Benzyl Isothiocyanate Inhibits Prostate Cancer Development in the Transgenic Adenocarcinoma Mouse Prostate (TRAMP) Model, Which Is Associated with the Induction of Cell Cycle G1 Arrest

Cho, Han Jin,Lim, Do Young,Kwon, Gyoo Taik,Kim, Ji Hee,Huang, Zunnan,Song, Hyerim,Oh, Yoon Sin,Kang, Young-Hee,Lee, Ki Won,Dong, Zigang,Park, Jung Han Yoon MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.2

<P>Benzyl isothiocyanate (BITC) is a hydrolysis product of glucotropaeolin, a compound found in cruciferous vegetables, and has been shown to have anti-tumor properties. In the present study, we investigated whether BITC inhibits the development of prostate cancer in the transgenic adenocarcinoma mouse prostate (TRAMP) mice. Five-week old, male TRAMP mice and their nontransgenic littermates were gavage-fed with 0, 5, or 10 mg/kg of BITC every day for 19 weeks. The weight of the genitourinary tract increased markedly in TRAMP mice and this increase was suppressed significantly by BITC feeding. H and E staining of the dorsolateral lobes of the prostate demonstrated that well-differentiated carcinoma (WDC) was a predominant feature in the TRAMP mice. The number of lobes with WDC was reduced by BITC feeding while that of lobes with prostatic intraepithelial neoplasia was increased. BITC feeding reduced the number of cells expressing Ki67 (a proliferation marker), cyclin A, cyclin D1, and cyclin-dependent kinase (CDK)2 in the prostatic tissue. <I>In vitro</I> cell culture results revealed that BITC decreased DNA synthesis, as well as CDK2 and CDK4 activity in TRAMP-C2 mouse prostate cancer cells. These results indicate that inhibition of cell cycle progression contributes to the inhibition of prostate cancer development in TRAMP mice treated with BITC.</P>

Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

치료 경험이 없는 만성 B형간염 환자에서 엔테카비어와 아데포비어의 24주간 치료효과에 대한 비교

한경선,윤기태,허정 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

생활쓰레기에 대한 아산시민 의식 조사 분석

한성현,박종안,손부순,이종화,장봉기,염윤기 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2

This study was designed to identify the recognition and practice for delivery method of living waste, to find out the problem of currently delivery system of living waste and to recommend the more effected waste delivery system in Asan city. The size of sample was 1100 households of 50000 households who live in Asan area. The data was collected by questionnaire from June 1999 to May 2000, and the results were as follows; 1. The 58% respondents(housewives) has been answered to know for recycling method of living waste, and 50% of the respondents has been discharge separately the food. 2. The respondents has been answered that the priority for effected method of living waste delivery system in to discharge separately the living waste, to reduce the consumption and to charge for vinyl package.

화상을 입힌 생쥐의 흉선과 비장 임파구아형과 조직학적 소견의 변화

한기택,안상태,강윤섭,임 풍 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.4

Due to various documented abnormalities of cell mediated immunity, sepsis still remains the major cause of morbidity and mortality after initial survival from extensive thermal burn. However, the exact mechanisms of immunosuppression after extensive thermal burn remains uncertain. For th observation of immune state, changes of lymphocyte subpopulation have been usually measured in lymphocyte samples from peripheral blood by many reports, but it is only a small fraction of the total lymphocyte pool in the body, and may not present valid picture of the immunological state of the individual. Therefore, analysis of lymphocyte phenotype in thymus and spleen is thought to be necessary in understanding more exact mechanism of immunological changes in extensive thermal burn. The aim of this study is to evaluate the changes of immunocompetency by observing the changes of subpopulation of spleen and thymus lymphocyte using monoclolnal antibodies and changes of tissue with light microscope in burned mice. Cell populations were isolated from spleen and thymus of control and burned mice on the 1st, 3rd, 5th, 7th, 14th and 21st postburn days(PBD). The cell populations were measured with monoclonal antibody by flow cytometry. The results were as follows: 1.In thymus, Thy 1.2+, L3T4+ and LYt2+ cells decreased markedly on the 3rd PBD. Thy 1.2+ and L3T4+ cells decreased also on 5th, 7th, 14th PBD and returned to control level on 21st PBD, but Lyt2+ cells increased more significantly than control after 3rd PBD and returned to normal on 21st PBD. Qa2+ cells revealed most significant reduction on 7th PBD. 2.In spleen, Thy 1.2+, L3T4+ and Ia+ cells increased on 3rd PBD and then decreased on 7th and 14th PBD. Lyt2+ cells increased in all the burned groups. Qa2+ cells increased on 3rd, 5th PBD and returned to normal level. L3T4+ cell/Lyt2+ cell ratio revealed signicant reduction, but they recovered by the 21st PBD. 3.In histopathologic findings of the spleen & thymus, the all cell subpoulations decreased at 1st PBD, but more increased than that of group of 3rd PBD. And then they revealed normal architecture after 5th PBD.

액상소결법에 의한 SiC 세라믹스의 파괴인성 평가

윤한기,정헌채,김부안 동의대학교 산업기술개발연구소 2005 産業技術硏究誌 Vol.19 No.-

Monolithic Liquid Phase Sintered SiC (LPS-SiC) was fabricated by hot pressing method under 20 MPa using sintering additives at different temperature such as 1760 ℃, 1780 ℃, 1800 ℃ and 1820 ℃. The starting powder was high purity 13-SiC nano-powder with an average particle size of 30 nm. Compositionsof sintering additives were Al_(2)O_(3) Y_(2)O_(3) = 60.7 and 1.5 (wt%). Monolithic LPS-SiC was evaluated in terms of sintering density, hardness and fracture toughness through indentation fracture method by the Vickers hardness tester. Sintered density, hardness and fracture toughness of fabricated LPS-SiC increased with increasing the sintering temperature. It is higher value than that of fabricated SiC by the chemical vapor deposition method.

FEM에 의한 Al07075/CFRP 적층재의 탄성계수 해석

윤한기,안광주,김연겸,박준수 東義大學校産業技術開發硏究所 1998 産業技術硏究誌 Vol.12 No.-

As for the properties on both the aluminum and the CFRP which are used to make A17075/CERP multi-layered hybrid composites, CRALL(carbon reinforced aluminum laminate). For CRALL specimen, FEM analysis is compared with experimental results. It is shown that the rule of mixture is well agreed with experiment result in A/C 0001 layered 1 ply. However, differences are conceivable in A/C 0003 and A/C and A/C 0005 because of the effect of fiber volume fraction. In the A/C 0001 specimen case, the elastic modulus of the unnotched and circular notched CRALL specimen by FEM analysis are agreed with the experimental results, but that of A/C 0003 and A/C 0005 specimens had high CFRP volume fraction doesn't agree with experimental results.

외상성 신동맥 색전증 1례

한병규,김욱현,문기혁,송윤섭,박영호 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Renal artery thrombosis may result from external trauma of a blunt nature. Such injury is induced by rapid deceleration resulting in the stretching of the renal vasculature, disruption of the arterial intima-the least elastic layer of the arterial wall and eventual thrombosis. The treatment of renal artery thrombosis is controversial, with physician's options divided between prompt surgical exploration with vascular repair and clinical observation. We experienced 1 case of traumatic renal artery thrombosis and report that with a brief review of the literature.

국내기업의 팀제 도입에 따른 효율적 운영방안에 관한 연구

한기수,윤광심 대불대학교 1999 論文集 Vol.5 No.1

In this study, team organizational effectiveness and effectiveness and efficiency of team was analyzed and how to grade it in company K was suggested. First, definition, purpose, precondition and management method of teams organization was explained, and then theatrical consideration was made on putting the team organization management into practices. Second, problems was explained based on that analysis. Third, methods were suggested how to cope with the situation. Major finding and some suggestion of the study in the field of management of team in company K as follws : Teams develop direction, momentum, and commitment by working to shape a meaningful purpose. Management is responsible for clarifying the charter, retionale, and leave enough flexibility for the term to develop commitment around its own spin on that purpose, set of specific goals, timing, and approach. Effective teams develop strong commitment to a common approach, that is to how they will work together to accomplish their purpose. team members must agree on how will do particular jobs, how schedules will be set and adhered to, what skills need to be developed, how continuing membership in the team is to be earned, and how the group will make and modify decisions.