농산물중의 잔류농약 실태에 관한 연구(Ⅰ) : 고체상 추출에 의한 농산물중 잔류농약 분석에 관한 연구 Analysis of Residual Pesticides in Agricultural Products by Solid-Phase Extraction

허옥순,이재관,이정성,서정혁,주인선,허수정,김세은,김정근,신영희,유미자,김지연,심규창,김성환 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

신속 · 정밀하고 효율적인 짠류농약 검출을 위하여 극싱칼럼 (Extrelut-3')을 사옹한 SPE법을 시도하였다. 시료는 채소류체 사용하는 농약중 잔류성이 있고 식품공전상 시험 전처리가 각각 다른 Chlorothalonil등 6종의 농약을 선정하고 그들 표준액에 대한 PPIR수준의 농도로추출용라의 종류 및 용매량 등의 추출조건을 달리하여 회수율 등을 측정한 결과 최적의 분석조건을 얻었다. 1) 추출 용매량은 증가할수록 추출 수율이 높았고,용매 종류에 대한 추출수율은 각 농약별로 다소 차이는 있었으나 극성 정도가 비교적 높은 Ethyl acetate애서 평균 추출수율이 가장 높았다 2) 최대 평균회수율은 Ethyl acetate 60mL를 사웅할 경우로서 93.6%였다. 3)표준액을 시료에 첨가한 시험에서도 역시 Ethyl ace늘to가 푼출수율이 가장 높았으며 불순물 분리제거 효과도 LLE법 보다 월등히 우수하였다. 4) 각 농약별 검출한계는 치소 0.001ppin으로 농산물 중 미량 잔류하는 농약을 검출할 수 있을 것으로 사료된파. 그러므로 SPE법은 LLE법보다 경제적인 방법으로 판명되었으며, 앞으로 농산물 중 잔류농약 분석에서 LEE법을 대치할 쑤 있을 것으로 기대된다. Solid-phase extraction by polar column(Extrelut-3') was attempted to develope the fast and efficient method of detecting pesticide of farm product.5·ix kinds of pesticide used in farming fieldfrequently and different in pretreatment, were chosen from Korea Food Cord. Optfmal analysis condi-tions were determiBed from t31e recovery rate of standard pesticides according to extraction solvent andextraction volurae. Extraction yietd was increased as solvent polarity and extraction volume. Maximumrecovery rate was acquired at 93.6% when 60mL of ethyl acetate was used as eluent. Tllis method wasmore effective than liquid-liquid partition extract·ion method to eliminated the impurity and had 0.001ppm of detection limit. Therefore. solid-phase eEl=raction was expected to be economical substitute fortraditional liquid-liquid extT·action method.

"Tinidazole"(Fasigyn)에 依한 Giardia Lamblia의 驅蟲效果

崔源永,李玉蘭,李元求,이규면,朴定圭,李水月 中央醫學社 1973 中央醫學 Vol.25 No.4

"Tinidazole a new antiflagellate drug, was given orally for 3-7 days to 99 children infected with G.lamblia. Stool examination for detection and evaluation of therapeutic on giardiasis were made by formaline-ether concentration techniques. The children were grouped according to doses and the duration of administration of the drug. The results were summarized as follows; 1) Except 1 case out of 99 children, none of the children showed G. lamblia in the stool examination 2 weeks after administration of the drug, regardless of groups. 2) 4 weeks after administration of the drug, group A (10 mg per kg for 7 days) and group B (5 mg per kg for 7 days) children showed 100% of negative conversion, but group C (10 mg per kg for 5 days) children revealed 84.6% of negatives. 3) 6 months later, the negative conversion rates in groups A, B and C were reduced to 54.5%, 57. 1% and 50. 0% respectively. 4) Mild or transient side effects-nausea, abdominal pain, headache or fever and diarrhea were noted in 16 cases (27. 1%) out of 59 cases.

하루 콩단백질 25g 섭취를 위한 메뉴작성 및 영양성분 분석

한재숙,김정애,서봉순,이연정,서향순,조연숙,한경필,이신정,오옥희,우경자,조은자,구성자,김수진,李承彦,南出隆久 동아시아식생활학회 2002 동아시아식생활학회지 Vol.12 No.2

The purpose of this study was to develop menus for daily intake of 25g soybean protein and to analyse nutrients of these foods. Analytical values were compared to the theoretical one using the food composition table and recommended dietary allowances for Koreans (7th revision). The results are as follows. 1. Soybean curd residue stew, Soybean curd, Kimchi saute, and hard boiled soybean and lotus root were selected for the menu for January, of which the content of soy bean protein(SBP) was 33.1g, soybean stew, soybean curd and soybean sprout saute, and Italian deep fried soybean curd were for February, of which the content of SBP was 35.0g. The content of SBP in soybean paste soup with soybean curd, fried soybean curd and fried soybean curd roll, the menu for March, was 24.9g. That of April were soybean curd gratin with soymilk, soybean curd and ham with garlic dressing and the content of SBP was 26.3g. That of May were soybean porridge, soybean flour cake with honey (Dasik), soybean sprout soup and the content of SBP was 26.7g. That of June were soymilk, pan-fried soybean curd, steamed soybean curd with chicken and the content of SBP was 28.4g. That of July were noodle with soymilk, mapatofu, soybean curd salad and the content of SBP was 24.7g. That of August were soybean sprout with mustard dressing, Tossed green pepper with raw soybean flour, Tofu and Kimchi stew, soybean curd steak and the content of SBP was 26.2g. That of September were Chinese cabbage soup with raw soybean flour, sweet and sour tofu and the content of SBP was 23.2g. That of Oct. were Fermented soybean stew, soybean pan cake and the content of SBP was 24.3g. That of November were not-pressed soybean curd casserole, pan-fried mashed soybean curd with egg, stir frying deep-fried soybean curd with vegetables and the content of SBP was 22.4g. That of December were soybean curd and mushroom casserole, fried soybean curd and vegetables, hard boiled soybean curd and the content of SBP was 28.9g. 2. The ratio of the analytical value over theoretical value (A/B%) of one serving in kcal, carbohydrate, protein, fat, ash and dietary fiber were 57.7~107.7%, 42.9~131.9%, 79.2~118.3%, 54.5~100%, 40.7~80.8% and 42.1~113.2%, respectively. 3. The ratio of A/B% of one serving in Ca, K, Na, P and Fe were 44.1~93.6%, 59.0~153.1%, 53.1~117.7% 64.6%~138.8 and 33.8~77.3%, respectively. That in Fe was the lowest among minerals. 4. The ratio of analytical value over Korean R.D.A(A/C%) in Ca was relatively higher (22.0~85.9%) than that in kcal(18~63%). 5. The ratio of A/C% in Fe was 25.1~64.3% and lower than that in Ca and protein in general.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

학교체육 활성화 방안에 대한 연구(Ⅰ) : 강원대학교 교양체육 운영방법의 개선을 중심으로

이광재,문병용,노성규,박기동,홍관이,한상준,유옥재,엄기진,정청자,오수일,김윤래,박장평,부기원 江原大學校附設 體育科學硏究所 1986 江原大學校附設體育科學硏究所論文集 Vol.- No.11

Try to find a scheme for activation to College Physical Education, this Report informs the various kinds of Survey and process those were projected for improvement of management methods on Cultural Physical Education in Kang Weon National University. From experimentally carried out "Sports events Choosing System" not as usual be inforced "Total practical Skill System", Following Positive effects and sujestions were Presented; 1. "Sports events choosing system" gave birth to more students' interesting and more voluntary participation to the sports events they chose, and sports skill and instruction level was elevated due to charge of expert according to each sports events. This system would be managed continuously hereafter for it related to life sports. 2. This System needs to closed cooperations between College and Community, for the facilities of community are utilized by college. 3. To complete the plan for activation of college Physical Education, additionally, the successional study ; namely "autonomous extracurricular sports activity" should be excuted hereafter.

한국인에게 적절한 기관삽관 위치

이승한,최옥경,정구영,정성필 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Endotracheal intubation is one of the important procedures in ED and various fields. Endobronchial intubation can lead to hypoxemia secondary to tension pneumothorax of intubated side and atelectasis of contralateral side. The placement of tube beyond the vocal cords may results in inadvertent extubation, larygeal spasm and aspiration pneumonia. The conformation methods of endotracheal intubation are auscultation of the chest, observation of a vapor trail, oximetry and capnography, and radiologic evaluation. Owen and associates recommended that oral intubation tubes at the upper incisor teeth at the 23cm in men and the 21cm in women of average adult size would have led to proper placement. We studied that whether the proper depth of endotracheal tube in American is appropriate to Korean. We studied adult patients admitted to ED (of both hospitals during March in 1995. Endotracheal tubes in men were positioned at the 23cm and women had positioned at the 21cm at the upper incisor teeth. The distance of the tip of the endotracheal tube to the tracheal carina on the postintubation chest X-ray then determined. Correct placement of intubation was defined with the tip of the tube≥3cm above the carina and below the level of the larynx. Fifty adult patients were enrolled in the study ; 29 men (58%) and 31 women (42%). Mean height and weight were 170±5.2cm, 68±9kg in men and 158±6.5cm, 56±10kg in women. Reasons for intubation were ; respiratory failure(38%), trauma(16%), increased intracramial pressare(16%), drug intoxication(12%), shock(8%), cardiac arrest(7%)and others(3%). The mean distance from the tip to carina on chest X-ray was 4.13±1.5cm in men and 3.45±2.1cm in women. Proper position was 27/29(93%) in men, 14/21(67%) in women or 41/50(82%). this means the placement of endotracheal tube of our patients was positioned deeper than prion study group(97.6%).(p<.05) We recommend that the 22cm tube mark for men and 20cm tube mard for women at upper incisor teeth would have led to proper placement in Korean adult patient.

식용 버섯류가 흰쥐의 체내 지질 대사에 미치는 영향

이중희,최옥경 카톨릭대학 생활과학연구소 1987 생활과학연구논집 Vol.7 No.1

This study was designed to observe the effects of Shiitake(Lentinus edoders), Fungus (Pleurotus), Mushroom(Agaricus compestris), among dietary mushrooms on body lipld components in male rats. The results obtained in the study are summarized as follows: 1. Body weight gain and Feed Efficiency Ratio(F.E.R) of shiitake group were significantly lower than those of other experimental groups. 2. All the mushrooms used for feeding experiments significantly lowered serum cholesterol level, and the hypocholesterolemic effects of Shiitake (Lentinus edodes), and Mushroom(Angricus campestris) were more significant than that of Fungus(Pleurotus ostreatus). 3. Feeding of the mushroom also significantly decreased plasma triglyceride level and plasma HDL-cholesterol level. 4. There was a decreasing trend in the contents of liver cholesterol and triglyceride in the all group fed mushroom, however the differences between the experimental groups were not statistically significant.

광주 근교산 붕어의 아미노산 및 지질성분의 분석

李蓮玉,朴貞淑,金忠模,李炳燮,李明烈 조선대학교 기초과학연구소 1992 自然科學硏究 Vol.15 No.1

광주 근교산 붕어의 일반성분, total amino acid 및 free amino arid를 HPLC로 분석 그리고 유리지질과 결합지질을 추출하고 이를 각각 column chromatography에 의하여 중성지질, 당지질 및 인지질로 분획한 다음 이를 각 지질의 구성 지질을 TLC scanner, GC 등으로 분리 정량한 결과는 다음과 같다. 1. 붕어의 조단백질은 5.6%, 조지방은 5.4% 이었다. 2. 총 아미노산 및 유리아미노산은 17종이였으며, 총아미노산은 glycine이 많고 유리아미노산은 arginine이 많이 함유되어 있으며, 필수아미노산량은 총아미노산량의 약 28%를 차지하였다. 3. 총지질 중 유리지질이 5.2% 및 결합지질이 0.2%가 함유 되었으며 유리지질을 구성하는 지질의 함량을 중성지질 81.2%, 인지질 25.5%, 당지질 1.3%로 구성 되였으며 결합지질에는 중성지질 25.5, 인지질 70.0%, 당지길 4.5%로 구성되었다. 4. 유리 및 결합지길 중의 중성지질로는 monoglyceride, diglyceride, free sterol, fatly acid, triglyceride, esterified sterol 등이 분리 동정 되었으며 이중 triglyceride가 유리지질에 82.8%결합지질에 46.1%로 가장 많이 함유되었다. 5. 유리지질과 결합지질을 구성하는 인지질로는 phosphatidyl choline, lecithin, phosphatidylglycerol, phosphatidyl serine, steryl glycoside+phosphatidyl ethanolamine 등이 분리동정 되었으며 유리지질의 경우 phosphatidy1 choline이 2.8%, 결합지질에는 lecithin이 43.5%가 함유되었다. 6. 총지질을 구성하는 주된 지방산은 C_16, _OC18 1의 함량이 가장 많았으며 중성지질은 C_18 2의 함량이 높았으며, 당지질은 유리지질이 C_14 1, C_20 1이 다량 함유되어 있으며 결합지질은 C_20 4,C_18 1이 다량 함유되어 있다. 인지질의 지방산 조성은 C^19_0이 다량 함유되어 있으며 특히 결합지질에는 C_22 6이 다량 함유되어 있다. Approximate compositions, total amino acids and free amino acid, and the isolation and identification of fatty acids in Carassius auratus were analyzed by high performance liquid chromatography, thin layer chromatography and gas chromatography. The results are; Carassius auratus contained 73.8% water, 5.6% crude protein and 5.4% crude fat. 17 kinds of total and free amino acids were analyzed, respectively, glycin is the richest in total amino acids and arginine in free amino acids and total amounts of the essential amino acid were about 28% of total amino acids. Total lipid was contained 5.2% free lipid and 0.2% bound lipid. Free forms and bound forms were mostly consisted of neutral lipids(81.2%) and phospholipid(70.0%), respectively.

종속적 발전과정에서의 국가의 역할과 그 성격 : A Case Study on Korea, 1960~1980 1960~80 년의 한국의 사례를 중심으로

李貞玉 대구효성가톨릭대학교 1987 연구논문집 Vol.35 No.1