Application of Artificial Insemination Technology for Dairy Breeding in Mongolia

Hyun‐Tae Jo,Jong‐In Jin,Seong‐Su Kim,Byung‐Hyun Choi,Tumor Baldan,Jung‐Gyu Lee,Yun‐Shik Kim,Sam‐Churl Kim,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

This study was focused on improvement of milk production in Mongolia dairy industry by artificial insemination (AI) technology, of which was supported from ODA project of KOICA in Republic of Korea. The study was started from January 2009 to present and 3rd years in this year. So, all data, especially synchronization and pregnancy of dairy cows (Holstein) will be summarized in final result in this year. For synchronization, total 81 dairy cows selected from 4 private farms that were 38, 30, 8 and 5 in Undarmal milk, Onjin (Enkhbayer), Jargalant, and BRM School, respectively. All the dairy cows were injected intramuscular (IM) of 5 ml PGF2α in the vulva and detected estrus 2 to 3 days after PGF2α injection. Total 78 out of 81 dairy cows (96.3%) were detected estrus by only 1 time injection of PGF2α. The dairy cows that were induced estrus, inseminated with 0.5 ml dairy frozen semen by conventional AI techniques. The pregnancy diagnosis of the AI dairy cows was detected by uterus palpation after 60 days of insemination. Total 75 from 78 inseminated dairy cows (90.1%) were diagnosis pregnant. The estrus induction and pregnant rate were very effective using PGF2α injection and conventional AI techniques in Mongolia dairy cow. The results indicated that AI after estrus induction in Mongolia dairy cows could be applied to dairy breeding technology to improve the breeding efficiency and milk production.

상아질 접착에서 collagenase와 esterase가 미세인장결합강도에 미치는 영향

정영정,현홍근,김영재,김정욱,이상훈,김종철,한세현,장기택 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.2

상아질-레진 접착강도에 대한 collagenase와 esterase의 영향을 살펴보기 위해, 소구치의 교합면 상아질에 Single Bond 2와 Clearfil SE Bond로 접착을 시행하고 미세 시편을 제작하여 PBS, collagenase 용액, esterase 용액에 4주간 보관한 후 미세인장결합강도를 측정, 비교하여 다음과 같은 결론을 얻었다. 1. 모든 보관 용액에서 Single Bond 2의 미세인장결합강도는 Clearfil SE Bond보다 유의하게 낮았다(p<0.05). 2. Single Bond 2의 미세인장결합강도는 collagenase군이 PBS군, esterase군보다 낮았다(p>0.05). 3. Clearfil SE Bond의 미세인장결합강도는 esterase군이 PBS군에 비해 낮았으나(p>0.05), collagenase군보다는 높았다(p>0.05). Collagenase군은 PBS군에 비해 유의하게 낮은 미세인장결합강도를 보였다(p<0.05). The purpose of this study was to evaluate the effect of collagenase and esterase on the microtensile bond strength (μTBS) in dentin bonding. After resin composites were bonded to occlusal dentin, μTBS specimens were formed and stored in PBS, collagenase, or esterase solution. After 4-week storage, μTBS was determined and, the results were as follows: 1. μTBS values of Single Bond 2 were lower than those of Clearfil SE Bond for all storage medium (p<0.05). 2. In single Bond 2 group, collagenase solution lowered bond strength more than PBS and esterase solution (p>0.05). 3. In Clearfil SE Bond group, esterase solution lowered bond strength more than PBS(p>0.05). Collagenase solution lowered bond strength more than esterase solution(p>0.05) and PBS(p<0.05).

Inducing apoptpsis by the inhibition of c-myb in oral squamous carcinoma cell line, KB cell

Lee,Jung-Chang 대한구강생물학회 2007 International Journal of Oral Biology Vol.32 No.4

Oral squamous cell carcinoma (OSCC) is the most commonmalignancy and is a major cause of worldwide cancer mortality.The proto-oncogene c-myb plays an important role in regulationof cell growth and differentiation, and it is expressed at highlevels in hematopoietic cells and many other types of cancers.However, the function of c-myb is not well known in OSCC.The present study aimed to reveal the function of c-myb and totest the alternation of cell growth and signaling by c-myb inOSCC. In this study, c-myb and dominant-negatibe myb(DN-myb) were expressed in an adenovirus-mediated gene deliverysystem to KB cells. The over-expressed c-myb broughtincreased cellular proliferation compared with control cells.However, DN-myb infected KB cells showed significantreduction of cell growth and enhanced induction of apoptosis toactivate PARP and caspase 9. c-myb induced increase of IGF-I,-II and IGF-IR expressions while DN-myb down-regulatedthese expression. Activation of ERK and Akt/PKB pathwaywas shown only in c-myb transduced cells. These findingssuggest that the role of c-myb in cell growth of oral cancer cellsis partially mediated through the modulation of IGFs, ERK andAkt/PKB. From this results, DN-myb is strongly recommendedas a curable gene for the treatment of c-myb dependentmalignancies such as OSCC.

심실 중격 결손에 의한 감염성 심내막염 환자의 치과치료

양정현,현홍근,김영재,김정욱,장기택,이상훈,한세현,김종철 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.3

중독한 추체로 증상을 보인 cyclosporine에 의한 뇌증 1예

강정현,곽승근,신현영,김성은,이정호,이정찬,곽상혁,윤환중,조덕연,김삼용,김제,송창준 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.1

The use of cyclosporine(CsA), a potent immunosuppressive agent, is associated with numerous side effects. Neurotoxicity was earlier less well known, but with growing experience central nervous system side effects are now reported up to 40% of patients. Among various CsA-induced neurotoxicities, tremor is most common and posterior leukoencephalopathy is well characterized. An encephalopathy presenting with pyramidal weakness, however, seems to be rare and poorly documented. We report here a case of encephalopathy presenting with multiple generalized seizures followed by fluctuating quadriparesis developed a few days after starting CsA and resolved spontaneously after withdrawal of the drug . A 48-year-old woman with aplastic anemia was placed upon immmunosuppressive treatment consisting of antithymocyte globulin(ATG), CsA, and methylprednisolone. Two days after starting the therapy, she developed a generalized seizure. The next day she developed 2 more episodes of seizure and the therapy was stopped. Computed tomogram of the brain was normal. Six days after starting therapy, she developed mild motor weakness and paresthesia of both lower extremities, which progressed to a definite quariparesis confining her to a bed in 3 days. Magnetic resonance imaging(MRI) of the brain revealed asymmetric, bilateral high-signal abnormalites in the subcortical white matter of fronto-temporal areas. After reaching to a maximum with some fluactuation(14 days after starting the therapy) the motor weakness started to be resolved. She was discharged with a almost complete resolution of the weakness 45 days after starting the therapy. Five months later she did well and brain MRI was negative.

위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동

이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.

조선업의 작업환경측정결과를 이용한 노출평가의 문제점과 해결방향 : 유사노출군을 중심으로

노영만,임현우,김석일,박효만,정재열,박숙경,김현욱,정치경,이원철,김정만,김수근,고상백,Karl Siber,김은아,최정근 한국산업위생학회 2001 한국산업보건학회지 Vol.11 No.2

Statistical approaches for analysis of data from the limited number of samples in ship building industry(SBI) collected by an industrial hygienist for checking compliance to an occupational standard were considered. Sampling for com-pliance usually has been guided by judgment selection, rather than true randomness, resulting in the creation of compliance samples which approximate a censored sample from the upper tail of the exposure distribution. Similar exposure groups(SEGs) including welding and painting process were established to assess representative values in each groups after reviewing the whole production line in SBI. For the convenient statistical approaches, the code has assigned to each SEGs. The descriptive statistics and probability plotting were used to yield the representative values in each SEGs. In the first step, SEGs of 558 were established from 5 ship building companies, The 38 SEGs showed the uncertainty are divided into each 5 companies and assessed the repre-sentative values again. The 44 SEGs in each companies was not showed the normal and lognormal distribution was analyzed each data. And also, recommendation was suggested to resolve the uncertainty in each groups.

법랑질 표면처리방법에 따른 치면열구전색재의 미세누출에 관한 비교연구

현홍근,김정욱,이상훈 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.3

최근 air-abrasion기술이 새로운 법랑질 표면처리법으로 소개되고 있는데, 이것이 산부식 처리한 것보다 치면열구전색 전처리에 있어 더 나은 결과를 보이는지 연구할 목적으로.저자들은 표면처리법 및 재료에 따른 미세누출도의 차이를 비교해 보고자 하였다. 9개의 군으로 나눈 시편치아에 산부식(1, 4, 7군). air-abrasion(2. 5, 8군). ail·-abrasion후 산부식(3. 6. 9군) 처리한 후, 3종의 치면열구전색재(1∼3군 : Teethmate-F.4∼6군 Ultraseal XT plus, 7∼9군 : DenSeal)로 전색, 중합하여 색소침투도를 관찰하였으며, 각 시편의 전색재를 분리, 주사전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1 표면처리법에 따른 미세누출도는 air-abrasion군, 산부식군 air-abrasion 후 산부식군 순으로 작아졌다(p<0.05) . 단. 7군과 9군간의 유의차는 없었다. 2.미세누출도는 산부식군에서 7군, 4군, 1군, air-abl·asion군에서 8군, 5군, 2군, air-abrasion후 산부식군에서 9군, 3군. 6군 순으로 작아졌으나. 각각 4군과 7군 2군과 5군 3군과 6군간의 유의차는 없었다. 3. SEM상에서, 산부식군은 비교적 긴 레진돌기가 규칙적으로, air-abrasion군은 �裏�레진돌기가 불규칙적으로, air-abrasion후 산부식군에서는 이들이 혼재되어 있는 모습으로 관찰되었다. Recently, the reintroduction of air-abrasion technology in dentistry has added a new potential method of pre-treating teeth prior to placing sealants. The purpose of this in vitro study was to investigate microleakages of several pit and fissure sealants following various surface pre-treatment methods to the enamel , because there was a question concerning the validity of claim that this method was better than the conventional acid etching. Permanent molars were divided randomly into nine groups and treated accordingly: acid etching(group 1, 4, 7), airabrasion(group 2, 5. 8), acid etching after airabrasion(group 3, 6, 9). Then the authors placed and cured the three kinds of sealants(group 1∼3 : Teethmate-F, group 4∼6: Ultraseal XT plus, group 7~9: Denseal) according to the manufacturer's order. They were observed to determine the degree of microleakage . And these specimens were separated from the corresponding enamels and examined by Scanning Electron Microscope. The following results were obtained : 1. In comparing microleakage among tooth surface treatment methods. air-abrasion group (group 2, 5, 8) showed the greatest microleakage. while combination(air-abrasion + acid etching) group showed the least (p<0.05) . However, no significant differences were found between group 7 and 9. 2. The mean microleakages were ranked as follows(p<0.05) : In acid etching groups. group 7> group 4> group 1. In air-abrasive groups. group 8 > group 5 > group 2. In combination groups, group 9 > group 3 > group 6. However. no significant differences were found between group 4 and 7. between group 2 and 5, and be-tween group 3 and 6. 3. SEM showed that comparably longer resin tags were distributed regularly in acid etching groups and that shorter ones irregularly in airabrasion groups. It also showed that these two kinds of tags were distributed simultaneously in combination groups.

장애인 및 노약자를 위한 생활관련 시설의 연계방향

강병근,성기창,박광재,윤영삼,김상운,정현정,류상오 한국의료복지시설학회 2009 의료·복지 건축 Vol.15 No.1

This study aims to plan connecting living facilities considering on the Barrier Free Walking of the disabled and the elderly in the residential area. We had found several physical problems on the peestrian's way that becomes the major factor of disturbing the barrier free movement for using living facilities in the local area by investigations and enquetes. We had suggested the arrangement method for these physical problems on the pedestrian's way and building the ideal area for living facilities by the disposition stages with the result of investigations and enquetes. After these process, we had planned connecting living facilities considering on the Barrier Free WaIking of the disabled and the elderly in the residential area.

인천 인현동 호프집 화재 피해자 분석

최정태,안무업,안희철,최영미,정재봉,서정열,유기철,이삼우,박석현,조준휘,김성환,김아진,송근정 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Purpose : This study was conducted to develop field triage, transportation, distribution, and prehospital care at a fire disaster by analyzing the victims of the fore that broke out at a bar in Incheon. Method : We analyzed the cases of the victims of a fire in Incheon in Oct. 1999. We determined the primary care hospital, the arrival time, the burn size, the outcome, and the injury type from the medical records, the concerned organ records, and interviews with concerned persons. Result : The total number of victims was 137: 56 prehospital deaths, 1 hospital death, and 80 survivals. The Pearson correlation coefficient between the burn size and the severity was -0.175. There were 121(89.6%) cases of inhalation injury, 59 (43.7%) cases of flame burns, 66 (48.9%) cases of hypoxic brain damage, and 16 (11.9%) cases involving other types of injury. Conclusion : The causes of death of the fire victims were inhalation injury and hypoxic brain damage due to CO poisoning and other toxic inhalants. We propose the use of a simple triage and rapid treatment(START) system and a reassessment the delayed category in fire disasters.