김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

비만 개선 효과를 지닌 탄수화물 및 지방 흡수 억제 기능성 식이조성물 개발

윤유식,최선미,홍순복,홍정미,김정원,이홍석,홍성길 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

In a previous study, we developed a new food additive as an egg yolk antibody (IgY) against carbohydrate digestion enzymes for the regulation of blood glucose level and weight control. The IgY delayed and decreased the increment of blood glucose level after administration of sucrose in human being by 30% in 20∼30 min. We also developed a lipase inhibitor as a water extract of two kinds of herb, Platycodon grandiflorum and Solanum melongena. Twenty three volunteers were subjected to the intake of the egg yolk IgY plus the herbal extracts for 50 days. In average, the treated subjects appeared to lose 1.96 ㎏ of body weight and 3.4 ㎏ of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of egg yolk IgY and natural herbal extract improve the obesity by the decrement of body weight and body fat mass.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

불규칙파를 이용한 파랑에 의한 회절에 관한 연구

이홍식,최창수 中央大學校 建設環境硏究所 1994 環境科學硏究 Vol.5 No.-

This research was performed the diffraction of irregular waves due to the semi-infinite breakwater. As for the simulation of irregular waves, the directional spreading function was divided by 10, 20, and 36 respectively under the condition of a constant division M=10 of frequency spectrum. Also two kinds of parameter Smax=10 and 75 were adopted for investigating the dissipation effect in the sheltered region. As the results of comparing with division number of the directional function, some differences were found between N=10 and N=36, but no any remarkable variance were found in the comparioson between N=20 and N=36. As for Comparing with the values of Smax, the diffraction coefficient distribution for Smax=75 was lower than that of Smax=10, and also irregular diffracted wave heights were almost lower than regular waves. As the verification step, the results of diffraction wave height and period's ratio obtained by this study were compared with Goda's results, a good agreement was found between them. Therefore the method of the this study may be useful for estimating diffraction wave height and layout of breakwater.

경남지역의 토양 및 농작물중 게르마늄 함량

이성태,이영한,이홍재,조주식,허종수 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

양질의 기능성 농산물 생산을 위하여 게르마늄의 농업적 이용에 대한 기초 자료를 제공하고자 경남지역의 토양을 중심으로 지역, 지형, 토양유형 및 토성별 게르마늄 함량과 자연상태에서 재배한 농작물별 게르마늄 함량을 조사하여 그 분포특성을 검토한 결과는 다음과 같다. 토양중 게르마늄 함량을 분석할 결과 성남지역 논 토양 평균 함량은 0.24 mg/kg 이었고, 마산시, 진주시 및 하동군 지역이 0.30 mg/kg 이상으로 높았다. 지형별로는 홍적대지가 0.26 mg/kg으로 약간 높았으나 지형에 따른 큰 차이는 없었다. 토양유형별 게르마늄 함량은 보통답이 0.27 mg/kg으로 사질답과 습답에 비하여 높았으며, 토성별로는 미사질양토가 0.27 mg/kg으로 비교적 높았다. 일반농작물중 게르마늄 함량은 곡류>채소류>과일류 순으로 높았고, 쌀과 보리는 각각 약 68 및 48 μg/kg의 게르마늄을 함유하고 있었으며, 단감과 배는 각각 약 11 및 23 μg/kg으로 전반직으로 낮을 수준이었다. 채소류중 게르마늄 함량은 엽채류>근채류>과채류 순으로 높았으며 특히 상추와 열무중 게르마늄 함량이 각각 약 62 및 65 μg/kg으로서 높았다. 약용작물중 게르마늄 함량을 신선초(Angelica keiskei), 토천궁(Ligusticum chuanxiong), 인삼(Panax ginseng), 삽주(Atractylodes macrocephala), 백지(Angelica dahurica), 맥문동(Liriope platyphylla) 및 도라지(Platycodon grandiflorum)에서 100 μg/kg 이상으로 게르마늄 함량이 높았으며, 선학초(Agrimonia pilose) 및 일당귀(angelica acutiloba)의 함량은 각각 37 및 30 μg/kg으로 낮았다. To investigate the germanium content in paddy soil in Gyeongnam province, 310 paddy soil samples were collected at 19 areas in Gyeongnam, Ulsan and Busan. Contents of germanium in paddy soils were analyzed in different topographies, soil types and soil textures. Average content of germanium in Gyeongnam was 0.24 mg/kg, those of Masan, Jinju and Hadong were above 0.30 mg/kg. Germanium content with different topographies were no difference. Germanium contents in different soil types were 0.27 mg/kg in well adapted soil and 0.23 mg/kg in poorly drained soil. Germanium contents in different soil textures were 0.27 mg/kg in silt loam and 0.23 mg/kg in sandy loam. To determine germanium content on agricultural product in the field, content of germanium in cereals, vegetables and fruits were analyzed. Germanium content of agricultural product was high in the order of cereals>vegetables>fruits. In case of vegetables, germanium contents were high in the order of leaf vegetables>root vegetables>fruit vegetables. Germanium contents were high with 62 and 65 μg/kg in lettuce and young radish, respectively. To analyze the germanium content in medicinal plant, samples were collected from 19 medicinal plants at Hamyang areas. Germanium contents in Angelica keiskei, Ligusticum chuan wng, Panay ginseng and Atractylodes nacrocephala were relatively high with 100 μg/kg above.

Pit식 방파제에 의한 파랑의 회절에 관한 연구

이홍식,이보형,A. Neil Williams 中央大學校 建設環境硏究所 1997 環境科學硏究 Vol.8 No.-

Wave diffraction has been studied for the prediction of wave interaction due to a pit breakwater. The present model is based on the linear wave theory and boundary element approach. The fluid domain is divided into two regions; an interior region whose boundary consists of the projection of the outline of the pit and an exterior region consisting of the remainder of the fluid domain. By utilizing an appropriate Green's theorem in each region, a pair of simultaneous integral equations has been derived for the velocity potential and its normal derivative at the imaginary fluid interface between the two regions. These integral equations have been discretized, and resulting system of algebraic equations solved by standard matrix techniques. Based on this solution technique, diffraction patterns in wave fields have been shown by the different various pit geometries. Finally, a numerical example for a navigation channel has been illustrated to present the effect of wave decay. In accordance with the condition of each pit breakwater, wave heights in the direction of leeward can be found reduced to 5 ~ 10% for the incident wave height.

응급의료센터 과밀화 해소를 위한 환자 전원

이경룡,구홍두,박인철,김승환,장석준,심호식 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

To reduce the degree of overcrowding in level Ⅲ emergency care centers, authors have conducted a prospective study of patient who were transferred to level Ⅰ or Ⅲemergency center based on the non-emergent patient guideline currently used at the severance Hospital, level Ⅲ emergency center, during the period of 65 days from Jan. 1st 1995 to Mar. 6th 1995. Followings are guideline for non-emergent patient described by the Korean government low: 1) systolic blood pressure greater than 80/100 of normal. 2) respiration rate between 10 to 24 per minute. 3) pulse rate between 60 to 100 per minute. 4) body temperature between 36 to 37.5℃ by rectal. 5) relatively alert mental status. 6) patient not requiring emergency operation at the time of examination. The results were as follows: 1. Among the total 5,301 patients, 464 patients ranged from age of 1 to 82 years old were transferred to level Ⅰor Ⅱ emergency center after simple treatment. 2. Among the 464 patients, 276 patients were treated in expected hospital with medical problems in 101 (36.6%) patients, and 175 (63.4%) patients had surgical problems. 3. Among the 276 patients, 122 (44.2%) patients were admitted, 145 (52.9%) patients were discharged from the emergency room after adequate treatment. 4. Among the transferred patients, simple laceration (33.0%) were most common, acute gastroenteritis (12.0%), and simple contusion (10.5%) were followed. These results suggest that about 10% of patients visiting level Ⅲ emergency care center, can be safely transferred to nearest level Ⅰor Ⅱ emergency centers on the bases of non-emergent patient guideline described by Emergency Medical Service law. These guideline, if put into proper use, can reduce the degree of overcrowding problems in level Ⅲ emergency care center through out the country.

어항입구 부근에서 Pit식 방파제를 이용한 쇄파 발생억제 연구

이홍식,이보형 中央大學校 建設環境硏究所 1998 環境科學硏究 Vol.9 No.1

The effect of wave decay due to single pit breakwater has been studied for restraining from occurring breakers in the vicinity of a fishing harbor entrance. Firstly, by utilizing Green's theorem and boundary element approach, wave diffraction due to a pit breakwater is performed to investigate wave interaction with linear wave theory. Diffraction patterns in wave fields have been shown by the different various pit geometries. Secondly, by using an appropriate numerical model based upon the time-dependent analysis including wave refraction, diffraction, and partial reflection is adopted to study the effect of various pit geometries near to a harbor entrance. Application of various pit breakwaters for a fishing harbor been illustrated to present the effect of wave decay. Finally, in accordance with the condition of each pit breakwater, wave heights in the direction of leeward can be found reduced to 10~20% for the incident wave height.

보리 춘파재배 적응성 품종의 특성 및 선발

이춘우,구본철,백성범,손영구,김홍식 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.1

보리 품종 중 춘파에 적응하는 품종을 선발하기 위하여 올보리 등 16품종을 시험한 결과를 요약하면 다음과 같다. 가. 천립중과 설립량과는 상관관계가 있어 천립중이 무거울수록 10a당 설립이 적고 수량은 많아지는 경향이었다. 춘파 재배는 생육기간이 짧고 고온하에 등숙이 이루어지므로 천립중이 가벼워지는데 대립종이 소립중에 비하여 설립이 적었다. 나. 수량은 맥종간에는 쌀보리가 겉보리나 맥주보리에 비하여 적었고, 또한 년차나 품종간 변이도 컸다. 다. 쌀보리 품종들은 겉보리에 비하여 간장이 짧고, 설립이 많아 수량이 적었다. 라. 춘파재배용으로는 천립중, 설립량, 간장, 성숙기, 수량 등을 고려할 때 올보리와 사천6호가 가장 안정성이 있는 품종으로 판단되었다. This study was conducted to select varieties for spring sowing in barley. The accumulated and the average temperatures affected the 1,000-grain weight and unmatured grain yield. There was a negative correlation between 1,000-grain weight and the yield of unmatured grain. The 1,000-grain weight became lighter because of short growth period and maturing in high temperature under spring sowing condition. Compare with the covered and two-row barley, the grain was lower than naked barley. Among 16 barley varieties. "Olbori", "Sacheon6" were well adapted to spring sowing condition.

항파상풍 톡소이드 주사후 항파상풍 항체 역가

이한식,하영록,구홍두,장석준,심호식,김승환,이정운 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Objective : Tetanus decreased in prevalence since the systemic vaccination began in 1940s. Despite of the improvement in treatment like critical care with ventilatior or antibiotics, the mortality rate resides around 45%. Currently the tetanus prevention protocol recommends immunization in infancy and boosters every tenth years. Thereafter the immunization with toxoid only or toxoid plus immunoglobulin injections is recommended according to the type of injury. In most of ED in Korea, only tetanus immunoglobulin is given without any basis. Previously we proved the effect that passive immunization with the immunoglubulin 250 IU last long for only a month. At this time we measured the effect of the active immunization with toxoid and its effect was compared to the effect of the tetanus immunoglobulin to find out the logical tetanus prevention after an injury. Method: 20 healthy adult volunteers were injected with tetanus toxoid and their anti-tetanus antibody titers measured before the injection, 1 week and 4 weeks after the inection. No volunteers have taken any medication for chronic illness(e.g. hepatits, tuberculosis) or had an tetanus immunization or booster in the last 6 months. Antibody titers were measured by IMMUNOZYM??-TETANUS and t-test was performed on the results, Results: 1. Total 20 volunteers(12 males and 8 females) were participated. 2. Subjects` ages were 21 to 44 years old and there were no relevance to the antibody titer. 3. The antibody titers of before the anti-tetanus toxoid injection and 1 week, 4 weeks after the injection revealed significant difference and the antibody titers of the first and the forth week after the injection also showed a significant difference. Conclusion: Currently in Korea, the tetenus immunoglobulin 250 IU given alone as tetanus prevention was injected, but it did not elevate the anti-tetanus antibody titer for 4 weeks where as tetanus toxoid injected intramuscularly did significantly. Therefore, tetanus toxoid should be given on the dirty injuries with additional tetanus immunoglobulin injection to accomplish the correct method of tetanus, tetanus toxoid prevention.