다중 중합효소 연쇄반응을 이용한 소의 Johne병 진단 기법 확립

김종배,송혜원,김근희,김홍,신광순,김두 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.1

반추수에서 발생하는 Johne병의 조기 진단 방법을 제시하고 이 질병의 원인체와 미생물학적 특징이 유사한 M. bovis, M. avium 등의 mycobacteria감염증을 감별 진단하는 방법을 개발하기 위하여 Mycobacterium 균속의 표준균주를 사용하여 중합효소 연쇄반응을 확립하였다. Johne병으로 의심되는 소의 혈액과 유즙을 채취하여 분리한 단핵구 및 거식세포로부터 genomic DNA를 추출하였다. 각 시료로부터 추출한 DNA를 template로 이용하여 Mycobacterium spp.에 특이적인 16S rDNA primer set를 이용한 PCR을 수행하여 시료내의 mycobacterial DNA 보유 여부를 확인하였다. 한편 mycobacteria 양성으로 확인된 시료는 M. avium complex 균종에 특이한 16S rDNA 염기서열을 기초로하여 제작한 primer set와 M. paratuberculosis 의 IS900 sequence에 특이한 primer set를 이용하여 duplex PCR을 수행하여 Johne병 원인체의 보균 여부를 조사하였으며, 이 결과를 oligonucleotide probe를 사용한 Southern blot hybridization을 통하여 다시 확인하였다. 이와 같은 duplex PCR 기법을 실제 축산 현장에서 수집한 유즙과 말초혈액으로부터 분리한 단핵구 및 거식세포 시료에 적용한 결과 본 연구에서 확립한 duplex PCR기법 유용성을 확인할 수 있었다. Diagnosis of Bovine Johne's Disease Using Multiplex Polymerase Chain Reactions In order to improve the early diagnosis of Johne's disease in ruminants, duplex polymerase chain reaction system for the detection of the etiologic agent of M. paratuberculosis and for the differentiation of other mycobacterial animal pathogens, such as M. bovis and M. avium, was applied. Genomic DNAs were purified from peripheral blood monocytes or milk macrophages and were used as templates in the duplex PCR. Detection of Mycobacterium spp. in the specimen was carried out by PCR using primer set specific to the mycobacterial 16S rDNA. And then, mycobacterial DNA-positive specimens were further differentiated with duplex PCR system which was composed of primer sets specific to 16S rDNA of M. avium complex and IS900 gene of M. paratuberculosis. The results were re-confirmed by Southern blot hybridization with oligonucleotide specific to the internal sequence of IS900 PCR amplicons. The applicability of this duplex PCR system was evaluated with DNAs extracted from clinical specimens of peripheral blood monocytes and milk macrophages. In summary, the duplex PCR amplification system described in this experiment is promising molecular technique for the early diagnosis of Johne's disease in ruminants.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)

김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.

아시아티코사이드로부터 메칠 2β, 3β-에폭시-23-히드록시우르스-12-엔-28-오애이트의 합성 및 이의 환원적 에폭시환 개열반응

주상섭,임두연,서성기,남태규,박형근,김희두,김창민,이민희,백형근,이민정,정영훈 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Steroidal 2β, 3β-epoxy compound was prepared from asiaticoside via six steps and reduced regioselectively with lithium aluminum hydride. Epoxide ring opening furnished 9 as a sole product at reflux condition through axial hydride attack at C-3.

생태적 도시계획을 위한 생태기반지표의 개발과 활용에 관한 연구(Ⅱ)

김현수,안근영,장대희 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

With environmental problems and pollutions accumulated by continuous urban development projects, it is becoming necessary to develop in consideration of environmental effects. On this study, it is suggested the critical point of biotope space factor and the supplement device. Natural ground area ratio is functioned as important factor in urban area. So in region, high-rated in natural ground area ratio, or in new urban development area, it is necessary to get supplement tool for conserving preferentially natural ground area. And it is considerable to ply individual environment planning indicator in case of need. This study has main contents as follows; biotope space factor was applied to actual case, rebuilding housing complex in Cham-sil to diagnosis ecological level and to lead more ecologically sound plan. After considering all the factors there was the critical point in using as just one tool, biotope space factor, so we suggested supplement device to solve this problem for an example, by regional characteristics, biotope space factor and individual environmental planning factor will be used together.

서울시 인구의 공간변화 경향에 관한 연구

金根永,강희진 강남대학교 산학기술연구소 2001 산학기술연구소논문집 Vol.- No.11

Population is an important socio-economic index that explains levels of urban development, current conditions of residential environments, locations of public and private facilities, and socio-economic activities of residents in a city or region. The Seoul city region with over ten million residential population(over 20% share of national population) within an area of 605.5km2(only 0.6% share of national area) has recently experienced the dynamic migration of city residents from central districts to suburb areas at the peak of 10.97 million population in the year of 1992. The objectives of this research are: (1) to investigate the dynamic patterns of Seoul residential population that is already declining with respect to five regional groups of central, northwest, northeast, southeast, and southwest regions provided by 『2011 Seoul City Regional Plan』, (2) to evaluate annual population changes of Seoul jurisdictional Dongs using statistical analyses, and (3) the spatial distributions of Seoul population using a geographic information system. To do this, this research collects annual, Dong-based population data from Seoul statistical yearbooks from the year of 1979 to 1999. This research also applied correlation analysis, hypothesis testing of two non-independent paired data, and spatial analysis using SAS v. 6.12 and Mapinfo v. 4.1. Results from general pattern investigation show that the population of Seoul region is declining since the year of 1992, though the population of the Seoul metropolitan region still increases. This declining population is not the spatial case in one area, but the widely observed, fundamental trend. Results from statistical analyses present that all of correlation analyses and most of hypothesis testing provided significant test outcomes within the 99% significance level. This means that the population number of Seoul city is dramatically changing during most of the study period. The spatial analyses using Mapinfo software clearly show the suburbanization pattern of Seoul population.

Monasucs anka의 적색조 생산 특성

김희구,박근태,손홍주 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.6

Monascus anka KFCC 4478의 적색소 생산력을 증가시키기 위하여 배지조성 및 배양조건에 대하여 검토하였다. 공시균주의 색소 생성력은 탄소원으로 glucose 4.0%, 유기질소원으로 peptone 3.0%, 무기질소원으로 NaNO_3 0.15%, 비타민 성분으로 thiamine-HCI 및 nicotinic acid 각 10㎍/l였으며, 배양온도 30℃, 초기 pH 7.0이었다. 특히 유기질소원과 무기질소원을 복합 처리함으로서 색소 생성력이 향상되었다. 결정된 색소생성 최적조건하에서 본 공시균주는 배양 5일만에 최대 균체 증식과 색소 생성력을 나타내었다. Optimal media and cultural conditions for the production of red pigment were established using Monascus anka KFCC 4478. The optimal temperature and initial pH for the production of red pigment were 30℃ and 7.0, respectively. Glucose turned out to be most suitable carbon source for red pigment production. Optimal glucose concentration was 3.0%. Addition combined of nitrogen sources of peptone and NaNO_3 induced good red pigment production. Thiamine-HCL and nicotinic acid were increased the production of red pigment. Under optimal conditions, maximum red pigment production and cell growth were observed after 5 days of incubation.

난분해성 폐수처리를 위해 Bioaugmentation한 생물막 반응기에 관한 연구

이건,김희,조순자,박근태,차미선,이상준 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-

The effect on the stability of biofilm and phenol removal efficiency under high phenol loading was investigated by RBC(Rotating Biological Contactor) using augmented reactor and unaugmented reactor with Rhodococcus sp. EL-GT. The operation of RBCs was carried out at rotating speed of 10 rpm and hydraulic retention time of 7 h at 20℃. The maximum amount of dry weight of the biofilm was 0.04 g/㎠ to running time for 15 days. The amount of attached biofilm of contactors was stabilized as 0.15∼0.03 g/㎠ for 35 days' running times and that of two reactors was similar value. But unaugmented reactor was shocked at 1,200 mg/ℓ phenol loading that the biofilm and removal efficiency were impacted. The bioaugmentation reactor showed higher removal rates than unaugmented reactors about phenol, sCOD and nitrogen contents. Bioaugmentation, with the strain originating from the same ecosystem and growing effectively on a selective substrate, was often effective means to treatment of recalcitrants like as phenol wastewater.

PWR에 使用되는 우라늄의 精製 및 濃縮에 關한 硏究(Ⅰ) : 國內産 우라늄 鑛石에서 黃酸浸出과 溶媒抽出에 依한 우라늄精製 Uranium concentration from the Domestic Uranium ores by Sulphuric Leaching and Solvent Extraction

金鍾一,朴永錫,丁梧鎭,李秀一,金熙峰,裵相龍,秋相根 조선대학교 동력자원연구소 1986 動力資源硏究所誌 Vol.8 No.2

This research was undertaken to study the mineralogy, and the optimum conditions for uranium leaching and extraction from the Okchon system graphite ores in the Kumsan areas. A research has been needed to study the refining process for the effective leaching and extraction from uranium ores in the Kumsan area which has more uranium deposits than any other area. By the mineralogical study with the powder X-ray diffraction method, primary uranium minerals are uraninite, brannerite, coffinite etc. and secondary uranium minerals are uranocircite, niobium uranium oxide, carnotite, toberite etc. has developed in the studied area uranium ores. The volumetric ratio of the primary and the secondary uranium minerals are approximatly 1 : 5. In this experiment, the uranium-bearing graphite in the Kumsan area was milled with a jaw crusher and ballmill. This milled uranium-bearing graphite was leached by sulfuric acid and from this sulfuric acid-leaching solution, uranium was extracted by Amberlite LA-1 in kerosene. The results are as follows: (1) The optimum results in the leaching process are : acidity pH = 1.0, temperature : 25.0℃, particle size : -80+120 mesh, leaching time : 24 hours, pulp density : 50% solid (2) A good pH range for uranium extraction was between 0.8 and 1.3. (3) The uranium distribution coefficient increases in direct proportional to the concentration of the Amberlite LA-1 as extractive agent. (4) The optimum time at 200rpm was 15 minutes.

토당귀 종자의 형태적 특성과 발아와 입모율에 대한 저장방법,생장조절제 및 프라이밍 처리 효과

안희정,안복주,·김도현,·안영섭,·김영국,박춘근,이상원,·박충범·차선우,·송범헌, 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1

This study was conducted to have basic and applied informations to establish the cultivation method and to improve the cultivation techniques of Angelica gigas Nakai through investigating the rates of germination and seedling establishment with treatment of plant growth regulator and seed priming. The germination rates of round shape seed were about 0.3~12% higher than those of parallel shape. The germination rates with different storage durations were increased at the level of 25oC and 4oC with the storage days, while they were clearly higher at -20oC compared to those at 4oC and 25oC without the storage durations. With the results of germination rates with treating of plant growth regulator and seed priming, their rates with the round shape seeds were comparatively higher about 6% than those with parallel shape seed and they were increased with increasing of the storage durations. The germination rates were generally appeared higher with GA3 than did those with seed priming. The highest germination rate was appeared at 50 ppm GA3 of plant growth regulator and at -0.5 MPa PEG6000 of seed priming. The germination rates?ㅤ ?ㅤ of Angelica gigas were different with different temperature, storage durations and the treatments of plant growth regulator and seed priming. These results could be used according to different cultivating conditions.

척추 수술시 자가혈액 수혈기를 이용한 자가수혈의 효과

김명호,유문집,유석주,박진영,정홍근,박희곤,문기혁 단국대학교 2000 論文集 Vol.35 No.-

Study Design : Spine fusion with fixation procedure is associated with substantial operative blood loss, which frequently necessitates transfusion. The authors evaluated the safety and efficacy of intraoperative autotransfusion using Cell Saver during spinal surgery. Summary of Literature Review : It has been reported that the contraindications to the use of intraoperative autologous transfusion are malignancy, infection, and gross wound contamination. Objectives : The purpose of this study was to evaluate the safety and efficacy of intraoperative autotransfusion using Cell Saver during spine surgery. Material and Methods : We retrospectively reviewed 62 patients who had operation for lumbosacral spinal stenosis and hypotensive anesthesia. Among them, Group Ⅰwhich received autotrans fusion using Cell Savers included 26 patients with fusion of 2 segments, and Group Ⅱ as control group included 13 patients. Group Ⅲ which received autotransfusion using Cell Savers included 17 patients with function of 3 segments, and Group Ⅳ as control group included 6 patients. We analyzed the amount of intraoperative blood salvage using a Cell Saver, the amount of total homologous transfusion amount, intraoperative blood loss, the amount of postoperative drainage amount and perioperative changes of hemoglobin, hematocrit and platelet count. Results : There was no statistical significance between Cell-Saver group and control group in amount of total homologous transfusion and intraoperative(except in 2 segment of fusion using cell saver: 86.5% increased) & postoperative blood loss(p>0.05). The circulatory balance(amount of intraoperative blood salvage using a Cell Saver plus amount of total homologous transfusion minus amount of total blood loss) was statistical significance between Cell-Saver group and Control group. The Cell-Saver group had positive balance and Control group had negative balance. There were no statistical differance in the evolusion of hemoglobin, hematoocrit and platelet count between Cell-Saver group and Control group(p>0.05). There was no transfusion reaction and thromboembolic disease in all group. Conclusion : In retrospective study, Cell Savers was safe and immediate availability in orthopedic surgery such as spinal surgery. It was effective methods for storing a sufficient volume of blood for spine surgery without affecting the patient's postoperative hemodynamic ststes, too. It was possible to operation without intraoperative homologous blood transfusion in 2 or 3 segment fusion. We may need further study.