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      • 일록춘폐흡충(Paragonimus iloktsuenensis) 수용성 항원의 분자량 별 조직 항원성. : 1. SDS-PAGE 상에서 분리된 분자량 16~18kDa의 조직부위 별 항원성. 1. Tissue localities of molecular weight of 16~18kDa on SDS-PAGE

        이옥란 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.2

        This study was conducted to determine the worm tissue locality and antigenicity of 16~18kDa protein fraction separated from soluble crude antigen of Paragonimus iloktsuenensis. This fraction was obtained by SDS-PAGE, and regarded as the main antigenic protein fraction of D1 antigen seen in the early infection period (between 2~4 weeks of infection). After performing SDS-PAGE with soluble crude antigen, immune antibody (16~18-Ig) was prepared by immunizing gel antigenic emulsion(16~18A) of 16~18kDa protein fraction into a rabbit, and immune responses of worm tissues (tegument, intestinal epithelium, and vitelline gland) were observed using the immunogold labeling method. The results showed that 16~18kDa antigenic protein was present in all the tissues in which this protein was scarcely contained in the tegument, intestinal epithelium and vitelline gland. The antigenicity of intestinal tissue was significantly weak as compared with crude antigen(PIA). Although tissue antigenicity was not strong compared with the amount of protein, we believe that 16~18kDa antigenic protein was the main antigenic protein, being 64% of D1 antigenic protein and that the strong antigenicity of soluble antigen before the development of the vitelline gland(2 weeks before) was decided from this 16~18kDa antigenic protein originated from intestinal tissue.

      • KCI등재

        Efficient in Vitro Plant Regeneration from Hybrid Rhizomes of Cymbidium sinense Seeds

        이옥란,양덕춘,정해준,민병훈 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.3

        An oriental orchid that has leaves striped with different colors is considered to be one of the valuable hybrid cultivars. To propagate this hybrid cultivar, C. sinense Willd Makino (Kumwhasan, Bosei) and C. sinense Willd Makino (Seobo) were crossed. The crossed seeds were cultivated in vitro to induce rhizome development since rhizome-mediated propagation can shorten the long-term (by at least 6 years) propagation time and overcome variations induced by seedling propagation. Knudson and/or Hyponex medium with or without natural products were used for the rhizome and shoot induction. The optimum concentration of activated charcoal (AC) was 0.025% for the best shoot induction rate in line No. 115-1 where 1 mg・L-1 of NAA and 0.5 mg・L-1 of BA were added to the Hyponex medium and in line No. 115-2 where only natural products were added to the Knudson plus Hyponex medium. However, 0.2% of AC was shown to be optimal for line No. 118, which was grown on Hyponex medium without plant growth regulators.

      • Go Tell It on the Mountain에 나타난 John Grimes의 Identity 探求 : Go Tell It on the Mountain

        李玉蘭 동아대학교 인문과학대학 영어영문학과 1990 동아영어영문학 Vol.6 No.-

        Go Tell It on the Mountain is generally understood as one of James Baldwin's best novels as well as a major contribution to American fiction. The novel is divided into three parts. Part Ⅰ, "The Seventh Day", Part Ⅱ, "The Prayers of the Saints" and part Ⅲ, "The Threshing Floor" The novel deal with the Negro not as a social problem or as an oppressed minority, but concentes almost exclusively on one aspect of him and his way of life-the religious. The particular kind of religion which is grows out of his traditional situation in America and especially of his experirce of slavery. Baldwin renders the service with great skill which begins on Saturday eveninH and continues into the small hours. Through the Grimeses' prayers we learn the past lives of Gabiel, his wife, Elizabeth, his sister, Florence, and his step-son, John in a series of flashbacks : a whole intricate dramatic pattern of sin and guilt and repentance underlying their lives is revealed. And the novel ends with John's experience of conversion. The central event of this this novel is the religiour conversion of fourteen-year- old John but the truly major figure of interest is John's father, Gabriel- and it is Gabriel, chiefly around whom all the othe rcharacters' difficulties are centered. The writer endeavors here not only to interconnect the lives and psychology of all the characters but also to related these to the Southern Negro experience and the consequent shocks of urban slum living. As Gabriel's story is told through his memory, it is clear that "Father" and "Son" refer not only to God and Jesus Christ, but to the sons Gabriel fathers and rejects, adopts and hates, fathers and finds disappointing. In John, the innocent victim of Gabrie'l hatred, we have an, archetypal image of the Negro child. Baldwin sees the negro quite literally as the bastard child of American civilization. Obliquely, by means of an extended metaphor, Baldwin approaches the very essence of Negro experience. That essence is rejection, and its most destructitre consequence is shame. The flight from Negro self, the quest for identity, and the sophisticated acceptance of one's "blackness" is the theme that flow this emotion. That is, the wirter presents the Negro's inmost conflicts, his distress and his guilts from the past to the present and make attempt to discorer the meaning of Negro's subsistence and the possibilily of achieving Negor's identity as human being. In Part Ⅲ, "The Threshing Floor", as lohn lies before the altar, a series of visionary states passes through his soul. Images of darkness ane chaos, silence and emptiness, mist and cold. These images of damnation exprress the state of the soul when thrust into outer darkness by a rejecting, punishing, castrating father figure who is the surrogate of a hostile society. At the depth of John's despair, a sound emerges to assuage his pain. He had heard it all his life, but it was onlynow that his ears were opened to this sound that come from the darkness, that yet bore such witness to the glory of the light. And now in his moaning, he heard it in himself. It rose from his bledding, his cracked-open heart. It was a sound of rage and weeping from time set free, but bound noir in eternity. John's fear and guilt, and desire and despir and hatred have all been converted into a kind of meaningful delirium as he lies thrashing about the floor. On these harsh darkness and must forever share their pain. To the question, Who am I?, he can now reply : I am he who suffers and yet whose suffering on occasion is "from time set free". And thereby he discovers his humanity. We are very close to that plane of human experience where art and religion intersect. What Baldwin wants us to feel is the emotional pressure exerted on the Negro's cultural forms by his exposurc to white oppression. And finally to comprehend that these forms alone, through their power of transforming suffering, have enabled him to survive his tragic conditions of existence. Moreover, John's struggle on the threshing floor is described in terms of birth imagery, and the accomplished delivery set him free from the womb of childllood. That is, Baldwin anticipates in new -born John who seems to be a true Negro image of the new type in the futture, and also aspires to be an American society which the whites and the black can co-exist whithout the racial prejudice.

      • Immunoblot Technique을 이용한 간흡충증 진단에 관한 연구

        남해선,이옥란 순천향대학교 1990 논문집 Vol.13 No.1

        The experiments on Enzyme-linked Immunoelectro Transfer Blot(EITB) of the Clonorchis sinensis antigen and infected sera were carried out to find the specific antigenic component of the Clonorchis sinensis, and observe the antibody binding bands of the Clonorchis sinensis crude antigen(CAA) with clonorchiasis antisera as well as the cross immune reaction with paragonimiasis antisera. From the result of EITB between the clonorchiasis antisera and CCA, 14 antibody binding bands have came out. All of above, stronger antibody binding bands were ranged in molecular weight(MW) 45,000, 42,000, 38,000, 34,500, and 20,000 dalton. It was also consistent in major bands on SDS-PAGE of CCA. There was qualitative and quantitative difference of antibody in worm burden by EPG(egg per gram). In EITB between CCA and paragonimiasis antisera, there was the cross immune reaction in the range of MW 45,000, 42,000 32,000 and 20,000 dalton. The nine bands were appeared in EITB of CAA, and the MW of each band was consistent in the case of CCA. Also, the cross immune reaction with paragonimiasis antisera was occurred in the range of MW 45,000, 42,000, 32,000 and 20,000 dalton. In conclusion, it seemed that the antigenic bands with specific reaction on clonorchiasis were in the range of MW 38,000 dalton and 30,000∼25,000 dalton, but especially MW 38,000 dalton.

      • 천안 순천향병원 입원환자의 와포자충 난포낭 감염율

        이옥란,이선화 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

        From March 1998 to December 1999, 497 stool samples from inpatients with various disease in several departments of Chunan Soonchunhyang hospital were examined for the prevalence of Cryptosporidium. Stool samples were stained by a modified acid-fast technique for oocyst of Cryptosporidium. In results, Cryptospridium oocysts were found in 76 of 497 stools by modified acid-fast staining technique. The prevalenve was 15.29%. The prevalence of Chungcheongnamdo that hold to 91.35% of examined stools was 15.29%. The prevalence was 13.98%(33/236) in chunan, was 16.5%(36/218) in other chungnam areas except chunan. By age groups, the prevalence was more higher in sixties~seventies age groups than to that other age groups. By sex, the prevalence were more higher in male than female in age groups before forties, and were more higher in female in age groups after fifties. Consistency(diarrhea, semi-solid, solid) of stools were not associated with Cryoptosporidium positive rates. In conclusion, it was considered that the prevalence of Cryptosporidium was spreading in all over the chungcheongnamdo.

      • KCI등재

        In silico analysis of MeJA-induced comparative transcriptomes in Brassica oleraceae L. var. capitata

        이옥란,김대수 한국식물생명공학회 2016 JOURNAL OF PLANT BIOTECHNOLOGY Vol.43 No.2

        Brassica oleraceae var capitata is a member of the Brassicaceae family and is widely used as an horticultural crop. In the present study, transcriptome analysis of B. oleraceae L. var capitata was done for the first time using eight-week old seedlings treated with 50 μM MeJA, versus mock-treated samples. The complete transcripts for both samples were obtained using the GS-FLX sequencer. Overall, we obtained 275,570 and 266,457 reads from seedlings treated with or without 50 μM MeJA, respectively. All the obtained reads were annotated using biological databases and functionally classified using gene ontology (GO), the Kyoto Encyclopedia of Genes and Genomics (KEGG). By using GO analyses, putative transcripts were examined in terms of biotic and abiotic stresses, cellular component organization, biogenesis, and secondary metabolic processes. The KEGG pathways for most of the transcripts were involved in carbohydrate metabolism, energy metabolism, and secondary metabolite synthesis. In order to double the sequenced data, we randomly chose two putative genes involved in terpene biosynthetic pathways and studied their transcript patterns under MeJA treatment. This study will provide us a platform to further characterize the genes in B. oleracea var capitata.

      • 미국 黑人像 硏究 : 백인 작가들의 소설을 중심으로 Based on Novels of White Authors

        李玉蘭 新羅大學校 1995 論文集 Vol.39 No.1

        This study aims to pursue the black portraiture as manifested in American fiction written by white authors. Through an examination of forty novels and short stories written during the period 1820s to 1970s, which deal with black figures, the development of the delineation of the black image by white American authors is explored. An attempt is also made to trace how the black characters develop from the stereotype to the black individual by focusing on how cultural myths and attitudes have influenced their portraiture though an analysis of these works. In order to assess the patterns and trends black portraiture, it has been necessary to examine characters contained in a wide range of nineteenth and twentieth century popular literature. The sample that has been drawn, therefore, has not been limited to artistic works. Based on an analysis of fictional images of blacks encountered in these forty works, can be seen : four major configurational patterns : the contented slave image : the brute Negro : the primitive image : and the black individual. Firstly, in chapterⅡ, in works by white authors written before and after the emancipation, many black slaves were described in a way that fit the image of the contented slave who refused freedom when it was offered him, preferring to remain a faithful, protective custodian of his master's physical welfare, rather than becoming master of his own destiny as a free man. The delineation of these characters reflect the cultural belief that Negroes are inferior to and dependent upon white, who assume responsibility for their welfare. Secondly, in chapterⅢ, after the Civil War the docile, contented slave image gave way to the "brute Negro" stereotype. Especially in novels written by Southern white authors from the post-war period well into the twentieth century the concept of black savagism and inferiority is promulgated. In the works of these Southern writers appear numerous examples of the "brute Negro" who, once freed, is transformed from a state of happiness under slavery, into a brute who terrorizes the land and rapes white women. The intention of such depictions is to convince the reader that black people are basically savage, animalistic creatures who thus deserve a less than human existence. Thirdly, in chapterⅣ, another persistent archetype is analyzed : the image of the primitive black. This manifests itself in several way : as the nobel savage who is unaffected by contacts with Western culture : as the atavist who reverts to jungle primitivism : as the carnal sensualist whose whole being is dominated by violent passions. These novels' controlling notion is that the white world, if it is to remain alive, must reconnect with the physical element of life, including sexual fulfillment. And many white writers felt this revivication could be found best through contact with black culture. So they produced various kinds of primitive black stereotypes. In addition to the stereotypes and simplification, these kinds of writings show less interest in black people than in black life as symbolic of contrasting values and life styles. If their authors deny their rich and vivid fictional lives, these people once again become slave to white culture. Lastly in chapterⅤ, this study analyzes the image of the black individual. In chaptersⅡ-Ⅳ, changes in cultural attitudes toward black have been evidenced in shifting modes of fictional characterization. Although changes occurred, some portraits still carried strong overtones of earlier cultural attitudes. Among the characters analyzed in chapterⅤ can be found changes from portraits that contain traces of earlier attitudes to those that depict blacks who have become or are in the process of becoming individuals, each possessing a strong sense of identity and independent responsibility. Those portraits containing evidence of earlier cultural attitudes toward black can be classified as individuals in transition from subservience to self-assertion.

      • KCI등재

        흑인여성의 사랑의 비극 : 토니 모리슨의 『빌러브드』 Toni Morrison`s Beloved

        이옥란 한국현대영미소설학회 1996 현대영미소설 Vol.3 No.-

        This study aims to pursue how Toni Morrison, in her most outstanding novel, Beloved, explores the healing process of the closed consciousness of the Black-Americans who have undergone suffering resulting from the trauma of their slave experience, and how she searches out the way to recover their communal identity. In this novel, as in her other works, Morrison pursues various inner aspects of the black community that black male writers have not been conscious of or have passed over. By inquiring into Sethe's painful inner state of mind, who feels she has to kill her baby daughter, Morrison extends Sethe's personal problem to the problems of the black community. Both a slave and a woman, she makes up her mind that her daughter will not suffer the way that she has, and that it is better for her to die. In other words, this is a tragedy resulting from excessive maternal affection. Sethe realizes that she has killed her daughter because of her own experience, but she cannot free herself from the painful past with its remorse, grief and sorrow. Then Beloved, the incarnation of Sethe's daughter, appears, and forces Sethe to squarely face a past which she can neither remember nor forget. In thus boldly facing the past by her "rememory," she can overcome it and look forward to the future. In the same way, the inability of the former slaves to free themselves from the past is due to their failure to speak out their painful past. For them Beloved plays an important role as catalyst. Therefore, Beloved is not only the incarnation of Sethe's deceased daughter, but the representative being of "Sixty Million and more" who have died namelessly and unjustly. Morrison says, "This is not a story to pass on," but it seems to mean paradoxically that "this is not a story to forget," because by not forgetting, by remembering the past, knowing it and facing it squarely, one can free oneself from the chain of the past. Considered as a metaphor of the human condition, the Black-American's experiences are not confined to the Black-American only, but can be extended to human beings in general.

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