concluded that potential mutagen and carcinogen such as the heavy metals among indoor air particulates, volatile compounds in the working places, soil, and water pollutants contribute to the overall health risk. This contribution can be considerable under certain circumstances. It is therefore important to identify the level of genotoxic activity in the environment and to relate it to the biomarkers of a health risk in humans. The results from the higher plant bioassays could make a significant contribution to assessing the risks of pollutants and protecting the public from agents that can cause mutation and/or cancer. The plant bioassays, which are relatively inexpensive and easy to handle, are recommended for the scientists who are interested in monitoring pollutants and evaluating their environmental toxicity to living organisms.
신해식,신동철 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2007 동물자원연구 Vol.18 No.-
The purpose of the study is to derive the problems and characteristics on channel of pig shipping by farm in Cheorwon Region that has 40% of pig feeding in Kangwon Province. Farmsurvey and interview with men of affairs were carried out for the study. As the result, individual farm shipping has more income among shipping channel in Cheorwon Region. In order to manage pig farmto developmore through creation of stable income and exchange the various andmuch information, corporate body shipping, namely pig brand project, is needed. Although the pig farmers intended joining the project positively, they avoid joining the brand project because there is a problemto decide feed farm. Cheorwon Region should invest to guide various education programs for technology developing and provide fiscal aid.
신해식 한국협동조합학회 2007 韓國協同組合硏究 Vol.24 No.2
Ⅰ. 서론 The purpose of the study is to seek plans of marketing revitalization for Hi-lok Hanwoo. Special counter for the Hi-lok Hanwoo brand meat has various plans for management according to approaching purpose. Thus, it is needed to set clear concepts for management of the counter. The Hi-lok Hanwoo brand sells the Hanwoo meat at special counters for the Hi-lok in Garakdong Livestock Joing Market and Hanaro Mart in agricultural cooperative. The function and role for the counter is very important to increase return at the special counters and restaurants for the Hi-lok Hanwoo brand meat.First, sales products and concept are headed for family restaurant that has lower prices and family menu. Second, marketing strategies are needed to make new customers. The consumers for Hanwoo boolgogi are very limited in eating out market at the present. The consumers must be varied to young college students, workers, and middle and old ages. Third, farmers of the Hi-lok Hanwoo and owners of the Hanwoo restaurant take the role to sale the Hanwoo meat. The farmer has difficulties to approach to the consumers who live in metropolitan. It limits the farmer role as suppliers. In order to increase farmer’s return the farmers must supply various food materials. Forth, the business purpose should be as follows. Restaurant that has a theme of Hanwoo boolgogi should be established. The restaurant develops various menus to increase value added using non-preferred part of Hi-lok Hanwoo, and analyzes consumer’s response through tasting. Commercial area should be analyzed to open the Hanwoo restaurants at zones that have huge floating population in Chunchon.