Fluoxetine and Sertraline Attenuate Postischemic Brain Injury in Mice

Shin, Tae-Kyeong,Kang, Mi-Sun,Lee, Ho-Youn,Seo, Moo-Sang,Kim, Si-Geun,Kim, Chi-Dae,Lee, Won-Suk The Korean Society of Pharmacology 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.3

This study aimed to investigate whether selective serotonin reuptake inhibitors (SSRIs) attenuate brain injury and facilitate recovery following photothrombotic cortical ischemia in mice. Male ICR mice were anesthetized and systemically administered Rose Bengal. Permanent focal ischemia was induced in the medial frontal and somatosensory cortices by irradiating the skull with cold light laser. The animals were treated with fluoxetine or sertraline once a day for 14 d starting 1 h after ischemic insult. Treatment with fluoxetine and sertraline significantly reduced the infarct size. The Evans blue extravasation indices of the fluoxetine- and sertraline-treated groups were significantly lower than that of the vehicle group. Treatment with fluoxetine and sertraline shifted the lower limit of the mean arterial blood pressure for cerebral blood flow autoregulation toward normal, and significantly increased the expression of heme oxygenase-1 (HO-1) and hypoxia-inducible factor-1 ${\alpha}$ (HIF-1 ${\alpha}$) proteins in the ischemic region. These results suggest that SSRIs, such as fluoxetine and sertraline, facilitate recovery following photothrombotic cortical ischemia via enhancement of HO-1 and HIF-1 ${\alpha}$ proteins expression, thereby providing a benefit in therapy of cerebral ischemia.

Fluoxetine and Sertraline Attenuate Postischemic Brain Injury in Mice

Tae Kyeong Shin,Mi Sun Kang,Ho Youn Lee,Moo Sang Seo,Si Geun Kim,Chi Dae Kim,Won Suk Lee 대한생리학회-대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.3

This study aimed to investigate whether selective serotonin reuptake inhibitors (SSRIs) attenuate brain injury and facilitate recovery following photothrombotic cortical ischemia in mice. Male ICR mice were anesthetized and systemically administered Rose Bengal. Permanent focal ischemia was induced in the medial frontal and somatosensory cortices by irradiating the skull with cold light laser. The animals were treated with fluoxetine or sertraline once a day for 14 d starting 1 h after ischemic insult. Treatment with fluoxetine and sertraline significantly reduced the infarct size. The Evans blue extravasation indices of the fluoxetine- and sertraline-treated groups were significantly lower than that of the vehicle group. Treatment with fluoxetine and sertraline shifted the lower limit of the mean arterial blood pressure for cerebral blood flow autoregulation toward normal, and significantly increased the expression of heme oxygenase-1 (HO-1) and hypoxia-inducible factor-1Ձ (HIF-1Ձ) proteins in the ischemic region. These results suggest that SSRIs, such as fluoxetine and sertraline, facilitate recovery following photothrombotic cortical ischemia via enhancement of HO-1 and HIF-1Ձ proteins expression, thereby providing a benefit in therapy of cerebral ischemia.

최적화에 의한 Ketoconazole Solid Dispersion System 의 약물 속도론적 연구

신영희,박중남,이치호 慶星大學校 1991 論文集 Vol.12 No.3

Ketoconazole was manufactured as solid dispersion system(SDS)by computer optimization technique and pharmacokientic properties were investigated in vivo by animal study using rabbits. Following results, doses (25,50 and 100mg/kg)and AUC?? had both a good relationship between them administered by SDS as well as ketoconazole only. AUC?? obtained by administration of ketoconazole only, physical mixture and SDS was occurred SDS>physical mixture>ketoconazole only in turn and plasma comcentration was increased about 2.3 fold by SDS administration than ketoconazole only. The results obtained by in vivo absorption test of SDS that made maximum dissolution in the artificial intestinal juice revealed a good relationship between in vitro and in vivo test.

지연성 방사선 뇌괴사 -1예 보고-

강신광,송준호,이규호,주인욱,지제근 대한병리학회 1988 Journal of Pathology and Translational Medicine Vol.22 No.3

씀바퀴 성분 중 Cynaroside의 약동태학적 연구

신영희,민병훈,이치호 慶星大學校 1998 論文集 Vol.19 No.1

We isolated cynaroside(luteolin-7-O-glucoside) from the whole plant of Ixeris dentata, and characterized pharmacokinetic properties of cynaroside. It was administered by intravenous injection, intraperitoneal injection and oral routes to male rats, and plasma concentrations of cynaroside were analysed by HPLC method. After iv injection of cynaroside with 5mg, 10mg, and 20 mg per kg of body weight, the area under concentration curve and doses showed a good correlationships. On the other hand, cynaroside hardly absorbed by oral administration route.

혈액투석중인 만성신부전 환자에서 골대사 지표로써의 Osteocalcin치

송치운,이진홍,안미애,윤환중,윤상임,성기양,이강현,송민호,이강욱,신영태,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Background : Serum osteocalcin is synthesized by osteoblast and has been shown to be sensitive indicator of bone turnover inpatients with various metabolic bone disease. In renal osteodystrophy, serum osteocalcin is elevated due to decreased renal clearance and elevated level of PTH. This study was done to evaluate the usefulness of serum osteocalcin as a marker of bone metabolism and the correlation with other biochemical markers of bone metabolism. Methods : We measured serum osteocalcin, calcium, phosphorus, ALP(alkaline phosphatase) and PTH(parathyroid hormone) in 37 patients with end stage renal disease on hemodialysis. Osteocalcin was determined by radioimmunoassay and PTH was determined by radioimmunometric assay. Results : 1) The mean level of serum osteocalcin in ESRD patients was 233.8± 218.2ng/ml which was significantly higher than that of controls(p<0.0001). 2) The mean level of serum PTH in ESRD patients was 40.5± 43.8pg/ml was significantly higher than that of controls(p<0.005). 3) There was a significant positive correlation between the level of serum PTH, ALP and the level of serum osteocalcin in ESRD patients. 4) By using multiple regression, PTH is most reliable factor that affect to elevated level of serum osteocalcin ( beta coefficient = 0.687, Sig T<0.05). Conclusion : Serum osteocalcin as a marker of bone metabolism in ESRD patients is more useful than other biochemical marker such as serum calcium, phosphorus, ALP and PTH is a most reliable factor that affect to elevated level of serum osteocalin.

자연수계내에서 환경호르몬 Bisphenol-A의 생분해평가

강호,신경숙,김치열,이준재 忠南大學校 環境問題硏究所 2002 環境硏究 Vol.20 No.-

본 연구에서는 환경호르몬인 Bisphenol-A(BPA)가 하천 및 호소내에 유입되었을 경우 어떠한 분해패턴을 나타내는지 고찰하기 위하여 시뮬레이션 생분해시험법인 TOC-HANDAT을 이용하여 BPA의 자연 수계내에서의 생분해율과 생분해속도를 평가하였다. 금강수계를 대상으로 하여 유구천, 논산천, 초강천, 갑천등 4개 하천과 대청호수 및 대청댐유역의 호소수로부터 미생물원을 채취하고 이를 BPA생분해평가를 위한 식종원으로 사용하였다. 생분해시험환경의 적합성을 평가하기 위하여 표준물질로 사용한 Phenol과 Aniline이 적응기없이 곧바로 분해되어 최종적으로 88%의 높은 생분해율을 나타낸 것과는 달리, BPA는 상대적으로 미생물수가 많고 오염도가 타 하천에 비해 심한 갑천의 경우를 제외하고는 대부분의 조사대상 하천에서 초기 5일이내의 적응기를 갖으며, 이후 활발한 분해를 보이다가 더이상 분해가 되지 않는 생분해패턴을 나타내었으며, 이러한 경향은 각 하천의 상류지점에서 보다 뚜렷하였다. 또한 호소의 경우 하천과 인접해 있는 지점에서는 하천과 유사한 분해 패턴을 보인 반면, 대청댐지점에서는 BPA분해율이 28%에 그치는 것으로 나타나 자연수계내의 미생물군의 존재형태가 BPA의 분해에 있어 매우 중요한 것으로 나타났다. 또한 시간경과에 따른 BPA의 생분해속도상수를 세분하여 산정한 결과 분해후반부의 생분해속도가 매우 저조한 친으로 나타나 BPA의 분해로 생성된 2차 대사산물이 매우 난분해성 물질임을 알 수 있었다. The TOC-HANDAI method was utilized to examine the degree of biodegradation of endocrine disruptor, bisphenol-A(BPA), in terms of TOC degradation within 28 days using natural water microcosm. Anilind and phenol, as standard chemicals, were easily mineralized without any adaption periods and showed the relatively high biodegradability of 88% BPA were mineralized 63~85% of initial TOC concentration by natural microcosm from each sampling stream except Gap Stream, whereas the biodegradability of BPA by the microcosm in Dadchung Reservoir was only 28%. This ws probably due to the numbers and the activity of participated microorganisms during the degaradation of BPA. BPA degradation described by three distinct first order decay retes(k_1, k_2 and k_3) which could be separated by visual fitting. The k_1 represented the initial lag phase and the k_2 showed the vigorous degradation of BPA and the following another long term first order decay rate coefficient, k_3, showed much flat slop. The remaining the 15~37% of initial TOC at the end of experiment considered to be refractory methabolites of BPA.

Monocyte Chemoattractant Protein-1[MCP1] -2518 유전자 다형성과 주요 우울장애

배치운,이지현,신윤경,김태석,김정진,이창욱,이수정,전태연,이철,백인호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Object : This study was designed to examine the association between monocyte chemoattractant protein-l (MCPl) -2518poly morphism and major depressive disorder (MDD). Methods : Ninety patients with MDD and 114 healthy controls participated in this study. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism method. Results : Genotype and allele distributions in patients with MDD were significantly different from those of the controls, In particular, subjects with the allele A were found to have an increased risk of MDD. Conclusion : The present study suggests that the MCPl -2518 polymorphism may have a potential role for susceptibility to MDD in the Korean population and thus calls for consecutive studies in order to pile up the data with larger different ethnic background.

고립성 폐결절의 조직학적 진단에 있어서 경기관지폐생검의 진단적 가치

김치훈,김정주,왕준호,연규민,김형수,리원연,용석중,신계철 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

Transbronchial lung biopsy(TBLB) is a relatively simple and convenient diagnostic tool in solitary pulmonary nodule(SPN) on chest radiograph, which is cannot be diagnosed through routine tests. To investigate the diagnostic value and diagnostic rate of TBLB according to the variable factor, we analyzed 69 patients with radiologically SPN lesion on chest radiograph which was confirmed histologically. A histologic diagnosis of TBLB was made in 47 of 69 patients(68.1%), in which 26 of 37(70.3%) with malignancy and 21 of 32(65.6%) with benign lesion. There were no significant differences in diagnostic yield according to age, sex and location of the lesion. The diagnostic rate tended to increase with the more than 5 pieces to acquired by TBLB. When the size of SPN was less than 3 ㎝, it cannot be diagnosed. In conclusion, TBLB is a diagnostic procedure with good yield and safety, but percutaneous needle aspiration, transbronchial needle aspiration, and bronchial washing fluid cytology may be a complementary procedure to overcome the limitation of TBLB and get higher diagnostic yield for SPN.

초·중·고 학생들의 과학 태도 변화에 대한 학습환경의 원인 분석

권치순,허명,양일호,김영신 한국과학교육학회 2004 한국과학교육학회지 Vol.24 No.6

최근 학교교육에서 학생들의 과학 태도에 대한 관심과 중요성이 증대되고 있다. 학생들의 과학 태도는 학업 성취도, 교육 프로그램의 일관성, 수업의 질뿐만 아니라 과학 교육의 관점과 과학 관련 직업 선택에도 영향을 준다. 그 동안의 과학 태도에 대한 선행 연구와 국제 비교 연구에 의하면, 우리나라 학생들의 과학 태도는 점차 하락하고 있는 것으로 나타났다. 이 연구에서는 초ㆍ중ㆍ고 학생들의 과학 태도 변화 과정을 알아보고, 이에 영향을 미치는 학습 환경 변인을 밝히는데 그 목적이 있다. 초등학교 3학년부터 고등학교 2학년 학생 약 6,925명을 대상으로 2003년 3월부터 3회에 걸쳐 학생들의 과학 태도와 학습 환경 검사를 실시하였다. 분석 결과, 학생들의 과학 태도는 일년 중 6월 이후에 낮아지고, 초등학교 4학년에서 중학교 2학년까지 과학 태도가 크게 변하였다. 과학 태도가 1학기보다는 2학기에 더 많이 하락하였으며, 초?중학교 학생들보다 고등학생들의 과학 태도 하락 폭이 더 컸다. 학생들의 과학 태도에 가장 큰 영향을 주는 요인은 과학에 대한 경험이고, 교사, 학급 분위기 등이 과학 태도 변화에 영향을 주는 것으로 나타났다. 학습 환경 변인은 과학 태도가 하락한 학생과 과학 태도가 상승한 학생이 각각 다르게 영향을 주는 것으로 밝혀졌다. 과학 태도에 영향을 주는 범주는 초등학교에서는 성, 학년, 지역, 중학교에서는 학년과 지역, 그리고 고등학교에서는 지역으로 나타났다. The importance of science attitudes is more increasing in science education. Science attitudes may influence students' attainment, consistency and quality of classwork as well as their later views of science education and scientific occupations. According to the international comparative researches and longitudinal studies on Korean students' science attitudes, it has shown that the more grade, the less science attitude. This research was survey the science attitudes and learning environment variables, and then make a inquiry that causes of decline of science attitudes. To study this purpose, the participating students in this study will be selected from 3th to 11th grade. 6,925 participants were administered 3 times in questionnaires of science attitudes and learning environment variables during a year. The result of this study showed that science attitude got low after June. Science attitude was changed from 4th grade to 8th grade students. Science attitude much more decrease second semester than first semester, high school students' science attitude fell much. It was experience about science that cause the biggest effect in science attitude and other learning environment variables influence in science attitude change. Learning environment variables made different influence from students of increased and declined science attitude. As category that influence in science attitude, in elementary school were gender, area and grade, in middle school were grade and area, and in high school was area.