有機燐系 殺蟲劑가 흰쥐의 Cholinesterase 活性에 미치는 影響

윤수홍,김신희,박병윤 한국환경독성학회 1987 환경독성보건학회지 Vol.2 No.1

본 硏究는 흰쥐에 有機燐系 농약인 malathion과 DDVP의 投與에 의한 血淸, 腦 및 肝臟의 ChE活性, 電氣泳動에 의한 ChE isozyme pattern 및 病理組織學적 상태의 변화를 조사하였다. 血淸, 腦 및 肝臟의 ChE활성은 malathion과 DDVP 投與群에서 농도와 시간이 증가함에 따라 저하하였으며, 血淸 및 腦 ChE활성은 7일째는 저하하였으나 시간이 경과함에 따라 다소 회복되는 경향을 나타내었다. ChE isozyme은 血淸 및 肝臟에서는 전기영동상 많은 band가 관찰되었고 腦에서는 소수의 band만이 나타났으며 농약의 투여 농도 및 시간의 경과에 따라 band의 수와 선명도가 감소되었다 肝臟의 病理組織學적 檢査는 malathion 投與群에서는 central vein과 portal area사이의 midzonal necrosis가 관찰되었으며, DDVP 投與群에서는 portal area에 국한된 portal necrosis가 주된 所見이었다. Changes in cholinesterase (ChE) activity, electrophoretic pattern of ChE and histopathologic state on the mice serum, brain and liver by administration of organophosphorous insecticides were studied. The mice ChE activities on serum, brain and liver decreased by increasing of concentration and time both administration of malathion and DDVP, whereas on serum and brain the activities of the 7 days after administration decreased, and then presented the gradually slight recovery in course of time. The ChE on serum and liver showed many isozyme bands by polyacrylamide gel electrophoresis but several on brain. And isozyme bands disappeared and diffused by administration of organophosphorous insecticides and development of time. The mice liver with administration group of malathion on histopathologic test showed midzonal necrosis between central vein and portal area, and with administration group of DDVP mainly presented portal necrosis on location of potal area.

일부 초등학교 학생들의 책상 및 의자상태와 이용자세에 따른 신체증상에 관한 연구

윤수홍,박상래,안형자 한국위생과학회 1998 한국위생과학회지 Vol.4 No.1

This study was conducted to investigate the physical symptoms by postures and conditions of used chairs and desks for 515, 6th-grade students in an elementary school in Koryung-kun, Kyeongsangbukdo from December 20, 1997 to March 30, 1998. Children who have experienced trunk and low back pain were the higher 44.1%, low extremities 29.1%, neck 27.8%, upper extremities and shoulders 19.6%, gluteal pain 14.7% in the physical symptoms by using chairs and desks in class. Children(10.7%) who sat uprightly were lower than the others(71.8%) in a posture seated an the chair. Forward hyperflexion was the highest 63.8%, backward hyperextension 21.9%, left and right bending 11.9% in the reason why didn't sit in a correct posture. In the distribution of a posture seated an the chair by gender, boys 72.7% that answer a wrong posture were similar to girls' 70.9%, girls forward hyperflexion 69.1% was higher than boys' that 58.9%, boys' backward hyperextension 29.2% was higher than girls' that 14.0%, and girls' left and righ lateral flexion 14.0% was a sightly higher than boys' that 9.9% in the reason of wrong postures. There were upper extremities and shoulders(P=0.001), trunk and low bask pain(P=0.001) and low extremities pain(P=0.001) in the significant variables associated with height of desk compare to pain part of bodies. Children who answered low(each 34.7%, 33.9%, 46.7%) were higher than that of high(7.9%, 3.5%, and 7.3%). The significant variables related to the height of chairs and physical symptoms were neck(P=0.001), upper extremities and shoulders (P=0.003), trunk and low back(P=0.001), gluteus(P=0.001), and low extremities(P=0.001). Children' s physical symptoms rates are 34.3%, 34.6%, 33.0%, 40.8%, and 37.3% that answer low were higher than those of others' 5.6%,6.9%, 4.8%, 1.3%, and 8.0%, each others. The relationship between physical symptoms and seatpan, trunk and low back pain(P=0.006), gluteal pain(P=0.001) were significant variables. Broad in seatpan condition 11.4% was similar to narrow 11.0% in trunk and low back pain. Narrow in seatpan condition 25.0% was higher than broad 9.2% in gluteal pain. Trunk and low back pain was significantly associated with a posture seated an the chair(P=0.025), from Children' s 77.9% that answer a wrong posture.

農家所得增大를 위한 보리쌀 消費增大方案에 관한 硏究 : 보리쌀 炊飯特性 硏究를 통한 보리 利用性 提高方案

尹水弘,徐挺植,金尙玉,玄仁煥 영남이공대학 1981 論文集 Vol.10 No.-

This study was carried out as a program to increase the utilization of barley by improving the acceptable eating quality of Korea's traditional cooked barley of a grain type. The barley's characteristics of cooking and acceptable eating quality according to its pearling rate by pearling it differently in the four classes; 70%, 60%, 50%. 45% (as the weight ratio of pearled barley to covered one) were examined extensively including the general compositions of samples, the time course changes of barley's volume and weight in proportion to the soaking before cooking, the experiments of the physiochemical characteristics (the water-uptake ratio and swelled volume of polished barley when cooked, the pII, iodine color intensity and total solids in the residual cooking liquid) and textural properties by the universal testing machine about the cooked barley as a objective testing method of acceptable eating qualities, the sensory evaluation of cooked barley as a subjective testing method of acceptable eating qualities, etc. The results were as follows: 1. According to the decrease of pearling rates, moisture contents and nitrogen-free extracts of samples were increased slightly, but their components of crude protein, crude lipid, crude fibre and ash were decreased greatly 2. In the time-course changes of the capacity of water-uptake and swelling of volume according to the soaking of samples before cooking, the increased weight ratios were increased in proportion to the decrease of pearling rate of barley, but the increased volume ratios were increased according to the order of covered barley, Akibare(rice), 50% pearled barley, 60% pearled barley, 70% pearled barley, 45% pearled, ane especially the volume increases were marked at the early stage of soaking in 70% pearled barley. 3. According to the decrease of pearling rate, water-uptake ratios during cooking were increased, but swelling of volume had the maximum value at 60% pearled barley, decreased again having the minimum value at 45% pearled barley. 4. pH values of the residual cooking liquid of the cooked barley were decreased according to the decrease of pearling rate and were considerably less than the pH, 6.46 of Akibare. The iodine color intensity and total solids of the residual cooking liquid of the cooked barley were increased greatly according to the decrease of pearling rate, but were considerably lower as compared with those of Akibare as a whole. 5. The texture profile of the cooked barley obtained by the universal testing machine were analyzed by the methods of Bourne. Henry etc. Max. force known to hardness, force to 20% compression, compressive gumminess, compressive chewiness were increased according to the order of pearling rate, 60%, 50%, 45%, 70% and recorded the higher values as compared with those of Akibare. Compressive cohesiveness, peak force, tensile hardness, tensile springiness, tensile cohesiveness, tensile gumminess, tensile chewiness were increased according to the decrease of pearling rate of barley and reached the maximum at 45% pearled barley, but recorded the much lower values as compared with those of Akibare. But springiness did not show some differences between the pearling rates or rice and barley polished. 6. According to the results of panel tests about the cooked barley by the hedonic scoring method based on a 5-point scale, color, tasts, appearance, adhesiveness, total acceptability had significant differences between samples at 95% level, but other quality factors did not and there were no significant differences between panel members. The means of total scores increased according to the order of pearling rate. 60%. 70%, 50%, 45%, and therefore it was shown that 45% pearled barley had the best acceptable eating quality.

일차 배양한 간세포에서의 권백의 간보호효과의 Screening Test

윤수홍,이주영,하헌 한국환경독성학회 1996 환경독성보건학회지 Vol.11 No.2

Selaginellae Herba has been used as folk medicine for antineoplastics, coagulants, antidotes and invigorants. To find an in vitro screening method for liver protective effect of Selaginellae Herba in benzo(a)pyrene intoxicated injury were examined in primary cultured rat hepatocytes. Using MTT assay, herba concentration showed dose dependently viability. The lowest concentration of benzo(a)pyrene giving cytotoxicity revealed around 50μM. The hepatoprotective effect of Selaginellae Herba in both water and chloroform extracts was also increased dose dependently.

흰쥐에 2-Bromopropane 의 급성 독성에 관한 연구

윤수홍,차상은,박상래,최정숙 한국위생과학회 1996 한국위생과학회지 Vol.2 No.1

2-Bromopropane(2-BP), important industrial chemical, specially in electronics industry has been reported to cause menstrual irregularity and anemia for female workers in Korea. The present study was undertaken to characterize the toxicity of 2-BP, following acute intraperitoneal 200, 400, 600 and 800mg/kg injection of Sprague-Dawley rats once a day in four consecutive days. WBC, MCV and MCH in 2-BP treated rats were no difference among concentration levels, including serum ALT, γ-GTP and total protein level of liver.

랫드 일차 배양 간세포에서 사염화탄소의 독성에 대한 지잠 물추출물의 보호효과

윤수홍,김덕현,현선희,이상규,전태원,정태천,Yun, Soo-Hong,Kim, Duk-Hyun,Hyun, Sun-Hee,Lee, Sang-Kyu,Jeon, Tae-Won,Jeong, Tae-Cheon 대한약학회 2006 약학회지 Vol.50 No.4

Protective effects of the water extract of Protaetia brevitarsis larva against $CCl_4-induced$ toxicity were investigated in primary cultures of adult rat hepatocytes. The extract used in these studies contained several minerals, fatty acids and amino acids. Treatment of hepatocyte cultures with the extract provided a significant protection from the increased LDH activity induced by $CCl_4$. The results demonstrated that the extract may have the protective effect against $CCl_4-induced$ toxicity in hepatocyte cultures.

실험적 간장해에 대한 강활의 보호효과

윤수홍,하헌 환경독성보건학회 2005 환경독성보건학회지 Vol.20 No.2

일차 배양한 간세포에서의 권백의 간보호효과의 Screening Test

윤수홍,이주영,하헌,Yoon, Soo-Hong,Lee, Ju-Young,Ha, Hun 환경독성보건학회 1996 환경독성보건학회지 Vol.11 No.3

Selaginellae Herba has been used as folk medicine for antineoplastics, coagulants, antidotes and invigorants. To find an in vitro screening method for liver protective effect of Selaginellae Herba in benzo(a)pyrene intoxicated injury were examined in primary cultured rat hepatocytes. Using MTT assay, herba concentration showed dose dependently viability. The lowest concentration of benzo(a)pyrene giving cytotoxicity revealed around 50gM. The hepatoprotective effect of Selaginellae Herba in both water and chloroform extracts was also increased dose dependently.

감광성 염료를 도핑한 고분자 태양 전지 소자 연구

윤수홍,박재우,허윤호,박병주,Yun, Soo Hong,Park, Jae Woo,Huh, Yoon Ho,Park, Byoungchoo 한국전기전자재료학회 2013 전기전자재료학회논문지 Vol.26 No.3

We introduced sensitizing dyes into the bulk-heterojunction (BHJ) photovoltaic (PV) layer of polymer solar cells (PSCs). The sensitizing dyes doped were Bis(tetra butyl ammonium) cis-dithio cyanato bis(2,2'-bipyridine-4-carboxylicacid-4'-carboxylate) ruthenium (II) (N719 dye) and the BHJ PV layer used was made of poly (3-hexylthiophene) (P3HT) and phenyl $C_{61}$-butyric acid methyl ester (PCBM). It was found that the N719 dyes increase the photovoltaic performance, i.e., increasing open-circuit voltage and short-circuit current density with improved fill factor. For the P3HT:PCBM PV cells doped with the N719 dyes (0.24 wt%), an increase in power conversion efficiency of 4.0% was achieved, compared to that of the control cells (3.6%) without the N719 dyes.

사염화탄소에 의해 유도된 흰쥐의 간장해에 미치는 지잠의 보호효과

윤수홍,김덕현,이상규,김주현,서영민,강미정,전태원,정태천,Yun, Soo-Hong,Kim, Duk-Hyun,Lee, Sang-Kyu,Kim, Ju-Hyun,Seo, Young-Min,Kang, Mi-Jeong,Jeon, Tae-Won,Jeon, Tae-Cheon 대한약학회 2007 약학회지 Vol.51 No.4

Hepatoprotective effects of the water extract of Protaetia brevitarsis larva (PB) was investigated in carbon tetrachloride ($CCI_{4}$) treated male Sprague-Dawley rats. PB administration protected rats against ALT, AST and LDH elevations induced by $CCI_{4}$, as well as the severity of liver damage. PB recovered the decrease in serum level of high-density lipoprotein-cholesterol and the increase in serum level of low-density lipoprotein-cholesterol induced by $CCI_{4}$. In histopathological observation, massive fatty change and necrosis in the centrilobular area, degenerative change including pyknosis of nucleus and swelling of parenchymal cell induced by $CCI_{4}$ were clearly protected by PB. These histopathological findings paralleled with the serum biochemical results. The present results demonstrated that the water extract of PB may have the hepatoprotective effect against $CCI_{4}$-induced liver damage in vivo.