Enhanced Densification and Hardness of Titanium Bodies Sintered by Advanced Hydrogen Sintering Process

Jung‑Min Oh,Ja‑Geon Koo,Jae‑Won Lim 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.6

A new sintering technique for enhancing a densification and hardness of sintered titanium body by supplying hydrogen wasdeveloped (Hydrogen Sintering Process, HSP). The HSP was developed by only injecting hydrogen into an argon atmosphereduring the core time. As a result, sound titanium sintered bodies with high density and hardness were obtained by the HSP. In addition, a pore size and number of the HSP specimens were smaller than those of the argon atmosphere specimen. It wasfound that the injecting hydrogen into the argon atmosphere by HSP can prevent the formation of oxide layers, resulting inenhanced densification and hardness.

주요우울증에 대한 벤라팍신의 효과 및 안전성

이민수,남종원,강성민,연병길,오병훈,이철,정인과,채정민,백인호 대한신경정신의학회 2000 신경정신의학 Vol.39 No.6

연구목적 : 주요우울증 환자에서 벤라팍신의 치료 효과와 안정성을 조사하기 위해 다기관 개방연구를 시행하였다. 방 법 : DSM-IV 진단 기준에 의거하여 주요우울증으로 진단된 환자중 벤라팍신 투여에 동의한 환자를 대상으로 하였다. 선택된 환자에서 다른 항우울제를 복용하고 있는 경우 14일 이상의 약물 배설기간을 가진후 벤라팍신을 6주간 투여하였으며, 기준점, 1주, 2주, 4주, 6주 후에 HAM-D, MADRS, 그리고 CGI등을 사용하여 평가하였다. 본 연구기간중 발생된 모든 부작용은 부작용의 발생 및 소설 시기, 심한 정도, 발생 빈도, 벤라팍신과의 관련성, 관련 조치 및 결과에 대하여 증례기록서에 기록하도록 하였다. 결 과 : 총 141명을 대상으로 하였으며, 이중 94명(66.7%)이 6주간의 연구를 완결하였고, 47명(33.3%)이 중도탈락하였다. HAM-D총점수는 벤라팍신 투여 1주후부터 유의한 감소를 나타내었으며, 2주,4주, 6주후에도 지속적인 감소를 보였다. MADRS의 총점수도벤라팍신 투여 1주 후부터 유의한 호전을 나타내었으며, 2주, 4주, 6주 후에도 지속적인 감소를 보였다. CGI에서도 기준점, 1주, 2주, 4주, 6주 후 각각의 시점 사이에 유의한 호전이 보였다. 활력징후, 일반 혈액학, 생화학, 심전도 검사 등에서 유의한 변화는 없었다. 벤라팍신과 관련된 부작용으로 흔히 보고한 증상은 오심(10.6%), 소화불량(9.5%), 변비(8.5%), 현기증(8.5%)등 이었다. 결 론 : 다기관 개방연구를 통해 주요우울증 환자에 대한 벤라팍신의 투여는 우울증상의 호전에 효과적이었으며 안전하였다. Objective : A Multicenter open-label study was conducted to evaluate the clinical efficacy and safety of venlafaxine for the tretment in patients with major depression. Method : The study was done in patients with major depression diagnosed by DSM-IV who accepted venlafaxine medication. In cases of the patients taking other antidepressants, 6 weeks of venlafaxine medication was carried out after 14 days of drug excretion period and evaluation using HAM-D, MADRS, and CGI was done at baseline, and after 1, 2, 4, and 6 weeks. Regarding all side effects that had occurred during the period of our study such as their developed/disappeared time, severities, incidences, relationship with venlafaxine, managements and results have been putted into the records. Results : A total of 141 patients were enrolled. Among 94 of them finished the 6 weeks of research and 41 of them did not make it through the research. Total HAM-D scores showed significant decrease after 1 week of venlafzxine medication and continous decrease through the study period. Total scores of MADRS also showed significant improvement after 1 week and continuous decrease through the study period. Similarly, CGI showed significant improvement between baseline, 1, 2, 4, and 6 weeks. There were no significant changes in vital sign, CBC, chemistry, and EKG. The commonly reported side effects of venlafaxine were nausea(10.6%), indigestion(9.5%), constipation(8.5%), and dizziness(8.5%). Conclusion : According to the results, venlafaxine was effective and safe in the treatment of patients with major depression.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

대황의 항산화 효과 및 타이로시네이즈 저해 활성

김정은 ( Jung Eun Kim ),박찬하 ( Chan Ha Park ),오대석 ( Dae Seok Oh ),이승연 ( Seung Yeon Lee ),장세훈 ( Se Hun Jang ),홍지연 ( Jee Yeon Hong ),민혜진 ( Hye Jin Min ),박수아 ( Su Ah Park ),원두현 ( Doo Hyun Won ),박수남 ( Soo Nam 대한화장품학회 2011 대한화장품학회지 Vol.37 No.4

본 연구에서는 대황 추출물의 항산화 활성, 타이로시네이즈(tyrosinase) 저해 활성을 확인하였다. 대황의 50 % 에탄올 추출물, 에틸아세테이트(ethyl acetate) 분획, 아글리콘(aglycone) 분획으로 실험을 진행하였다. 대황 추출물들의 DPPH (1,1-diphenyl-2-picrylhydrazyl) 소거활성(FSC₅₀)은 대표적인 항산화제인 (+)-α-tocopherol보다 낮은 것으로 나타났다. Luminol 발광법을 이용한 Fe³⁺-EDTA/H₂O₂ 계에서 생성된 활성산소종에 대한 아글리콘 분획의 소거활성(총 항산화능, OSC₅₀)은 0.265 μg/mL로 매우 큰 활성을 나타내었다. 대황 추출물의 rose-bengal로 증감된 ¹O₂에 의한 적혈구 파괴에 대한 세포보호 효과는 모든 분획에서 농도 의존적(1∼50 μg/mL)으로 증가하였으며, 특히 아글리콘 분획은 10 μg/mL 농도에서 τ₅₀이 757.0 min으로 높은 세포 보호 활성을 나타내었다. 대황 추출물 중 아글리콘 분획의 타이로시네이즈 저해활성(IC₅₀)은 11.20 μg/mL으로 226.88 μg/mL인 알부틴(arbutin)보다 큰 활성을 보여주었다. 이상의 결과들로부터 대황 추출물은 활성산소종을 소거하는 항산화제로 이용가능하며, 특히 아글리콘 분획의 현저한 항산화 작용 및 큰 타이로시네이즈 저해 효과로부터 이들 분획 또한 화장품원료로서 응용 가능성이 큼을 알 수 있었다. In this study, the antioxidative effects and inhibitory activities on tyrosinase of Rheum undulatum (R. undulatum) L. extracts were investigated. 50 % ethanol extract, ethyl acetate and aglycone fractions of R. undulatum L. were used in experiments. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities (FSC₅₀) of R. undulatum L. extracts was lower than (+)-α-tocopherol, known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) of aglycone fraction on ROS generated in Fe³⁺-EDTA/H₂O₂ system using the luminol-dependent chemiluminescence assay showed the most prominent effect at a concentration of 0.265 μg/mL. The cellular protective effects of extract/fractions of R. undulatum L. on the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner (1 ~ 50 μg/mL). Especially, aglycone fraction in 10 μg/mL concentration showed the most protective effect among extracts (τ₅₀, 757.0 min). The inhibitory effects (IC₅₀, 11.20 μg/mL) on tyrosinase of aglycone fraction was much higher than arbutin (226.88 μg/mL), known as a whitening agent. These results indicate that R. undulatum L. extracts can be used as antioxidant. Particularly, aglycone fraction of R. undulatum L. showed superior antioxdative activity and high inhibitory effect on tyrosinase. Therefore, aglycone fraction of R. undulatum L. could be applicable to new functional cosmetics.

The Korean Association of lnternal Medicine : Poster Session ; PS 1357 : Nephrology ; Age is the Strongest Effector for the Relationship between Estimated Glomerular filtration Rate and Coronary Artery Calcifi cation in Apparently Healthy Korean Adults

( Hyun Beom Chae ),( Eun Jung Rhee ),( Hae Jung Park ),( Min Kyung Lee ),( Won Seon Jeon ),( Se Eun Park ),( Cheol Young Park ),( Won Young Lee ),( Ki Won Oh ),( Sung Woo Park ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Cardiovascular disease (CVD) is a major clinical problem and the most common cause of morbidity and mortality in patients with chronic kidney disease (CKD). Coronary artery calcifi cation (CAC) is a potential mechanism that explains the association between renal function and cardiovascular mortality. We aimed to evaluate the association between renal function and CAC in apparently healthy Korean subjects. Methods: In 23,617 participants in the health screening program in Kangbuk Samsung Hospital health screening program from January 2010 to December 2011, estimated glomerular fi ltration rate (eGFR) was assessed by Cockcroft-Gault equation. Coronary artery calcium score (CACS) was measured by multi-detector computed tomography (MDCT). Subjects were divided into 3 groups according to the CKD Staging system with eGFR grade; stage 1: eGFR = 90 mL/min/1.73 m2, stage 2: eGFR 60-89 mL/ min/1.73 m2, stage 3: eGFR 30-59 mL/min/1.73 m2. Results: Mean age of the participants was 41.4 years, and mean eGFR was 103.6±21.7 mL/min/1.73 m2. Hypertension and diabetes were noted in 43.7% and 5.5% of the participants, respectively. eGFR showed weakly negative but signifi cant association with CACS in bivariate correlation analysis (r=-0.076, p<0.01). Mean CACS signifi cantly increased from CKD stage 1 to 3. The proportion of subjects who had CAC signifi cantly increased from CKD stage 1 to 3. Although odds ratio for CAC signifi cantly increased from stage 1 to 3 after adjustment for confounding factors, this signifi cance was inversed when age was included in the model. Age was the strongest factor that affects CAC. Conclusions: This study performed in young Korean subjects with relatively normal renal function, age was the mostly strongest effector on the association between eGFR and the degree of CAC.

위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동

이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.

정조(正祖) 초장지(初葬地) 출토 옥의 과학적 분석 및 보존처리

이태종,오정현,김사덕,이정민,Lee, Tae-jong,Oh, Jung-hyeon,Kim, Sa-dug,Lee, Jung-min 국립문화재연구원 2013 헤리티지:역사와 과학 Vol.46 No.4

This research aims to understand the precise character of relics based on literature search and material analysis of the jade excavated from the first buried place of King Jung-jo, and to assess the level of damage through non-destructive diagnosis. Furthermore, scientific conservation treatment was used to restore the original shape of the excavated jade. According to literature search, the excavated jade is known to be jasper, but material analysis showed that it was as a serpentine with the mineral composition of antigorite. Infrared thermography analysis to assess deterioration showed that the internal damage is the result of the interstices developed along the boundary surface of the obtained jade. For conservation treatment of the damaged area on the surface, the jade was filled with a mixture of plaster and glue, and covered with a mixture of acrylic paint and gloss medium for protection, and color was adjusted. 본 연구는 정조 초장지에서 출토된 옥에 대한 문헌조사와 재질분석을 통해 유물의 재질 특성을 밝히고 비파괴진단을 통해 출토 옥의 손상도를 평가하였다. 또한 과학적 보존처리를 실시하여 출토 옥의 원형을 복원하였다. 문헌조사 결과 출토 옥은 벽옥으로 알려졌으나 재질분석 결과, 출토 옥은 안티고라이트(Antigorite) 광물조성의 사문암으로 판단된다. 열화상분석 등 손상도 평가 결과 내부손상 부분은 포획물의 경계면을 따라 발달한 간극에 의한 것으로 나타난다. 표면손상 부분의 보존처리는 석고와 아교를 혼합하여 충전하였으며 보존처리용 아크릴물감과 그로스미디엄을 혼합한 후 색맞춤하였다.

HSS 기반 초고속 LDPC 복호를 위한 구조

이인기(In-Ki Lee),김민혁(Min-Hyuk Kim),오덕길(Deock-Gil Oh),정지원(Ji-Won Jung) 한국통신학회 2013 韓國通信學會論文誌 Vol.38 No.2B

본 논문은 위성방송 전송 규격인 DVB-S2의 기반 채널 부호로 사용되는 LDPC를 고속 복호를 위해 HSS(Horizontal Shuffle Scheduling) 방식을 기반으로 고속 복호기 구조를 연구하였다. 첫째로 HSS방식에서 발생하는 메모리 충돌을 극복하는 방식을 제시한다. 둘째로 고속 복호를 위해 LUT(Look Up Table)을 이용하는 Sum-Product 알로리즘 대신 min값에서 scaling factor를 곱하는 Normalized Min-Sum 알고리즘을 사용하였으며, 성능 향상을 위해 check node에서 bit node로 입력되는 값의 부호를 확인하여 신뢰성 없는 값을 삭제하는 Self-Correct 방식을 제시하여 sum-product 방식에서 발생하는 bottle neck 현상을 하였다. 마지막으로 고속화를 위한 효율적인 메모리 구조를 제안한다. This paper proposes the high speed LDPC decoder structure base on the DVB-S2. Firstly, We study the solution to avoid the memory conflict. For the high speed decoding process the decoder adapts the HSS(Horizontal Shuffle Scheduling) scheme. Secondly, for the high speed decoding algorithm normalized Min-Sum algorithm is adapted instead of Sum-Product algorithm. And the self corrected is a variant of the LDPC decoding that sets the reliability of a Mc→v message to 0 if there is an inconsistency between the signs of the current incoming messages Mv’→c and the sign of the previous incoming messages Moldv’→c This self-corrected algorithm avoids the propagation on unreliable information in the Tanner graph and thus, helps the convergence of the decoder.Start after striking space key 2 times. Lastly, and this paper propose the optimal hardware architecture supporting the high speed throughput.

간헐식 흐름방식을 활용한 수직 · 수평 습지의 정화효율 평가

주광진(Kwang Jin Joo),이동민(Dong Min Lee),김기중(Ki Jung Kim),조용철(Yong Chul Cho),장광현(Gwang Hyeon Jang),최이송(I Song Choi),오종민(Jong Min Oh) 응용생태공학회 2017 Ecology and resilient infrastructure Vol.4 No.3

본 연구에서는 부영양화의 원인이 되는 질소와 인의 제거 효율을 향상시킬 목적으로 광물질을 활용한 여재를 이용하여 호기, 혐기의 흐름조건을 만들어주기 위한 수직·수평 흐름 인공습지를 고안하여 아크릴 반응조로 제작한 후 성능평가를 진행하였다. 수직·수평 흐름형 인공습지의 경우 호기 및 혐기조건을 평가하기 위해서 반응조 내 용존산소(DO) 농도를 측정해본 결과 호기상태에서는 2.7 ㎎/L, 혐기상태에서는 N.D로 확인되어 목적에 부합된 결과가 확인되었다. 실험결과에서는 SS 저감효과가 140 min, 80 min, 60 min의 운전시간에서 각각 94%, 91%, 61%의 효율을 보였고, T-P의 경우 각각의 운전시간에 따라 84%, 71%, 63%의 저감효율을 보였다. 또한 T-N의 경우 각각의 운전시간에 따라 63%, 49%, 42%의 저감효율을 보여 기존의 습지가 12~24 hr 체류시간을 가지는 것에 비하여 짧은 운전시간에도 높은 처리효율을 보이는 것으로 확인되었다. 본 연구에서는 수직·수평 흐름 방식을 복합 적용하여 기존 인공습지의 단점을 보완하기 위해 기술개발을 진행한 것으로 어떠한 기능적 효과를 갖는지 확인하였고, 향후 이에 대한 현장적용을 위한 운영 및 관리적 차원의 메커니즘 연구가 추가로 진행 될 필요가 있다. Nitrogen and phosphorus are key factors in causing eutrophication of water body. In this study, ceramics media was selected to increase the removal efficiency of nitrogen and phosphorus. We designed vertical, horizontal flow constructed wetlands to create aerobic and anaerobic flow conditions by using the media, then proceeded to performance evaluations after acrylic reactors were produced. In the case of vertical and horizontal flow constructed wetlands, we measured oxygen concentrations to evaluate aerobic and anaerobic conditions. we got the result of 2.7 ㎎/L in the aerobic condition, N.D in the anaerobic condition respectively, which suited our purpose. The result of the combined vertical and horizontal flow condition showed that the removal efficiency of SS was 94%, 91%, 61% at 140 min, 80 min, 60 min of running times, respectively, and the removal efficiency of T-P was 84%, 71%, 63% during each running time. In case of T-N, the removal efficiency was 63%, 49%, 42% during each running time. We found that the reactor exerted better removal efficiency when in the short time compared to 12 - 24 hr residence time of existing wetlands. In this study, we conducted experiments to explore functional effects after applying combined vertical and horizontal flow methods in the field. Further study will be carried out to identify its mechanism and administrative perspective.

개의 췌도분리에서 췌장의 팽창과 콜라겐 분해요소

이상훈,김성주,박정환,김종성,오승훈,한진수,정인경,양태영,김동준,김광원,이문규,민용기,이명식,정재훈,함종렬 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.2

Backgrounds: One of the main problems conditioning the outcome of islet transplantation is the ability to separate a sufficient number of viable islets with preserved function. Islet purification is critically affected by all of the isolation stages. Thus, it is necessary to set up the standard isolation method that islets are separate well from acinar without compromising islet yield and viability. Methods: Twenty three adult mongrel dogs were used for the experiment of total pancreatectomy with islet isolation. The islets were properly isolated by a modified Recordi method. The obtained islets were further purified by centrifugation on discontinuous gradients using cell separation system (Model 2991, Cobe, Lakewood Colo). We evaluated islet number (islet equivalent number, 150 um equivalents/kg of recipient body weight, IEq/kg), purity, cell volume, viabilty, recovery rate, and comparison of outcome according to the isolation conditions. Results: 1) The mean of islet numbers before purification were 13543±9431Eq/kg, digestion times were 13.8±2.6 min., digestion temperature was 37.4±0.2℃, purity was 59.7?.0%, viability was 90.0±2.1%, cell volume was 4.7±1.1 mL, islet number after purification were 4064±361 IEq/kg, and recovery rate was 29±2.9%. 2) Isolated islet numbers were different according to the degree of pancreas distension with collagenase, digestion temperature, and digestion time. 3) The best conditions for islet isolation were above 37.5℃ in temperature at recirculation of collagenase, within 12 min in digestion time and well distended pancreas with collagenase. 4) According to multiple regression adjusted by variable factors, the degree of pancreas distension with collagenase and digestion time were independently associated factors for successful islet isolation. Conclusions: In this study, we concluded that the degree of pancreas distension with collagenase and digestion time were independent factors for successful islet isolation and the best conditions for islet isolation were above 37.5℃ in temperature at recirculation of collagenase, within 12 min in digestion time and well distended pancreas with collagenase.