The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

제대혈 단핵구 냉동보존 기법에 따른 해동 후 세포 회복률 비교 : 급속 냉각 vs 통제속도 냉각 Dump Freezing vs Controlled Rate Freezing

오상철,김병수,윤수영,박경화,윤소영,김석진,서재홍,최철원,신상원,김열홍,김준석 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

연구배경: 저자 등은 조혈모세포를 -80℃ 냉동고에서 급속냉각법을 통하여 경제적으로 보존하는 방법의 냉동보존 효율이 통제속도냉각 후 액체질소보존법의 냉동보존 효율과 동등한 유효기간을 규명하고자 본 연구를 시행하게 되었다. 방법: 총 20 명의 산모에서 채집한 재대혈을 대상으로 하여 10% DMSO로 제대혈을 -80℃로 급속냉각한 경우(실험군)와 통제속도냉각장치로 냉각 후 액체질소냉동고에서 보존한 경우(대조군)에서의 해동 후 세포 회복률 및 기능의 회복 정도가 동등한 기간의 범위를 파악하기 위하여 3달 간격으로 총 18개월 동안 각 군 간의 해동 후 단핵세포회복률, CFU-GM 집락회복률, CD34+ 세포회복률 등을(평균)±(표준편차)로 표시하여 Paired t test를 통하여 비교, 분석하였다. 결과: 1) 생존 단핵세포 회복률: 대조군(통제속도냉각군)과 실험군(급속냉각군)을 비교하여 보았을 때, 모든 기간 동안 각 군 간에 유의한 차이를 발견할 수 없었다. 2) CFU-GM 집락 회복률: 대조군(통제속도냉각군)과 실험군(급속냉각군)을 비교하여 보았을 때 12개월 이후부터는 대조군이 실험군에 비하여 높은 경향의 회복률을 보였으나 양 군 간의 유의한 차이는 없었다. 3) CD34+세포 회복률: 대조군(통제속도 냉각군)과 실험군(급속 냉각군)을 비교하여 보았을 때 12개월 이후부터는 대조군이 실험군에 비하여 높은 경향의 회복률을 보였으나 양 군 간의 유의한 차이는 없었다. 결론: 제대혈에서 채집된 단핵구를 냉동보존 시 18개월까지 -80℃ 급속 냉각법으로 보존한 군과 통제속도 냉각 후 액체질소 보존 군 간에 단핵세포 회복률, CD34 양성 세포 회복률, CFU-GM 세포 회복률의 유의한 차이가 없었음을 관찰할 수 있었다. Background: This study was designed to compare the mononuclear cell recovery rate of cord blood after thawing and freezing according to cryopreservation methods: dump freezing vs controlled rate freezing. Methods: Twenty samples of cord blood were divided into dump freezing and controlled rate freezing groups. The viable mononuclear cell, CFU-GM and CD34+ cell recovery rates were evaluated at the time of post-thawing 1, 3, 6, 9, 12, 15 and 18 months after cryopreservation with 10% DMSO and compared between two groups. Results: The mean viable mononuclear cell recovery rates from cryopreservation 1 month to 18 months of dump freezing and controlled rate freezing were 81~88% and 82~89%, respectively. There was no statistical difference between two groups. The mean CFU-GM colony and CD34+cell recovery rates of dump freezing and controlled rate freezing (cryopreservation duration: 1 month to 18 months) were 66~76% vs 71~78% and 86~95% vs 86~94%, respectively. There was no statistical difference between the results of two groups. Conclusion: The -80℃ storage without rate-controlled freezing of cord blood was practically applicable with satisfactory cell recovery rates after thawing, which was comparable with those cryopreserved with controlled rated freezing and liquid nitrogen storage.

Aromatic toxicants에 의해 유도된 간기능 장해에 미치는 acorn pollen의 영향

윤수홍,박은주 대구효성가톨릭대학교 1991 연구논문집 Vol.43 No.1

Although there are many drugs used in treatment of liver diseases, there effectiveness is very often unsufficient and questioned. Search for new drugs and methods of pharmacotheraphy of liver damage, the acorn pollen extract was administrated to the experimental animals and investigated its effects biochemically in the serum and liver when aromatic hepatotoxicants-phenyl-butazone, aniline and benzo(a)py-rene-was administrated. As the results are; 1. 8 kinds of minerals were quantitatively analyzed at acorn pollen and major components of them were potassium and calcium. 2. 16 kinds of fatty acids were quantitatively analyzed and major components were linoleic acid and linolenic acid. 3. The amino acid of acorn pollen contained 17 kinds and especially aspartic acid and glutamic acid. 4. Acorn pollen pre-administrtion prevented the increase of serum and liver ALT, AST, ALp activity and bilirubin contents caused by hepatotoxicants. But acorn pollen was not effectiv on serum LDH activity and AST activity caused by phenylbutazone.

體育科 Mat運動의 能力別 學習 適用效果에 關한 硏究

洪盛杓,陣潤洙 忠南大學校體育科學硏究所 1984 體育科學硏究誌 Vol.2 No.1

This study was conducted to see the application effect of learning by ability in Mat exercise. 367 Male middle school students who were second grade were served as subjects. They were divided into control group and experimental group. Control group was hetrogeneous group and experimental group was organized group by ability. After 8 hours' learning of Mat exercise, their motor performance were compared. The following conclusions were obtained; 1. In teaching of Mat exercise in Male middle school, group-organization by ability was more effective than hetrogeneous group-organization. 2. In stepped and systematic teaching materials such as Mat exorcise , effective motor skill improvement is possible through leaching method of group - organization by ability. 3. Especially in the learning of difficult motor events and motor events which need repeated practice and which lack of pre-learning, application effect of learning by ability is big.

Benzo(a) pyrene에 의해 유도된 간기능 장해에 미치는 시호와 황금의 병용 효과

尹水弘,吳官絃,朴銀珠 대구효성가톨릭대학교 1992 연구논문집 Vol.45 No.1

This study was attempt to evaluated the effect of Bupleuri Radix extract and Scutellariae Radix extract as the hepatotropic agents. In addition, we measured the relative effectiveness of drug combined treatment in comparison to that of alone treatment. The results are as follows ; 1. Serum and liver AST, ALT activities and liver bilirubin content of B(a)P treated group were significantly increased in comparison to normal group. After administration of Bupleuri Radix extract these enzyme activity and bilirubin content were decreased. 2. Scutellariae Radix extract decreased the increase of serum and liver AST, ALT, liver LDH activities and bilirubin content in B(a)) treated group. 3. After combined treatment of Bupleuri Radix and Scutellariae Radix extract, the serum and liver AST, ALT activities and liver bilirubin content were significantly decreased in comparison to that of drug alone treatment. 4. Buplerui Radix extract prevented the leakage of serum and liver total cholesterol, serum phospholipid and triglyceride levels caused by B(a)P treatment. 5. Scutellariae Radix extract prevented the leakage of serum and liver triglyceride and liver total cholesterol levels caused by B(a)P treatment. 6. After combined treatment of Bupleuri Radix and Scutellariae Radix extract, the lipid accumulation of serum and liver was significantly decreased in comparison to that of drug alone treatment. 7. The hepatotropic effect of drug extracts against B(a)P induced hepatotoxicity was superior to combined treatment of Bupleuri and Scutellariae Radix extract and after-treatment in comparison to that of alone treatment and pretreatment.

천연자원의 간기능 보호 효과에 관한 연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 강활의 효과 The Effect of Angelica koreana on Benzo(a)pyrene Induced Hepatotoxicity

윤수홍,이송애,박은주,이주영 효성여자대학교 부설 한국환경위생연구소 1992 환경위생연구 Vol.2 No.1

민간에서 주로 사용되는 약용식물들 중 간독성의 발현 저해 및 간기능 보호 효과가 우수한약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험과정으로 탄소화물의 불완전 연소 및열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의 간독성 발현에미치는 강활 수침액의 예방 및 치료 효과를 실험하여 다음과 같은 결과를 얻었다. 1. 강활 수침액의 투여는 B(a)P투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH,ALP 활성의 유의성 있게 감소시켰다. 2. B(a)P투여로 증가한 혈청 total cholesterol 및 phospholipid 함량은 강활 수침액의 투여로감소시킬 수 있었다. 3. 강활의 투여는B(a)P에 의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가후처리에 비해 우수하였다. The present study was undertaken to compare the pharmacologicalactivities of crude Angelica koreana reported with the clinical uses in the orientalmedicine. Crude angelica. used for the treatment of common cold, headache, neu-ralgia and arthralgia. Therefore we tested the effects of Angelica water extract on the liver-protec-live activities in the rats. The results obtained from liver microsomal enzymeassay, measurement of serum and liver alanine' aspartate aminotransferase andlipid accumulation indicated that angelica extract showed significant liver-protec-tive activities against benzo(a)pyrene poisoning.

Benzo(a)pyrene에 의해 유도된 간기능장해에 미치는 자근 추출액의 영향

윤수홍,박은주,오관현,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1993 환경위생연구 Vol.3 No.1

민간에서 주로 사용되는 약용식물들 중 간독성 의 발현 저해 및 간기능 보호 효과가 우수한약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험과정으로 탄소화합물의 불완전 연소및 열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의 간독성발현에 미치는 자근 수침액의 예방 및 치료 효과를 실험한 결과 자근 수침액의 투여는B(a)P 투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성있게 감소시켰고 B(a)P 투여로 증가한 혈청 total cholestelof 및 phospholipid 함량 역시감소시킬 수 있었으나 그 효과는 현저하지 않았다. 즉, 자근 수침액의 투여는 B(a)P에의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하였다. The present study was undertaken to compare the phamacologicalactivities of crude Lithospermi Radix reported with the clinical uses in the orientalmedicine. Crude Lithospermi Radix uesd for the treatment of burn, eczema,blister, diuretic, scarlet fever and septicemia as antipyretic, antidotic and antiph-logistics. Therefore we tested the effects of Lithospermi Radix water extracton the liver-protective activities in the rats. The results obtained from enzymeassay, measurement of serum and liver alanine aspartate aminotransferase(ALT,AST) and lipid composition indicated that Lithospermi Radix water extract sho-wed significant liver-protective activities against benzo(a)pyrene-induced hepato-toxicity.

Benzo(a)pyrene에 의해 유도된 간기능장해에 미치는 자근 추출액의 영향

윤수홍,박은주,오관현,정영건,권오진 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

민간에서 주로 사용되는 약용식물들 중 간독성의 발현 저해 및 간기능 보호효과가 우수한 약물을 찾아 임상적인 약효를 밝혀보고자 하는 실험 과정으로 탄소화합물의 불완전 연소 및 열분해에 의해 생성되는 간장해 물질인 benzo(a)pyrene 으로 유도한 rats 의 간독성 발현에 미치는 자근 수침액의 예방 및 치료효과를 실험한 결과 자근 수침액의 투여는 B(a)P 투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성있게 감소시켰고, B(a)P투여로 증가한 혈청 total cholesterol 및 phospholipid 함량 역시 감소시킬 수 있었으나 그 효과는 현저하지 않았다. 즉, 자근 수침액의 투여는 B(a)P에 의한 간독성 발현을 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하였다. The present study was undertaken to compare the pharmacologicalactivities of cn de Lithospermi Radix reported with the clinical uses in the orientalmedicine. Crude Lithospermi Radix uesd for the treatment of burn, eczema,blister, diuretic, scarlet fever and septicemia as antipyretic, antidotic and antiph-logistics. Therefore we tested the effects of Lithospermi Radix water extracton the liver-protective activities in the rats. The results obtained from enzymeassay, measurement of serum and liver alanine·aspartate aminotransferase(ALT, AST) and lipid composition indicated that Lithospermi Radix water extractshowed significant liver-protective activities against benzo(a)pyrene-induced he-patotoxicity.

Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 쑥의 효과

윤수홍,조수열,박은주,김성중 한국환경독성학회 1992 환경독성보건학회지 Vol.7 No.1

Mugwort has been used as a Korean folk medicine in treating liver diseases acting as an analgesics, sedative, diuresis, choleretics. This study was perfomed to evaluate the effect of mugwort extracts on the changes of enzyme activities, lipid accumulation of the serum and liver, when hepatotoxicity was induced by benzo(a)pyrene. The results are as follows: 1. Mugwort water extract administration prevented the increase of serum and liver AST, ALT, LDH, γ-GTP, liver ALP activities and bilirubin content caused by B(a)P injection. 2. The increase of serum and liver ALT, LDH, γ-GTP, serum AST activities and liver bilirubin contents in B(a)P treated group were decreased by mugwort methanol extract treatment. 3. Serum and liver total cholesterol, phospholipid, triglyceride level and serum HDL-cholesterol level were increased by B(a)P treatment. After combined treatment of mugwort water and methanul extracts, these lipid content were significantly decreased. 4. The hepatotropic effect of mugwort water extract and after-treatment against B(a)P induced hepatotoxicity was superior to that of methanol extract and pretreatment.