A selective cyclin-dependent kinase 4, 6 dual inhibitor, Ribociclib (LEE011) inhibits cell proliferation and induces apoptosis in aggressive thyroid cancer

Lee, Hyun Joo,Lee, Woo Kyung,Kang, Chan Woo,Ku, Cheol Ryong,Cho, Yoon Hee,Lee, Eun Jig Elsevier 2018 Cancer letters Vol.417 No.-

<P><B>Abstract</B></P> <P>The RB-E2F1 pathway is an important mechanism of cell-cycle control, and deregulation of this pathway is one of the key factors contributing to tumorigenesis. Cyclin-dependent kinases (CDKs) and Cyclin D have been known to increase in aggressive thyroid cancer. However, there has been no study to investigate effects of a selective CDK 4/6 inhibitor, Ribociclib (LEE011), in thyroid cancer. Performing Western blotting, we found that RB phosphorylation and the expression of Cyclin D are significantly higher in papillary thyroid cancer (PTC) cell lines as well as anaplastic thyroid cancer (ATC) cell lines, compared with normal thyroid cell line and follicular thyroid cancer cell line. LEE011 dose-dependently inhibited RB phosphorylation and also decreased the expressions of its target genes such as <I>FOXM1, Cyclin A1,</I> and <I>Myc</I> in ATC. Furthermore, LEE011 induced cell cycle arrest in G0-G1 phase and cell apoptosis, and inhibited cell proliferation in ATC. Consistently, oral administration of LEE011 to ATC xenograft models strongly inhibited tumor growth with decreased expressions of pRB, pAKT and Ki-67, and also significantly increased tumor cell apoptosis. Taken together, our data support the rationale for clinical development of the CDK4/6 inhibitor as a therapy for patients with aggressive thyroid cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> pRB and Cyclin D were expressed high in aggressive thyroid cancer. </LI> <LI> LEE011 suppressed pRB and also decreased the expressions of its target genes in ATC. </LI> <LI> LEE011 induced cell cycle G1 arrest and apoptosis, and inhibited cell proliferation. </LI> <LI> LEE011 inhibited in vivo tumor growth with decreased expressions of pRB and Ki-67. </LI> <LI> We could explain the anticancer effects with the RB-E2F pathway. </LI> </UL> </P>

Plenary Session 2 : PS-2-1 ; Outcomes of hepatitis B virus recurrence after liver transplantation: a multicenter analysis in Korea

( Hee Yeon Kim ),( Jong Young Choi ),( Dong Goo Kim ),( Myoung Soo Kim ),( Soon Il Kim ),( Shin Hwang ),( Sung Gyu Lee ),( Kwang Woong Lee ),( Kyung Suk Suh ),( Young Seok Han ),( Dong Lak Choi ),( Se 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: The outcome of hepatitis B virus (HBV) infection after liver transplantation (LT) was improved by hepatitis B immunoglobulin (HBIG) and nucles(t)ide analogue (NUA). However, HBV recurrence after LT is critical because the recurrence is occasionally accompanied by a progressive destruction of graft and poor survival. The aims of this study were to investigate the significance HBV recurrence and identity factors associated with HBV recurrence. Methods: From October 1999 to February 2011, a total of 2684 consecutive LT recipients who underwent HBV-associated LT were retrospectively enrolled from 7 transplantation centers in Korea. Results: Prophylaxis regimens were HBIG monotherapy (67.7%) or a combination of HBIG with NUA (22.3%). The recurrence rate of HBV was 6.1% (164 recipients) during mean follow-up duration of 10.9 years. The median time from transplantation to recurrence was 2.1 years (0.1-7.9 years). Of the 1,071 patients with hepatocellular carcinoma (HCC) prior to LT, 155 patients (14.5%) had HCC recurrence after transplantation, and 48 patients (31.0%) had HBV recurrence. Of the 48 patients with recurrence of both HBV and HCC, 25 patients (52.1%) experienced HBV recurrence after HCC recurrence. In the multivariate analysis, pretransplant HCC, pretransplant HBV DNA above 5.5 log copies/mL was independent clinical factors influencing HBV recurrence after LT. The mortality rate among the recipients with HBV recurrence was 34.1% (56 recipients). Mean overall survival was 6.4 years in the HBV-recurrence group and 9.9 years in the HBV-nonrecurrence group (p<0.001). HBV recurrence was not an independent prognostic factor for overall survival. HCC recurrence was the most important factor for overall survival. Conclusions: The overall outcome of LT in HBV-related liver disease was excellent with the current prophylaxis regimen Choon Hyuck David Kwon,8 Suk-Koo Lee8 Pretransplant HBV DNA and HCC were important factors for HBV recurrence. HBV recurrence after LT did not significantly influence on the overall survival without combining of HCC recurrence.

Cytoreductive surgery with hyperthermic intraperitoneal chemotherapy in management of peritoneal carcinomatosis: Single center experience in Korea

Hee Ju Lee1,HyungJoo Baik1,박요한,서상혁,김광희,Ki Beom Bae,Kwan Hee Hong,김기향,변정미,Dae Hoon Jeong,Kyung Bok Lee,오민경,조광래,안민성 대한종양외과학회 2019 Korean Journal of Clinical Oncology Vol.15 No.2

Purpose: Peritoneal carcinomatosis (PC) has been considered a terminal condition and cytoreductive surgery with hyperthermic intraperitoneal chemotherapy (CRS/HIEPC) is regarded as an alternative therapeutic option. This study aimed to evaluate the 30-day clinical outcomes of CRS/HIPEC and the feasibility of the surgery by investigating the morbidity and mortality in Inje University Hospital. Methods: Data were retrospectively collected from 19 patients with PC who underwent CRS/HIPEC at Inje University Hospital in 2018. We evaluated pre-, intra-operative parameters and postoperative clinical outcomes and early complications. Results: The mean operating time was 506.95 minutes and the mean blood loss was 837.11 mL. Six cases (31.58%) had morbidity of grade III or above. A longer operating time (≥560 minutes, P=0.038) and large blood loss (≥700 mL, P=0.060) were positively correlated with grade III or worse postoperative complications. Conclusion: Our early experience with CRS/HIPEC resulted in a 31.58% morbidity rate of grade III and above, with risk factors being longer operating time and greater intraoperative blood loss. As the surgical team’s skills improve, a shorter operating time with less intraoperative blood loss could result in better short-term outcomes of CRS/HIPEC.

중년여성의 약물사용 실태 조사

이영선,김은경,김경숙,강경인,김희선,신성희,김은숙,최지선,신혜숙,황선기 여성건강간호학회 2001 여성건강간호학회지 Vol.7 No.4

The purpose of this study was to investigate the drug use of middle aged women. The subject consisted of 330 middle aged women who ranged in age from 40 to 60 years. They were selected in Seoul, Kyung-Ki province, Korea. Data were collected by using questionnaires, from April, 10th to 30th 2001 and analyzed by the SPSS PC+ program using qui-square. The results are summarized as follows ; 1. The proportion of drinking and smoking experience was 42.4%, 3.7%. The motivation of drug use was advised family and relatives(46.1%), doctor's order(39.4%), magazine and advertisement(14.5%). Most of middle aged women get the information on knowledge of drug from hospital(47.3%), magazines and advertisement(30.3%), advised family and relatives(22.4%). And 57.9% of the middle aged women didn't know side effect of the using drug and 13.9% of the middle aged women have had experienced with side effect. And the level of attitude on drug abuse in middle aged women was 43%. Most of the subjects(93.9%) didn't use alternative drugs, and they used more than 3 kinds of drugs(47%). 2. They used digestives(44.2%), applying ointments(41.8%), drinks(39.4%), analgesics(39.1%), laxatives(8.8%), anti hypertension drugs (8.8%), and anti-anemic drugs(8.8%) in their orders.

췌장 선방세포암 1예

이화정,지준호,박승찬,박정철,최은정,서혜진,이원식,이정림,배병조,손경락,이경희 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Acinar cell carcinoma is a rare tumor that represents 1~2% of al1 pancreatic cancers. Clinical and radiologic findings are inconclusive in this disease Acinar cell carcinoma is characterized by rapid progression and early metastasis, which lead to its poor prognosis. A 41-year-o1d man was admitted to our hospital for abdominal pain. Abdominal computed tomography (CT) and positron emission tomography-computed tomography (PET-CT) showed a splenic mass, which was being invaded by a pancreatic tail mass and which had increased ^(18)F-fluorodeoxyglucose (FDG) uptake Primary radical distal pancreatectomy and splenectomy were performed. Pathologic findings revealed an acinar cell carcinoma of the Pancreas The patient underwent a total gastrectomy three months later because of gastric recurrence Four months later, multiple hepatic metastases were discovered, and the patient underwent a left hepatectomy During treatment with capecitabine, there was no evidence of tumor progression for 14 months. We report a case of metastatic pancreatic acinar cell carcinoma, which did not progress for an extended period while the patient was being treated with capecitabme.

간호계와 비간호계 대학생의 AIDS관련 지식 조사연구 : 경기지역 일개 전문대 재학생을 중심으로

이애경,김영희 경복대학 1997 京福論叢 Vol.1 No.-

후천성 면역결핍 증후군(AIDS)는 전세계적으로 그 심각성이 더해가고 있으며, 인류의 복지를 위협하는 무서운 질병이다. 최근들어 한국에서도 HIV감염지수가 증가하고 있는 추세이며, 1996년말 현재까지 608명의 환자가 발생되었다. 그러므로 본 연구에서는 경기지역 일개 전문대 재학생을 중심으로 에이즈 예방교육을 실시하기에 앞서 그들이 갖고 있는 사전지식을 조사하고자 에이즈관련 지식에 대한 서술적 조사연구를 시도하였다. 연구대상자는 경기지역에 소재하는 일개 전문대 18세에서 26세까지의 재학생 242명으로 연구도구로 34문항의 질문지에 의하여 자가보고법으로 자료수집을 하였으며 실수, 백분율, 평균값, 유사성검정, t-test, ANOVA, 2-way ANOVA를 이용하여 자료를 분석하였다. 연구결과는 다음과 같다 : 1. 전체 대상자의 에이즈관련 지식점수는 평균17.67점이었고, 정답율의 범주는 47.2%-94.6%이었다. 2. 평균점수가 가장 낮은 문항은 '일단 에이즈균이 몸에 있으면 에이즈환자가 된다(47.2%)' 였는데, 이는 에이즈의 잠복기간을 잘 알고있지 못함을 보여주고 있다. 3. 비간호계 집단의 에이즈관련 지식정도는 간호계 집단에 비해 낮은 평균값을 보였는데, 이는 에이즈예방교육시 건강관련과목을 전공하지 않는 일반대학생들을 중심으로 하는 에이즈예방교육이 강조되어야함을 나타내주고 있다. 4. 대상자의 인구사회학적 특성에 따른 대상군의 에이즈관린 지식정도의 차이를 확인한 결과 전체 대상군에서 일반잡지를 통한 교육경험이 있는 집단이 교육경험이 없는 집단에 비해 지식점수가 높게 나타났으며, 비간호계 집단에서는 건강정보잡지를 통한 교육경험이 있는 집단이 교육경험이 없는 집단에 비해 지식점수가 높게 나타났고, 간호계에서는 통계적으로 유의한 차이를 보이지 않았다. 육이 강조되어야함을 나타내주고 있다. 4. 대상자의 인구사회학적 특성에 따른 대상군의 에이즈관련 지식정도의 차이를 확인한 결과 전체 대상군에서 일반잡지를 통한 교육경험이 있는 집단이 교육경험이 없는 집단에 비해 지식점수가 높게 나타났으며, 비간호계 집단에서는 건강정보잡지를 통한 교육경험이 있는 집단이 교육경험이 없는 집단에 비해 지식점수가 높게 나타났고, 간호계에서는 통계적으로 유의한 차이를 딘이지 않았다. Acquired immunodeficiency syndrome(AIDS) is threatened to human being's welfare is a far greater serious disease than most other disease. Recently HIV infectious clients are increasing tendency in Korea and the number of AIDS patients are 608 peoples in 1996. Therefore the purpose of this study was to identify the level of AIDS related knowledge before the eduaction of AIDS prevention of the college students at Kyung-gi area and the research design utilized in this study was a descriptive survey research. The subject were 242 college students where is at Kyung-gi area and they were 18-26 years old. The data were collected by self reporting with a questionnaire of 34 items and were analized by frequency, percentage, means, qui-square test, t or ANOVA test, and 2-way ANOVA. The results were as follows : 1. The Mean score of AIDS related knowledge was 17.67 and the correct answer rate of it was from 47.2% to 94.6%. 2. The lowest item was 'The person who has HIV get AIDS soon(47.2%).' and it was shown that the subject didn't know well about latent period of AIDS. 3. The mean score of a group who was majoring in non-nursing was lower than who was majoring in nursing and it must be empasized to AIDS preventive education for the group who was majoring of non-medicals. 4. The level of AIDS related knowledge as to demographic facter was revealed that the total group who had an learning experience through the magazine was high score of AIDS related knowledge than who had no experience, while the non-nursing group who had an learning experience through the jounal of health topics was high score of it than who had no experience., and it was not significant statistically in the nursing group.

Gilbert 증후군에서 열량 제한 시험과 Phenobarbital 자극 시험의 의의(14예)

이헌영,채경훈,정재훈,강윤세,김연수,문희석,박기오,이엄석,김선문,김석현,성재규,이병석,이강욱 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Gilbert 증후군은 인구의 7%에서까지 나타날 수 있는 매우 흔한 증후군으로서 비진행성인 양성의 만성적 경과를 치하며, 간질환의 증상과 징후가 없는 경한 비포합형 고빌리루빈혈증이 특징인 일종의 체질적인 증상으로서 혈장 빌리루빈 농도에 대한 사춘기의 영향 때문에 10대와 20대에 자주 진단이 된다. 따라서 임상적인 중요성은 미약하지만 높은 빈도가 예상되는 점에 그 중요성이 부여되어야 할 것이다. 따라서 적정한 임상적 진단법으로 기왕에 소개된 열량제한 시험과 phenobarbital 유도 시험을 시행하고 이들의 진단적 가치를 알아보기 위하여 본 연구를 시행하였다. 1990년 7월부터 1999년 4월까지 충남대학교병원에 내원하여 HBsAg, IgG anti-HBc 및 anti-HCV가 음성이고, 간 초음파 스캔에서 이상이 없으며, 혈청 AST, ALT 및 AP가 정상인 비음주자에서 경한 비포합형 고빌리루빈혈증이 있는 14예의 환자들을 대상으로 ^(99m)Tc-DISID 스캔을 시행하였으며, 기저 치 총빌리루빈 및 포합형 빌리루빈 치를 측정한 다음에 하루에 400Kcal로 48시간동안 제한한 열량 제한 시험을 시행하였고, phenobarbital을 하루 60mg씩 5일간 투여한 후에도 각각 총빌리루빈과 포합형 빌리루빈 치를 검사하여 비포합형을 구하였다. 대상 환자들은 모두 14예로서 남자가 11예(78.6%)였고 여자가 3예(21.4%)여서 3.7:1로 남자에서 많았으며, 20대가 6예(42.9%), 30대가 역시 6예(42.9%) 및 40대가 2예(14.2%)로서 2,30대가 대부분(85.8%)이었다. 열량 제한 시험 후의 총빌리루빈 치, 비포합형 및 포합형 빌리루빈 치들은 평균 각각 5.5±2.7, 4.2±2.3 및 1.3±10mg/dL 로서, 시험 전 치들인 3.0±0.8, 2.2±0.8 및 0.7±0.4mg/dL 보다 유의하게(p=0.001, p=0.001, p=0.023) 상승하였다. 포합형 빌리루빈 치도 유의하게 상승하였으나 비포합형의 상승보다는 훨씬 낮아서 주로 비포합형이 증가하였다. phenobarbital 투여 중 설사가 발생하여 중단한 1예를 제외한 13예에서 열량 제한 시험 후에 상승하였던 총, 비포합형 및 포합형 빌리루빈 치가 phenobarbital 유도 시험후에는 2.0±1.1, 1.5±0.8 및 0.5±0.4mg/dL로서 열량 제한 시험 결과보다 유의하게 낮아졌고(p=0.00, p=0.000, p=0.001), 열량 제한 시험 전의 기초치들인 3.0±0.8, 2.2±0.8 및 0.7±0.4mg.dL 보다도 더욱 낮아졌으며 유의한 차이(p=0.001, p=0.02, p=0.005)를 나타내었다. 14예에서 시행한 ^(99m)-Tc DISIDA 스캔에서 9예(64.3%)가 정상이었고, 5예(35.7%)에서는 심장 및 신장으로의 간외 섭취가 3예였고, 60분까지 소장 배출이 없는 배설 지연 예와 담낭 수축 불량 예가 각각 1예 씩 발견되었다. Phenobarbital 투여시험에서 민감도가 열량제한시험에 비해 더 높았다(92.3%와 50.0%). Gilbert 증후군에서 1일 400 Kcal로 48시간의 열량제한 시험과 1일 60mg의 phenobarbital을 5일간 투여하는 유도 시험은 편리하고 유용한 임상적인 진단법으로 이용할 수 있다고 생각된다. 그러나 열량 제한 시험에서는 증가 기준의 통일이 필요하다고 유추되며 phenobarbital 유도 시험이 민감도가 더 높은 것으로 생각된다. Gilbert's syndrome is very frequent and benign chronic process characterized by mild, intermittent, unconjugated hyperbilirubinemia without any symptom and sign of liver disease. Previously intoduced caloric restriction test and phenobarbital stimulation test as two appropriate clinical tests had been examined and their diagnostic values were reevaluated. Fourteen patients with mild, persistent, unconjugated hyperbilirubinemia were included. Subsequently caloric restriction has been applicated by 400 Kcal/day for 48 hours and phenobarbital has been prescribed by 60 mg/day for 5 days. Therafter serum levels of total and direct bilirubin were measured. Most of the patients were third and fourth decade(85.8%) and male predominant. Each basal serum levels of total, indirect and direct bilirubin were 3.0±0.8, 2.2±0.8 and 0.7±0.4 mg/dL. After caloric restriction test, each levels were increased significantly to 5.5±2.7, 4.2±2.3 and 1.3±1.0 mg/dL(p=0.001, p=0.001, p=0.023). After phenobarbital stimulation test for 13 patients had been practiced, increased levels of each bilirubin after caloric restriction test were decreased significantly to 2.0±1.0, 1.5±0.8 and 0.5±0.4 mg/dL(p=0.000, p=0.000, p=0.001) and these levels were significantly lower than basal levels(p=0.001, p=0.02, p=0.005). The sensitivities of caloric restriction test were 85.7%, 50.0%, and 71.4%, 35.7%(1.0, 1.5 mg increase of total bilirubin and 1.0, 1.5 mg/dL increase of indirect bilirubin). The sensitivities of phenobarbital stimulation test were 93.2% and 92.3% at criteria of 1.5 mg/dL increase of total bilirubin and indirect bilirubin. On the diagnosis of Gilbert syndrome, caloric restriction test and phenobarbital stimulation test are convenient and useful diagnostic tools in clinical face. And also phenobarbital stimulation test has higher sensitivity than caloric restriction test. Furthermore, standardization of bilirubin increment would be necessary in caloric restriction test.

Impedance를 이용한 전도성고분자 센서의 가스감응특성

이경문,유준부,이병수,전희권,이상태,이덕동,허증수 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

The Effect of Power Walking Exercise on Body Mass Index and Positive and Negative Symptoms in Patients with Schizophrenia

Lee,Jung Hee,Lee,Kyung Hee 계명대학교 간호과학연구소 2009 계명간호과학 Vol.13 No.1

Purpose : This study was designed to evaluate the effects of power walking exercise on Body Mass Index(BMI) and Positive and Negative Symptoms in inpatients with schizophrenia. Method : The study was conducted from February 1 to April 10, 2005 at the S mental hospital in Gimcheon. Sixty schizophrenic patients were allocated into intervention group (n= 30) and control group (n=30). Intervention group received routine care and power walking exercise 3 times a week for 8 weeks whereas control group received only routine care. Data were analyzed by descriptive statistics, χ2_test, and independent t-test using SPSS/WIN 11.0 version. Result : Hypothesis I, "the BMI of the intervention group will be lower than that of control" was supported(t=-6.845, p=.000). Hypothesis II, "the scores of positive and negative symptoms of the intervention group will be lower than those of the control" was rejected(t=-1.418. p=.161). Conclusion : Results of the study showed that power walking exercise was effective for the reduction of BMI but did not show statistically significant improvement in PANSS. Further study may be necessary to determine the long term effects of the current power walking exercise.

2자유도 PI 제어기를 이용한 BLDC 전동기의 벡터제어에 관한 연구

이종현,성낙규,이상집,이승환,오봉환,이훈구,한경희 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

This paper describes the speed control method of BLDC Motor using TDOF PI ( Tow-degree of freedom PI ). PI control method generally used has difficulty in efficient speed control because speed response and load torque response interfere each other. It can't decrease the overshoot of motor speed w rm, and shorten the response time of load torque TL, at the same time according to the step alternation of speed reference w rm. Therefore TDOF PI is applied to BLDC Motor in order to supply these weak points of PI control method in this paper. TDOF PI can get the torque characteristic of short response time as well as stable and prompt speed adjustment by separate control for speed response and load torque response. The results from the tests of C program simulation and system construction show that TDOF PI is superior to PI control system in speed adjustment and load torque response.