다양한 조건에서의 줄기세포 분리 수율 비교

정희연,김경은,김진명,이경석,김완종 순천향대학교 기초과학연구소 2020 순천향자연과학연구 논문집 Vol.26 No.1

Mesenchymal stem cells were isolated from adipose tissue in rat to determine optimized conditions. Adipose tissue was removed from dorsal wall of anesthetized Sprague-Dawley rat. We confirmed the stem cells as a shape of spindle with light microscopic finding during cell culture. The differences in the treatment time 30 min and 60 min, respectively) and in the volume (10 ml, 20ml and 30ml, respectively) of enzymes (trypsin or collagenase) solution resulted in establishing the optimal conditions for stem cell extraction. Results have shown that single treatment of trypsin is 3.2 times higher than that of collagenase. In addition, it was confirmed that extraction yield is most efficient when treated at 30 ml for 30 min in single treatment of trypsin.

우울증의 신경내분비적 고찰

정희연 순천향대학교 1989 논문집 Vol.12 No.1

I have previously noted that its possible involvement in depression is intriguing in light of the following four sets of findings taken from the disciplines of developmental psychology, clinical psychiatry, and neurophysiology: (a) Laboratory animals subjected to maternal deprivation during the neonatal period show significant hyperactivity of the HPA axis during stress throughout adult life Hence, such animals presumabley show a permanent change in the responsivity of their CRF neurons. (b) Clinical experience suggests that a history of early deprivation produces a diathesis to depression and a tendency to relive the intense anxiety and dysphoria associated with this early deprivation throughout adult life whenever a significant frustration or important loss occurs. Thus, such individuals also seem prone to a hyperresponisvity of their CRH neurons intermittently throughout of life. (c) CRH given ICV to experimental animals not only stimulates the HPA axis but also activates the locus ceruleus, produces decreased eating and sexual behavior, and causes significant changes in activity. (d) CRH has been reported to induce limbic seizures that cross sensitize with electrically kindled seizures. These findings, taken together, suggest that a CRH model of depression could help integrate dynamic formulations that take into account early losses and subsequent internal and external stress as factors that can predispose to or precipitate major depression, and the observations that depressed subjects often show hypercortisolism, significant anxiety, anorexia, diminished libido, hypo- or hyperactivity, and respond at times to limbic anticonvulsants.

Synthesis and Characterization of Small Conjugated D-π-A Molecules Containing Benzotriazole Units for Photovoltaics

정희연,김태동 한국고분자학회 2015 한국고분자학회 학술대회 연구논문 초록집 Vol.2015 No.10

한국판 리버풀 항정신병약물 부작용 평가 척도(LUNSERS)의 표준화 연구 - 리버풀 항정신병약물 부작용 평가 척도 -

정희연,주연호,신현균,정은기,강웅구,노명선,김용식 대한신경정신의학회 2002 신경정신의학 Vol.41 No.1

연구목적: 본 연구에서는 항정신병약물 부작용을 자기 보고식으로 평가하기 위한 리버풀 항정신병약물 부작용 평가 척 도(Liverpool University Neuroleptics Side Effect Rating Scale, 이하 LUNSERS)의 한국판을 제작하여, 항정신병약물로 치료 중인 정신분열병 환자와 정상 대조군을 대상으로 타당도와 신뢰도를 조사하고자 하였다. 방 법: 번역 및 역번역과 전문가들의 감수 과정을 거쳐 51문항, 4점 척도의 한국판을 제작하였다. 항정신병약물을 복 용중인 62명의 DSM-Ⅳ 진단 기준 정신분열병 환자를 대상으로 일주일 간격으로 두 번 LUNSERS를 완성하 게 하였다. 두 번째 LUNSERS 평가 시 동시에 정신과 전문의가 UKU side effect rating scale(이하 UKU)로 부작용을 평가하였다. 대조군은 LUNSERS를 완성하였다. 결 과: LUNSERS의 검사-재검사 신뢰도(r=0.86, p<0.01)와 UKU에 대한 준거 타당도(r=0.81, p<0.001)는 높았다. 그러나 항정신병약물 용량과 부작용 점수와는 통계적으로 유의한 상관이 없었다. ROC 커브 분석 결과 부 작용 점수 총점이 불특정 증상 총점보다 더 정확하게 항정신병약물에 의한 부작용을 보이는 환자를 감별할 수 있었다. 결 론: 한국판 LUNSERS의 신뢰도와 타당도가 검증되었고, 임상 연구에서 항정신병약물에 의한 부작용의 정도를 체계적으로 평가할 때 UKU를 대신하여 사용할 수 있는 유용한 도구임을 알 수 있었다. Objectives:This study was conducted to develop the Korean version of Liverpool University Neuroleptic Side Effect Rating Scale(LUNSERS) for measuring neuroleptic side effects by self-rating method and to examine the reliability and validity in the schizophrenic patients medicated by neuroleptics and normal controls. Methods:We made 51-item, 4-point scale of Korean version LUNSERS through translation, reverse translation and supervision by specialists. Sixty two schizophrenics diagnosed by DSM-Ⅳ criteria and medicated with neuroleptics completed LUNSERS twice with one week interval. Second LUNSERS and UKU side effect rating scale(UKU) by psychiatrist were administered to the schizophrenics at the same time. Normal controls also completed LUNSERS. Results:The test-retest reliability(r=0.86, p<0.01) of LUNSERS and the concurrent validity(r=0.81, p<0.001) against UKU were good. But the neuroleptic doses and total scores of side effect items didn’t show significant correlation. By the ROC curve analysis, the total scores of side effect items differentiated the medicated patients from non-medicated controls but not for the red herring items. Conclusion:Korean-version of LUNSERS has good reliability and validity. And it was also proved to be an useful assessment tool for measuring the extent of neuroleptic side effects systematically instead of UKU in clinical trials

질투망상과 성욕

정희연 순천향대학교 1993 논문집 Vol.16 No.4

After studying the relationship between the characteristics and sexual drive of patients with delusion of jealousy, we concluded the followings. 1) Number of patients with delusion of jealousy(patient group) was male 33, female 6, revealing male dominance than control group(schizophrenic patients without jealousy). 2) Mean age of patient group was greater than that of control group. 3) Patients' sexual drive state, as estimated by spouses and patient themselves, right before the onset of symptom, and sexual drive change after the onset of symptom in patient group were not different from control group. 4) Spouses' sexual drive states, as estimated by patients and spouses themselves, right before teh onset of symptom, and sexual drive changes after the onset of parients' symptom did not differ from that of spouses' of control group. 5) Frequency of sexual contact just prior to the onset of symptom in both patient and control group was not different. 6) Delusion of jealousy was mostly motivated by change of attitude and suspected extramarital problem of spouses, and the ratio was 30.6% and 25.0% each. 7) Premorbid personality type of patient group was not different from that of control group, but other than introverted and extroverted, paranoid type was most common. 8) Except for introverted, extroverted and dependent type was most common among personality types of spouses of patient group. 9) Among the diagnoses of patient group, alcohol intoxication, schizophrenia and delusional disorder were most common.

假神經症性 精神分裂症의 臨床的 硏究

정희연,곽동일 대한신경정신의학회 1976 신경정신의학 Vol.15 No.4

In this clinical study, it was intended to reveal the clinical characteristics of pseudoneurotic schizophrenia. Frequency of pseudoneurotic schizophrenia, age and sex distribution, birth order, educational level, marital status, religious background, socioeconomical status, occupation, family history, parental deprivation, premorbid personality, age of onset, predisposing factor, mental symptorr, organ of psychosmatic symptom and distribution of sympton were analyzed. Nature of hypochondriasis and phobia, duration of treatment, result of treatment, past history of treatment and experience of episodic psychosis were also examined. The subject of the study were 124 cases of pseudoneurotic schizophrenia out of 8,160 cases of out patients who visited the Department of Neuroᅳ psychiatry, Korea University hospital from Jan. 1971 to Oct. 1975. The result were as follows. 1. The frequency of schizophrenics occupied 9.36% of all out patients and the frequency of the latetnt schizophrenics occupied 3.00% of all out patients and the frequency of the pseudoneurotic schizophrenics occupied 1.51% of all out patients. 2. The mean age of the patients was 31.82 years old. As to the sex distribution, it was revealed that male occupied 58.87% and female occupied 41.13% of all patients. 3. There was no specific correlation with birth order. 4. The educational level of the patients were revealed higher than other psychosis. 5. According to the occupational distribution, among the male patients, student showed highest rate of 21.93% and among the female patients, housewife showed the highest rate of 62.3%. While 75.00% of them recorded as having certain kind of occupation it was revealed in careful observation that most of them either did poorly in their occupational role or frequently suspended due to their adjustment difficulties. 6. Family history revealed that 11.20% of their family members suffered from psychosis 7. As to the rate of parental deprivation before 15 years old, the rate of father deprivation was 14.52% and the rate of mother deprivation was 9.68%. 8. The mean age of onset was 26.6 years old, clearly older than other clinical types of schizophrenia. 9. Clear precipitating factors were elicited in 85.48% of them. The most frequent precipitating factors were those experience which were recognized as physical threats (31-46%).. The next frequent ones were personal seperation (27.42%) and frustrations associated with sexual experiences (16.13%). 10. Among the menta] symptoms, anxiety was 37.29%, hypochondriasis was 29.84%, depression was 13.71%, phobia was 12.90% and obsession was 7.26%, Most of their somatic complaints were rather bizarre. The most frequently cbosen bodily past and organs of their somatic complaints were head (33.87%) and cardiorespiratory organs (32.26%). 11. As to the nature of the hypochondrical nature, localized bizarre sensory nature was 37.84% and moving nature 35.14% As to the object of the phobia, the person occupied 50.00% and the death 31.25% 12. As to the duration of illness, 21.77% of the patient had the duration between 6 month and 1 year. 13. As to the past experience of their treatments 78.57% of the patient had taken drug from phar macist s shop and 75.00% had the other medical treaetment and; 69.64% had taken herb drug and 62.50% had psychiatric treatment. In the duration of the treatment from one month, to six monthes was 21.42% in the cases of psychiatric treatment less than one month was 66.07% in other medica:l treatment and intermittant herb and self-medicated drug users are 32.14% and 37.50% reciprocally. 14. Five of them (4.03%) had psychotic episodes. 15. The result showed many clinical significant differences comparing to the other schizophrenia, those are, age of onset is older, level of education is higher, tendency of superficial adaptation to their occupation is more frequent, prominent precipitating factors are more easil

정상 노인의 수면

정희연 순천향대학교 1993 논문집 Vol.16 No.2

The author reviewed sleep in the normal aged. The results obtained were as follows: 1. In waking EEG in the aged, there are the tendencies of decreased alpha frequency, decreased alpha amplitude and increased beta or fast activity. 2. In sleep EEG in the aged, there are decreased delta wave, decreased sleep spindle amplitude, decreased sleep spindle frequency and decreased frequency, amplitude, amount of alpha activity. 3. In sleep pattern in the aged, there are decreased total sleep, decreased slow wave sleep, decreased REM sleep and others. 4. In circadian rhythm in the aged, there are increased daytime sleep, decreased nocturnal sleep period. Elderly persons are reported to tall asleep and awake earlier, that is phase advance of circadian rhythm.

산조인이 백서 뇌에 미치는 영향 - 세로토닌, 수면시간, 수면뇌파, 자동운동을 중심으로 -

정희연,권영준,박인준,권준택,한병훈,이성필,Jeong, Hee-Yeon,Kwon, Young-Joon,Park, In-Joon,Kwon, Joon-Taek,Han, Byung-Hoon,Lee, Sung-Pil 대한생물정신의학회 1997 생물정신의학 Vol.4 No.1

Objects : Sanjoin, the seeds of Zizyphus vulgaris var. spinosus has been used as the most important hypnotic agent in chinese medicine to treat insomnia. This research was performed in order to examine the effect of betulinic acid and sanjoinine-A which are components of Sanjoin. Method : Sleeping time, sleep recordings of EEG, EMG, serum serotonin level, and locomotor activity were measured in rats which received betulinic acid and sanjoinine-A as sleep induction material extracted from Sanjoin. Results : 1) Groups received betulinic acid, sanjoinine-A, and lorazepam showed increased sleep time than control group with saline. 2) Groups with betulinic acid, sanjoinine-A, lorazepam and saline recorded ${\beta}$-wave in sleep recordings of EEG. In EMG, there was no significant difference among all groups. 3) No significant difference in serum serotonin level among all groups was found. 4) In autonomic activity testing, groups of betulinic acid, sanjoinine-A, and lorazepam showed significantly more decreased in activity than saline group. In comparison of groups of betulinic acid and sanjoinine-A with a group of lorazepam, there was no significant difference. Conclusion : These results suggest that betulinic acid and sanjoinine-A have the sedative effect like lorazepam rather than sleep effect.

ISONIAZID

정희연 대한신경정신의학회 1988 신경정신의학 Vol.27 No.4

정신분열병 환자에 있어서 가족력과 임상유형(Type I, Type II)에 따른 안구추적운동의 차이

정희연,임두원,권영준,주경수,서미경,김동수,Jeong, Hee Yeon,Rheem, Doo Won,Kwon, Young Joon,Joo, Gyung Soo,Seo, Mi Kyoung,Kim, Dong Soo 대한생물정신의학회 1995 생물정신의학 Vol.2 No.2

Smooth pursuit eye movement, one of the reliable biological markers of schizophrenia, is not always abnormal in schizophrenic patients. Therefore the authors studied it in schizophrenic patients and normal controls and compared the results according to the presence or abscence of family history of psychosis and types (type I and type II). The results are as follows: 1) In the 18 normal control group (8 mole, 10 female), there was no sex difference in the responses of smooth pursuit eye movement. 2) In th 44 schizophrenic group (28 male, 16 female), there also was no sex difference in the responses of smooth pursuit eye movement. 3) In comparison of 44 schizophrenic group to 18 normal control group, there was significantly increased abnormal response in smooth pursuit eye movement in schizophrenic group (P < 0.005). 4) In schizophrenic group, there was no difference in the responses of smooth pursuit eye movement between type I and type II schizophrenia. 5) The presence or abscence of family history of psychosis made no difference in the responses of smooth pursuit eye movement in schizophrenic group. 6) Subdivision of type I or type II in each case of presence or abscence of family history made no difference in the responses of smooth pursuit eye movement in schizophrenic group.