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This paper proposes to create solutions to reduce problems associated with supplying clean water and reducing environmental pollution in coastal areas in Korea. It has examined, as a case study, changing social and economic conditions of rural communities in Seochun-Gun, Chungnam Province. It has been found that the region is expected to see exhaustion of clean water resources by continuos water development projects, and contaminations of water quality in costal areas by domestic wastes. The paper has suggested reducing domestic water consumption to reserve resources for clean drinking water and improving sewerage systems to preserve water quality in the coaste in the region.
毒素原性大腸菌의 Enterotoxin에 關한 硏究 : Assay of Escherichia coli heat-labile enterotoxin with the cell culture test(CHO-KI,FL and L-929 cell) and the reserved passive latex agglutination(RPLA) test Reserved passive latex agglutination(RPLA) test 및 細胞培養法 (CHO-KI, FL 및 FL 및 L-929 細胞)에 의한 enterotoxin 檢出
Cell-free culture filtrates of heat-labile enterotoxin producing strain of enterotoxigenic Escherichia coli serotype 0148:H- was detected by use of assays in RPLA test and the cell culture(CHO-KI, FL and L-929 cell) for the production of heat-labile enterotoxin. The media used were CAYE-2, SCD, and Biken No.2 medium. The results obtained were as follfws: 1) FL cell and L-929 cell responded sensitively to the LT enterotoxin, and have given excellent correlation with the CHO-KI cell. CPE in culture was detectable at 17h, increased gradually, reached a maximum at 48 to 72 h. FL cell and L-929 cell will be satisfactory and useful for detecting capable of producing heat-labile enterotoxin in the clinical laboratory . 2) RPLA test is a reliable, and sensitive(capable of detecting 1∼2ng/㎖) assay test for the detection of heat-labile enterotoxin or cholera toxin. In this study, Yields of heat-labile enterotoxin was about 4∼16ng/㎖ in SCD medium and CAYE-2 medium. And, Biken No.2 medium at pH 8.5 produced the highest heat-labile enterotoxin activity (about 32ng/㎖) of the various test media.
The photocatalyst of TiO_2 coated onto glass bead was prepared from sol-gel method. The solids were characterized by X-ray diffraction (XRD), specific surface area (BET), and scanning electron microscopy observation (SEM). The gas-phase photocatalytic degradation of trichloroethylene (TCE) and benzene with coated TiO_2 on glass beads was investigated using a fixed bed reactor. At steady state, conversion yields were obtained for trichloroethylene (ca. 82%) in 400 ppm concentration and benzene (ca. 65%) in 300 ppm. In order to compare the TiO_2 powder for the photocatalytic reaction efficiency, two different powders were used. To obtain higher efficiency, it was the primary factor to select the TiO_2 powder. Through the experiments, the photocatalytic degradation had the behavior of Langmuir-Hinshelwood kinetics as function of reaction time.
The effects of carbon and nitrogen sources on the growth of sodium benzoate-utilizing bacteria, Pseudomonas sp. ATCC 27330, were experimentally investigated in batch culture and the characteristic values were estimated by using the Kono model. The optimun culture conditions for the growth of the strain were 30℃, pH 7.0, sodium benzoate as carbon source and ammonium sulfate as nitrogen source. When the strain was cultivated in 0.08%-sodium benzoate as optimum concentration, the characteristic values estimated by using the Kono model from the data obtained were as follows; the coefficient of growth rate (k), critical cell concentration (Cxc) and theoretical maximum cell concentration (Cxm) were 0.225 h??, 0.123 UOD/ml and 0.235 UOD/ml respectively.
Thirty microorganisms capable of utilizing diaminododecane were isolated from the soil by enrichment culture technique. One of these isolated strains was designated EL-0272S and identified as the genus Brevibacterium from the results of morphological,cultural,and biochemical tests. This isolated strain was named temporaily Brevibacterium sp. EL-0272S. Brevibacterium sp. EL-0272S was tested for ability to utilize different kinds of substituted alkanes containing cyan, amine, chloro, and thiol groups (monoterminally or diterminally substituted) as carbon source. Pentamethylenediamine, hexamethylenediamine, laurylamine, n-decane, and alkane derivatives containing cyan, chloro, and thiol guoups were not utilized by Brebibacterium sp. EL-0272S. The alkane derivatives that did not serve as growth substrates were tested further in oxidation tests using resting cell preparations of Brevibacterium sp. EL-0272S. The most remarkable substrate that was being oxidized was dichlorodecane containing chloro groups diterminally. The metabolic products formed from diaminododecane by Brevibacterium sp. EL-0272S were acid compounds containing carboxyl group and not containing amine group. On the thin layer chromatography, Rf value of these metabolic products was different from that of the products formed by Corynebacterium sp.DAD 2-3. These results suggested the specificity of diaminododecane as carbon source.
The purpose of this study is to analyze synoptically some characteristics of the surface inversion at Kori, southeastern part of Korea and to be useful for a searching examination of the air pollution potenial at a coastal industrial region. The data used in this study are the temperatures at 20m and 1.5m above the surface, wind velocity and wind direction observed hourly at the monitoring station which is located at Gilcheon-ri (N35˚19'44", E129˚17'20") near Kori in Korea, from December, 1967 to November, 1970. By analyzing these data statistically and synoptically, the following results were acquired. 1) The occurence frequency of the surface inversion is the highest in November and the lowest in June. In seasonal variation, autumn has the highest and summer has the lowest occurrence frequency. 2) The modes of the onset and dispersion times of the surface inversion are 18:00 and 08:00 respectively. The annual mean duration of the surface inversion is 11.3 hours. 3) The aspects of annual variations of inversion intensity and occurrence frequency with time are similar to each other and also have a close connection with sunrise, sunset and the changing time of land and sea breezes. 4) According to Pasquill's stabillity classification in inversion cases, it was proved that there are the classes of E, F and G being stable in inversion cases at Kori.
15 Occupational industrial noise levels were investigated and classified into 92 industrial processes. The highest level of noise was found in the mechanical field, ranging from assembling process of 87 dB(A) to rivetting process of 115dB(A). 60 industrial processes out of 92 total processes were investigated. The 66% of them should be under control in terms of dermissible noise exposure. The machine manufacturers, suppliers and importers to install effective noise control equipment should huve the duty on clear information and warning about its danger to hearing, supply of suitable ear protectors, and notice of permissible noise exposure time limits. National Insurance (Industrial Injuries) Scheme, conversion of liability to prove cause and effect in the legal system, regular hearing loss inspection, and education of noise control facilities, were discussed.
The Sinpyeong Crushed Stone Quarry of the Hyeobhwa Aggregate Company faces the estuary of the Nagdong River by the Eulsug Island of the estuarine delta,and is located at Sinpyeongdong, Seo-gu, Busan City. The main workable formation of the quarry is the rhyolite intercalating the layers of the welded lapilli dacite tuff. The acceleration values of the basement vibration are measured by the modified use of the Seismic Timer Model 117 of Dymetric, Inc. in U.S.A. and the Measuring Amplifier of Bruel &Kjoer Inc. in Copenhagen. The explosion methods in the experiment are chiefly the simultaneous blasting, and secondly the millisecond blasting. The vibration-acceleration values of the factory basement of the Hankuk Fiber Glass Co. are less than 8 gals at the explosion of the gelatin dynamite to the amount of 121.5kg, thus the vibration causes no serious nuisance to the factory. As far as the experiments given are concerned, the explosion noises and the crush dusts of the quarry are also within the safety limits in the factory and the residential area. As the crushed stone is of good quality, its abrasion retio is within the level of JISA 5005.
The characteristics of atmospheric factors were investigated using the surface observatory data. In this analysis, we can find that the annual mean temperature, relative humidity, and precipitation show the 14.39℃, 63.3%, and 1178㎜, respectively. The prevailing wind direction is southwestern wind, and the mean wind speed shows 2.7㎧. The amount of cloud is abundant during the summer because of Jang-ma phenomenon and convective cloud induced by terrain effect. The annual mean duration of sunshine represents about 2221 hours.