서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Dong-Chun Lecture : Cell-based High Content Screening (HCS) for action modes of drug targets by using systematic deletion mutants of Schizosaccharomyces pombe

( Dong Uk Kim ),( Im Seon Woo ),( Mi Sun Won ),( Young Joo Jang ),( Kyung Sook Chung ),( Han Oh Park ),( Hyang Sook Yoo ),( Kwang Lae Hoe ),( Doo Sik Kim ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

Ⅴ. 속담(俗談)ㆍ수수께끼편

임동권(Im Dong-Kwon),박현국(Park Hyun-Kook),김정원(Kim Jung-Won),장정순(Jang Jong-Soon) 중앙대학교 한국문화유산연구소 1995 중앙민속학 Vol.- No.7

Mito-Tempo Can Protect Against the Allergic Airway Inflammation through Reducing Mitochondrial ROS Generation

( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Mi Ran Kang ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Oxidative stress is well known to be implicated in the development of asthma. The mitochondrial respiratory chain is a major site of intracellular reactive oxygen species (ROS) generation and, at the same time, an important target for the damaging effects of ROS. Mito-Tempo is a specific mitochondrial ROS inhibitor and it is known to be associated with opening of mi-tochondrial permeability transition pore and inhibition of cell necroptosis or apoptosis. However, there is little information on the protective effects of Mito-Tempo on the inflammatory airway disorders including bronchial asthma and its acute exacerbation. We investigate the effects of Mito-tempo on the allergic airway inflammation and hyperresponsiveness using the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice). The OVALPS-OVA mice showed the typical features of neutrophilic asthma; increased airway inflammatory cells, the pathologic changes, the increased levels of Th2 cytokines in lungs of OVALPS-OVA mice, increased mitochondrial ROS generation, and increased bronchial hyperresponsiveness. Interestingly, we found that in OVALPS-OVA mice, Mito-Tempo, a novel mitochondrial targeting agent significantly reduced the increases in inflammatory cytokines, mitochondrial ROS generation, airway inflammation, and bron-chial hyperresponsiveness. These findings indicate that mitochondrial dysfunction including oxidative damage may be im-plicated in the pathogenesis of bronchial asthma and provide the therapeutic potential of a mitochondrial targeting agent, Mito-Tempo, for bronchial asthma.

The Relationship between Mitochondrial ROS and ER Stress in Allergic Airway Diseases

( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Mi Ran Kang ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Hee Jung Kim ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Mitochondria and the Nox family of NADPH oxidase are the two major sources of reactive oxygen species (ROS) that are induced by external stimuli, and the mitochondria respiratory chain is considered as an important site of ROS production within most cells. Recent evidence has demonstrated that various biological stimuli increase accumulation of unfolded or mis-folded proteins in ER lumen, which is referred to as "ER stress". Moreover, these various pathologic stimuli have been reported to provoke oxidative stress as well as ER stress. In this study, we used the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice) for elucidation of the relationship between mitochondrial ROS and ER stress in bronchial asthma. The OVALPS-OVA mice showed that the expression of ER stress markers and the protein levels of un-folded-protein response (UPR)-related marker in lung tissues were significantly increased after OVA challenge. In addition, we visualized the localization of mitochondrial ROS in BAL cells isolated from OVALPS-OVA mice using confocal microscopy; the significant increase in mitochondrial ROS in BAL cells was observed after OVA challenge. Our results also showed that Necrox-5 or 4-PBA significantly reduced the increases in ER stress, mitochondrial ROS, inflammatory cytokines, airway in-flammation, and bronchial hyperresponsiveness. These findings suggest that mitochondrial ROS and ER stress plays an im-portant role in the induction and maintaining allergic airway diseases synergistically.

반코마이신 내성 장내구균 분리 환자에서 황색포도알균의 내성

김동욱,신선혜,김혜진,류선,이선희,장철훈,김영대,곽임수 대한감염학회 2004 감염과 화학요법 Vol.36 No.1

목적 : Vancomycin-resistant enterococci (VRE)로부터 S. aureus로의 vacomycin 내성 유전자의 전파는 오래전부터 예견되어져 왔으며 큰 걱정거리로 남아있다. 최근에 vanA 유전자를 가지고 있는 vancomycin-resistant S. aureus 2주가 미국에서 분리되었다. 이 연구의 목적은 VRE를 보균하고 있는 환자들에서 S. aureus 보균양상과 항균제 내성을 알아보는데 그 목적이 있다. 재료 및 방법 : 2001년 1월부터 2001년 12월까지 부산대학교병원에 입원하였던 환자들 중 임상검체에서 VRE가 분리된 환자 7명과 2001년 9월부터 12월까지 외과 중 환자실에 입원하였던 환자들 중 VRE 감시배양을 시행하였던 20명의 환자들을 대상으로 하였다. 환자들은 전비공, 액와부, 회음부, 직장에서 매주 면봉으로 검체를 채취하였다. 선별배지에서 검체를 배양하여 S. aureus를 분리하였고 원판 확산법을 이용하여 methicillin 감수성을 측정하였다. Broth microdilution 법으로 vancomycin MIC를 측정하였다. 결과 : 총 27명에서 73회의 방문과 292회의 배양이 시행되었으며 총 67주의 S. aureus가 분리되었다. 이중 64주(95.5%)가 methicillin 내성이었다. 전비공 MRSA 보균율은 VRE를 보균하고 있었던 환자는 19명 중 11명(58%)에서, VRE를 보균하지 않았던 환자는 8명 중 3명(37.5%)으로 VRE 보균자에서 보균율이 더 높았다. 총 64주의 MRSA의 vancomycin MIC의 분포는 0.5-2㎍/mL였으며 2㎍/mL를 초과하는 균주는 발견되지 않았다. 64주 중 vancomycin MIC가 1㎍/mL인 균주가 54주(84.4%)로 가장 많았으며 2㎍/mL 6주(9.4%), 0.5㎍/mL 4주(6.3%)의 순이었다. 결론 : VRE 환자에서 S. aureus의 보균율은 비보균자 보다 높았으며 분리된 균주들은 대부분 MRSA였다. VRSA 균주는 발견되지 않았다. Background : The transfer of vancomycin resistance from vancomycin-resistant enterococci (VRE) to Staphylococcus aureus has been predicted. The purpose of this study is to determine the prevalence and antibiotics resistance of S. aureus among patients colonized with VRE. Methods : Between January 2001 and December 2001, a prospective study was performed at Pusan National University Hospital on 27 patients. Surveillance swabs from nasal cavity, axilla, perineum, and rectum were obtained at weekly intervals. Methicillin susceptability of S. aureus was determined by oxacillin disk diffusion test and minimum inhibitory concentration (MIC) for vancomycin by microdilution broth test. Results : Total of 292 swab cultures were performed and 67 S. aureus isolates were collected. 64 isolates (95.5%) were resistant to methicillin. The prevalence of nasal MRSA carrier in 19 patients colonized with VRE was higher than that in 8 patients not colonized with VRE (58% vs. 37.5%). In 64 MRSA isolates, MIC (㎍/mL) for vancomycin ranged from 0.5 to 2. No isolates with MIC >2 ㎍/mL were observed. MIC of 1 ㎍/mL was shown (observed) in 54 isolates, 2 ㎍/mL in 6 isolates, and 0.5 ㎍/mL in 4 isolates. Conclusion : The prevalence of S. aureus with colonization of VRE is higher than that without colonization of VRE. Most of S. aureus isolates were resistant to methicillin. VRSA isolates were not observed.

클로르헥시딘 바니쉬의 적용이 교정용 브라켓의 전단접착강도에 미치는 영향

임동혁,김태우,장영일,남동석,양원식,백승학 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.2

본 연구의 목적은 클로르헥시딘 처리가 교정용 브라켓의 전단접착강도와 탈락 양상에 미치는 영향을 비교하는 것이다. 실험군으로 4주간 4회의 클로르헥시딘 바니쉬를 도포한 22개의 사람 소구치를 선택하였고, 대조군으로 아무 처리도 하지 않은 22개의 사람 소구치를 선택하였다. 모든 치아는 37%의 phosphoric acid gel로 부식시키고 교정용 브라켓을 같은 조건으로 부착시켰다. 전단접착강도의 측정을 위해 Instron universal testing machine(model 4466)을 이용하였고, 법랑질과 브라켓 표면은 SEM으로 관찰하였다. 결과의 분석은 t-test를 이용하였으며 결과는 다음과 같다. 1.클로르헥시딘의 법랑질 처리는 전단접착강도에 영향을 미치지 않았다. 2.클로르헥시딘으로 법랑질을 처리한 경우와 그렇지 않는 경우에 브라켓 탈락 양상에 차이가 없었다. 따라서, 클로르헥시딘으로 법랑질을 처리하여도 브라켓 접착에 유해한 작용이 없었고, 따라서 임상사용에 적합하다고 생각된다. The purpose of this study was to determine whether the application of chlorhexidine varnish affects the shear bond strength and failure pattern of orthodontic brackets or not. The experimental group consisted of 22 human premolars which extracted after chlorhexidine vanish application(4 times for 1 week interval) in vivo, and the control group consisted of 22 human premolars which extracted without any pre-treatment. After all teeth were etched with 37% phosphoric acid gel, metal orthodontic brackets (Q-3002, RMO, USA) were bonded to each tooth using auto-polymerizing orthodontic resin (Ortho-One, Bisco, USA) with the same bonding procedure. The shear bond strength was measured with Instron universal testing machine (model 4466, Instron Ltd., England), and the failure pattern of each bracket was examined with Scanning Electron Microscope (SM 840A, JEOL, Japan). The data were analysed statistically with t-test. The results were as follows : 1.Application of chlorhexidine varnish had no significant effect on the shear bond strength of the orthodontic bracket. 2.There was no significant difference in the failure pattern of orthodontic bracket between the experimental group and the control group.

效果的인 잔骨材率(s/a)의 減少에 관한 實驗的 硏究

任晶淳,朴東俊,金鎭大 경기대학교부설산업기술종합연구소 1990 산업기술종합연구소 논문집 Vol.6 No.-

The natural aggregates of main materials in concrete, such as sand and gravel, are exhausted, due to the increase of large scaled construction works. It therefore leaves us badly in need of the man-made aggregates. While we can use the stone which has been crushed as the coarse aggregate, we still depend on the sand as the fine aggregate. As the results of the effective reduction of fine aggregate content(s/a), we can obtain cheaper concrete by saving natural resourses and cement. The material test consists of two kinds of water cement ratio(w/c;45%, 40%) and each has four classes of unit cement:400㎏, 360㎏, 320㎏, 280㎏, each of which has five classes of fine aggregate content; 45%, 40%, 35%, 30%, 25% with annexed Fly ash of ten percents of cement weight. The results obtained from the data in this test are summarized as follows; 1. The maximum compressive strength of concrete(??) shows around 35% of s/a 2. We can notice more effective reduction of s/a in poor mixing concrete(below 320㎏ of unit cement). 3. It is advisable to use adequate Fly ash and admixture agents to improve the consistency and the bond strength of concrete.

네일링에 의한 기초의 지지력 증대에 관한 연구

임종철,하용봉,공영주,김태오,김동희 釜山大學校生産技術硏究所 1999 生産技術硏究所論文集 Vol.56 No.-

구조물의 하중은 기초지반에 의해 지지된다. 그러나, 기초지반이 지하수의 침투 등에 의해 강도가 저하되거나 구조물의 증축에 의해 지지력이 부족할 경우가 발생한다. 이러한 경우 지반을 보강하기 위해 지반개량 공법이 적용된다. 지반개량 공법 중 네일링 공법은 시공이 간편하고 경제적이며 지반의 인장강도를 증가시키는 역학적 장점 등이 잇어 앞으로 널리 사용될 수 있는 공법이다. 본 연구에서는 네일을 이용하여 기초지반을 보강하여 지반의 강성을 높이고 지반의 지지력을 증대시켜서 구조물의 침하를 억제하는 역학적 기구를 규명하기 위해서 실내모형실험을 실시하였다. 본 연구의 목적은 시래모형실험을 통하여, 기초지반 보강용 네일의 설치 각도, 길이, 배치 간격의 변화에 따른 지지력 증대 효과 및 침하억제효과를 분석하여, 최적의 보강방법을 결정하는 것이다. The loads of structure are supported by foundation ground. But the bearing capacity will be able to be lacked by the decrease of ground strength by seepage of ground water or by the extension work of building. In this case, ground improvement method can be applied to increase the ground strength. Among ground improvement methods, nailing method will be used in the future widely, because of its simplicit, economical and mechanical merits of increasing tensile strength on ground. In this study, laboratory model tests are made in order to analyze the effect of ground improvement by nailing method. The final purposes of this study are as follows; (1) The effect of bearing capacity increasing by nails. Especially, the effects of distance, length and angle of nails. (2) To decide the optimal reinforcing methods of nailing

조직시민행동에 영향을 미치는 선행변수 도출을 위한 다원적 접근

林昌喜,이동규,김영천 홍익대학교 경영연구소 2001 경영연구 Vol.26 No.-

The main purpose in this study is to investigate relationship between exchange(economic and social) in organization and organizational citizenship behavior of employees. Two research questions are addressed in this study : (1) Do procedural justice, trust about supervisor, and empowerment positively associated with organizational citizenship behavior? and (2) What are the factors that more effective antecedents of organizational citizenship behavior? This study Blau(1964)'s "social exchange theory" to derive theoretical background, and consider that organizational citizenship behavior is explained economic exchange theory and social exchange theory. We considered procedural justice as economic exchange variables, and trust about supervisor and empowerment as social exchange variables. Data obtained from a survey of 205 employees of 33 korean firms is used to construct final variable measures and test the hypothesized relationships. The statistical results show that procedural justice, trust about supervisor, and empowerment are positively associated organizational citizenship behavior, and that derives procedural justice and trust about supervisor are more effective antecedents than procedural justice.