정상 한국인에서 연령증가에 따른 혈장 Dehydroepiandrosteronesulfate ( DHEA-S ) 농도 및 DHEA - S / cortisol 비의 변화

박성우,유형준,임성희,최문기,유재명,박민숙,이권엽,박철수,김철홍,김현규,김두만,박규용 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.2

Backgroud: DHEA-S is the most abundant steroid hormone in circulation, and primarily secreted from the adrenal cortex, but its physiological role is little known. One of the characteristic features of DHEA-S is progressive decrement of plasma DHEA-S level with advancing age, in contrast, plasma levels of other adrenal hormones are not chaging or littie decreasing. To grasp the trends of plasma DHEA-S level and DHEA-S/cortisol ratio by age in healthy Korean, we measured the plasma DHEA-S levels and DHEA-S/cortisol ratios in healthy Korean. Methods: Healthy Korean(men: 99, women: 102, age range: 15-97 year old)were studied. Subjects were not taking drugs(such as glucocorticoid or androgenic medication) or cigarettes known to modify the plasma level of DHEA-S and cortisol, and had no evidence of hepatic, renal disease or hyperlipidemia as determined by serum lipid, bilirubin, SGOT, SGPT, BUN, creatinine. Data were analyzed by 10-year age group for men and women: i.e, 10-19, 20-29, 30-39, 40- 49, 50-59, 60-69, 70-79, 80-89 and 90 year or more. Plasma DHEA-S levels were measured by using a commercially available RIA kit with 125I labeled-DHEA-SO4(Coat-A Count DHEA-SO4), and for the measurement of plasma cortisol levels, commercial Gamma Coat TM[125I] Cortisol Radioimmunassay Kit was used. Results:. 1) In both men and women, plasma DHEA-S level showed high interindividual variation within the same age group. 2) There were individual sex differences in plasma levels of DHEA-S, in all age groups, plasma DHEA-S levels were significantly higher values for men than for women. 3) Maximum plasma DHEA-S levels(men; 237+-3.35 ug/dL, women; 108+-17.5 ug/dL) were at third decade in both men and women. 4) Both men and women showed the continuous decline in plasma DHEA-S level with age. These age-related decline was more prominent in men than in women(men; y= -3.152 * +292.6, r2= 0.8459, P$lt;0.05, women; y= -1.417 * +143.3, r2 = 0.7278, P$lt; 0.05). 5) As an index of aging, there was no stastical difference between DHEA-S and DHEA- S/cortisol ratio. Conclusion: In healthy Korean, there were high interindividual variation of plasrna DHEA-S levels. In both men and women plasma DHEA-S level was peak at third decade, and from when it declined progressively with age. These results suggest that although the reliability of single plasma DHEA-S measurement are limited, the decline of DHEA-S with advancing age might be a specific marker of endocrinologic hormonal milieu(aging index). Also, concerning to individual adrenal secreting capacity, we measured DHEA-S/cortisol ratio. But we did not found that plasma DHEA-S/cortisol ratio is superior to the plasma DHEA-S level as an aging index. (J Kor Soc Endocrinol 12:245-254, 1997)

제2형 당뇨병 환자의 치료에서 복약순응도가 혈당 조절에 미치는 영향

김경선,김민정,박소현,박영주,허정순,이은경,김선영,이수형,김상수,강양호,손석만,김인주,김용기 한국병원약사회 2009 병원약사회지 Vol.26 No.2

Abstract: As known in clinical trial like DCCT, UKPDS, tight glycemic control is essential to prevent complications from diabetes mellitus(DM). In type 2 diabetes taking multiple medication, nonadherence to medications is common problem. The aim of our study is to investigate adher-ence rate of patients with type 2 DM and to evaluate association between many factors including adherence rate and HbA_(1C). 441 patients with type 2 DM who visited Busan National University Hospital endocrine internal department from Aug 13 to Sep 2, 2008(3weeks) were enrolled. We conducted a man-to-man questionnaire survey about general knowledge about diabetes mellitus, medicine and adherence, and measured HbA_(1C). Among them, 305 patients taking oral hypo-glycemic agent(OHA) were analyzed to investigate correlation between adherence rate and glycemic control. We studied 305 patients(male 126, female 179, average age 61.2±9.8 years, DM duration 8.6±6.4 years, average adherence 88.8±16.2%, average HbA_(1C) 7.03± 1.18%). HbA_(1C) showed significant relation with DM duration(r=0.137, p=0.017), number of aHA in regimen (r=0.135, p=0.018), dosage frequency(r=0.132, p=0.026), Medication Refill Adherence(MRA)(r=-0.124, p=0.030) and adherence(r=-0.168, p=0.003). Adherence had significant relation with MRA(r=0.148, p=0.010) but didn't have statical corelationship with DM duration, number of aHA and dosage frequency. Patients that their adherence rate exceed 90% took fewer OHA(p=0.011) and went on a better dietary treatment(p=0.007). In patients controlling diabetes mellitus with aHA, when adherence rate and MRA was higher, HbA_(1C) was lower. As a result, to improve adher-ence rate, it is necessary to enhance patient education, do careful consideration about prescrip-tion medicine number and dosage frequency

간 세포암에서 VEGF, TGF-β1, b-FGF 발현의 의의

김성용,남충현,주종우,채만규,백무준,이문수,김형철,안현철,김홍수,김창진,김창호 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: Angiogenesis is important for the proliferation and the metastasis of solid tumors. The growth of a solid tumor is widely recognized to depend on the process of neovascularrozation. Without angiogenesis, tumors cease to grow beyond even a few milimeters in diameter. It has been shown that tumor vascular density is an independent prognostic marker in several types of human tumors and is known to correlate with poor prognosis. To date, many angiogenic factors have been identified, such as transforming growth factor-α(TGF-α), transforming growth factor-β(TGF-β), fibroblast growth factor family(FGF), vascular endothelial growth factor(VEGF), platelet derived endothelial cell growth factor(PD-ECGF), tumor necrosis factor-α(TNF-α), and angiogenin. Hepatocellular carcinoma(HCC) is the second most common tumor in Korean males and is known as a typical hypervascular tumor with frequent portal vein invastion. The authors identified the expreesion of VEGF, TGF-β1, and b-FGF in HCC specimens and evaluated the relationship between these growth factors and the clinicopathologic characteristics of HCC. Method: We reviewed the medical records of 30 patients who were diagnosed as hepatocellular carinoma treated with hepatic resection between January 1994 and December 1998 in Soonchunhyang University Chunan Hospital. The selection of the cases was decided according to the condition of paraffin block fixation. The prognostic factors such as age, sex, tumor size, concentration of serum α-fetoprotein, presence of liver cirrhosis, presence of tumor emboli in portal vein, TMN stage, amount of transfusion during the operation, hepatitis B virus(HBV) infection, and Edmonson-Steiner(E-S) grade were investigated. Relationship between the prognostic factors and the immunopathologic expression of the TGF-β1, b-FGF, and VEGF was examined. Result: Thirty patients (24 males, 6 females) were included in the current study. The patient's mean age was 50.6 years and the age ranged from 36 to 65 years. The mean size of the tumor was found to be 5.2cm. All the patients were follewed up for 7 to 63 months. Child's classification A patients were 23(76.7%)cases, B patients were 7(23.3%)cases, and C was none. Immunohistochemical staining of HCC tumor mass in VEGF expression patients were 17(56.7%), b-FGF expression patients were 10(33.3%), and TGF-β1 expression patients were 10(33.3%). VEGF expression or more than one positive expression among the three factors correlated with tumor size and the stage of HCC but did not correlated with other clinicopathological characteristics. TGF-β1 and b-FGF did not correlate with any clinicopathological characteristics. Conclusion: The results suggest that the expression of VEGF or more than one positive expression among the three factors in HCC cells may be a significant prognostic factor of HCC.

흰쥐 해마박편에서 veratrine과 고농도 포타슘자극시 칼슘이온이 gamma-aminobutyric acid 유리에 미치는 영향에 관한 연구 : A role of calcium

강수만,김형룡,김관식,정동균 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2

Present study was performed to clarify the effect of calcium on the release of gamma-aminobutyric acid (GABA) employing hippocampal slices. Hippocampal slices(300-400㎛ thick) were prepared by the method of Kim et al(1988) and pre-equilibrated in Krebs-bicarbonate medium(KBM, pH 7.4) for 1hr at 37℃. In case of veratrine-induced GABA release, pre-equilibrated slices were incubated in fresh KBM and then veratrine (25μM)-containing KBM for 10min period in the presence or absence of 2.5mM Ca^2+. In case of potassium-induced GABA relaese, pre-equilibrated slices were incubated in fresh KBM and then potassium(50mM)-containing KBM for 5min period in the presence or absence of 2.5mM Ca^2+. Basal and veratrine and potassium-induced release of GABA was determined from recovered medium by HPLC. The observed results were as follows: 1. The release of GABA induced by the 10min-exposure of 25μM veratrine and 5min-exposure of 50mM potassium in the presence of 2.5mM Ca^2+ was 228.9±11.2 nmol and 100.1±8.9nmol, respectively. When compared with released amounts of GABA during the corresponding spontaneous periods, these were 6.8 and 4.6-fold increase respectively. 2. The release of GABA induced by the 10min-exposure of 25μM veratrine and 5min-exposure of 50mM potassium in the absence of Ca^2+ was 381.4±30.2 nmol and 55.1±4.1 nmol, respectively. When compared with released amounts of GABA during the corresponding spontaneous periods, these were 11.3 and 2.4-fold increase respectively.

The Assessment of Exposure Dose of Radiation Workers for Decommissioning Waste in the Radioactive Waste Inspection Building of Low and Intermediate-Level Radioactive Waste Disposal Facility

Rin-Ah Kim,Ho-Seog Dho,Tae-Man Kim,Chun-Hyung Cho 한국방사성폐기물학회 2020 방사성폐기물학회지 Vol.18 No.2(E)

한국원자력환경공단은 처분시설 내 1단계 인수·저장구역의 인수검사 공간 및 드럼 취급 공간 부족에 대한 문제를 해결하기 위하여 방폐물검사건물을 건설하여 저장·처리능력을 확충할 예정이다. 본 연구에서는 MCNP 코드를 이용하여 방폐물검사 건물 내 저장구역에서 취급하는 해체 방사성폐기물 대상 신형처분용기를 대상으로 작업종사자의 피폭선량을 평가하였다. 평가결과, 시설 내 저장 가능한 최대 용기 개수(304개)와 방사선작업에 대한 연간 예상 작업시간(약 306시간)에 대하여 연간 집단선량은 총 84.8 man-mSv로 계산되었다. 시설 내 총 304개의 신형처분용기(소형/중형 타입)가 저장 완료된 시점에서 인수검사, 처분검사를 위한 작업종사자의 투입인력은 총 25명, 작업종사자 당 예상피폭선량은 연평균 3.39 mSv로 산출 되었다. 소형용기 취급 시 작업종사자의 고방사선량 작업에 따른 작업효율과 방사선적 안전성 확보를 위해서는 콘크리트 라이너의 두께를 증가시키는 추가적인 차폐가 필요할 것으로 평가되었다. 향후 본 연구를 바탕으로 실측기반의 해체폐기 물의 선원항과 특성을 활용하여 방사선작업 당 작업시간 및 투입인력을 산출함으로써 작업종사자의 최적의 방사선작업조건을 도출할 수 있을 것으로 사료된다. The Korea Radioactive Waste Agency plans to expand the storage capacity of radioactive waste by constructing a radioactive waste inspecting building to solve the problem of the lack of inspection space and drum-handling space in the radioactive waste receipt and storage building for the first-stage disposal facility. In this study, the exposure doses of radiation workers that handle new disposal containers for decommissioning waste in the storage areas of the radioactive waste inspecting building were calculated using the Monte Carlo N-particle transport code. The annual collective dose was calculated as a total of 84.8 man-mSv for 304 new disposal containers and an estimated annual 306 working hours for the radiation work. When the 304 new disposal containers (small/medium type) were stored in the storage areas, it was found that 25 radiation workers should be involved in acceptance/disposal inspection, and the estimated exposure dose per worker was calculated as an average annual value of 3.39 mSv. When the radiation workers handle the small containers in high-radiation dose areas, the small containers should be shielded further by increasing the concrete liner thickness to improve the work efficiency and radiation safety of the radiation workers. The results of this study will be useful in establishing the optimal radiation working conditions for radiation workers using the source term and characteristics of decommissioning waste based on actual measurements.

다이나믹 프로그래밍을 이용한 궤도의 최적 재돌입

김중완,이만형 동아대학교 공과대학 부설 한국자원개발연구소 1985 硏究報告 Vol.9 No.1

The optimal re-entry from orbit problem presents some very interesting problems to be solved. A dynamic programming solution is very well suited for the problem compared to a two point boundary value with 8 state and costate equations using the minimum principle. The dynamic programming solution is also more attractive than a brute force grid type search method. Even so, the problem presented here requires more work and computer time than an end of the study allows.

Stage II B형 골육종에 대한 술전 항암화학요법 및 수술적 치료 결과

김정만,우영균,김형민,김훈교,이승구,강용구 가톨릭중앙의료원 가톨릭암센터 1997 암심포지움 Vol.- No.1

Recent advances in imaging techniques, surgery and combination anti-cancer chemotherapy have brought high survial rates in osteosarcoma. To investigate the survival rate, local recurrence and complications in treatment, we analysed 25 osteosarcoma cases who had been treated with preoperative neo-adjuvant chemotherapy, surgery and post operative chemotherapy at Department of Orthopedic Surgery, Catholic University. From May 1988 to April 1995, 42 cases of stage IIB osteosarcoma were admitted in Department of Orthopedic surgery. Among them, 17 cases who didn't follow our treatment guidance were excluded in this study. The average age were 19 years. There were 21 males and 4 females. The involved sites were 4 humerus, 10 femur, 10 tibia and 1 talus. Eleven cases had received intra-arterial cisplatin and intravenous adriamycin chemotherapy, and 7 T-10 protocol and 7 intravenous ifosfamide, ADR, methotrexate, cisplatin. Among twenty-three cases were treated with limb salvage surgery, and 2 amputation. The average follow-up was 35 months(3-82). There were 14 cases of continuous disease free, 9 cases of died of desease, 1 case of alive with disease, and 1 case of no evidence of disease at final follow-up. There were three cases of local recurrence at 6,8 and 12 months after operation. The Estimated kaplan-Meier's 5 year survival rates for all, ADR-cisloatin group, T-10 protocol group, and ifosfamide regimen group were 66%, 73%, 44% and 72%, respectively.

호흡성 분진중 석영분석시 FTIR-직접필터법의 현장적용 가능성 연구

김형준,피영규,노영만,김현욱 한국산업위생학회 2002 한국산업보건학회지 Vol.12 No.2

To study if the fourier transform infrared spectrophotometry(FTIR)-direct on filter(DOF) can be utilized for estimation quartz content in airborne respirable dusts from various manufacturing industries, analytical results were compared with those of the traditional KBr transfer (NIOSH 7602) methods. Respirable dust samples were collected using the 10mm Dorr-Oliver nylon cyclone equipped with a 25mm cassette. 30 area samples were collected from the stone, foundry, concrete and brickmaking factories. Quartz content was estimated using the intensity of the absorption peak of quartz at 799 cm_-1 by a FTIR. Respirable dust concentrations ranged from 0.25 to 1.25㎎/㎥, and were highest at the stone treating factory. Quartz contents quantified by the transfer method were higher than those of by DOF method for samples from the foundry, brick, and concrete factories. No statistically significant difference of quartz content in samples from the stone factory was found. The results of the study suggest that, although possible to use the FTIR-DOF method as a suitable substitute for analyzing quartz content, it is necessary to correct the influence of the interfering minerals in order to adopt the FTIR-DOF for routine analytical tool.

혈중 및 호기 일산화탄소를 이용한 일산화탄소 헤모글로빈 농도간의 관련성 연구

김형수,박태혁,손지언,정갑열,김정만,김준연 동아대학교 2001 大學院論文集 Vol.26 No.-

This study was conducted to find a correlation between blood COHb and calculated COHb with expiratory air and related variables such as occupational and smoking factors. Author selected 69 healthy workers in 8 workplaces suspected CO exposure. Basic informations were collected by self-reported questionnaire and medical history taking. Author measured Indirect COHb concentration with expiratory air using Micro II-Smokerlyzer and blood COHb concentration using Oxymeter. The results of the study were summarized as follows : 1. In the smoker, mean blood COHb and calculated COHb with expiratory air were 2.21±0.98% and 2.27±0.97 %, and there was not a statistically significant difference. In the non-smoker, mean blood COHb and calculated COHb with expiratory air were 0.42±0.43 % and 0,65±0.30 %, and there was also not a statistically significant difference. 2. There were positive correlations between blood COHb and calculated COHb with expiratory air in both the smoker (r=0.91, p=0.0001) and non-smoker (r=0.73, p=0.0001). 3. In the smoker, the factors affected COHb were total smoking index (Pack × years) and passed time after the last smoking. 4. In the nonsmoker, the parking and driving groups had higher COHb concentration than others (p<0.05). In conclusion, there was no significant difference between indirect COHb concentration with expiratory air using Smokerlyzer and blood COHb concentration and there was a highly significant correlation between indirect and blood COHb concentrations. Therefore indirect COHb concentration with expiratory air is a useful screening method for CO exposure. Author suggest the resection of smoking during at least 90 minutes before measuring COHb concentration in order to exclude smoking effects.

된장으로부터 혈전용해효소 생산균주 분리

김진만,서형주 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

Bacterial strains showing the fibrinolytic activity were screened from Doenjang (Korean traditional soybean paste). The strain 5,6, 13 and 15 isolated from Doenjang showed a high level of caseinolytic and fibrinolytic activity. The fibrinolytic activities of strain 5, 6, 13, and 15 were 32.9, 31.0, 22.3, and 23.2 unit, respectively. A strain 5 among the four strains showed the highset fibrinolytic activity (32 unit), and subsequently identified as Bacillus subtilis. The fibrinolytic strain was designated as Bacillus subtilis KS-5.